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The map label for this gene is ubiE [H]

Identifier: 209696390

GI number: 209696390

Start: 3246486

End: 3247250

Strand: Direct

Name: ubiE [H]

Synonym: VSAL_I2996

Alternate gene names: 209696390

Gene position: 3246486-3247250 (Clockwise)

Preceding gene: 209696389

Following gene: 209696391

Centisome position: 97.63

GC content: 41.83

Gene sequence:

>765_bases
ATGACGGACAAAACGCACTCTACCGAAGAGACCACACACTTCGGTTTTACAACAGTAGCAAAAGAAGAAAAAGTAGCGAA
AGTTGCTGAAGTATTTCACTCTGTAGCGGCAAAATACGACATCATGAATGACCTTATGTCTGGTGGTGTTCATCGTATTT
GGAAGCGCTTTACTATTGATTGCAGTGGCGTACGTCCTGGACATAAAGTGCTTGATTTAGCCGGTGGTACAGGCGACTTA
ACGGCTAAGTTTTCTCGTATTGTTGGTGAGAAAGGCCAAGTTATTTTGGCTGATATTAACAACTCAATGTTGAATATTGG
TCGTGACAAATTAAGAGACATGGGCATTGTTGGTAACGTGAACTACGTGCAAGCAAATGCTGAAGAACTGCCTTTTCCTG
ATAACCATTTTGATTGTATTACAATCAGTTTTGGTCTGCGTAACGTGACAGACAAAGACAAAGCATTACGCTCGATGTTT
CGCGTGTTAAAGCCGGGCGGTCGCCTGTTGGTTCTTGAGTTCTCAAAACCTATCCTTGAACCATTATCAAAAATCTACGA
TGCTTATTCATTCCACCTGTTGCCTAAAATCGGGGAATTGATAGCCAACGATGCTGACAGTTATCGTTATCTTGCTGAAT
CTATTCGTATGCACCCAGATCAAGAAACACTGAAAGGCATGATGGAAGAGGCTGGCTTTGAGCAAGCAAACTACTACAAC
TTAACTGGTGGTATTGTTGCTCTGCATCGTGGTTACAAATTTTAA

Upstream 100 bases:

>100_bases
TGCAAACGCTTGGTGATAGTGGGTCGGATAAAGTAGACTAGAGCGCTGTTGATCCCACAGGATAAGAAGGGGTCAACATA
TTATCTCTAGAGGACTTAAA

Downstream 100 bases:

>100_bases
TAGGATAAAATGATGCCATTAGATCCGTTTGTAACAGGTGTGATTGAAACGGGGTTAAACGCCCTGCTAAAAGAATCGCC
AGAGAGCAAAGCTGCATTGC

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: S-Adenosyl-L-Homocysteine; @OCTA02.txt*2- Polyprenyl-3-Methyl-6-Methoxy-1,4-Benzoquinol!; Menaquinol. [C]

Alternate protein names: NA

Number of amino acids: Translated: 254; Mature: 253

Protein sequence:

>254_residues
MTDKTHSTEETTHFGFTTVAKEEKVAKVAEVFHSVAAKYDIMNDLMSGGVHRIWKRFTIDCSGVRPGHKVLDLAGGTGDL
TAKFSRIVGEKGQVILADINNSMLNIGRDKLRDMGIVGNVNYVQANAEELPFPDNHFDCITISFGLRNVTDKDKALRSMF
RVLKPGGRLLVLEFSKPILEPLSKIYDAYSFHLLPKIGELIANDADSYRYLAESIRMHPDQETLKGMMEEAGFEQANYYN
LTGGIVALHRGYKF

Sequences:

>Translated_254_residues
MTDKTHSTEETTHFGFTTVAKEEKVAKVAEVFHSVAAKYDIMNDLMSGGVHRIWKRFTIDCSGVRPGHKVLDLAGGTGDL
TAKFSRIVGEKGQVILADINNSMLNIGRDKLRDMGIVGNVNYVQANAEELPFPDNHFDCITISFGLRNVTDKDKALRSMF
RVLKPGGRLLVLEFSKPILEPLSKIYDAYSFHLLPKIGELIANDADSYRYLAESIRMHPDQETLKGMMEEAGFEQANYYN
LTGGIVALHRGYKF
>Mature_253_residues
TDKTHSTEETTHFGFTTVAKEEKVAKVAEVFHSVAAKYDIMNDLMSGGVHRIWKRFTIDCSGVRPGHKVLDLAGGTGDLT
AKFSRIVGEKGQVILADINNSMLNIGRDKLRDMGIVGNVNYVQANAEELPFPDNHFDCITISFGLRNVTDKDKALRSMFR
VLKPGGRLLVLEFSKPILEPLSKIYDAYSFHLLPKIGELIANDADSYRYLAESIRMHPDQETLKGMMEEAGFEQANYYNL
TGGIVALHRGYKF

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2) [H]

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family [H]

Homologues:

Organism=Homo sapiens, GI116063536, Length=276, Percent_Identity=45.2898550724638, Blast_Score=241, Evalue=4e-64,
Organism=Escherichia coli, GI48994983, Length=254, Percent_Identity=79.9212598425197, Blast_Score=438, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17557039, Length=254, Percent_Identity=45.6692913385827, Blast_Score=223, Evalue=9e-59,
Organism=Saccharomyces cerevisiae, GI6323526, Length=260, Percent_Identity=42.6923076923077, Blast_Score=181, Evalue=1e-46,
Organism=Drosophila melanogaster, GI24641702, Length=259, Percent_Identity=45.5598455598456, Blast_Score=246, Evalue=1e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004033 [H]

Pfam domain/function: PF01209 Ubie_methyltran [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 28407; Mature: 28276

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: PS01183 UBIE_1 ; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDKTHSTEETTHFGFTTVAKEEKVAKVAEVFHSVAAKYDIMNDLMSGGVHRIWKRFTID
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEE
CSGVRPGHKVLDLAGGTGDLTAKFSRIVGEKGQVILADINNSMLNIGRDKLRDMGIVGNV
ECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCHHHHHHCCCCCCC
NYVQANAEELPFPDNHFDCITISFGLRNVTDKDKALRSMFRVLKPGGRLLVLEFSKPILE
EEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
PLSKIYDAYSFHLLPKIGELIANDADSYRYLAESIRMHPDQETLKGMMEEAGFEQANYYN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEE
LTGGIVALHRGYKF
CCCHHHEECCCCCC
>Mature Secondary Structure 
TDKTHSTEETTHFGFTTVAKEEKVAKVAEVFHSVAAKYDIMNDLMSGGVHRIWKRFTID
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHEEE
CSGVRPGHKVLDLAGGTGDLTAKFSRIVGEKGQVILADINNSMLNIGRDKLRDMGIVGNV
ECCCCCCCCEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCHHHHHCHHHHHHCCCCCCC
NYVQANAEELPFPDNHFDCITISFGLRNVTDKDKALRSMFRVLKPGGRLLVLEFSKPILE
EEEECCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEECCCHHHH
PLSKIYDAYSFHLLPKIGELIANDADSYRYLAESIRMHPDQETLKGMMEEAGFEQANYYN
HHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCEEE
LTGGIVALHRGYKF
CCCHHHEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-Methionine; 2-Polyprenyl-6- Methoxy-1,4-Benzoquinol; Demethylmenaquinol [C]

Specific reaction: S-Adenosyl-L-Methionine + @OCTA02.txt*2-Polyprenyl-6- Methoxy-1,4-Benzoquinol! = S-Adenosyl-L-Homocysteine + 2- Polyprenyl-3-Methyl-6-Methoxy -1,4-Benzoquinol S-Adenosyl-L-Methionine + Demethylmenaquinol = S-Adenosyl-L-Homocysteine + Menaquinol. [C]

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA