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The map label for this gene is sspA [H]

Identifier: 209696067

GI number: 209696067

Start: 2877265

End: 2877930

Strand: Reverse

Name: sspA [H]

Synonym: VSAL_I2659

Alternate gene names: 209696067

Gene position: 2877930-2877265 (Counterclockwise)

Preceding gene: 209696068

Following gene: 209696066

Centisome position: 86.55

GC content: 39.94

Gene sequence:

>666_bases
ATGGCTGTTGCTGCCAACAAACGCTCAGTAATGACGCTTTTTTCAGACGCTTCTGACATGTACAGTCACCAAGTACGCAT
TGTATTGGCTGAGAAAGGAGTAAGTGTTGAAGTTGAATTGGTTGAACCATCCAACTTACCTGCAGAACTTGTAGAGTTAA
ACCCTTACAAAACGGTTCCAACATTAGTTGACCGCGAGCTTGCACTATATAACTCAAACATCATTATGGAATATTTAGAT
GAGCGTTTTCCTCATCCGCCATTGATGCCTGTTTATCCTGTCGCTCGTGGTAATAGTCGTTTGATGATGCACCGTGTTGA
AACTAACTGGTACTCATTGGCTAAGATCATTGCTACTGGTACTTTTGAAGAATCAGAATCAGAATCAGAATCAGAATCAG
AATCAGAAGCGGCACGTGCTAAATTGCGTAATGATTTATTGATGTTAGGTCCTATCTTCGCTGAGTACACTTACTTCATG
AGTGATGAATTTAGCTTAGTTGACTGTTATTTAGCTCCGCTGTTATGGCGTTTACCTTCTATGGGTATTGAGTTATCTGG
TCCGGGTTCTAAAGAGATGAAAGTGTATATGAATCGTGTATTTGAACGAGATTCATTCCTAGCATCTTTAACTGAAATTG
AACGTGAAATGCGTCTAACGAACTAA

Upstream 100 bases:

>100_bases
TGTGTCACTACTTATTTTTATACGAATGTTATAAAAAAATTAGCTGACTGGCACCAGAAAATGGTGCTTTTTGCTCCTTA
GATAAATACCGGAGGGCTCA

Downstream 100 bases:

>100_bases
TGGATATTAGTTCTATGATGCCTCGTCGCCCATATTTATTACGTGCTTTTTATGAATGGATTGTAGATAACGAGTTGACT
CCTCATCTTGTTGTTGATGC

Product: stringent starvation protein A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 221; Mature: 220

Protein sequence:

>221_residues
MAVAANKRSVMTLFSDASDMYSHQVRIVLAEKGVSVEVELVEPSNLPAELVELNPYKTVPTLVDRELALYNSNIIMEYLD
ERFPHPPLMPVYPVARGNSRLMMHRVETNWYSLAKIIATGTFEESESESESESESEAARAKLRNDLLMLGPIFAEYTYFM
SDEFSLVDCYLAPLLWRLPSMGIELSGPGSKEMKVYMNRVFERDSFLASLTEIEREMRLTN

Sequences:

>Translated_221_residues
MAVAANKRSVMTLFSDASDMYSHQVRIVLAEKGVSVEVELVEPSNLPAELVELNPYKTVPTLVDRELALYNSNIIMEYLD
ERFPHPPLMPVYPVARGNSRLMMHRVETNWYSLAKIIATGTFEESESESESESESEAARAKLRNDLLMLGPIFAEYTYFM
SDEFSLVDCYLAPLLWRLPSMGIELSGPGSKEMKVYMNRVFERDSFLASLTEIEREMRLTN
>Mature_220_residues
AVAANKRSVMTLFSDASDMYSHQVRIVLAEKGVSVEVELVEPSNLPAELVELNPYKTVPTLVDRELALYNSNIIMEYLDE
RFPHPPLMPVYPVARGNSRLMMHRVETNWYSLAKIIATGTFEESESESESESESEAARAKLRNDLLMLGPIFAEYTYFMS
DEFSLVDCYLAPLLWRLPSMGIELSGPGSKEMKVYMNRVFERDSFLASLTEIEREMRLTN

Specific function: Forms an equimolar complex with the RNA polymerase holoenzyme (RNAP) but not with the core enzyme [H]

COG id: COG0625

COG function: function code O; Glutathione S-transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GST N-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1789624, Length=219, Percent_Identity=69.8630136986301, Blast_Score=317, Evalue=5e-88,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1982 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010987
- InterPro:   IPR004045
- InterPro:   IPR017933
- InterPro:   IPR004046
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF00043 GST_C; PF02798 GST_N [H]

EC number: NA

Molecular weight: Translated: 25208; Mature: 25077

Theoretical pI: Translated: 4.44; Mature: 4.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVAANKRSVMTLFSDASDMYSHQVRIVLAEKGVSVEVELVEPSNLPAELVELNPYKTVP
CCCCCCCCHHHHHHHCHHHHHHCEEEEEEECCCCEEEEEEECCCCCCHHHEECCCCCCCH
TLVDRELALYNSNIIMEYLDERFPHPPLMPVYPVARGNSRLMMHRVETNWYSLAKIIATG
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHC
TFEESESESESESESEAARAKLRNDLLMLGPIFAEYTYFMSDEFSLVDCYLAPLLWRLPS
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
MGIELSGPGSKEMKVYMNRVFERDSFLASLTEIEREMRLTN
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AVAANKRSVMTLFSDASDMYSHQVRIVLAEKGVSVEVELVEPSNLPAELVELNPYKTVP
CCCCCCCHHHHHHHCHHHHHHCEEEEEEECCCCEEEEEEECCCCCCHHHEECCCCCCCH
TLVDRELALYNSNIIMEYLDERFPHPPLMPVYPVARGNSRLMMHRVETNWYSLAKIIATG
HHHHHHHHHHCCCHHHHHHHHHCCCCCCCEEEEECCCCCEEEEEEECCCHHHHHHHHHHC
TFEESESESESESESEAARAKLRNDLLMLGPIFAEYTYFMSDEFSLVDCYLAPLLWRLPS
CCCCCCCCCHHHHHHHHHHHHHHCCEEEEHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCC
MGIELSGPGSKEMKVYMNRVFERDSFLASLTEIEREMRLTN
CCEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]