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The map label for this gene is rpoH [H]

Identifier: 209696299

GI number: 209696299

Start: 3145988

End: 3146848

Strand: Reverse

Name: rpoH [H]

Synonym: VSAL_I2900

Alternate gene names: 209696299

Gene position: 3146848-3145988 (Counterclockwise)

Preceding gene: 209696300

Following gene: 209696298

Centisome position: 94.64

GC content: 40.19

Gene sequence:

>861_bases
ATGTCGAAAGAGATGTATTCAATGGCTGTCGTTTCTCAAGATAGCATCGATAGCTATCTACGTTCTGTTAATCGTTACCC
GATGTTAACGGTTGAGAAAGAACGCGATCTTGCTGAACGTCTACACTACAAAGAAGAAATAGAGGCGGCAAAAGAGCTGA
TTTTATCTCATCTACGCTTCGTTGTTCATGTCGCTCGTGGATATTCTGGTTATGGCTTACCTTTGGCGGATCTTATCCAA
GAAGGTAATGTTGGTTTGATGAAAGCAGTAAAACGATTTAACCCTGAAGTGGGTGTTCGTTTGGTTTCATTTGCAGTGCA
TTGGATTAAAGCTGAAATTCATGAATACGTATTAAGAAACTGGCGTATCGTGAAAGTAGCAACAACCAAAGCGCAGCGTA
AACTGTTCTTCAATCTTAGAAAGAATAAAAAACGTCTTGGTTGGTTTAATAATGGCGAAGTTGAAGCCGTTGCTAAAGAC
TTAGGTGTATCGCCTTCTGAAGTTAGAGAGATGGAATCCCGTCTTGCAGCTCAAGATTCAACGTTTGAAGCTCCAACCGA
AGATGACGACCGTGATATGGCGTTTACCGCTCCTGTTTATTACTTAGAAGATAAGCAGTCAGATATTGCTCATTCATTAG
AAGAAGATAATTGGGAAGATTACACCAATAATCGTTTGGCTAATGCGATAGCAACGTTAGATGACCGCAGTCAACATATT
GTTCAATCTCGTTGGTTAACTGATGAGAAGACGACGTTGCAAGAGTTAGCTAACACTTACTCTGTGTCTGCTGAGCGAAT
TCGTCAGCTTGAAAAGAACGCGATGAAAAAATTAAAAGATGCTGTAGGAGATATATTCTAA

Upstream 100 bases:

>100_bases
GTGTGGATAATTTTCCGCCTTTCCCTTGCAGTCTGTTTATTTTAAGTACATTATTGCAAGGTTAAGTTGAAGTTAAATCT
AGATATAAATGGGGATTTGA

Downstream 100 bases:

>100_bases
CTACCGCATTATCATTATACACAGAAGGCTGAATCCGAAAGGGTTCAGCCTTTTTTATTGGTTAAAATATTGTGATTAGG
ATCATAAGGTGTTTTTAAAT

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 286; Mature: 285

Protein sequence:

>286_residues
MSKEMYSMAVVSQDSIDSYLRSVNRYPMLTVEKERDLAERLHYKEEIEAAKELILSHLRFVVHVARGYSGYGLPLADLIQ
EGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKD
LGVSPSEVREMESRLAAQDSTFEAPTEDDDRDMAFTAPVYYLEDKQSDIAHSLEEDNWEDYTNNRLANAIATLDDRSQHI
VQSRWLTDEKTTLQELANTYSVSAERIRQLEKNAMKKLKDAVGDIF

Sequences:

>Translated_286_residues
MSKEMYSMAVVSQDSIDSYLRSVNRYPMLTVEKERDLAERLHYKEEIEAAKELILSHLRFVVHVARGYSGYGLPLADLIQ
EGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKD
LGVSPSEVREMESRLAAQDSTFEAPTEDDDRDMAFTAPVYYLEDKQSDIAHSLEEDNWEDYTNNRLANAIATLDDRSQHI
VQSRWLTDEKTTLQELANTYSVSAERIRQLEKNAMKKLKDAVGDIF
>Mature_285_residues
SKEMYSMAVVSQDSIDSYLRSVNRYPMLTVEKERDLAERLHYKEEIEAAKELILSHLRFVVHVARGYSGYGLPLADLIQE
GNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKDL
GVSPSEVREMESRLAAQDSTFEAPTEDDDRDMAFTAPVYYLEDKQSDIAHSLEEDNWEDYTNNRLANAIATLDDRSQHIV
QSRWLTDEKTTLQELANTYSVSAERIRQLEKNAMKKLKDAVGDIF

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=283, Percent_Identity=67.1378091872791, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1789448, Length=270, Percent_Identity=28.1481481481481, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI1789098, Length=272, Percent_Identity=25.7352941176471, Blast_Score=91, Evalue=9e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33007; Mature: 32876

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEMYSMAVVSQDSIDSYLRSVNRYPMLTVEKERDLAERLHYKEEIEAAKELILSHLRF
CCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVHVARGYSGYGLPLADLIQEGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKDLGVSPSEVREMESRLAAQDS
CEEEEEEHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TFEAPTEDDDRDMAFTAPVYYLEDKQSDIAHSLEEDNWEDYTNNRLANAIATLDDRSQHI
CCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHH
VQSRWLTDEKTTLQELANTYSVSAERIRQLEKNAMKKLKDAVGDIF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKEMYSMAVVSQDSIDSYLRSVNRYPMLTVEKERDLAERLHYKEEIEAAKELILSHLRF
CCHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VVHVARGYSGYGLPLADLIQEGNVGLMKAVKRFNPEVGVRLVSFAVHWIKAEIHEYVLRN
HHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHC
WRIVKVATTKAQRKLFFNLRKNKKRLGWFNNGEVEAVAKDLGVSPSEVREMESRLAAQDS
CEEEEEEHHHHHHHHHHHHHHCHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHCCC
TFEAPTEDDDRDMAFTAPVYYLEDKQSDIAHSLEEDNWEDYTNNRLANAIATLDDRSQHI
CCCCCCCCCCCCEEEECCEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCHHHHH
VQSRWLTDEKTTLQELANTYSVSAERIRQLEKNAMKKLKDAVGDIF
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA