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The map label for this gene is miaA

Identifier: 209696184

GI number: 209696184

Start: 3007479

End: 3008420

Strand: Reverse

Name: miaA

Synonym: VSAL_I2778

Alternate gene names: 209696184

Gene position: 3008420-3007479 (Counterclockwise)

Preceding gene: 209696185

Following gene: 209696183

Centisome position: 90.47

GC content: 41.61

Gene sequence:

>942_bases
ATGAATAAAGCATTACCTCAAGCCATCTTTTTGATGGGACCAACTGCATCAGGGAAAACGGACTTAGCAATTCAATTGCG
AAAACGTTTTCCTGTGGAGTTGATAAGCGTAGATTCAGCCCTTATCTATAAAGGTATGGATATTGGTACGGCGAAACCAA
ATGAAACGGAACTTTTACAGGCTCCTCATCGTCTTATTGATATTCTCGATCCGGCAGAGAGCTATTCAGTTGCTGAATTT
CGTCGTGATGCCTTAAAAGAGATGGAAGATATTGTTGCTCAAGGAAAGATACCTTTATTAGTAGGCGGTACAATGTTGTA
CTACAAAGCACTGCTAGAAGGTCTTTCACCATTACCAGCAGCGGATGCTGACATTAGAGCTCAAATTGAGCAAGAAGCGG
AAACATTGGGGTGGGAGGCGATGCATGATCAGTTAAAAGAGATTGATCCTGTATCCGCTGAGAGAATTCATCCTAATGAT
CCACAACGACTTTCCCGAGCGTTGGAAGTGTTTCGAATTTCGGGTAAAACATTAACAGAATTAACCCAAGTTAAAGGTGA
TGCTTTGCCTTATCAAGTGCATCAATTTGCGATTGCACCTAAAGAAAGAGCAGAAATTCACCGTCGTATTGAACTGCGTT
TTGATAATATGATGAAAGGCGGTTTTGAAGAAGAAGTTCGTTCTCTTTATGAACGAGATGATCTTCATGCCGATTTGCCA
TCAATTCGTTGCGTAGGTTATCGACAAATGTGGGAGTATTTTGATGGGGAAGGCACATTAGATGACGCGATTTTCCGCGG
TATTTGTGCGACTCGTCAATTGGCCAAGAGACAAATCACCTGGCTACGTAGCTGGAAAGACTTAACTTGGCTGGATAGTG
ACAATATTGATGGTGCCCTACAAACGATTTCTGATCGTTTAGATAAGAAACAAGCACAATAA

Upstream 100 bases:

>100_bases
GTGATGGAATTAGAGCAGTTGTGGGGTAATGAATTATCTCAATTTAATAATAAACTGCTAAAAAACATAGATATTACTCA
GGCGATACAAGCATTTAGTC

Downstream 100 bases:

>100_bases
TGTATCAATGTTGTTTAACTTCAGAGCGTTTAGTCATAAGCTAAAGACTAATAGCTAAATAAAATTACAATTACAAACCA
AAAAGGAAAAAAAAATGGCT

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MNKALPQAIFLMGPTASGKTDLAIQLRKRFPVELISVDSALIYKGMDIGTAKPNETELLQAPHRLIDILDPAESYSVAEF
RRDALKEMEDIVAQGKIPLLVGGTMLYYKALLEGLSPLPAADADIRAQIEQEAETLGWEAMHDQLKEIDPVSAERIHPND
PQRLSRALEVFRISGKTLTELTQVKGDALPYQVHQFAIAPKERAEIHRRIELRFDNMMKGGFEEEVRSLYERDDLHADLP
SIRCVGYRQMWEYFDGEGTLDDAIFRGICATRQLAKRQITWLRSWKDLTWLDSDNIDGALQTISDRLDKKQAQ

Sequences:

>Translated_313_residues
MNKALPQAIFLMGPTASGKTDLAIQLRKRFPVELISVDSALIYKGMDIGTAKPNETELLQAPHRLIDILDPAESYSVAEF
RRDALKEMEDIVAQGKIPLLVGGTMLYYKALLEGLSPLPAADADIRAQIEQEAETLGWEAMHDQLKEIDPVSAERIHPND
PQRLSRALEVFRISGKTLTELTQVKGDALPYQVHQFAIAPKERAEIHRRIELRFDNMMKGGFEEEVRSLYERDDLHADLP
SIRCVGYRQMWEYFDGEGTLDDAIFRGICATRQLAKRQITWLRSWKDLTWLDSDNIDGALQTISDRLDKKQAQ
>Mature_313_residues
MNKALPQAIFLMGPTASGKTDLAIQLRKRFPVELISVDSALIYKGMDIGTAKPNETELLQAPHRLIDILDPAESYSVAEF
RRDALKEMEDIVAQGKIPLLVGGTMLYYKALLEGLSPLPAADADIRAQIEQEAETLGWEAMHDQLKEIDPVSAERIHPND
PQRLSRALEVFRISGKTLTELTQVKGDALPYQVHQFAIAPKERAEIHRRIELRFDNMMKGGFEEEVRSLYERDDLHADLP
SIRCVGYRQMWEYFDGEGTLDDAIFRGICATRQLAKRQITWLRSWKDLTWLDSDNIDGALQTISDRLDKKQAQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A)

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family

Homologues:

Organism=Homo sapiens, GI31581534, Length=319, Percent_Identity=28.5266457680251, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI1790613, Length=296, Percent_Identity=72.972972972973, Blast_Score=461, Evalue=1e-131,
Organism=Caenorhabditis elegans, GI25144712, Length=297, Percent_Identity=30.3030303030303, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6324848, Length=289, Percent_Identity=28.3737024221453, Blast_Score=126, Evalue=4e-30,
Organism=Drosophila melanogaster, GI24649830, Length=270, Percent_Identity=30.7407407407407, Blast_Score=124, Evalue=8e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAA_ALISL (B6EMQ8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264114.1
- ProteinModelPortal:   B6EMQ8
- GeneID:   6988657
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2778
- HOGENOM:   HBG559459
- OMA:   VNADSMQ
- ProtClustDB:   PRK00091
- HAMAP:   MF_00185
- InterPro:   IPR002627
- InterPro:   IPR018022
- PANTHER:   PTHR11088
- TIGRFAMs:   TIGR00174

Pfam domain/function: PF01715 IPPT

EC number: =2.5.1.75

Molecular weight: Translated: 35547; Mature: 35547

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKALPQAIFLMGPTASGKTDLAIQLRKRFPVELISVDSALIYKGMDIGTAKPNETELLQ
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDILDPAESYSVAEFRRDALKEMEDIVAQGKIPLLVGGTMLYYKALLEGLSPLPA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADADIRAQIEQEAETLGWEAMHDQLKEIDPVSAERIHPNDPQRLSRALEVFRISGKTLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQVKGDALPYQVHQFAIAPKERAEIHRRIELRFDNMMKGGFEEEVRSLYERDDLHADLP
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
SIRCVGYRQMWEYFDGEGTLDDAIFRGICATRQLAKRQITWLRSWKDLTWLDSDNIDGAL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHH
QTISDRLDKKQAQ
HHHHHHHHHHCCC
>Mature Secondary Structure
MNKALPQAIFLMGPTASGKTDLAIQLRKRFPVELISVDSALIYKGMDIGTAKPNETELLQ
CCCCCCCEEEEECCCCCCCCHHHHHHHHHCCEEEEECCHHHHHCCCCCCCCCCCHHHHHH
APHRLIDILDPAESYSVAEFRRDALKEMEDIVAQGKIPLLVGGTMLYYKALLEGLSPLPA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHCCCCCCC
ADADIRAQIEQEAETLGWEAMHDQLKEIDPVSAERIHPNDPQRLSRALEVFRISGKTLTE
CCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
LTQVKGDALPYQVHQFAIAPKERAEIHRRIELRFDNMMKGGFEEEVRSLYERDDLHADLP
HHHHCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCC
SIRCVGYRQMWEYFDGEGTLDDAIFRGICATRQLAKRQITWLRSWKDLTWLDSDNIDGAL
CHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCHHH
QTISDRLDKKQAQ
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA