Click here to switch to the map view.

The map label for this gene is gph [C]

Identifier: 209696133

GI number: 209696133

Start: 2953235

End: 2953918

Strand: Direct

Name: gph [C]

Synonym: VSAL_I2727

Alternate gene names: 209696133

Gene position: 2953235-2953918 (Clockwise)

Preceding gene: 209696132

Following gene: 209696134

Centisome position: 88.81

GC content: 41.52

Gene sequence:

>684_bases
ATGTTTGAAAACATCAAACTGATCGCGTTTGATTTGGATGGAACACTGCTAGATAGTGTTCCAGACTTAGCTCGTGCGGT
TGATCTTGCTATGCAAGACCTAGATTACCCTGGCGTTACATTAGAGCAAGCTTCTCATTGGATAGGCAATGGTGCTGATG
TTTTGGTCTCTCGTGCTTTAAGTCAAAGTGTGGTCGTTGCTGAAGGTCTTAATCTTGAGTTGGTAAAAACAGCTCGTGGG
CGTTTAGATCATCATTACCATGAGGGTGGCCATAAGCTGAGCCATCTTTACCCAAACGTAAAAGAGACATTAGAGTCGCT
ATATCAGGAAGGGTATACGCTAGCGCTTGTGACGAATAAGCCCGCGCAATTTGTTCCTGAATTATTAAAACAACATCAAC
TTGAACATTTATTTGTTGATGTTATTGGTGGCGATACGTTTGTCGAGAAGAAGCCTAATCCATTCGCTTTAAATTGGTTA
CTCGATAAGCATCATCTTTTGCCGACGCAAATGTTGATGGTGGGTGATTCTCAAAATGATATTCAAGCCGCTAAAGCCGC
TGGTTGTTATAGTTTCGGATTAACTTATGGTTATAACCATGGGGAACCTATTGCAGACAGTGATCCTGATGTTGTGAGTG
ATGAATTTAATCATTTATTAGCCGTGATGAGTGTGGCAAGATAG

Upstream 100 bases:

>100_bases
TGTTTGTGGCAGGCTCTGCAATCTTTAATCAACCAAATTATAAAGAAGTGATAGATGAAATGCGTGCTGAACTCGCTAAA
GTGAAAGGTAATTAAGAGAC

Downstream 100 bases:

>100_bases
TATTCGTTAATAAGTACACGGATTAACGTAATTCGGCATTATTAATATCGATAGAATAACCATTAAATGATCCGATAACG
GGTCATTTAATTTTTACAGT

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP; PGPase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MFENIKLIAFDLDGTLLDSVPDLARAVDLAMQDLDYPGVTLEQASHWIGNGADVLVSRALSQSVVVAEGLNLELVKTARG
RLDHHYHEGGHKLSHLYPNVKETLESLYQEGYTLALVTNKPAQFVPELLKQHQLEHLFVDVIGGDTFVEKKPNPFALNWL
LDKHHLLPTQMLMVGDSQNDIQAAKAAGCYSFGLTYGYNHGEPIADSDPDVVSDEFNHLLAVMSVAR

Sequences:

>Translated_227_residues
MFENIKLIAFDLDGTLLDSVPDLARAVDLAMQDLDYPGVTLEQASHWIGNGADVLVSRALSQSVVVAEGLNLELVKTARG
RLDHHYHEGGHKLSHLYPNVKETLESLYQEGYTLALVTNKPAQFVPELLKQHQLEHLFVDVIGGDTFVEKKPNPFALNWL
LDKHHLLPTQMLMVGDSQNDIQAAKAAGCYSFGLTYGYNHGEPIADSDPDVVSDEFNHLLAVMSVAR
>Mature_227_residues
MFENIKLIAFDLDGTLLDSVPDLARAVDLAMQDLDYPGVTLEQASHWIGNGADVLVSRALSQSVVVAEGLNLELVKTARG
RLDHHYHEGGHKLSHLYPNVKETLESLYQEGYTLALVTNKPAQFVPELLKQHQLEHLFVDVIGGDTFVEKKPNPFALNWL
LDKHHLLPTQMLMVGDSQNDIQAAKAAGCYSFGLTYGYNHGEPIADSDPDVVSDEFNHLLAVMSVAR

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=242, Percent_Identity=45.0413223140496, Blast_Score=188, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR005833
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 25099; Mature: 25099

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFENIKLIAFDLDGTLLDSVPDLARAVDLAMQDLDYPGVTLEQASHWIGNGADVLVSRAL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHH
SQSVVVAEGLNLELVKTARGRLDHHYHEGGHKLSHLYPNVKETLESLYQEGYTLALVTNK
HCCEEEECCCCEEEHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHCCEEEEEEECC
PAQFVPELLKQHQLEHLFVDVIGGDTFVEKKPNPFALNWLLDKHHLLPTQMLMVGDSQND
CHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCHHEEEECCCCCH
IQAAKAAGCYSFGLTYGYNHGEPIADSDPDVVSDEFNHLLAVMSVAR
HHHHHHCCHHHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFENIKLIAFDLDGTLLDSVPDLARAVDLAMQDLDYPGVTLEQASHWIGNGADVLVSRAL
CCCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCHHHHHHHHH
SQSVVVAEGLNLELVKTARGRLDHHYHEGGHKLSHLYPNVKETLESLYQEGYTLALVTNK
HCCEEEECCCCEEEHHHHHHHHHHHHHCCCCCHHHHCCCHHHHHHHHHHCCEEEEEEECC
PAQFVPELLKQHQLEHLFVDVIGGDTFVEKKPNPFALNWLLDKHHLLPTQMLMVGDSQND
CHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCCCHHEEEECCCCCH
IQAAKAAGCYSFGLTYGYNHGEPIADSDPDVVSDEFNHLLAVMSVAR
HHHHHHCCHHHHCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA