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The map label for this gene is trpB

Identifier: 212223293

GI number: 212223293

Start: 121958

End: 123289

Strand: Reverse

Name: trpB

Synonym: TON_0147

Alternate gene names: 212223293

Gene position: 123289-121958 (Counterclockwise)

Preceding gene: 212223295

Following gene: 212223289

Centisome position: 6.67

GC content: 56.01

Gene sequence:

>1332_bases
ATGAAAGCCGTTCTGCCAGATTCCAAAATGCCAAAGAGGTGGTACAACATCCTGCCAGACCTACCGGAGCCTTTGGCACC
GCCCCTCGACCCGGAGACGGACGAGCCCATGGAGTCAGAGAAGCTGCTCCGCATCTTCGCATCTGAGTTGGTGAAGCAGG
AAATGAGCATGGAGCGATACATCGATATTCCAAAGAAAGTTCGTGAGCTCTACGCTAAAATAGGCCGTCCAACGCCCCTC
TTCAGGGCGACGAATCTCGAAAAGGCCCTCGAAACGCCGGCGAGGATATACTTCAAATACGAAGGGGCTACCGTAACGGG
CAGCCACAAGATAAACACCGCATTGGCCCAGGCATACTACGCGAAGAAGCAGGGCATCGAGAGGCTCGTAACAGAGACAG
GTGCAGGCCAGTGGGGAACTGCCCTGTCTCTGGCAGGGGCACTAATGGGGATAAACGTTCGTGTTTACATGGCTAGGGCC
AGCTTTTACCAGAAGCCCTACAGGAAGACTATAATGCGTCTCTACGGTGCCGAAATCTATCCCAGTCCAAGCGACAGGAC
CGAGATAGGAAGGAAGTTCCTGAGCGAAGACCCAAACCACCCAGGTGGCTTGGGAATAGCGATAAGCGAAGCTATCGAGG
ACGTTCTAAGGGACGAAAAAGCGCGCTACGCCCTCGGAAGCGTGCTCAACCACGTTTTAATGCACCAGACGGTCATAGGC
CTAGAAGCCAAAGAGCAGATGAAGGAGTTTGAGGATCCAGATGTCATAATTGGCTGCGTTGGGGGAGGAAGCAACTTCGC
AGGCCTGGCATATCCCTTCGTCAAGGATAGACTAGACGGCAAAGCTGACTACGAGTTCATAGCGGTTGAGCCAAGGGCGG
CACCGAGCATGACGCGCGGCGTTTACACCTACGACTACGGCGACTCTGGAGGGCTAACGCCGAAGATGAAGATGCACACC
CTCGGCCACACCTACTACGTCCCGCCGATACACGCTGGCGGCCTGAGGTACCACGGCCTGGCTCCAACGCTCAGTATTCT
GATAAACCACGGGATAGTTAGGCCAGTAGCCTACCATCAGACGGAGGTCTTCGAGGCGGCCCGGCTCTTTGCCAAGACGG
AAGGCATAGTCCCAGCACCCGAGAGCGCCCACGCGGTCAAGGCTGCAATAGACAGGGCCCTCAAGGCGAAGGAAGAAGGT
AAGGAAGAAGTCATCCTCTTCAACCTCAGCGGCCACGGCCTGCTCGACCTCAAGGGCTACGAGGATTTCCTCGACGGAAA
GCTCGAGGACTACGAGCCAGAAGAGCTTCCAGCTTTGAGGGGGGAGGTTTAG

Upstream 100 bases:

>100_bases
TATACTCCTACCCAGCTGGAACTTTCTGCTCTGGGTTAGTCCTCACGCTCATTATTCCCTCACCTTTGGGACTTCTTCCA
AAAATCAGGAGGTGGTTTGA

Downstream 100 bases:

>100_bases
CTTTTCCTCCCCAAAATCATCCTTGGATAGAAGTTCAAGCTTGCCTTGGCCTGGGGAATTTCCATGCCAAGCGCTATTCC
GAGGCTCATTAAGTCCCTCG

Product: tryptophan synthase subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 443; Mature: 443

Protein sequence:

>443_residues
MKAVLPDSKMPKRWYNILPDLPEPLAPPLDPETDEPMESEKLLRIFASELVKQEMSMERYIDIPKKVRELYAKIGRPTPL
FRATNLEKALETPARIYFKYEGATVTGSHKINTALAQAYYAKKQGIERLVTETGAGQWGTALSLAGALMGINVRVYMARA
SFYQKPYRKTIMRLYGAEIYPSPSDRTEIGRKFLSEDPNHPGGLGIAISEAIEDVLRDEKARYALGSVLNHVLMHQTVIG
LEAKEQMKEFEDPDVIIGCVGGGSNFAGLAYPFVKDRLDGKADYEFIAVEPRAAPSMTRGVYTYDYGDSGGLTPKMKMHT
LGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVRPVAYHQTEVFEAARLFAKTEGIVPAPESAHAVKAAIDRALKAKEEG
KEEVILFNLSGHGLLDLKGYEDFLDGKLEDYEPEELPALRGEV

Sequences:

>Translated_443_residues
MKAVLPDSKMPKRWYNILPDLPEPLAPPLDPETDEPMESEKLLRIFASELVKQEMSMERYIDIPKKVRELYAKIGRPTPL
FRATNLEKALETPARIYFKYEGATVTGSHKINTALAQAYYAKKQGIERLVTETGAGQWGTALSLAGALMGINVRVYMARA
SFYQKPYRKTIMRLYGAEIYPSPSDRTEIGRKFLSEDPNHPGGLGIAISEAIEDVLRDEKARYALGSVLNHVLMHQTVIG
LEAKEQMKEFEDPDVIIGCVGGGSNFAGLAYPFVKDRLDGKADYEFIAVEPRAAPSMTRGVYTYDYGDSGGLTPKMKMHT
LGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVRPVAYHQTEVFEAARLFAKTEGIVPAPESAHAVKAAIDRALKAKEEG
KEEVILFNLSGHGLLDLKGYEDFLDGKLEDYEPEELPALRGEV
>Mature_443_residues
MKAVLPDSKMPKRWYNILPDLPEPLAPPLDPETDEPMESEKLLRIFASELVKQEMSMERYIDIPKKVRELYAKIGRPTPL
FRATNLEKALETPARIYFKYEGATVTGSHKINTALAQAYYAKKQGIERLVTETGAGQWGTALSLAGALMGINVRVYMARA
SFYQKPYRKTIMRLYGAEIYPSPSDRTEIGRKFLSEDPNHPGGLGIAISEAIEDVLRDEKARYALGSVLNHVLMHQTVIG
LEAKEQMKEFEDPDVIIGCVGGGSNFAGLAYPFVKDRLDGKADYEFIAVEPRAAPSMTRGVYTYDYGDSGGLTPKMKMHT
LGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVRPVAYHQTEVFEAARLFAKTEGIVPAPESAHAVKAAIDRALKAKEEG
KEEVILFNLSGHGLLDLKGYEDFLDGKLEDYEPEELPALRGEV

Specific function: The beta subunit is responsible for the synthesis of L- tryptophan from indole and L-serine

COG id: COG1350

COG function: function code R; Predicted alternative tryptophan synthase beta-subunit (paralog of TrpB)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpB family

Homologues:

Organism=Escherichia coli, GI1787515, Length=382, Percent_Identity=30.8900523560209, Blast_Score=134, Evalue=2e-32,
Organism=Saccharomyces cerevisiae, GI6321412, Length=378, Percent_Identity=32.010582010582, Blast_Score=144, Evalue=3e-35,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRPB_THEON (B6YSU5)

Other databases:

- EMBL:   CP000855
- RefSeq:   YP_002306529.1
- GeneID:   7017801
- GenomeReviews:   CP000855_GR
- KEGG:   ton:TON_0147
- HOGENOM:   HBG303148
- OMA:   YTLGHDF
- ProtClustDB:   PRK12391
- HAMAP:   MF_00133
- InterPro:   IPR001926
- InterPro:   IPR006316
- InterPro:   IPR006653
- InterPro:   IPR023026
- TIGRFAMs:   TIGR01415

Pfam domain/function: PF00291 PALP; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.2.1.20

Molecular weight: Translated: 49199; Mature: 49199

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: PS00168 TRP_SYNTHASE_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAVLPDSKMPKRWYNILPDLPEPLAPPLDPETDEPMESEKLLRIFASELVKQEMSMERY
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IDIPKKVRELYAKIGRPTPLFRATNLEKALETPARIYFKYEGATVTGSHKINTALAQAYY
HCCHHHHHHHHHHHCCCCCCHHHCCHHHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHH
AKKQGIERLVTETGAGQWGTALSLAGALMGINVRVYMARASFYQKPYRKTIMRLYGAEIY
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHCCCCC
PSPSDRTEIGRKFLSEDPNHPGGLGIAISEAIEDVLRDEKARYALGSVLNHVLMHQTVIG
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LEAKEQMKEFEDPDVIIGCVGGGSNFAGLAYPFVKDRLDGKADYEFIAVEPRAAPSMTRG
CCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCC
VYTYDYGDSGGLTPKMKMHTLGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVRPVAYHQ
EEEEECCCCCCCCCCEEHEECCCEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCHHHHH
TEVFEAARLFAKTEGIVPAPESAHAVKAAIDRALKAKEEGKEEVILFNLSGHGLLDLKGY
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCC
EDFLDGKLEDYEPEELPALRGEV
HHHHCCCCCCCCCHHCCCCCCCC
>Mature Secondary Structure
MKAVLPDSKMPKRWYNILPDLPEPLAPPLDPETDEPMESEKLLRIFASELVKQEMSMERY
CCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
IDIPKKVRELYAKIGRPTPLFRATNLEKALETPARIYFKYEGATVTGSHKINTALAQAYY
HCCHHHHHHHHHHHCCCCCCHHHCCHHHHHCCCEEEEEEECCEEEECCCHHHHHHHHHHH
AKKQGIERLVTETGAGQWGTALSLAGALMGINVRVYMARASFYQKPYRKTIMRLYGAEIY
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCEEEEEEEHHHHHHHHHHHHHHHHHCCCCC
PSPSDRTEIGRKFLSEDPNHPGGLGIAISEAIEDVLRDEKARYALGSVLNHVLMHQTVIG
CCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
LEAKEQMKEFEDPDVIIGCVGGGSNFAGLAYPFVKDRLDGKADYEFIAVEPRAAPSMTRG
CCHHHHHHHCCCCCEEEEEECCCCCCCCHHHHHHHHHCCCCCCCEEEEECCCCCCHHHCC
VYTYDYGDSGGLTPKMKMHTLGHTYYVPPIHAGGLRYHGLAPTLSILINHGIVRPVAYHQ
EEEEECCCCCCCCCCEEHEECCCEEECCCCCCCCEEECCCHHHHHHHHHCCCCCCHHHHH
TEVFEAARLFAKTEGIVPAPESAHAVKAAIDRALKAKEEGKEEVILFNLSGHGLLDLKGY
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEECCCCEEEECCC
EDFLDGKLEDYEPEELPALRGEV
HHHHCCCCCCCCCHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA