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The map label for this gene is trmA

Identifier: 209696286

GI number: 209696286

Start: 3132667

End: 3133773

Strand: Direct

Name: trmA

Synonym: VSAL_I2887

Alternate gene names: 209696286

Gene position: 3132667-3133773 (Clockwise)

Preceding gene: 209696282

Following gene: 209696289

Centisome position: 94.21

GC content: 37.49

Gene sequence:

>1107_bases
ATGACACAACCCGTCATGAACCCAGAAAATTATCAAGTACAGTTAGATGAGAAAACAGAAGCACTGTCTGCTATGTTTTC
TGAATTTGACGTTCCAGAACTGGAAGTCTTTTCTTCTCCTGCTGAAAACTACCGTATGCGTGCAGAGTTTCGAATTTGGC
ACGAAGGTGACGATATGTATTACGTCATGTTCGATCAAAAAACAAAAGAAAAGTACCGTGTAGATTACTTCTTACCCGCC
ACTCGTCTTATTAACGACTTGATGCCACTACTTGCAGAAGCAATAAAAGGAAGTAAAACACTGCGTTATAAGATGTTCCA
AGTGGATTTTTTATCAACATTAAGCGGTGAAATCTTAGTTTCGATGCTTTATCACCGTCAATTAGACGATGCTTGGAAAG
AAGAAGCCGCAGTATTAAAACAACAATTAAACGAGAAAGGTTTTAACCTAAATCTTATCGGCCGTGCTCGTAAAATGAAA
ATCGTTTTAGACCAAGAATTTGTCATTGAAAAACTAAAAGTAAATGATGACATCCTGACTTATAAACAAGTAGAAAATAG
TTTTACCCAACCAAACGGTGTTGTAGCTCAAAAAATGTTGGAATGGGCCGTTGATTGTACGCAAGACAGCCAAGGCGACT
TATTGGAACTTTATTGTGGTAATGGTAATTTTTCATTAGCATTAGCAAAAAACTTTGATCGCGTATTAGCAACCGAACTT
GCAAAGCCATCCGTAGAATCTGCTCAATATAATATTGCAGCCAATAATATAGACAATGTACAAATTGTTCGTATGTCGGC
AGAAGACTTTACAGATGCAATGGAAGGAAAGCGTGAATTCCGACGTTTGAAAGATCAAAATGTAGATTTAAAAAGCTATA
ACTGCAATACCATCTTTGTCGATCCACCACGTTCTGGCATGGACGAAGGCACTTGTAAGATGGTACAAGGCTATGAGCGT
ATTATGTACATTTCTTGTAATCCTGACACTCTAAAAGAGAACCTTGCTATTTTAAGTAAAACACACAACATTACTCGTTT
TGCTTTATTTGATCAGTTCCCATATACCCACCATATGGAAGCCGGCATATTCTTAGAGCGCAAATAA

Upstream 100 bases:

>100_bases
TCGATCGAATTACTTTCGGCCAATCACACACAAATGCTGGACAACAGATCTCTATCGAATAAAATCGGCGCTCTTATTTT
GAGCAGCGTGAGGCAAGACA

Downstream 100 bases:

>100_bases
ACACTCGATTTAATCAATTAAAAAAGGCTCATCACATTATGAGCCTTTTTTTTATTTTCGTTAAATTAAGATTTACAAAT
CTGTTGGTGCCTCTTTTTTC

Product: tRNA (uracil-5-)-methyltransferase

Products: NA

Alternate protein names: tRNA(M-5-U54)-methyltransferase; RUMT

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTQPVMNPENYQVQLDEKTEALSAMFSEFDVPELEVFSSPAENYRMRAEFRIWHEGDDMYYVMFDQKTKEKYRVDYFLPA
TRLINDLMPLLAEAIKGSKTLRYKMFQVDFLSTLSGEILVSMLYHRQLDDAWKEEAAVLKQQLNEKGFNLNLIGRARKMK
IVLDQEFVIEKLKVNDDILTYKQVENSFTQPNGVVAQKMLEWAVDCTQDSQGDLLELYCGNGNFSLALAKNFDRVLATEL
AKPSVESAQYNIAANNIDNVQIVRMSAEDFTDAMEGKREFRRLKDQNVDLKSYNCNTIFVDPPRSGMDEGTCKMVQGYER
IMYISCNPDTLKENLAILSKTHNITRFALFDQFPYTHHMEAGIFLERK

Sequences:

>Translated_368_residues
MTQPVMNPENYQVQLDEKTEALSAMFSEFDVPELEVFSSPAENYRMRAEFRIWHEGDDMYYVMFDQKTKEKYRVDYFLPA
TRLINDLMPLLAEAIKGSKTLRYKMFQVDFLSTLSGEILVSMLYHRQLDDAWKEEAAVLKQQLNEKGFNLNLIGRARKMK
IVLDQEFVIEKLKVNDDILTYKQVENSFTQPNGVVAQKMLEWAVDCTQDSQGDLLELYCGNGNFSLALAKNFDRVLATEL
AKPSVESAQYNIAANNIDNVQIVRMSAEDFTDAMEGKREFRRLKDQNVDLKSYNCNTIFVDPPRSGMDEGTCKMVQGYER
IMYISCNPDTLKENLAILSKTHNITRFALFDQFPYTHHMEAGIFLERK
>Mature_367_residues
TQPVMNPENYQVQLDEKTEALSAMFSEFDVPELEVFSSPAENYRMRAEFRIWHEGDDMYYVMFDQKTKEKYRVDYFLPAT
RLINDLMPLLAEAIKGSKTLRYKMFQVDFLSTLSGEILVSMLYHRQLDDAWKEEAAVLKQQLNEKGFNLNLIGRARKMKI
VLDQEFVIEKLKVNDDILTYKQVENSFTQPNGVVAQKMLEWAVDCTQDSQGDLLELYCGNGNFSLALAKNFDRVLATELA
KPSVESAQYNIAANNIDNVQIVRMSAEDFTDAMEGKREFRRLKDQNVDLKSYNCNTIFVDPPRSGMDEGTCKMVQGYERI
MYISCNPDTLKENLAILSKTHNITRFALFDQFPYTHHMEAGIFLERK

Specific function: Catalyzes the formation of 5-methyl-uridine at position 54 (M-5-U54) in all tRNAs

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RNA M5U methyltransferase family. TrmA subfamily

Homologues:

Organism=Homo sapiens, GI34222389, Length=220, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-14,
Organism=Homo sapiens, GI51173878, Length=220, Percent_Identity=27.2727272727273, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1790403, Length=368, Percent_Identity=59.7826086956522, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI1789148, Length=188, Percent_Identity=32.9787234042553, Blast_Score=86, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24666579, Length=195, Percent_Identity=28.2051282051282, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): TRMA_ALISL (B6ENT4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264217.1
- ProteinModelPortal:   B6ENT4
- GeneID:   6988198
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2887
- HOGENOM:   HBG608402
- OMA:   NEVRPFR
- ProtClustDB:   PRK05031
- HAMAP:   MF_01011
- InterPro:   IPR011869
- InterPro:   IPR010280
- TIGRFAMs:   TIGR02143

Pfam domain/function: PF05958 tRNA_U5-meth_tr

EC number: =2.1.1.35

Molecular weight: Translated: 42645; Mature: 42514

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 326-326 BINDING 190-190 BINDING 218-218 BINDING 223-223 BINDING 239-239 BINDING 301-301

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQPVMNPENYQVQLDEKTEALSAMFSEFDVPELEVFSSPAENYRMRAEFRIWHEGDDMY
CCCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHCCCCHHCEEEEEEEEEECCCCEE
YVMFDQKTKEKYRVDYFLPATRLINDLMPLLAEAIKGSKTLRYKMFQVDFLSTLSGEILV
EEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHH
SMLYHRQLDDAWKEEAAVLKQQLNEKGFNLNLIGRARKMKIVLDQEFVIEKLKVNDDILT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHHHCCCCCHHH
YKQVENSFTQPNGVVAQKMLEWAVDCTQDSQGDLLELYCGNGNFSLALAKNFDRVLATEL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVESAQYNIAANNIDNVQIVRMSAEDFTDAMEGKREFRRLKDQNVDLKSYNCNTIFV
CCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEE
DPPRSGMDEGTCKMVQGYERIMYISCNPDTLKENLAILSKTHNITRFALFDQFPYTHHME
CCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECC
AGIFLERK
CCEEEECC
>Mature Secondary Structure 
TQPVMNPENYQVQLDEKTEALSAMFSEFDVPELEVFSSPAENYRMRAEFRIWHEGDDMY
CCCCCCCCCCEEEECCHHHHHHHHHHHCCCCCHHHHCCCCHHCEEEEEEEEEECCCCEE
YVMFDQKTKEKYRVDYFLPATRLINDLMPLLAEAIKGSKTLRYKMFQVDFLSTLSGEILV
EEEEECCCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCEEEEEEEEEHHHHHHHHHHHH
SMLYHRQLDDAWKEEAAVLKQQLNEKGFNLNLIGRARKMKIVLDQEFVIEKLKVNDDILT
HHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCEEEEEEECHHHHHHHHCCCCCHHH
YKQVENSFTQPNGVVAQKMLEWAVDCTQDSQGDLLELYCGNGNFSLALAKNFDRVLATEL
HHHHHHCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCEEEEEECCHHHHHHHHH
AKPSVESAQYNIAANNIDNVQIVRMSAEDFTDAMEGKREFRRLKDQNVDLKSYNCNTIFV
CCCCCCCCEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEE
DPPRSGMDEGTCKMVQGYERIMYISCNPDTLKENLAILSKTHNITRFALFDQFPYTHHME
CCCCCCCCCCHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEECC
AGIFLERK
CCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA