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The map label for this gene is amiB [H]

Identifier: 209696186

GI number: 209696186

Start: 3010386

End: 3012125

Strand: Reverse

Name: amiB [H]

Synonym: VSAL_I2780

Alternate gene names: 209696186

Gene position: 3012125-3010386 (Counterclockwise)

Preceding gene: 209696187

Following gene: 209696185

Centisome position: 90.59

GC content: 38.51

Gene sequence:

>1740_bases
GTGTTAATCGGTAAATATCTTCGTACAATTGGGTGTGCATTACTTGTATTTATATCTACGAGTGTTTTTTTTGTTTCTGC
AAATACATTAGAAGGCGTTCGTGTTTGGCCTTCGCCAGATGAAACTCGTATCGTTATTGATCTGAATTCAGAAGCGAACT
ATAGCTATTTTACATTGAGTAGCCCTTCTCGTTTAGTTATCGATCTTAAAAATACCGACTTGAAAGCAAAATTACCGCTA
ACGGTTAGTGATAGCAATGTTTTAAGTAAAATCCGTAAGAGCTCACCTCCTTCAAAGGGGACGACTCGTTTGGTTTTTGA
ACTTAAGCATTCTGTCGAAGCTAAGTTATTTAAATTAGCCCCTACGCCTGGTGGTCAATATGGTCATCGTTTGGTTATGG
ACCTACCTCATAACGCAAACAATAAAGCGGAAGTAAATAAACCTATTCCCAATAAGCCATCAACCCCAATTATCAATTCA
AATATGAATCATATAATAGGGAATGGAGAAATTGTTGTCGCGATTGATGCGGGACATGGTGGTGAAGATCCTGGCTCTAT
TGGGCCAACTCGAAAATATGAAAAGCACGCTACTCTCGCGATTTCTAAGAAAGTGGTTGCACGAATTAATGCTCAGCCTG
GAATGAGAGCGGTATTAACTCGAAGTGGTGATTATTTCGTTAACTTAAATAAACGAACGGAAATTGCTCGAAAAAATAAA
TCGCACTTACTTGTTTCTATTCATGCGGACAGCTTTCGCTCACCAAAGCCTCGCGGGGGTTCTGTGTTTGTGCTCAATAC
ACGTCGAGCGAATACAGAAATTGGTCGTTGGGTTGAAAAGCATGAGAAACAATCTGAATTGCTTGGTGGTGCAGGTGATG
TATTAGCGAAAAACAGTAATGATAGAAACGTGAGTCAAACATTATTGGATTTGCAATTCAGCCATTCTCAGAAGGAAGGG
TATAAGTTAGCCGTTGATGTTCTAGGAGAAATGGGCAAAGTCATTAAGCTTCATAAAAAAGCACCTGTTAATGCGAGTTT
GGCTGTTCTTAAATCTCCGGATATTCCTTCAATCTTGATTGAAACTGGATTTATTTCAAATCCAACAGAAGAGAAACAAC
TATTTCAACGAGGTCATCAAGACAAAATAGCGAGAGCTATTTCAAATGCAATCATTCGTTACCTTAATGAAAATCCACCT
CAAGGAAGTCTTTTAGCTGCACAAGAGCAACAGAGTGTACATAAAGTACGTTCGGGTGAGTCGTTATCATTGATTGCTAA
AAAGTACGGTACGACAACGGCAAAATTAAAGAGTGTAAATAAACTTAAAAGTACGAGTTTATCGGTTGGTCAACTGCTAA
CTATCCCAAGTAACAAACCGGTTTATGTTGCGCCAAAAACAAATACATCAAACGTCAGTACATCAACAACGCAATCTCGT
AAAATCATTACTCATAAAGTTCGCTCTGGTGAGTACTTAGGCAAGATTGCCGTAAAATATAAGGTATCGACGAGCAGCAT
TCGCTCATTAAATCGATTAAAGTCAGATACTTTATTTGTTGGGCAAAAATTGAAAGTGAGCATTGTTGCTCCTATTCAAA
AGCATACGGTGAAACGTGGTGAATTTTTAGGTAAAATTGCATCTAAATATGGTGTTACTGTATCGAGTATACGAACAGCC
AATAAATTACGTTCAGATGAGCTAGCTGTCGGACAGGTGCTTATTATCCCAACCAGTTAA

Upstream 100 bases:

>100_bases
CTGATATCGATATTGAGCTTCGCTATGATGGTGAGGCTCGTCATGTCGTGATCACGGGTAATAATGAATATGGCTGCAGT
TTACTTCAACGGTTGGATTT

Downstream 100 bases:

>100_bases
TCAAGGATATGGTTATGCCAATTCAAATCTTACCCGCTCGACTGGCCAACCAAATTGCGGCGGGTGAGGTTGTGGAACGC
CCCGCTTCGGTTGTCAAAGA

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 579; Mature: 579

Protein sequence:

>579_residues
MLIGKYLRTIGCALLVFISTSVFFVSANTLEGVRVWPSPDETRIVIDLNSEANYSYFTLSSPSRLVIDLKNTDLKAKLPL
TVSDSNVLSKIRKSSPPSKGTTRLVFELKHSVEAKLFKLAPTPGGQYGHRLVMDLPHNANNKAEVNKPIPNKPSTPIINS
NMNHIIGNGEIVVAIDAGHGGEDPGSIGPTRKYEKHATLAISKKVVARINAQPGMRAVLTRSGDYFVNLNKRTEIARKNK
SHLLVSIHADSFRSPKPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRNVSQTLLDLQFSHSQKEG
YKLAVDVLGEMGKVIKLHKKAPVNASLAVLKSPDIPSILIETGFISNPTEEKQLFQRGHQDKIARAISNAIIRYLNENPP
QGSLLAAQEQQSVHKVRSGESLSLIAKKYGTTTAKLKSVNKLKSTSLSVGQLLTIPSNKPVYVAPKTNTSNVSTSTTQSR
KIITHKVRSGEYLGKIAVKYKVSTSSIRSLNRLKSDTLFVGQKLKVSIVAPIQKHTVKRGEFLGKIASKYGVTVSSIRTA
NKLRSDELAVGQVLIIPTS

Sequences:

>Translated_579_residues
MLIGKYLRTIGCALLVFISTSVFFVSANTLEGVRVWPSPDETRIVIDLNSEANYSYFTLSSPSRLVIDLKNTDLKAKLPL
TVSDSNVLSKIRKSSPPSKGTTRLVFELKHSVEAKLFKLAPTPGGQYGHRLVMDLPHNANNKAEVNKPIPNKPSTPIINS
NMNHIIGNGEIVVAIDAGHGGEDPGSIGPTRKYEKHATLAISKKVVARINAQPGMRAVLTRSGDYFVNLNKRTEIARKNK
SHLLVSIHADSFRSPKPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRNVSQTLLDLQFSHSQKEG
YKLAVDVLGEMGKVIKLHKKAPVNASLAVLKSPDIPSILIETGFISNPTEEKQLFQRGHQDKIARAISNAIIRYLNENPP
QGSLLAAQEQQSVHKVRSGESLSLIAKKYGTTTAKLKSVNKLKSTSLSVGQLLTIPSNKPVYVAPKTNTSNVSTSTTQSR
KIITHKVRSGEYLGKIAVKYKVSTSSIRSLNRLKSDTLFVGQKLKVSIVAPIQKHTVKRGEFLGKIASKYGVTVSSIRTA
NKLRSDELAVGQVLIIPTS
>Mature_579_residues
MLIGKYLRTIGCALLVFISTSVFFVSANTLEGVRVWPSPDETRIVIDLNSEANYSYFTLSSPSRLVIDLKNTDLKAKLPL
TVSDSNVLSKIRKSSPPSKGTTRLVFELKHSVEAKLFKLAPTPGGQYGHRLVMDLPHNANNKAEVNKPIPNKPSTPIINS
NMNHIIGNGEIVVAIDAGHGGEDPGSIGPTRKYEKHATLAISKKVVARINAQPGMRAVLTRSGDYFVNLNKRTEIARKNK
SHLLVSIHADSFRSPKPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSNDRNVSQTLLDLQFSHSQKEG
YKLAVDVLGEMGKVIKLHKKAPVNASLAVLKSPDIPSILIETGFISNPTEEKQLFQRGHQDKIARAISNAIIRYLNENPP
QGSLLAAQEQQSVHKVRSGESLSLIAKKYGTTTAKLKSVNKLKSTSLSVGQLLTIPSNKPVYVAPKTNTSNVSTSTTQSR
KIITHKVRSGEYLGKIAVKYKVSTSSIRSLNRLKSDTLFVGQKLKVSIVAPIQKHTVKRGEFLGKIASKYGVTVSSIRTA
NKLRSDELAVGQVLIIPTS

Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]

COG id: COG0860

COG function: function code M; N-acetylmuramoyl-L-alanine amidase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]

Homologues:

Organism=Escherichia coli, GI1790611, Length=434, Percent_Identity=37.0967741935484, Blast_Score=270, Evalue=1e-73,
Organism=Escherichia coli, GI87082163, Length=416, Percent_Identity=34.6153846153846, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1788776, Length=230, Percent_Identity=40, Blast_Score=159, Evalue=7e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002508 [H]

Pfam domain/function: PF01520 Amidase_3 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 63212; Mature: 63212

Theoretical pI: Translated: 10.83; Mature: 10.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIGKYLRTIGCALLVFISTSVFFVSANTLEGVRVWPSPDETRIVIDLNSEANYSYFTLS
CCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEECCCCCCCEEEEEECCCCCEEEEEEC
SPSRLVIDLKNTDLKAKLPLTVSDSNVLSKIRKSSPPSKGTTRLVFELKHSVEAKLFKLA
CCCEEEEEEECCCEEEEEEEEECCHHHHHHHHHCCCCCCCCEEEEEEEHHCCCEEEEEEC
PTPGGQYGHRLVMDLPHNANNKAEVNKPIPNKPSTPIINSNMNHIIGNGEIVVAIDAGHG
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCEEEEEEECCCC
GEDPGSIGPTRKYEKHATLAISKKVVARINAQPGMRAVLTRSGDYFVNLNKRTEIARKNK
CCCCCCCCCCCCHHCHHHHEEHHHHHHEECCCCCCEEEEECCCCEEEEECCHHHHHHCCC
SHLLVSIHADSFRSPKPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSN
CEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCC
DRNVSQTLLDLQFSHSQKEGYKLAVDVLGEMGKVIKLHKKAPVNASLAVLKSPDIPSILI
CCCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCHHEEEECCCCCCCEEEEEECCCCCEEEE
ETGFISNPTEEKQLFQRGHQDKIARAISNAIIRYLNENPPQGSLLAAQEQQSVHKVRSGE
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCC
SLSLIAKKYGTTTAKLKSVNKLKSTSLSVGQLLTIPSNKPVYVAPKTNTSNVSTSTTQSR
CCEEHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCC
KIITHKVRSGEYLGKIAVKYKVSTSSIRSLNRLKSDTLFVGQKLKVSIVAPIQKHTVKRG
HHEEEEECCCCEEEEEEEEEEECHHHHHHHHHHCCCEEEECCEEEEEEECCHHHHHHHHH
EFLGKIASKYGVTVSSIRTANKLRSDELAVGQVLIIPTS
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEECEEEEEECC
>Mature Secondary Structure
MLIGKYLRTIGCALLVFISTSVFFVSANTLEGVRVWPSPDETRIVIDLNSEANYSYFTLS
CCHHHHHHHHHHHHHHHHHCEEEEEEECCCCCEEECCCCCCCEEEEEECCCCCEEEEEEC
SPSRLVIDLKNTDLKAKLPLTVSDSNVLSKIRKSSPPSKGTTRLVFELKHSVEAKLFKLA
CCCEEEEEEECCCEEEEEEEEECCHHHHHHHHHCCCCCCCCEEEEEEEHHCCCEEEEEEC
PTPGGQYGHRLVMDLPHNANNKAEVNKPIPNKPSTPIINSNMNHIIGNGEIVVAIDAGHG
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCEECCCCCEEECCCEEEEEEECCCC
GEDPGSIGPTRKYEKHATLAISKKVVARINAQPGMRAVLTRSGDYFVNLNKRTEIARKNK
CCCCCCCCCCCCHHCHHHHEEHHHHHHEECCCCCCEEEEECCCCEEEEECCHHHHHHCCC
SHLLVSIHADSFRSPKPRGGSVFVLNTRRANTEIGRWVEKHEKQSELLGGAGDVLAKNSN
CEEEEEEECCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCEEECCCC
DRNVSQTLLDLQFSHSQKEGYKLAVDVLGEMGKVIKLHKKAPVNASLAVLKSPDIPSILI
CCCHHHHHHHHHCCCCCCCCCEEEHHHHHHCCHHEEEECCCCCCCEEEEEECCCCCEEEE
ETGFISNPTEEKQLFQRGHQDKIARAISNAIIRYLNENPPQGSLLAAQEQQSVHKVRSGE
ECCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHHHHCCC
SLSLIAKKYGTTTAKLKSVNKLKSTSLSVGQLLTIPSNKPVYVAPKTNTSNVSTSTTQSR
CCEEHHHHHCCCHHHHHHHHHHHHCCCCCCEEEECCCCCCEEEECCCCCCCCCCCCCCCC
KIITHKVRSGEYLGKIAVKYKVSTSSIRSLNRLKSDTLFVGQKLKVSIVAPIQKHTVKRG
HHEEEEECCCCEEEEEEEEEEECHHHHHHHHHHCCCEEEECCEEEEEEECCHHHHHHHHH
EFLGKIASKYGVTVSSIRTANKLRSDELAVGQVLIIPTS
HHHHHHHHHHCCCHHHHHHHHHHCCCCEEECEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11677609; 2676972 [H]