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The map label for this gene is pheS

Identifier: 212223542

GI number: 212223542

Start: 368058

End: 369560

Strand: Reverse

Name: pheS

Synonym: TON_0396

Alternate gene names: 212223542

Gene position: 369560-368058 (Counterclockwise)

Preceding gene: 212223543

Following gene: 212223541

Centisome position: 20.0

GC content: 53.23

Gene sequence:

>1503_bases
ATGGAGCTTAGCTATCAAGAGAAGCTGACGCTCATAAAACTTAACGAGTTGAAAAAGGCTAAATTCGACGAGCTCGTTGA
AAAAACCGGTCTCGACCAGGTCGCGGTCATGAGAGCCGTTCTCTGGCTTCAGAGCAAAGGTCTGGCGAGGCTCCACGAGA
AGAGCGAGAGGATAGCAAAGCTAACCGAGACCGGTAAGAAATATGCTCAAATTGGACTGCCAGAATGGAGGGCTCTGAAA
GTCCTCAGGAAAAAGGGCAAGGTCACACTCGATGACCTCAAAGAAGTCCTCAGCGAGGACGAGCTGAAGCCGATAGTCGG
CCTCCTCAGGAAGGAGGGCTGGGCGAGCATTAGGAAGGAGGATGGAAAGCTCGTCCTCGAGATAACGGAGAATGGCCTTA
AAGCCGAGGAGAGAGCGATTGACAGGGCACTAAAGCTCCTCGCCGAGAAAGAAGCTATTCCCGTCAGTGAGATTGAGGAA
ATTATCAAGGTGAACGACCTCAAGAGAAGGAAGATAGCCGAAGAAGACATCGTAACCGAAAGAACCGTCGAGATAACGCC
CGAAGGAGAGGAGCTCGCAAATAAAGGCCTTGAGCTCAAGGAGGAAGTCTCAACCCTCACGCCGGAGCTCATAAAGTCCG
GAAAGTGGCGTGAGGTCGAGTTCAAGCGCTTCAACATCCAGGCGCCGGTGAGAAGGATTTATCCCGGCAAGAAGCAGCCC
TACAGGGCATTCCTCGACAAGATAAGGAGAAGGCTCATAGAGATGGGCTTCATCGAAATGACTGTTGACAGCCTCATAGA
GACGCAGTTCTGGAACTTCGACGCTCTCTTCCAGCCTCAGAACCATCCGGCAAGAGAATGGACAGACACTTATCAGCTTA
AATATCCGAAAAGCGGCTACCTACCCAATAAAGAACTCGTCGAGAGGGTTAAGACCGCCCACGAGAGAGGCTTAGCTGGC
TCACGCGGCTGGGGCTACGTCTGGAGTCCGGAGAGAGCCATGTTGCTGATGCCGAGAGCCCACGGTACCGCCCTAAGCGG
CAGGCAGCTTGCTAAAGGTGTCGAGATTCCGGGCAAATACTTCACGATACAGCGCGTTTTCAGGCCCGACGTCCTGGACA
GGACGCACCTCATAGAATTCAACCAGGTGGACGGTTTCGTGGTTGGTGAAGACCTCAACTTCAGGCATCTCTTGGGTATA
CTCAAGCGCTTCGCCGTTGAGATCGCCGGAGCCAAGAAAGTCAAGTTCCTGCCGGACTATTATCCGTTCACCGAGCCGAG
CGTACAGATGAGCGCCTACCATCCCGAGCTCGGCTGGGTCGAGTTCGGCGGCGCTGGAATATTCAGAGAGGAAATGACGA
GGGCCCTAGGCATAGATGTTCCTGTAATAGCGTGGGGAATTGGAATTGACAGACTTGCCATGTTCAAACTCGGAATCGAC
GACATAAGGTATCTCTTCAGCTACGACCTGGGGTGGCTGAGAGAGGCGAGGCTGGTCTGGTAA

Upstream 100 bases:

>100_bases
AGGTCGTCTTCTTCCCCCACAAGAAGTGAGTTTTCCTTATTTTTCTTTCACCGCAATTCTTAAGTAACTCTTCTCAAACT
CAACCCAAGGAGGTGGCCCT

Downstream 100 bases:

>100_bases
AGGGGTGATGCTCATGCCGAAGTTCGATGTTTCGAAAGCGGATTTAGAGAGGCTCGTCGGAAAGAGCTTCACCGTTGAGG
AATGGGAAGACCTTTTCCTC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MELSYQEKLTLIKLNELKKAKFDELVEKTGLDQVAVMRAVLWLQSKGLARLHEKSERIAKLTETGKKYAQIGLPEWRALK
VLRKKGKVTLDDLKEVLSEDELKPIVGLLRKEGWASIRKEDGKLVLEITENGLKAEERAIDRALKLLAEKEAIPVSEIEE
IIKVNDLKRRKIAEEDIVTERTVEITPEGEELANKGLELKEEVSTLTPELIKSGKWREVEFKRFNIQAPVRRIYPGKKQP
YRAFLDKIRRRLIEMGFIEMTVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPKSGYLPNKELVERVKTAHERGLAG
SRGWGYVWSPERAMLLMPRAHGTALSGRQLAKGVEIPGKYFTIQRVFRPDVLDRTHLIEFNQVDGFVVGEDLNFRHLLGI
LKRFAVEIAGAKKVKFLPDYYPFTEPSVQMSAYHPELGWVEFGGAGIFREEMTRALGIDVPVIAWGIGIDRLAMFKLGID
DIRYLFSYDLGWLREARLVW

Sequences:

>Translated_500_residues
MELSYQEKLTLIKLNELKKAKFDELVEKTGLDQVAVMRAVLWLQSKGLARLHEKSERIAKLTETGKKYAQIGLPEWRALK
VLRKKGKVTLDDLKEVLSEDELKPIVGLLRKEGWASIRKEDGKLVLEITENGLKAEERAIDRALKLLAEKEAIPVSEIEE
IIKVNDLKRRKIAEEDIVTERTVEITPEGEELANKGLELKEEVSTLTPELIKSGKWREVEFKRFNIQAPVRRIYPGKKQP
YRAFLDKIRRRLIEMGFIEMTVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPKSGYLPNKELVERVKTAHERGLAG
SRGWGYVWSPERAMLLMPRAHGTALSGRQLAKGVEIPGKYFTIQRVFRPDVLDRTHLIEFNQVDGFVVGEDLNFRHLLGI
LKRFAVEIAGAKKVKFLPDYYPFTEPSVQMSAYHPELGWVEFGGAGIFREEMTRALGIDVPVIAWGIGIDRLAMFKLGID
DIRYLFSYDLGWLREARLVW
>Mature_500_residues
MELSYQEKLTLIKLNELKKAKFDELVEKTGLDQVAVMRAVLWLQSKGLARLHEKSERIAKLTETGKKYAQIGLPEWRALK
VLRKKGKVTLDDLKEVLSEDELKPIVGLLRKEGWASIRKEDGKLVLEITENGLKAEERAIDRALKLLAEKEAIPVSEIEE
IIKVNDLKRRKIAEEDIVTERTVEITPEGEELANKGLELKEEVSTLTPELIKSGKWREVEFKRFNIQAPVRRIYPGKKQP
YRAFLDKIRRRLIEMGFIEMTVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPKSGYLPNKELVERVKTAHERGLAG
SRGWGYVWSPERAMLLMPRAHGTALSGRQLAKGVEIPGKYFTIQRVFRPDVLDRTHLIEFNQVDGFVVGEDLNFRHLLGI
LKRFAVEIAGAKKVKFLPDYYPFTEPSVQMSAYHPELGWVEFGGAGIFREEMTRALGIDVPVIAWGIGIDRLAMFKLGID
DIRYLFSYDLGWLREARLVW

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 2 subfamily

Homologues:

Organism=Homo sapiens, GI4758340, Length=484, Percent_Identity=34.297520661157, Blast_Score=247, Evalue=2e-65,
Organism=Escherichia coli, GI1788007, Length=277, Percent_Identity=29.2418772563177, Blast_Score=108, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI32563657, Length=470, Percent_Identity=33.6170212765957, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI17508957, Length=470, Percent_Identity=33.6170212765957, Blast_Score=246, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321087, Length=441, Percent_Identity=32.6530612244898, Blast_Score=225, Evalue=2e-59,
Organism=Drosophila melanogaster, GI18858079, Length=335, Percent_Identity=38.8059701492537, Blast_Score=211, Evalue=1e-54,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_THEON (B6YTJ4)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306778.1
- GeneID:   7016691
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0396
- HOGENOM:   HBG497668
- OMA:   PEFYQCE
- ProtClustDB:   PRK04172
- GO:   GO:0005737
- HAMAP:   MF_00282
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR022917
- InterPro:   IPR002319
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF01409 tRNA-synt_2d

EC number: =6.1.1.20

Molecular weight: Translated: 57701; Mature: 57701

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSYQEKLTLIKLNELKKAKFDELVEKTGLDQVAVMRAVLWLQSKGLARLHEKSERIAK
CCCCHHHCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LTETGKKYAQIGLPEWRALKVLRKKGKVTLDDLKEVLSEDELKPIVGLLRKEGWASIRKE
HHHHHHHHHHCCCCHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DGKLVLEITENGLKAEERAIDRALKLLAEKEAIPVSEIEEIIKVNDLKRRKIAEEDIVTE
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RTVEITPEGEELANKGLELKEEVSTLTPELIKSGKWREVEFKRFNIQAPVRRIYPGKKQP
CEEEECCCHHHHHHCCCCHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCH
YRAFLDKIRRRLIEMGFIEMTVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPKSGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEECCCCCC
LPNKELVERVKTAHERGLAGSRGWGYVWSPERAMLLMPRAHGTALSGRQLAKGVEIPGKY
CCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEECCCCCCCCCHHHHCCCCCCCCE
FTIQRVFRPDVLDRTHLIEFNQVDGFVVGEDLNFRHLLGILKRFAVEIAGAKKVKFLPDY
EEHHHHHCCCCCCCHHEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
YPFTEPSVQMSAYHPELGWVEFGGAGIFREEMTRALGIDVPVIAWGIGIDRLAMFKLGID
CCCCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCHHEECCCHHHHHHHHHCHH
DIRYLFSYDLGWLREARLVW
HHHHHHHHCHHHHHHHCCCC
>Mature Secondary Structure
MELSYQEKLTLIKLNELKKAKFDELVEKTGLDQVAVMRAVLWLQSKGLARLHEKSERIAK
CCCCHHHCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LTETGKKYAQIGLPEWRALKVLRKKGKVTLDDLKEVLSEDELKPIVGLLRKEGWASIRKE
HHHHHHHHHHCCCCHHHHHHHHHHCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCC
DGKLVLEITENGLKAEERAIDRALKLLAEKEAIPVSEIEEIIKVNDLKRRKIAEEDIVTE
CCCEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
RTVEITPEGEELANKGLELKEEVSTLTPELIKSGKWREVEFKRFNIQAPVRRIYPGKKQP
CEEEECCCHHHHHHCCCCHHHHHHHCCHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCH
YRAFLDKIRRRLIEMGFIEMTVDSLIETQFWNFDALFQPQNHPAREWTDTYQLKYPKSGY
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCCEEEECCCCCC
LPNKELVERVKTAHERGLAGSRGWGYVWSPERAMLLMPRAHGTALSGRQLAKGVEIPGKY
CCCHHHHHHHHHHHHHCCCCCCCCCEEECCCCEEEEEECCCCCCCCCHHHHCCCCCCCCE
FTIQRVFRPDVLDRTHLIEFNQVDGFVVGEDLNFRHLLGILKRFAVEIAGAKKVKFLPDY
EEHHHHHCCCCCCCHHEEEEECCCCEEECCCCCHHHHHHHHHHHHHHHCCCCCEEECCCC
YPFTEPSVQMSAYHPELGWVEFGGAGIFREEMTRALGIDVPVIAWGIGIDRLAMFKLGID
CCCCCCCCEEEEECCCCCCEEECCCCHHHHHHHHHHCCCCCHHEECCCHHHHHHHHHCHH
DIRYLFSYDLGWLREARLVW
HHHHHHHHCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA