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The map label for this gene is thiC

Identifier: 209696377

GI number: 209696377

Start: 3230776

End: 3232722

Strand: Direct

Name: thiC

Synonym: VSAL_I2983

Alternate gene names: 209696377

Gene position: 3230776-3232722 (Clockwise)

Preceding gene: 209696376

Following gene: 209696378

Centisome position: 97.16

GC content: 43.04

Gene sequence:

>1947_bases
ATGTCGTCAAGTCGTAAACAAGCGCGTTTGGATGCTAAATCAAACATTGAGTCGCTTTCTGTTCAACCTTATCCCAATTC
AAAAAAAATCTATATCGACGGTTCTCGTCCTGATATTCGTGTTCCAATGCGTGAAATTTCATTGGCTGATAGCTTAGTCG
GTGGAACAAAAGAAAACCCAACGCTGATACCAAACGAACCCATACAAGTCTATGACACCTCAGGGGTCTATACCGACCCA
AATTACGACATTGATGTTTACCAAGGGTTGCCTAAATTACGCGAACGTTGGATAGAAGACCGCACTGATACTGAAATGTT
GAAAGGGGTAAGCTCTGTTTATTCTCAAGAGCGCTTATCTGATGAAACGCTTGATGAGCTTCGTTATGGCAATCTACCAA
CAATTCGTCGAGCGAAAAAAGGACAATGTGTTACCCAACTTCATTATGCTCGCCAAGGTATTATTACCCCAGAAATGGAA
TACATCGCGATCCGTGAAAACATGGGACGTCAAAAATTTGCAGATGAACAACTAAATCATCAGCATCCTGGACACAGTTT
TGGTGCTAACCTTCCAAAAGAAATTACCCCTGAATTTGTACGTAACGAAGTCGCCGAAGGGCGAGCCATTATTCCTGCCA
ATATTAATCATCCAGAAGCAGAGCCAATGATCATTGGTCGTAATTTTTTAATTAAAGTTAATGCCAATATTGGTAATTCT
TCGGTCAGTTCGTCGATTGAAGAAGAAGTAGAGAAACTGGTTTGGTCTACGCGTTGGGGCGGCGATACGGTGATGGACTT
ATCGACGGGTCGTAATATTCACGAAACACGTGAATGGATCCTTCGTAATAGTCCAGTACCAATCGGCACGGTTCCGATGT
ACCAAGCACTAGAGAAAGTGAATGGCGTGGCTGAGAATCTAAACTGGGAAGTAATGCGAGATACCTTAATTGAGCAAGCA
GAGCAGGGTGTAGATTACTTTACGATTCATGCCGGTTTACTGCTTCGTTATGTACCAATGACGGCGAAACGAGTGACAGG
GATTGTTTCTCGTGGCGGTTCTATCATTGCAAAATGGTGTTTAGCGCATCATCAAGAAAGTTTCTTATATACGCATTTTC
GTGAAATTTGTGAGATTTGTGCAAAGTACGATGTTTCTCTTTCTTTAGGGGATGGTTTACGTCCTGGCTCAATTGCGGAT
GCGAATGATGAAGCACAGTTTGCTGAATTGAGAACGCTCGGTGAGTTAACTAAAGTTGCTTGGGAATACGATGTTCAAGT
GATTATTGAGGGGCCGGGTCATGTACCAATGCATATGATCAAAGAGAACATGGACGAGCAACTCAAGCATTGTCATGAAG
CACCGTTCTATACATTAGGTCCATTAACGACTGATATTGCCCCTGGTTATGATCACATTACTTCGGGGATTGGTGCGGCC
ATGATTGGATGGTATGGCTGTGCAATGCTTTGTTATGTCACACCGAAAGAACATTTAGGCCTACCAAATAAAGAAGACGT
TAAGATTGGTTTGATCACCTATAAATTAGCCGCTCATGCGGGTGATTTAGCCAAAGGTCATCCGGGTGCCCAAATTCGAG
ATAACGCCTTATCAAAAGCCCGTTTTGAATTTCGTTGGGAAGACCAGTTTAATCTGTCACTTGATCCGATTACCGCTCGT
GAATTTCATGATGAAACACTGCCTCAAGAGTCGGGCAAGGTTGCTCACTTTTGTTCAATGTGCGGGCCTAAATTCTGCTC
AATGAAAATATCACAAGAAGTTCGAGAGTATGCCAAAGACACAGAACAAGTGGCGTTAGACCAAGCGATTAATATCAAAA
TGCTCGATGACCCACTTGAAGGTATGCGCCAAAAATCAGCAGAGTTTAAGGCCTCTGGTTCTGAGTTGTACCATCCTGCT
GTAGATGCAGAACCCGTGAAAAGCTAA

Upstream 100 bases:

>100_bases
TTAAGTAGACGATTTTTCTACTTTATATTTACGCACACTAAGAACCTCTTGCTACATAATCCGACAAGCAATTATCCTAA
TTATTACAAGGAATTTTGCT

Downstream 100 bases:

>100_bases
CGACAGTTACCATTATTATTTTAATCATGAATGGTGGTGTTTCACGTGGAACACCACTCTGGAGATCAAACGATGTATTC
TTTTCCTGCAATCGATAGAG

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase; HMP-P synthase; HMP-phosphate synthase; HMPP synthase; Thiamine biosynthesis protein thiC

Number of amino acids: Translated: 648; Mature: 647

Protein sequence:

>648_residues
MSSSRKQARLDAKSNIESLSVQPYPNSKKIYIDGSRPDIRVPMREISLADSLVGGTKENPTLIPNEPIQVYDTSGVYTDP
NYDIDVYQGLPKLRERWIEDRTDTEMLKGVSSVYSQERLSDETLDELRYGNLPTIRRAKKGQCVTQLHYARQGIITPEME
YIAIRENMGRQKFADEQLNHQHPGHSFGANLPKEITPEFVRNEVAEGRAIIPANINHPEAEPMIIGRNFLIKVNANIGNS
SVSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLNWEVMRDTLIEQA
EQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVSLSLGDGLRPGSIAD
ANDEAQFAELRTLGELTKVAWEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAA
MIGWYGCAMLCYVTPKEHLGLPNKEDVKIGLITYKLAAHAGDLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPITAR
EFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDTEQVALDQAINIKMLDDPLEGMRQKSAEFKASGSELYHPA
VDAEPVKS

Sequences:

>Translated_648_residues
MSSSRKQARLDAKSNIESLSVQPYPNSKKIYIDGSRPDIRVPMREISLADSLVGGTKENPTLIPNEPIQVYDTSGVYTDP
NYDIDVYQGLPKLRERWIEDRTDTEMLKGVSSVYSQERLSDETLDELRYGNLPTIRRAKKGQCVTQLHYARQGIITPEME
YIAIRENMGRQKFADEQLNHQHPGHSFGANLPKEITPEFVRNEVAEGRAIIPANINHPEAEPMIIGRNFLIKVNANIGNS
SVSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLNWEVMRDTLIEQA
EQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVSLSLGDGLRPGSIAD
ANDEAQFAELRTLGELTKVAWEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAA
MIGWYGCAMLCYVTPKEHLGLPNKEDVKIGLITYKLAAHAGDLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPITAR
EFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDTEQVALDQAINIKMLDDPLEGMRQKSAEFKASGSELYHPA
VDAEPVKS
>Mature_647_residues
SSSRKQARLDAKSNIESLSVQPYPNSKKIYIDGSRPDIRVPMREISLADSLVGGTKENPTLIPNEPIQVYDTSGVYTDPN
YDIDVYQGLPKLRERWIEDRTDTEMLKGVSSVYSQERLSDETLDELRYGNLPTIRRAKKGQCVTQLHYARQGIITPEMEY
IAIRENMGRQKFADEQLNHQHPGHSFGANLPKEITPEFVRNEVAEGRAIIPANINHPEAEPMIIGRNFLIKVNANIGNSS
VSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKVNGVAENLNWEVMRDTLIEQAE
QGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWCLAHHQESFLYTHFREICEICAKYDVSLSLGDGLRPGSIADA
NDEAQFAELRTLGELTKVAWEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAAM
IGWYGCAMLCYVTPKEHLGLPNKEDVKIGLITYKLAAHAGDLAKGHPGAQIRDNALSKARFEFRWEDQFNLSLDPITARE
FHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKDTEQVALDQAINIKMLDDPLEGMRQKSAEFKASGSELYHPAV
DAEPVKS

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family

Homologues:

Organism=Escherichia coli, GI1790427, Length=634, Percent_Identity=75.7097791798107, Blast_Score=1010, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIC_ALISL (B6EGV1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264308.1
- GeneID:   6988200
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2983
- HOGENOM:   HBG285234
- OMA:   TWELFRD
- ProtClustDB:   PRK09284
- HAMAP:   MF_00089
- InterPro:   IPR002817
- TIGRFAMs:   TIGR00190

Pfam domain/function: PF01964 ThiC

EC number: NA

Molecular weight: Translated: 72713; Mature: 72582

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: NA

Important sites: BINDING 236-236 BINDING 265-265 BINDING 294-294 BINDING 330-330 BINDING 430-430 BINDING 457-457

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSRKQARLDAKSNIESLSVQPYPNSKKIYIDGSRPDIRVPMREISLADSLVGGTKENP
CCCCHHHHHCCHHCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCC
TLIPNEPIQVYDTSGVYTDPNYDIDVYQGLPKLRERWIEDRTDTEMLKGVSSVYSQERLS
EECCCCCEEEEECCCEEECCCCCCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
DETLDELRYGNLPTIRRAKKGQCVTQLHYARQGIITPEMEYIAIRENMGRQKFADEQLNH
HHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHHHCC
QHPGHSFGANLPKEITPEFVRNEVAEGRAIIPANINHPEAEPMIIGRNFLIKVNANIGNS
CCCCCCCCCCCCHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCH
SVSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKV
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
NGVAENLNWEVMRDTLIEQAEQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWC
HHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LAHHQESFLYTHFREICEICAKYDVSLSLGDGLRPGSIADANDEAQFAELRTLGELTKVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAA
HEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
MIGWYGCAMLCYVTPKEHLGLPNKEDVKIGLITYKLAAHAGDLAKGHPGAQIRDNALSKA
HHHHHHHHHHEEECCHHHCCCCCCCCEEEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHC
RFEFRWEDQFNLSLDPITAREFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKD
EEEEEECCCCCCEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
TEQVALDQAINIKMLDDPLEGMRQKSAEFKASGSELYHPAVDAEPVKS
HHHHHHHHHCEEEECCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC
>Mature Secondary Structure 
SSSRKQARLDAKSNIESLSVQPYPNSKKIYIDGSRPDIRVPMREISLADSLVGGTKENP
CCCHHHHHCCHHCCCCCEEECCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCC
TLIPNEPIQVYDTSGVYTDPNYDIDVYQGLPKLRERWIEDRTDTEMLKGVSSVYSQERLS
EECCCCCEEEEECCCEEECCCCCCHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHCC
DETLDELRYGNLPTIRRAKKGQCVTQLHYARQGIITPEMEYIAIRENMGRQKFADEQLNH
HHHHHHHHCCCCCHHHHCCCCCHHHHHHHHHCCCCCCCHHEEEEHHHCCHHHHHHHHHCC
QHPGHSFGANLPKEITPEFVRNEVAEGRAIIPANINHPEAEPMIIGRNFLIKVNANIGNS
CCCCCCCCCCCCHHCCHHHHHHHHHCCCEEEECCCCCCCCCCEEECCEEEEEEECCCCCH
SVSSSIEEEVEKLVWSTRWGGDTVMDLSTGRNIHETREWILRNSPVPIGTVPMYQALEKV
HHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHH
NGVAENLNWEVMRDTLIEQAEQGVDYFTIHAGLLLRYVPMTAKRVTGIVSRGGSIIAKWC
HHHHHCCCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LAHHQESFLYTHFREICEICAKYDVSLSLGDGLRPGSIADANDEAQFAELRTLGELTKVA
HHHHHHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
WEYDVQVIIEGPGHVPMHMIKENMDEQLKHCHEAPFYTLGPLTTDIAPGYDHITSGIGAA
HEECEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHH
MIGWYGCAMLCYVTPKEHLGLPNKEDVKIGLITYKLAAHAGDLAKGHPGAQIRDNALSKA
HHHHHHHHHHEEECCHHHCCCCCCCCEEEEEEEEEHHHCCCCCCCCCCCCCHHHHHHHHC
RFEFRWEDQFNLSLDPITAREFHDETLPQESGKVAHFCSMCGPKFCSMKISQEVREYAKD
EEEEEECCCCCCEECCCCHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHH
TEQVALDQAINIKMLDDPLEGMRQKSAEFKASGSELYHPAVDAEPVKS
HHHHHHHHHCEEEECCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA