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The map label for this gene is parE [H]

Identifier: 209696078

GI number: 209696078

Start: 2888923

End: 2890803

Strand: Reverse

Name: parE [H]

Synonym: VSAL_I2670

Alternate gene names: 209696078

Gene position: 2890803-2888923 (Counterclockwise)

Preceding gene: 209696079

Following gene: 209696077

Centisome position: 86.94

GC content: 43.38

Gene sequence:

>1881_bases
ATGACTGAACAATATAATGCAGGAGCCATTGAGGTTCTAAATGGCCTTGAGCCCGTTCGTCGCCGTCCTGGTATGTATAC
CGACACCTCTCGCCCAAACCATTTAGGGCAAGAAGTTATAGATAACAGTGTCGATGAAGCCTTAGCCGGACACGCGAAAA
AAGTTGAAGTAATTTTGCATGCAGATCAATCGCTTGAAGTGATTGATGATGGCCGTGGTATGCCTGTAGATATCCATCCA
GTAGAGAAAGTCTCTGGTGTGGAGCTTATTTTATGTAAGTTGCACGCCGGTGGTAAATTCTCAGGTAAAAACTACGAATT
CTCCGGTGGTTTGCATGGGGTTGGTATCTCGGTCGTAAATGCGTTATCAAAACGTGTTGAAGTGACCGTTCGTCGTGATG
GCGAAATTTATGAAATTGCGTTTGAGCATGGTGAAAAAGTATCAGACTTAACTGTCGTTGGTACTTGTGGTCGACGTAAC
CGTGGTACCAGTGTGCATTTTTGGCCGGATACCAGTTACTTTGATTCACCTAAATTTTCGGTTTCTCGCCTTGTTAATAA
TTTAAAAGCCAAAGCGGTATTATGCCCAGGTTTAGAAATTGTTTTTAAAGACAAAGTAAACGATAAAGAATACCAATGGT
GTTATGAAGACGGTTTAAAAGATTATCTTTTAGAAGGTGTCGCGGGTTATACCCTATTACCTGAAATTCCATTCACTGGT
GCATTTTTAGGTAAAGGCGAGGCGGCTGATTGGGCGGTTACGTGGTTACCTGAAGGTGGAGAATTAGTTTCTGAAAGTTA
CGTTAACCTTATCCCTACTGCGCAAGGCGGTACACACGTAAATGGTATGCGCCAAGGACTTCTTGATGCAATGCGTGAGT
TTTGTGAATTCCGTAACTTGCTCCCACGCGGGGTTAAATTAACCGCTGATGATATTTGGGATCGTTGTGCTTACGTCTTG
TCGGTTAAGATGCAAGATCCACAGTTCGCGGGTCAAACCAAAGAACGTTTATCATCGAGACAATGTGCTGCGTTTATATC
TGGCGTACTTAAAGATGCTTTCAGCTTATGGTTGAACGAAAAACCACAAATCGCAGAACTTATCGCCGAAGCGTGTATTG
CTAATGCCCATCGTCGTATGCGTGCAGCGAAAAAAGTCGTTCGTAAGAAAATTGCTTCTGGTCCTACGTTACCAGGAAAA
TTGACCGATTGTTCAATTCAAGATTTGGCTCGTACTGAACTTTTCTTAGTGGAAGGGGACTCGGCGGGGGGCTCAGCTAA
GCAAGCGCGTGACCGTGAATTCCAAGCGGTAATGCCATTACGTGGTAAAATTCTTAATACGTGGGAAGTCTCGGCGGATC
AAGTGTTAGCCTCTCAAGAGGTACACAATATTTCAGTCGCGTTAGGTATTGATCCTGATTCGGACGATTTATCGGGATTA
CGTTACGGTAAAGTTTGTATCTTAGCCGATGCCGATTCAGATGGGCTTCATATCGCAACATTGTTGTGTGCTTTATATCT
AAAACACTTCACGTCTTTAGTTCGTGCTGGTCACGTCTATGTGGCAATGCCGCCACTGTATCGTATCGACTGCGGTAAAG
AAGTTTTCTACGCCCTTGATGAGGCAGAGAAAGAAGGTGTGTTAGAGCGTTTAAGTAATAAACGTGCAAAAATTAATGTT
CAACGATTTAAAGGATTGGGCGAGATGAACCCACTTCAACTTCGTGAAACAACCATGGATCCAAACACCCGTCGTCTTGT
TCAACTGACCATTGATGATGATGAAAAAACTAATGAAATGATGGATATGCTTTTAGGTAAAAAACGTGCTGACGATCGTC
GAGCTTGGTTACAATCTAATGGCGATCTGGCAGAGGTATAA

Upstream 100 bases:

>100_bases
TAATACAAAGCCACATATTTAGAGAGGAATAGGGGCGATTGCTTGACTTTCCTTTCTGCCTTTCAGACTATGTAACAAAT
TTTAAGAAGCCTTCCGAGAT

Downstream 100 bases:

>100_bases
CAAATGAGTGCAGAAATGTCCTTAGAAGGTGTTGAACAGCTGCCGCTGCGCAAGTTTACAGAAGACGCTTATCTAAATTA
CTCCATGTACGTAATCATGG

Product: DNA topoisomerase IV subunit B

Products: NA

Alternate protein names: Topoisomerase IV subunit B [H]

Number of amino acids: Translated: 626; Mature: 625

Protein sequence:

>626_residues
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHAKKVEVILHADQSLEVIDDGRGMPVDIHP
VEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVNALSKRVEVTVRRDGEIYEIAFEHGEKVSDLTVVGTCGRRN
RGTSVHFWPDTSYFDSPKFSVSRLVNNLKAKAVLCPGLEIVFKDKVNDKEYQWCYEDGLKDYLLEGVAGYTLLPEIPFTG
AFLGKGEAADWAVTWLPEGGELVSESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNLLPRGVKLTADDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFISGVLKDAFSLWLNEKPQIAELIAEACIANAHRRMRAAKKVVRKKIASGPTLPGK
LTDCSIQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHNISVALGIDPDSDDLSGL
RYGKVCILADADSDGLHIATLLCALYLKHFTSLVRAGHVYVAMPPLYRIDCGKEVFYALDEAEKEGVLERLSNKRAKINV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEKTNEMMDMLLGKKRADDRRAWLQSNGDLAEV

Sequences:

>Translated_626_residues
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHAKKVEVILHADQSLEVIDDGRGMPVDIHP
VEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVNALSKRVEVTVRRDGEIYEIAFEHGEKVSDLTVVGTCGRRN
RGTSVHFWPDTSYFDSPKFSVSRLVNNLKAKAVLCPGLEIVFKDKVNDKEYQWCYEDGLKDYLLEGVAGYTLLPEIPFTG
AFLGKGEAADWAVTWLPEGGELVSESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNLLPRGVKLTADDIWDRCAYVL
SVKMQDPQFAGQTKERLSSRQCAAFISGVLKDAFSLWLNEKPQIAELIAEACIANAHRRMRAAKKVVRKKIASGPTLPGK
LTDCSIQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHNISVALGIDPDSDDLSGL
RYGKVCILADADSDGLHIATLLCALYLKHFTSLVRAGHVYVAMPPLYRIDCGKEVFYALDEAEKEGVLERLSNKRAKINV
QRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEKTNEMMDMLLGKKRADDRRAWLQSNGDLAEV
>Mature_625_residues
TEQYNAGAIEVLNGLEPVRRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHAKKVEVILHADQSLEVIDDGRGMPVDIHPV
EKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVNALSKRVEVTVRRDGEIYEIAFEHGEKVSDLTVVGTCGRRNR
GTSVHFWPDTSYFDSPKFSVSRLVNNLKAKAVLCPGLEIVFKDKVNDKEYQWCYEDGLKDYLLEGVAGYTLLPEIPFTGA
FLGKGEAADWAVTWLPEGGELVSESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNLLPRGVKLTADDIWDRCAYVLS
VKMQDPQFAGQTKERLSSRQCAAFISGVLKDAFSLWLNEKPQIAELIAEACIANAHRRMRAAKKVVRKKIASGPTLPGKL
TDCSIQDLARTELFLVEGDSAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHNISVALGIDPDSDDLSGLR
YGKVCILADADSDGLHIATLLCALYLKHFTSLVRAGHVYVAMPPLYRIDCGKEVFYALDEAEKEGVLERLSNKRAKINVQ
RFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEKTNEMMDMLLGKKRADDRRAWLQSNGDLAEV

Specific function: Topoisomerase IV is essential for chromosome segregation. It relaxes supercoiled DNA. Performs the decatenation events required during the replication of a circular DNA molecule [H]

COG id: COG0187

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), B subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type II topoisomerase family [H]

Homologues:

Organism=Homo sapiens, GI19913406, Length=592, Percent_Identity=25.3378378378378, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI19913408, Length=591, Percent_Identity=25.2115059221658, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1789408, Length=628, Percent_Identity=80.0955414012739, Blast_Score=1071, Evalue=0.0,
Organism=Escherichia coli, GI48994957, Length=552, Percent_Identity=39.6739130434783, Blast_Score=374, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI212645845, Length=597, Percent_Identity=25.1256281407035, Blast_Score=126, Evalue=3e-29,
Organism=Caenorhabditis elegans, GI17535065, Length=641, Percent_Identity=24.4929797191888, Blast_Score=123, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI212645657, Length=229, Percent_Identity=27.9475982532751, Blast_Score=73, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324241, Length=623, Percent_Identity=25.2006420545746, Blast_Score=135, Evalue=2e-32,
Organism=Drosophila melanogaster, GI17136538, Length=586, Percent_Identity=24.2320819112628, Blast_Score=123, Evalue=5e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR020568
- InterPro:   IPR014721
- InterPro:   IPR001241
- InterPro:   IPR013759
- InterPro:   IPR002288
- InterPro:   IPR013506
- InterPro:   IPR013760
- InterPro:   IPR018522
- InterPro:   IPR005737 [H]

Pfam domain/function: PF00204 DNA_gyraseB; PF00986 DNA_gyraseB_C; PF02518 HATPase_c [H]

EC number: 5.99.1.-

Molecular weight: Translated: 69268; Mature: 69137

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00177 TOPOISOMERASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEQYNAGAIEVLNGLEPVRRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHAKKVEVILH
CCCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
ADQSLEVIDDGRGMPVDIHPVEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVN
CCCCCEEEECCCCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVTVRRDGEIYEIAFEHGEKVSDLTVVGTCGRRNRGTSVHFWPDTSYFDSPKFS
HHHHHEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCHH
VSRLVNNLKAKAVLCPGLEIVFKDKVNDKEYQWCYEDGLKDYLLEGVAGYTLLPEIPFTG
HHHHHHHHHCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
AFLGKGEAADWAVTWLPEGGELVSESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNL
EECCCCCCCCEEEEEECCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRGVKLTADDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFISGVLKDAFSLWLNE
CCCCCEEEHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQIAELIAEACIANAHRRMRAAKKVVRKKIASGPTLPGKLTDCSIQDLARTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHNISVALGIDPDSDDLSGL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCCCCC
RYGKVCILADADSDGLHIATLLCALYLKHFTSLVRAGHVYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHHHHHH
EAEKEGVLERLSNKRAKINVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEKTNEM
HHHHHHHHHHHCCCHHEEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHH
MDMLLGKKRADDRRAWLQSNGDLAEV
HHHHHCCCCCCHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TEQYNAGAIEVLNGLEPVRRRPGMYTDTSRPNHLGQEVIDNSVDEALAGHAKKVEVILH
CCCCCCCHHHHHHCCHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEEE
ADQSLEVIDDGRGMPVDIHPVEKVSGVELILCKLHAGGKFSGKNYEFSGGLHGVGISVVN
CCCCCEEEECCCCCCEECCCCCCCCCCEEEEEEECCCCCCCCCCEEECCCCCHHHHHHHH
ALSKRVEVTVRRDGEIYEIAFEHGEKVSDLTVVGTCGRRNRGTSVHFWPDTSYFDSPKFS
HHHHHEEEEEECCCCEEEEEECCCCCCCCEEEEECCCCCCCCCEEEECCCCCCCCCCCHH
VSRLVNNLKAKAVLCPGLEIVFKDKVNDKEYQWCYEDGLKDYLLEGVAGYTLLPEIPFTG
HHHHHHHHHCCEEECCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEECCCCCCCC
AFLGKGEAADWAVTWLPEGGELVSESYVNLIPTAQGGTHVNGMRQGLLDAMREFCEFRNL
EECCCCCCCCEEEEEECCCCHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHH
LPRGVKLTADDIWDRCAYVLSVKMQDPQFAGQTKERLSSRQCAAFISGVLKDAFSLWLNE
CCCCCEEEHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
KPQIAELIAEACIANAHRRMRAAKKVVRKKIASGPTLPGKLTDCSIQDLARTELFLVEGD
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCEEEEEEECC
SAGGSAKQARDREFQAVMPLRGKILNTWEVSADQVLASQEVHNISVALGIDPDSDDLSGL
CCCCCHHHHHCCCHHEECCCCCCEEEEECCCHHHHHHHCCCCEEEEEEECCCCCCCCCCC
RYGKVCILADADSDGLHIATLLCALYLKHFTSLVRAGHVYVAMPPLYRIDCGKEVFYALD
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEEECCCHHHHHHHH
EAEKEGVLERLSNKRAKINVQRFKGLGEMNPLQLRETTMDPNTRRLVQLTIDDDEKTNEM
HHHHHHHHHHHCCCHHEEEHHHHCCCCCCCCCEEEECCCCCCCEEEEEEEECCCHHHHHH
MDMLLGKKRADDRRAWLQSNGDLAEV
HHHHHCCCCCCHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2170028; 8388096; 9278503; 8227000; 8980775 [H]