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The map label for this gene is plsB

Identifier: 209696293

GI number: 209696293

Start: 3139863

End: 3142286

Strand: Direct

Name: plsB

Synonym: VSAL_I2894

Alternate gene names: 209696293

Gene position: 3139863-3142286 (Clockwise)

Preceding gene: 209696289

Following gene: 209696296

Centisome position: 94.43

GC content: 40.72

Gene sequence:

>2424_bases
ATGTCTACAGGACAAACTATTTATCACTCTTTACTCAAGTTGCCGTTATCGGTCATGGTAAAGAGCACTCCTATTCCATC
GAATCCAATTGAAGATCTCAATATCGATATTGAGCGCCCAATTATTTATGCACTGCCGTTTCGATCTCATGTCGATCTGC
TCACATTACAAAAAAGTGCTAAAGAGTTAGGGCTACCAGATCCTCTTTCTCCAATTGAAATTGATGGTGTGTCTTACCCT
CGTTATGTATTCACTTCGATCGGACCAAAGATGTTCGATACTGATGATGATTTACCACAAGAGTCTCTGGATTTATTTAA
AATTGTTCTCAAGCACCATGCAGATAATCCTGATGCTGATTTTCAATTAATCCCGACGTCAATTCTTTGGGGAAGAAGAC
CTGGAAAAGAAGGCACAAGTAGGCCTCATTTAATGCCATTAAATGGGCCACAAAAGTTTGTGACTCTAATTAAAGCTGGT
CGTGATTCAACGGTACGCATCAGTCCTGTCGTTTCGTTGCGCTACATGGCAGACAATCACGGTGCAGATGATGCAATAGC
TCATAAACTGGCTCGTGTAGCAAAGATCCACTTCTCACGTCAGAAATTAGCGGCATCAGGACCAAACCTTCCGAATCGCC
AAGCGTTATTCAATCGGTTATTAAAATCTCAAGCTATTGAGAAAGTGATTTTAGAAGAAGCGAGGATCCGTAACGTTGAT
GTAGAGAAAGTACGTAAAGAAGCCATGGGGATCATGGAAGAAATTGCAACGAACTTCTCTTATTCGCTGATCAAGAATGG
CAATCGAATTCTTAAATGGCTATGGAACCGTCTATACCAAGGCTTAAACATCAACAACGCCGCGACCGTGCGTAAATTAG
CACAAGAAGGCCATGAGATTGTTTATGTACCTTGTCACCGCAGCCACATGGATTACCTACTGCTTTCGTATGTTCTTTAC
CATGAGGGCTTAGTACCACCACATATTGCTGCTGGTATTAACTTAAACTTTTTCCCTGCAGGCCCTATATTCCGCCGTGG
CGGTGCTTTCTTTATTCGTCGTAGCTTTAAAGGTAACCGACTTTACTCTACTATTTTTCGTGAATATTTAGCCGAGTTGT
TTGCGAAAGGCTATTCCGTCGAATATTTCAGTGAAGGGGGTCGTTCTCGTACCGGTCGCCTATTGCAGGCCAAAACGGGC
ATGCTAGCCATGACAGTACAAGCGATGTTACGAGGCTTAAACCGCCCTGTAACCTTGGTGCCTGTGTATATTGGTTATGA
ACACGTAATGGAAGTAACGACTTACGCAAAAGAGTTACAAGGCAAACGTAAAGAAAAAGAAAACGCAGGACAAGTACTAC
GCACGCTTCGTAAACTGCGTAACTTTGGTCAAGGCTATGTAAACTTTGGCGAGCCGATTTCTTTAAACCATTACCTTAAT
GAACACGCACCTAATTGGTCTGAGTCCATCAACCCAATTGAACCACAAAAGCCAGAGTGGATGTCCCCTGTAGTTAATGG
CATCGCAAATAAAATGATGACACACATTAATGACGCAGTCGCAGCCAATGCATTAACACTATGTGCGACCGCTTTACTTG
CGGCTCGTCAGCGCGCTCTTAGTAAGGAAGATTTAACTGAGCAACTGGATTGCTACCTGCAGTTATTACGAAACATTCCT
TATTCGAATACGGCAACAGTACCCACTCAAGATGCCGAAGCGCTATTAGAGCACGCGATTGCATTAGACAAATTTGTTAT
TGAAAAAGATACGCTAGGTGAGATTATTTCATTAGACCGAAATCAATCAATATTAATGACCTATTACCGCAATAACATTA
TTCATCTTTTTGCATTACCGTCTTTAATAGCAAAATTAGTTGTGCAATACCGTTCAATTTCTATCGATAACGTTCAGGCA
CAAATTCAACAGATTTATCCTTTCCTTAAAGCCGAATTATTTCTGCATTATGATGAGAGTGAATTAAACGATGTGGTGAG
TCAGCATATCGACGAATTAGTTCGTCAAAAGCTGATCGAAAGAGAAAATGATGTTTTACAACTCAATGCCACCAATATTC
TGAAAGTGCATTTACTTGCTCATACGATTTCAGAAACACTGCAACGTTACGCTATTGCATTAACACACTTACAAGCGTCT
CCTAAGTTAGGTAAAAATGATTTAGAAGAGCAAAGTCAAATTATGGCGCAACGTTTAAGTCGTTTACATGGTATTAATGC
CCCGGAATTTTTCGATAAAGGTGTGTTTGGTATTCTATTTAATACTTTAAAAGCAGAAGGGTATTTAAACAGTGATGGCG
TTGCTGTTATAAGTAAAGTAGAGCCATTTTCTCGAGATATGTCTCGACTACTTAATCCTGAGATCAAGCTCACCATTCAA
GCAGTCATGACAAAAGAAGACTAA

Upstream 100 bases:

>100_bases
TGATTAAAAGGTTTGACCAGTTCTGCTATTCTTGCCAATAAAGTGTAAGCATCTATATATAACCGAGCATTCGGTACATA
ACTAAGGCGTTATTGTCATT

Downstream 100 bases:

>100_bases
ATCAGCTATCTCTTAATATTAAGATAATAAAAAACCAGTACACGAAACGTACTGGTTTTTTTATGCGTCTAATTTAAAGT
TTTGAAGTCATTAAATAAGT

Product: glycerol-3-phosphate acyltransferase

Products: NA

Alternate protein names: GPAT

Number of amino acids: Translated: 807; Mature: 806

Protein sequence:

>807_residues
MSTGQTIYHSLLKLPLSVMVKSTPIPSNPIEDLNIDIERPIIYALPFRSHVDLLTLQKSAKELGLPDPLSPIEIDGVSYP
RYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDADFQLIPTSILWGRRPGKEGTSRPHLMPLNGPQKFVTLIKAG
RDSTVRISPVVSLRYMADNHGADDAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEARIRNVD
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNAATVRKLAQEGHEIVYVPCHRSHMDYLLLSYVLY
HEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTG
MLAMTVQAMLRGLNRPVTLVPVYIGYEHVMEVTTYAKELQGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EHAPNWSESINPIEPQKPEWMSPVVNGIANKMMTHINDAVAANALTLCATALLAARQRALSKEDLTEQLDCYLQLLRNIP
YSNTATVPTQDAEALLEHAIALDKFVIEKDTLGEIISLDRNQSILMTYYRNNIIHLFALPSLIAKLVVQYRSISIDNVQA
QIQQIYPFLKAELFLHYDESELNDVVSQHIDELVRQKLIERENDVLQLNATNILKVHLLAHTISETLQRYAIALTHLQAS
PKLGKNDLEEQSQIMAQRLSRLHGINAPEFFDKGVFGILFNTLKAEGYLNSDGVAVISKVEPFSRDMSRLLNPEIKLTIQ
AVMTKED

Sequences:

>Translated_807_residues
MSTGQTIYHSLLKLPLSVMVKSTPIPSNPIEDLNIDIERPIIYALPFRSHVDLLTLQKSAKELGLPDPLSPIEIDGVSYP
RYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDADFQLIPTSILWGRRPGKEGTSRPHLMPLNGPQKFVTLIKAG
RDSTVRISPVVSLRYMADNHGADDAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEARIRNVD
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNAATVRKLAQEGHEIVYVPCHRSHMDYLLLSYVLY
HEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTG
MLAMTVQAMLRGLNRPVTLVPVYIGYEHVMEVTTYAKELQGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EHAPNWSESINPIEPQKPEWMSPVVNGIANKMMTHINDAVAANALTLCATALLAARQRALSKEDLTEQLDCYLQLLRNIP
YSNTATVPTQDAEALLEHAIALDKFVIEKDTLGEIISLDRNQSILMTYYRNNIIHLFALPSLIAKLVVQYRSISIDNVQA
QIQQIYPFLKAELFLHYDESELNDVVSQHIDELVRQKLIERENDVLQLNATNILKVHLLAHTISETLQRYAIALTHLQAS
PKLGKNDLEEQSQIMAQRLSRLHGINAPEFFDKGVFGILFNTLKAEGYLNSDGVAVISKVEPFSRDMSRLLNPEIKLTIQ
AVMTKED
>Mature_806_residues
STGQTIYHSLLKLPLSVMVKSTPIPSNPIEDLNIDIERPIIYALPFRSHVDLLTLQKSAKELGLPDPLSPIEIDGVSYPR
YVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDADFQLIPTSILWGRRPGKEGTSRPHLMPLNGPQKFVTLIKAGR
DSTVRISPVVSLRYMADNHGADDAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEARIRNVDV
EKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNAATVRKLAQEGHEIVYVPCHRSHMDYLLLSYVLYH
EGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNRLYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGM
LAMTVQAMLRGLNRPVTLVPVYIGYEHVMEVTTYAKELQGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLNE
HAPNWSESINPIEPQKPEWMSPVVNGIANKMMTHINDAVAANALTLCATALLAARQRALSKEDLTEQLDCYLQLLRNIPY
SNTATVPTQDAEALLEHAIALDKFVIEKDTLGEIISLDRNQSILMTYYRNNIIHLFALPSLIAKLVVQYRSISIDNVQAQ
IQQIYPFLKAELFLHYDESELNDVVSQHIDELVRQKLIERENDVLQLNATNILKVHLLAHTISETLQRYAIALTHLQASP
KLGKNDLEEQSQIMAQRLSRLHGINAPEFFDKGVFGILFNTLKAEGYLNSDGVAVISKVEPFSRDMSRLLNPEIKLTIQA
VMTKED

Specific function: De novo phospholipid biosynthesis; first step. [C]

COG id: COG2937

COG function: function code I; Glycerol-3-phosphate O-acyltransferase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GPAT/DAPAT family

Homologues:

Organism=Homo sapiens, GI7657134, Length=430, Percent_Identity=30.4651162790698, Blast_Score=166, Evalue=8e-41,
Organism=Homo sapiens, GI190358539, Length=429, Percent_Identity=24.2424242424242, Blast_Score=122, Evalue=2e-27,
Organism=Escherichia coli, GI87082362, Length=807, Percent_Identity=57.6208178438662, Blast_Score=946, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25147672, Length=267, Percent_Identity=35.2059925093633, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71988723, Length=490, Percent_Identity=27.3469387755102, Blast_Score=144, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71988728, Length=407, Percent_Identity=28.992628992629, Blast_Score=134, Evalue=2e-31,
Organism=Drosophila melanogaster, GI17864692, Length=274, Percent_Identity=33.5766423357664, Blast_Score=159, Evalue=8e-39,
Organism=Drosophila melanogaster, GI21357731, Length=327, Percent_Identity=28.4403669724771, Blast_Score=126, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24650754, Length=327, Percent_Identity=28.4403669724771, Blast_Score=126, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24650752, Length=327, Percent_Identity=28.4403669724771, Blast_Score=125, Evalue=9e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PLSB_ALISL (B6ENU1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264224.1
- GeneID:   6988749
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2894
- HOGENOM:   HBG296590
- OMA:   VIYVPCH
- ProtClustDB:   PRK04974
- HAMAP:   MF_00393
- InterPro:   IPR002123
- InterPro:   IPR022284
- SMART:   SM00563
- TIGRFAMs:   TIGR03703

Pfam domain/function: PF01553 Acyltransferase

EC number: =2.3.1.15

Molecular weight: Translated: 91260; Mature: 91128

Theoretical pI: Translated: 9.03; Mature: 9.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTGQTIYHSLLKLPLSVMVKSTPIPSNPIEDLNIDIERPIIYALPFRSHVDLLTLQKSA
CCCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEECCCEEEEECCCCCCHHHHHHHHH
KELGLPDPLSPIEIDGVSYPRYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDAD
HHCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FQLIPTSILWGRRPGKEGTSRPHLMPLNGPQKFVTLIKAGRDSTVRISPVVSLRYMADNH
EEEECHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
GADDAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEARIRNVD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNAATVRKLAQEGHEI
HHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGMLAMTVQAMLRGLNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
PVYIGYEHVMEVTTYAKELQGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
EHAPNWSESINPIEPQKPEWMSPVVNGIANKMMTHINDAVAANALTLCATALLAARQRAL
HCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKEDLTEQLDCYLQLLRNIPYSNTATVPTQDAEALLEHAIALDKFVIEKDTLGEIISLDR
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
NQSILMTYYRNNIIHLFALPSLIAKLVVQYRSISIDNVQAQIQQIYPFLKAELFLHYDES
CCCEEEEEHHCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECHH
ELNDVVSQHIDELVRQKLIERENDVLQLNATNILKVHLLAHTISETLQRYAIALTHLQAS
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKLGKNDLEEQSQIMAQRLSRLHGINAPEFFDKGVFGILFNTLKAEGYLNSDGVAVISKV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECC
EPFSRDMSRLLNPEIKLTIQAVMTKED
CHHHHHHHHHCCCCCEEEEEEEEECCC
>Mature Secondary Structure 
STGQTIYHSLLKLPLSVMVKSTPIPSNPIEDLNIDIERPIIYALPFRSHVDLLTLQKSA
CCCHHHHHHHHHCCHHHHHCCCCCCCCCCCCCCCEECCCEEEEECCCCCCHHHHHHHHH
KELGLPDPLSPIEIDGVSYPRYVFTSIGPKMFDTDDDLPQESLDLFKIVLKHHADNPDAD
HHCCCCCCCCCEEECCCCCCHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCC
FQLIPTSILWGRRPGKEGTSRPHLMPLNGPQKFVTLIKAGRDSTVRISPVVSLRYMADNH
EEEECHHHHHCCCCCCCCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEEEEEEECCCC
GADDAIAHKLARVAKIHFSRQKLAASGPNLPNRQALFNRLLKSQAIEKVILEEARIRNVD
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
VEKVRKEAMGIMEEIATNFSYSLIKNGNRILKWLWNRLYQGLNINNAATVRKLAQEGHEI
HHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCEE
VYVPCHRSHMDYLLLSYVLYHEGLVPPHIAAGINLNFFPAGPIFRRGGAFFIRRSFKGNR
EEECCCCCHHHHHHHHHHHHHCCCCCCHHHCCCCEEEECCCCHHHCCCEEEEEECCCCCH
LYSTIFREYLAELFAKGYSVEYFSEGGRSRTGRLLQAKTGMLAMTVQAMLRGLNRPVTLV
HHHHHHHHHHHHHHHCCCCEEHHHCCCCCCCCCHHHHHCCHHHHHHHHHHHHCCCCEEEE
PVYIGYEHVMEVTTYAKELQGKRKEKENAGQVLRTLRKLRNFGQGYVNFGEPISLNHYLN
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEHHHHHH
EHAPNWSESINPIEPQKPEWMSPVVNGIANKMMTHINDAVAANALTLCATALLAARQRAL
HCCCCHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
SKEDLTEQLDCYLQLLRNIPYSNTATVPTQDAEALLEHAIALDKFVIEKDTLGEIISLDR
CHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHHCCC
NQSILMTYYRNNIIHLFALPSLIAKLVVQYRSISIDNVQAQIQQIYPFLKAELFLHYDES
CCCEEEEEHHCCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEECHH
ELNDVVSQHIDELVRQKLIERENDVLQLNATNILKVHLLAHTISETLQRYAIALTHLQAS
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
PKLGKNDLEEQSQIMAQRLSRLHGINAPEFFDKGVFGILFNTLKAEGYLNSDGVAVISKV
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHHHHHHHHHHCCCCCCCCCEEEECC
EPFSRDMSRLLNPEIKLTIQAVMTKED
CHHHHHHHHHCCCCCEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA