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The map label for this gene is hemL

Identifier: 209695989

GI number: 209695989

Start: 2766507

End: 2767802

Strand: Direct

Name: hemL

Synonym: VSAL_I2572

Alternate gene names: 209695989

Gene position: 2766507-2767802 (Clockwise)

Preceding gene: 209695985

Following gene: 209695990

Centisome position: 83.2

GC content: 42.98

Gene sequence:

>1296_bases
ATGACCAAATCAGCAGAATTATTCGCAAAAGCGCAAGAGAAAATCCCTGGTGGCGTAAACTCACCCGTTCGTGCTTTTGC
TGGCGTTGGTGGTTCACCAATTTTTATTGAGCGTGCCGATGGCCCATTAATTTTTGATGCCGATGGTAAAGCTTATATTG
ATTATGTAGGTTCTTGGGGTCCAATGATCCTTGGTCACAATCATGCTGCGATCCGTGAAGCGGTTATCTCTGCGGCACAA
CGCGGACTGAGTTTCGGAGCTCCAACTGAAACTGAAATTAACATGGCTGAATTAGTATCAGAATTAGTTCCATCGATGGA
ACAAGTTCGCATGGTAAGTTCTGGTACTGAAGCAACGATGAGTGCGATTCGCCTTGCTCGTGGCTACACTGGTCGTGACA
AAATCATGAAGTTTGAAGGTTGCTATCACGGTCATGCTGATAGTTTGCTTGTAAAAGCGGGTTCAGGCGCATTAACTCTT
GGCCAACCAAGCTCTCCAGGCGTTCCTGCTGATTTTGCTAAATATACATTAACAGCCACCTTTAATAATTTAGATTCTGT
TCGTGAACTTTTTGCTGCAAATAAAGGCGAGATTTCTTGTATTATCGTGGAACCTGTTGCAGGCAACATGAATTGCATTC
CGCCAGTTGAAGGGTTTCATGAAGGTCTTCGTGAAATTTGTGATGCTGAAGGCGCTCTATTAATTTTTGATGAAGTGATG
ACGGGTTTCCGTGTTGCTGAAAATTGTGCTCAAGGCTATTACAACATTAAGCCTGACTTAACTTGTTTAGGTAAAGTTAT
CGGTGGCGGCATGCCTGTAGGCGCATTTGGTGGCCGCAAAGATATAATGCAATACATTGCACCTACGGGTCCTGTATATC
AAGCAGGTACGCTTTCTGGCAACCCCGTAGCGATGGCAGCAGGTTTTGCTTGCTTGAAAGTTCTTACTGAAGAAGGCAAT
GAAAAAAGACTTGCCACAACAACAAAACAACTTGCTTTAGGCTTTAAAGAATTAGCAAATAAACACGGGCTCCCGATGGT
TGTTAACTATGTTGGTGGAATGTTTGGCTTCTTCTTTACAGACCAAGAGACAATCACAACTTACGAAGATGTGACTAAAT
GTGATATCGAACGATTCAAACGTTTCTTTAATCTAATGTTAGCACACGGTGTTTACCTTGCCCCTTCTGCATTTGAAGCT
GGCTTTACTTCTTTAGCACACGGTCCAAAAGAAATCGAAGCAACATTAGAAGCCGCTGATCGTTGTTTTGCTAAAATAGC
GGCCGAATCAAAATAA

Upstream 100 bases:

>100_bases
TTATAAGATGGGGATTAATACGATCAACTCAAGCATTCTAATTCAATTTAGGTACAATAACGTCCAACTGATCCACTTGA
TTACTATGAGGATATAAGTA

Downstream 100 bases:

>100_bases
ATGTAATCAATTAACCGATAAATGGTCTTCTTAATCAAAGAAGACCATTTTTATTGCCAAAAATAAATGACTAGTACTCC
TAGCCCAATAATAATTAACG

Product: glutamate-1-semialdehyde aminotransferase

Products: NA

Alternate protein names: GSA; Glutamate-1-semialdehyde aminotransferase; GSA-AT

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MTKSAELFAKAQEKIPGGVNSPVRAFAGVGGSPIFIERADGPLIFDADGKAYIDYVGSWGPMILGHNHAAIREAVISAAQ
RGLSFGAPTETEINMAELVSELVPSMEQVRMVSSGTEATMSAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKYTLTATFNNLDSVRELFAANKGEISCIIVEPVAGNMNCIPPVEGFHEGLREICDAEGALLIFDEVM
TGFRVAENCAQGYYNIKPDLTCLGKVIGGGMPVGAFGGRKDIMQYIAPTGPVYQAGTLSGNPVAMAAGFACLKVLTEEGN
EKRLATTTKQLALGFKELANKHGLPMVVNYVGGMFGFFFTDQETITTYEDVTKCDIERFKRFFNLMLAHGVYLAPSAFEA
GFTSLAHGPKEIEATLEAADRCFAKIAAESK

Sequences:

>Translated_431_residues
MTKSAELFAKAQEKIPGGVNSPVRAFAGVGGSPIFIERADGPLIFDADGKAYIDYVGSWGPMILGHNHAAIREAVISAAQ
RGLSFGAPTETEINMAELVSELVPSMEQVRMVSSGTEATMSAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTL
GQPSSPGVPADFAKYTLTATFNNLDSVRELFAANKGEISCIIVEPVAGNMNCIPPVEGFHEGLREICDAEGALLIFDEVM
TGFRVAENCAQGYYNIKPDLTCLGKVIGGGMPVGAFGGRKDIMQYIAPTGPVYQAGTLSGNPVAMAAGFACLKVLTEEGN
EKRLATTTKQLALGFKELANKHGLPMVVNYVGGMFGFFFTDQETITTYEDVTKCDIERFKRFFNLMLAHGVYLAPSAFEA
GFTSLAHGPKEIEATLEAADRCFAKIAAESK
>Mature_430_residues
TKSAELFAKAQEKIPGGVNSPVRAFAGVGGSPIFIERADGPLIFDADGKAYIDYVGSWGPMILGHNHAAIREAVISAAQR
GLSFGAPTETEINMAELVSELVPSMEQVRMVSSGTEATMSAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTLG
QPSSPGVPADFAKYTLTATFNNLDSVRELFAANKGEISCIIVEPVAGNMNCIPPVEGFHEGLREICDAEGALLIFDEVMT
GFRVAENCAQGYYNIKPDLTCLGKVIGGGMPVGAFGGRKDIMQYIAPTGPVYQAGTLSGNPVAMAAGFACLKVLTEEGNE
KRLATTTKQLALGFKELANKHGLPMVVNYVGGMFGFFFTDQETITTYEDVTKCDIERFKRFFNLMLAHGVYLAPSAFEAG
FTSLAHGPKEIEATLEAADRCFAKIAAESK

Specific function: Porphyrin biosynthesis by the C5 pathway; second step. [C]

COG id: COG0001

COG function: function code H; Glutamate-1-semialdehyde aminotransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridoxal-phosphate-dependent aminotransferase family. HemL subfamily

Homologues:

Organism=Homo sapiens, GI4557809, Length=297, Percent_Identity=28.6195286195286, Blast_Score=103, Evalue=4e-22,
Organism=Homo sapiens, GI37574042, Length=351, Percent_Identity=26.7806267806268, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI226442705, Length=349, Percent_Identity=26.3610315186246, Blast_Score=89, Evalue=7e-18,
Organism=Homo sapiens, GI284507298, Length=211, Percent_Identity=30.8056872037915, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI226442709, Length=285, Percent_Identity=27.3684210526316, Blast_Score=78, Evalue=1e-14,
Organism=Escherichia coli, GI1786349, Length=426, Percent_Identity=71.1267605633803, Blast_Score=637, Evalue=0.0,
Organism=Escherichia coli, GI1789016, Length=451, Percent_Identity=27.2727272727273, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788044, Length=333, Percent_Identity=30.03003003003, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI145693181, Length=344, Percent_Identity=27.6162790697674, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1787560, Length=351, Percent_Identity=29.6296296296296, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1789759, Length=327, Percent_Identity=26.605504587156, Blast_Score=108, Evalue=6e-25,
Organism=Escherichia coli, GI1786991, Length=390, Percent_Identity=27.1794871794872, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25144271, Length=300, Percent_Identity=26, Blast_Score=104, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI71992977, Length=392, Percent_Identity=26.0204081632653, Blast_Score=99, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI25144274, Length=143, Percent_Identity=32.1678321678322, Blast_Score=75, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6323470, Length=327, Percent_Identity=28.4403669724771, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324432, Length=326, Percent_Identity=23.6196319018405, Blast_Score=77, Evalue=5e-15,
Organism=Drosophila melanogaster, GI21357415, Length=294, Percent_Identity=26.8707482993197, Blast_Score=97, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GSA_ALISL (B6EL04)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263919.1
- ProteinModelPortal:   B6EL04
- GeneID:   6987825
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2572
- HOGENOM:   HBG725944
- OMA:   DKVIKFE
- ProtClustDB:   PRK00062
- GO:   GO:0005737
- HAMAP:   MF_00375
- InterPro:   IPR004639
- InterPro:   IPR005814
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PANTHER:   PTHR11986
- TIGRFAMs:   TIGR00713

Pfam domain/function: PF00202 Aminotran_3; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =5.4.3.8

Molecular weight: Translated: 45949; Mature: 45818

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS00600 AA_TRANSFER_CLASS_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKSAELFAKAQEKIPGGVNSPVRAFAGVGGSPIFIERADGPLIFDADGKAYIDYVGSWG
CCCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEEECCCCC
PMILGHNHAAIREAVISAAQRGLSFGAPTETEINMAELVSELVPSMEQVRMVSSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKYTLTAT
HHHHHHCCCCCHHHHEEECCCCCCCCCCEEEECCCCEEECCCCCCCCCCCHHHHEEEEEE
FNNLDSVRELFAANKGEISCIIVEPVAGNMNCIPPVEGFHEGLREICDAEGALLIFDEVM
CCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
TGFRVAENCAQGYYNIKPDLTCLGKVIGGGMPVGAFGGRKDIMQYIAPTGPVYQAGTLSG
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGFACLKVLTEEGNEKRLATTTKQLALGFKELANKHGLPMVVNYVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
DQETITTYEDVTKCDIERFKRFFNLMLAHGVYLAPSAFEAGFTSLAHGPKEIEATLEAAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
RCFAKIAAESK
HHHHHHHCCCC
>Mature Secondary Structure 
TKSAELFAKAQEKIPGGVNSPVRAFAGVGGSPIFIERADGPLIFDADGKAYIDYVGSWG
CCHHHHHHHHHHHCCCCCCCHHHHHHCCCCCEEEEEECCCCEEECCCCCEEEEECCCCC
PMILGHNHAAIREAVISAAQRGLSFGAPTETEINMAELVSELVPSMEQVRMVSSGTEATM
CEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHCCHHHHH
SAIRLARGYTGRDKIMKFEGCYHGHADSLLVKAGSGALTLGQPSSPGVPADFAKYTLTAT
HHHHHHCCCCCHHHHEEECCCCCCCCCCEEEECCCCEEECCCCCCCCCCCHHHHEEEEEE
FNNLDSVRELFAANKGEISCIIVEPVAGNMNCIPPVEGFHEGLREICDAEGALLIFDEVM
CCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEHHHHH
TGFRVAENCAQGYYNIKPDLTCLGKVIGGGMPVGAFGGRKDIMQYIAPTGPVYQAGTLSG
HHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCCEECCCCCC
NPVAMAAGFACLKVLTEEGNEKRLATTTKQLALGFKELANKHGLPMVVNYVGGMFGFFFT
CCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCC
DQETITTYEDVTKCDIERFKRFFNLMLAHGVYLAPSAFEAGFTSLAHGPKEIEATLEAAD
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEECCCHHHHHHHHHHCCHHHHHHHHHHHH
RCFAKIAAESK
HHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA