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The map label for this gene is hslU

Identifier: 209696145

GI number: 209696145

Start: 2964469

End: 2965803

Strand: Reverse

Name: hslU

Synonym: VSAL_I2739

Alternate gene names: 209696145

Gene position: 2965803-2964469 (Counterclockwise)

Preceding gene: 209696146

Following gene: 209696143

Centisome position: 89.19

GC content: 42.25

Gene sequence:

>1335_bases
ATGTCTGAAATGACTCCTCGTGAAATTGTACACGAATTAGATAGCCATATTATTGGTCAAGATAAAGCAAAACGATCAGT
CGCCATTGCATTACGTAACCGCTGGCGTCGAATGCAACTTCAACCAGAATTACGCGCAGAAGTAACGCCTAAAAACATTT
TAATGATCGGACCTACAGGTGTCGGTAAAACAGAGATTGCTCGTCGTTTAGCAAAACTAGCCAATGCACCGTTCATTAAA
GTAGAAGCGACTAAATTCACAGAAGTCGGTTATGTAGGCAAAGAAGTCGACAGCATCATTCGTGATTTAACCGATGTCGC
GATCAAAATGACACATCAACAGGCGGTAGAAAAAGTAAAATTCCGCGCTGAAGAGCAAGCTGAAGATCGTATCCTTGATA
TTTTATTACCACCCGCTCGCGATGCATGGGGTAAGAATGAAGAAGGTGATAATGACTCTGGCACTCGTCAATCTTTCCGT
AAGAAATTGCGTGAAGGCAAATTAGACGATAAAGAGATTGATGTTGATGTTGCTGCACCTCAAGTGGGTGTCGAAATCAT
GGCTCCTCCAGGCATGGAAGAAATGACGAACCAACTTCAGGGCATGTTTCAGAACCTATCTGGTGGCGAAACAACGAAAA
AGCGCAAGATGAAAATCAAAGATGCGCTTAAAGCCCTTGCTGAAGAAGAAGGCGCTAAATTAGTCAACCCTGAAGAGCTA
AAAGAACAAGCCATATTTAACGTAGAAAATCACGGCATCGTCTTTATTGATGAGATCGATAAAATCTGCAAAGGATCAAA
TTCTCAATCTGGTGATGTATCTCGCGAAGGCGTTCAACGTGACCTTCTTCCATTAGTGGAAGGCAGCACAGTAAGCACGA
AACACGGCATGGTTAGAACTGATCACATGCTATTCATCACATCAGGTGCATTCCAAATGGCGAAACCATCCGATTTGATC
CCTGAATTACAAGGTCGCTTACCGATCCGTGTTGAGCTAGAAGCGCTAACGGCAAATGACTTTAAGCGTATTTTAACCGA
GCCAAATGCCTCACTAACTGAGCAATACATCGCTCTATTAGCGACTGAAAATGTCAATATCGAATTTACTGAAGATGGTA
TTAGTCGTATTGCAGAGTCTGCATTTAGAGTTAATGAAACGACAGAGAACATCGGTGCTCGTCGTTTGCACACCGTAATG
GAACGTCTAATGGAAGAGATTTCTTATGATGCTTCAGAGAAAGGCGGTGACAGCTTAACGGTAGATGACGCTTATGTAAC
CTCTCGTTTAGGTGAATTAATTGAAAATGAAGACTTAAGTCGCTTCATTTTGTAA

Upstream 100 bases:

>100_bases
TGCAGTGAAAGCACTGACTATCGCTGGTGATACTGATGTATACACCAATCATAACCATACCGTTGAAGTTCTTGATACCA
ACAAATAGTAAGGAATACCC

Downstream 100 bases:

>100_bases
TCATTCCCCCTTTTAAAAAAGGCCCTGTTCAACACTGAACGGGGCCTTTTTACATTAATTTTTCAAATGTAATTACGCAA
TGATTTCCCAGCTGTGAGTC

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MSEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLQPELRAEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVDSIIRDLTDVAIKMTHQQAVEKVKFRAEEQAEDRILDILLPPARDAWGKNEEGDNDSGTRQSFR
KKLREGKLDDKEIDVDVAAPQVGVEIMAPPGMEEMTNQLQGMFQNLSGGETTKKRKMKIKDALKALAEEEGAKLVNPEEL
KEQAIFNVENHGIVFIDEIDKICKGSNSQSGDVSREGVQRDLLPLVEGSTVSTKHGMVRTDHMLFITSGAFQMAKPSDLI
PELQGRLPIRVELEALTANDFKRILTEPNASLTEQYIALLATENVNIEFTEDGISRIAESAFRVNETTENIGARRLHTVM
ERLMEEISYDASEKGGDSLTVDDAYVTSRLGELIENEDLSRFIL

Sequences:

>Translated_444_residues
MSEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLQPELRAEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIK
VEATKFTEVGYVGKEVDSIIRDLTDVAIKMTHQQAVEKVKFRAEEQAEDRILDILLPPARDAWGKNEEGDNDSGTRQSFR
KKLREGKLDDKEIDVDVAAPQVGVEIMAPPGMEEMTNQLQGMFQNLSGGETTKKRKMKIKDALKALAEEEGAKLVNPEEL
KEQAIFNVENHGIVFIDEIDKICKGSNSQSGDVSREGVQRDLLPLVEGSTVSTKHGMVRTDHMLFITSGAFQMAKPSDLI
PELQGRLPIRVELEALTANDFKRILTEPNASLTEQYIALLATENVNIEFTEDGISRIAESAFRVNETTENIGARRLHTVM
ERLMEEISYDASEKGGDSLTVDDAYVTSRLGELIENEDLSRFIL
>Mature_443_residues
SEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLQPELRAEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKV
EATKFTEVGYVGKEVDSIIRDLTDVAIKMTHQQAVEKVKFRAEEQAEDRILDILLPPARDAWGKNEEGDNDSGTRQSFRK
KLREGKLDDKEIDVDVAAPQVGVEIMAPPGMEEMTNQLQGMFQNLSGGETTKKRKMKIKDALKALAEEEGAKLVNPEELK
EQAIFNVENHGIVFIDEIDKICKGSNSQSGDVSREGVQRDLLPLVEGSTVSTKHGMVRTDHMLFITSGAFQMAKPSDLIP
ELQGRLPIRVELEALTANDFKRILTEPNASLTEQYIALLATENVNIEFTEDGISRIAESAFRVNETTENIGARRLHTVME
RLMEEISYDASEKGGDSLTVDDAYVTSRLGELIENEDLSRFIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Escherichia coli, GI1790366, Length=444, Percent_Identity=77.2522522522522, Blast_Score=700, Evalue=0.0,
Organism=Escherichia coli, GI1786642, Length=100, Percent_Identity=45, Blast_Score=92, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6319704, Length=490, Percent_Identity=25.1020408163265, Blast_Score=100, Evalue=4e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_ALISL (B6EML9)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002264075.1
- GeneID:   6987903
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2739
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 49650; Mature: 49519

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: BINDING 18-18 BINDING 257-257 BINDING 322-322 BINDING 394-394

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLQPELRAEVTPKNILMIGPTG
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDVAIKMTHQQAVEKVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEEQAEDRILDILLPPARDAWGKNEEGDNDSGTRQSFRKKLREGKLDDKEIDVDVAAP
HHHHHHHHHHEEHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECC
QVGVEIMAPPGMEEMTNQLQGMFQNLSGGETTKKRKMKIKDALKALAEEEGAKLVNPEEL
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KEQAIFNVENHGIVFIDEIDKICKGSNSQSGDVSREGVQRDLLPLVEGSTVSTKHGMVRT
HHHHHEEECCCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
DHMLFITSGAFQMAKPSDLIPELQGRLPIRVELEALTANDFKRILTEPNASLTEQYIALL
CCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHH
ATENVNIEFTEDGISRIAESAFRVNETTENIGARRLHTVMERLMEEISYDASEKGGDSLT
EECCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VDDAYVTSRLGELIENEDLSRFIL
ECHHHHHHHHHHHHCCCCHHHHCC
>Mature Secondary Structure 
SEMTPREIVHELDSHIIGQDKAKRSVAIALRNRWRRMQLQPELRAEVTPKNILMIGPTG
CCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCEEEECCCC
VGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRDLTDVAIKMTHQQAVEKVK
CCHHHHHHHHHHHHCCCEEEEECCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FRAEEQAEDRILDILLPPARDAWGKNEEGDNDSGTRQSFRKKLREGKLDDKEIDVDVAAP
HHHHHHHHHHEEHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEEEEEECC
QVGVEIMAPPGMEEMTNQLQGMFQNLSGGETTKKRKMKIKDALKALAEEEGAKLVNPEEL
CCCEEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
KEQAIFNVENHGIVFIDEIDKICKGSNSQSGDVSREGVQRDLLPLVEGSTVSTKHGMVRT
HHHHHEEECCCCEEEEHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEE
DHMLFITSGAFQMAKPSDLIPELQGRLPIRVELEALTANDFKRILTEPNASLTEQYIALL
CCEEEEECCCCCCCCCHHHHHHHHCCCCEEEEEEECCHHHHHHHHCCCCHHHHHHHHHHH
ATENVNIEFTEDGISRIAESAFRVNETTENIGARRLHTVMERLMEEISYDASEKGGDSLT
EECCCEEEECHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEE
VDDAYVTSRLGELIENEDLSRFIL
ECHHHHHHHHHHHHCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA