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The map label for this gene is cpsA1 [H]

Identifier: 212223494

GI number: 212223494

Start: 322554

End: 323702

Strand: Reverse

Name: cpsA1 [H]

Synonym: TON_0348

Alternate gene names: 212223494

Gene position: 323702-322554 (Counterclockwise)

Preceding gene: 212223497

Following gene: 212223481

Centisome position: 17.52

GC content: 53.87

Gene sequence:

>1149_bases
ATGAACCCCATTGAAGAGGCCCTGAAAATTAAAGACGAGATAATAGCCTGGAGAAGAGACTTTCATATGCATCCAGAGCT
AAAATACGAAGAGGAACGGACTTCCAGAATCGTCGAGGAGCACCTTCGTGAGTGGGGATACAAAATAAAGCGCGTCGGCA
CTGGGATAATAGGGGACATCGGCGAGGGAGAGAAGACTATAGCCCTCCGCGCGGATATGGACGCTCTGCCTGTCCAGGAA
GAGAACGACGTTCCCTACAGGTCGAGAATTCCCGGAAAGATGCACGCCTGCGGCCACGATGCTCACACTGCCATGCTCCT
CGGGGCGGCGAAGATAATAGCAGAGCATGCGGACGAGCTGGGTGGAAAGGTTCGCCTAATCTTCCAGCCGGCAGAGGAAG
GTGGCAACGGGGCACTGAAGATGATAGAGGGAGGAGCTCTCGATGGAGTCGATGCCATCTTTGGCTTCCACGTCTGGATG
GATCTGCCCAGCGGAATAATAGGCATCAGAGACGGACCGTTTTTGGCAGGAGCTGGCTTTTTCGAAGCGAAGGTAATCGG
AAAGGGCGGACATGGTGCTTCTCCCCACGAGGCAATTGACCCCATACCAATAGCCGCTGAAACCGTTCTGGCCCTTCAGA
CGATAGTGAGCAGGAACGTTAACCCAATCGAGACGGGAGTTGTTAGCGTTACGGCCATAAACGGCGGGACAACCTTCAAC
GTTATTCCCGAGGAAGTTACCCTAAAAGGAACCTTCCGCTACTACAAGCCAGAGGTTGGAGAGATGATAAAGAAAAGGAT
GGCCGAGATAATAGAAGGCGTTGCCAAAACCCATGGCGCACGAGCCGAGTTCAGCATCAACGACCTCGTTCCGCCAACGA
TAAACGACAAGGCAATGGCGGACTTCGCGAGAAAGGTGGCCGAGAAGTACCGCCTCAGGCACGGCGATGTCGCGATGTCG
ATGGGCGCAGAAGACTTTGCCTACTATCTCCAGAGGGTTCCGGGGGCTTTCTTGGCGCTTGGAATAAGGAACGAGGGGAA
GGGAATAGTTCATCCCCACCACCATCCGAAGTTCGACGTTGATGAGGACGTCCTTCACCTCGGAACAGCGATGGAAGTGG
CCTTGGCGTTTGAGTTTCTGAAGGATTAA

Upstream 100 bases:

>100_bases
CGTAATCACCTACCAGGGTGGATTTATAAATTTTTTGGTCTCTTTAGATGGGCATCGAAAAGCTAATATGGGTGTTTGTC
GAGGTTGATGCGGTGATACC

Downstream 100 bases:

>100_bases
TGCCCCAATCTTTTCCCAATTATTGGGACGTCCCCCTTCTTTTCCGCATCCAGCGCATCCGCCATGTCTTCATCGAGCAC
TGGCCCCTCACAGAAAGACC

Product: bifunctional carboxypeptidase/aminoacylase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MNPIEEALKIKDEIIAWRRDFHMHPELKYEEERTSRIVEEHLREWGYKIKRVGTGIIGDIGEGEKTIALRADMDALPVQE
ENDVPYRSRIPGKMHACGHDAHTAMLLGAAKIIAEHADELGGKVRLIFQPAEEGGNGALKMIEGGALDGVDAIFGFHVWM
DLPSGIIGIRDGPFLAGAGFFEAKVIGKGGHGASPHEAIDPIPIAAETVLALQTIVSRNVNPIETGVVSVTAINGGTTFN
VIPEEVTLKGTFRYYKPEVGEMIKKRMAEIIEGVAKTHGARAEFSINDLVPPTINDKAMADFARKVAEKYRLRHGDVAMS
MGAEDFAYYLQRVPGAFLALGIRNEGKGIVHPHHHPKFDVDEDVLHLGTAMEVALAFEFLKD

Sequences:

>Translated_382_residues
MNPIEEALKIKDEIIAWRRDFHMHPELKYEEERTSRIVEEHLREWGYKIKRVGTGIIGDIGEGEKTIALRADMDALPVQE
ENDVPYRSRIPGKMHACGHDAHTAMLLGAAKIIAEHADELGGKVRLIFQPAEEGGNGALKMIEGGALDGVDAIFGFHVWM
DLPSGIIGIRDGPFLAGAGFFEAKVIGKGGHGASPHEAIDPIPIAAETVLALQTIVSRNVNPIETGVVSVTAINGGTTFN
VIPEEVTLKGTFRYYKPEVGEMIKKRMAEIIEGVAKTHGARAEFSINDLVPPTINDKAMADFARKVAEKYRLRHGDVAMS
MGAEDFAYYLQRVPGAFLALGIRNEGKGIVHPHHHPKFDVDEDVLHLGTAMEVALAFEFLKD
>Mature_382_residues
MNPIEEALKIKDEIIAWRRDFHMHPELKYEEERTSRIVEEHLREWGYKIKRVGTGIIGDIGEGEKTIALRADMDALPVQE
ENDVPYRSRIPGKMHACGHDAHTAMLLGAAKIIAEHADELGGKVRLIFQPAEEGGNGALKMIEGGALDGVDAIFGFHVWM
DLPSGIIGIRDGPFLAGAGFFEAKVIGKGGHGASPHEAIDPIPIAAETVLALQTIVSRNVNPIETGVVSVTAINGGTTFN
VIPEEVTLKGTFRYYKPEVGEMIKKRMAEIIEGVAKTHGARAEFSINDLVPPTINDKAMADFARKVAEKYRLRHGDVAMS
MGAEDFAYYLQRVPGAFLALGIRNEGKGIVHPHHHPKFDVDEDVLHLGTAMEVALAFEFLKD

Specific function: Can release basic, acidic, aromatic, and, to a lesser extent, aliphatic amino acids [H]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=428, Percent_Identity=29.2056074766355, Blast_Score=121, Evalue=9e-29,
Organism=Escherichia coli, GI1787598, Length=296, Percent_Identity=27.3648648648649, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 41849; Mature: 41849

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIEEALKIKDEIIAWRRDFHMHPELKYEEERTSRIVEEHLREWGYKIKRVGTGIIGDI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEHHHCCCCCCCC
GEGEKTIALRADMDALPVQEENDVPYRSRIPGKMHACGHDAHTAMLLGAAKIIAEHADEL
CCCCEEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GGKVRLIFQPAEEGGNGALKMIEGGALDGVDAIFGFHVWMDLPSGIIGIRDGPFLAGAGF
CCEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCC
FEAKVIGKGGHGASPHEAIDPIPIAAETVLALQTIVSRNVNPIETGVVSVTAINGGTTFN
EEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEE
VIPEEVTLKGTFRYYKPEVGEMIKKRMAEIIEGVAKTHGARAEFSINDLVPPTINDKAMA
ECCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
DFARKVAEKYRLRHGDVAMSMGAEDFAYYLQRVPGAFLALGIRNEGKGIVHPHHHPKFDV
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCC
DEDVLHLGTAMEVALAFEFLKD
CHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNPIEEALKIKDEIIAWRRDFHMHPELKYEEERTSRIVEEHLREWGYKIKRVGTGIIGDI
CCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCEEHHHCCCCCCCC
GEGEKTIALRADMDALPVQEENDVPYRSRIPGKMHACGHDAHTAMLLGAAKIIAEHADEL
CCCCEEEEEEECCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHC
GGKVRLIFQPAEEGGNGALKMIEGGALDGVDAIFGFHVWMDLPSGIIGIRDGPFLAGAGF
CCEEEEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCEEEECCCCEEECCCC
FEAKVIGKGGHGASPHEAIDPIPIAAETVLALQTIVSRNVNPIETGVVSVTAINGGTTFN
EEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEEEECCCCEEE
VIPEEVTLKGTFRYYKPEVGEMIKKRMAEIIEGVAKTHGARAEFSINDLVPPTINDKAMA
ECCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCHHHHH
DFARKVAEKYRLRHGDVAMSMGAEDFAYYLQRVPGAFLALGIRNEGKGIVHPHHHPKFDV
HHHHHHHHHHHHCCCCEEECCCHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCC
DEDVLHLGTAMEVALAFEFLKD
CHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7559343; 11427726; 1597179 [H]