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The map label for this gene is ygfA [H]

Identifier: 209695959

GI number: 209695959

Start: 2721911

End: 2722492

Strand: Direct

Name: ygfA [H]

Synonym: VSAL_I2541

Alternate gene names: 209695959

Gene position: 2721911-2722492 (Clockwise)

Preceding gene: 209695958

Following gene: 209695960

Centisome position: 81.86

GC content: 39.86

Gene sequence:

>582_bases
ATGCAATCTCCTCGACACCTTCGACAAGACATCCGTCAACGTCGTAAAAACCTCTCTCAGCAATATCAACATCAAGCCGC
TTACGATCTCGTACAGCAATGCTCCACTGATCTTTCAATACAAAAAGCTCAATCTATTGCTATTTACCTTTCAGCTGATG
GCGAAATAGGTACTCACTTATTAATTGAGTGGCTATGGGAGCAAGGAAAAAACGTTTTCTTACCGGTAATCCATCCTTTT
TCTAAAGGCTACCTACTATTTCTTGAATATGACCGCACGACACCTCTTATCGTAAATAAATACCAAATACGAGAACCCAA
GTTAGATAAAACTAAAGTCATTCCTATCTCTGAATTGGATCTTATCTTAACTCCTCTAGTTGCTTTTGATCACACAGGAA
ACCGGTTAGGTATGGGGGGCGGTTATTACGACCGTACACTTAAGAACTGGCATCAATATCAACAAGGTCCTATGCCTATA
GGTATTGCTCACTCTTGCCAACAAGTCGATATTTTACCTGTTGAATCTTGGGATGTTCCTCTTCCTCGTATCATTACCTC
TGATAAAATATGGAAATGGTAA

Upstream 100 bases:

>100_bases
ATGCTCGGCTTGTACTGAGAAGCCTACGGTAAACTTTGCTGATCCGCCTTGAACCTAGGGTTCAAGGGCTACGTTTCACT
ACGGCACTTTGGGGTATTCC

Downstream 100 bases:

>100_bases
AAAACAACCGAAAACGTTTGCTTTAAGTATCTTCACATCACTTTTTTACTCTTTCTAGACTTATTTTTTCTGACGAAGAA
AAATCTGTACGTTATAATCA

Product: 5-formyltetrahydrofolate cyclo-ligase

Products: ADP; phosphate; 5,10-methenyltetrahydrofolate

Alternate protein names: NA

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MQSPRHLRQDIRQRRKNLSQQYQHQAAYDLVQQCSTDLSIQKAQSIAIYLSADGEIGTHLLIEWLWEQGKNVFLPVIHPF
SKGYLLFLEYDRTTPLIVNKYQIREPKLDKTKVIPISELDLILTPLVAFDHTGNRLGMGGGYYDRTLKNWHQYQQGPMPI
GIAHSCQQVDILPVESWDVPLPRIITSDKIWKW

Sequences:

>Translated_193_residues
MQSPRHLRQDIRQRRKNLSQQYQHQAAYDLVQQCSTDLSIQKAQSIAIYLSADGEIGTHLLIEWLWEQGKNVFLPVIHPF
SKGYLLFLEYDRTTPLIVNKYQIREPKLDKTKVIPISELDLILTPLVAFDHTGNRLGMGGGYYDRTLKNWHQYQQGPMPI
GIAHSCQQVDILPVESWDVPLPRIITSDKIWKW
>Mature_193_residues
MQSPRHLRQDIRQRRKNLSQQYQHQAAYDLVQQCSTDLSIQKAQSIAIYLSADGEIGTHLLIEWLWEQGKNVFLPVIHPF
SKGYLLFLEYDRTTPLIVNKYQIREPKLDKTKVIPISELDLILTPLVAFDHTGNRLGMGGGYYDRTLKNWHQYQQGPMPI
GIAHSCQQVDILPVESWDVPLPRIITSDKIWKW

Specific function: Unknown

COG id: COG0212

COG function: function code H; 5-formyltetrahydrofolate cyclo-ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5-formyltetrahydrofolate cyclo-ligase family [H]

Homologues:

Organism=Homo sapiens, GI5453746, Length=192, Percent_Identity=27.6041666666667, Blast_Score=76, Evalue=2e-14,
Organism=Escherichia coli, GI1789278, Length=183, Percent_Identity=50.8196721311475, Blast_Score=201, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17510857, Length=198, Percent_Identity=30.3030303030303, Blast_Score=76, Evalue=9e-15,
Organism=Saccharomyces cerevisiae, GI6321031, Length=214, Percent_Identity=26.1682242990654, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002698 [H]

Pfam domain/function: PF01812 5-FTHF_cyc-lig [H]

EC number: 6.3.3.2

Molecular weight: Translated: 22463; Mature: 22463

Theoretical pI: Translated: 8.64; Mature: 8.64

Prosite motif: PS00591 GLYCOSYL_HYDROL_F10

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQSPRHLRQDIRQRRKNLSQQYQHQAAYDLVQQCSTDLSIQKAQSIAIYLSADGEIGTHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCHHHH
LIEWLWEQGKNVFLPVIHPFSKGYLLFLEYDRTTPLIVNKYQIREPKLDKTKVIPISELD
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEECCHHHHH
LILTPLVAFDHTGNRLGMGGGYYDRTLKNWHQYQQGPMPIGIAHSCQQVDILPVESWDVP
HHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
LPRIITSDKIWKW
CCCEECCCCCCCC
>Mature Secondary Structure
MQSPRHLRQDIRQRRKNLSQQYQHQAAYDLVQQCSTDLSIQKAQSIAIYLSADGEIGTHL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEECCCEEEEEEECCCCCHHHH
LIEWLWEQGKNVFLPVIHPFSKGYLLFLEYDRTTPLIVNKYQIREPKLDKTKVIPISELD
HHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCEEEEEEECCCCCCCCEEECCHHHHH
LILTPLVAFDHTGNRLGMGGGYYDRTLKNWHQYQQGPMPIGIAHSCQQVDILPVESWDVP
HHHHHHHHHCCCCCEECCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCCC
LPRIITSDKIWKW
CCCEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 5-formyltetrahydrofolate

Specific reaction: ATP + 5-formyltetrahydrofolate = ADP + phosphate + 5,10-methenyltetrahydrofolate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]