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The map label for this gene is yafJ [H]

Identifier: 209695817

GI number: 209695817

Start: 2561138

End: 2561965

Strand: Reverse

Name: yafJ [H]

Synonym: VSAL_I2392

Alternate gene names: 209695817

Gene position: 2561965-2561138 (Counterclockwise)

Preceding gene: 209695818

Following gene: 209695814

Centisome position: 77.05

GC content: 40.82

Gene sequence:

>828_bases
ATGTGTGAATTGCTCGGAATGAGTGCAAATATACCAACGGATATTTGTTTTAGTTTTAAAGGTTTGGTTCAACGTGGTGG
CAATACCGGTCCGCATCGTGATGGCTGGGGGATTACTTTTTATGAAGGGAAAGGATTTCGTACATTTAAAGACCCTAAAC
CAAGTTGTCATTCTAAAATTGCAGAATTGGTTCAAGATTACCCAATAAAAAGTAATGCAGTAGTGAGTCATATTCGACAA
GCTAACCGAGGGGAGGTGAATTTAGAAAATACACATCCTTTTACGCGTGAATTATGGGGTCGATACTGGACTTTTGCACA
TAATGGGCAGCTTACTGGGTTCGATGAATTATCAACTGGGCGTTTTCGTCCTGTCGGTGAAACAGATAGTGAACGTGCTT
TTTGTTGGTTATTAAATCAGCTTGAAGAAAAGTATCCAGAACCCCCCCAAGATATGATGCTGATGTTTGAATATATTTCA
GAATGCAGCGATCAACTAAGAGCGCTTGGTGTTTTTAATATGCTATTGAGTGATGGTGAATACGTAATGACGTATTGCTC
GAATAATTTGCATTGGATCACTCGACGGGCTCCCTTTGGGCAAGCTTCTTTGATTGATGAAGACGTGACCATCGATTTTC
ATAAAGAAACGACACCAAACGATGTCGTGTCAGTGATTGCAACTCAACCTCTGACTGATAATGAAGAATGGCATAAAATG
AAAGTGGGTGAATATGCTCTGTTTCATTTTGGTGAACTAAGTGCCGGAAATCACGATACGGTGCTTGATATGGCGCCGCC
GCCTTCTGAGTGTCCAGTAAATACATAA

Upstream 100 bases:

>100_bases
CAAGAGATCCACATTAAAATTATTCATATAGTGATTCAACTGATTGAAAAAGAAATGGAAAAATAATCCATTGAATTGCG
AATAAAGGAGTAACGCAAGA

Downstream 100 bases:

>100_bases
TAAAATATAAACGTCATCTTATGGTTTAAGATGACGTTTTTACTAAGTGATATTATTTTACTCTTCTGTTTCTAATCCAA
ATGTCCCTAGCGCATCTCCA

Product: amidotransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 275; Mature: 275

Protein sequence:

>275_residues
MCELLGMSANIPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPKPSCHSKIAELVQDYPIKSNAVVSHIRQ
ANRGEVNLENTHPFTRELWGRYWTFAHNGQLTGFDELSTGRFRPVGETDSERAFCWLLNQLEEKYPEPPQDMMLMFEYIS
ECSDQLRALGVFNMLLSDGEYVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSVIATQPLTDNEEWHKM
KVGEYALFHFGELSAGNHDTVLDMAPPPSECPVNT

Sequences:

>Translated_275_residues
MCELLGMSANIPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPKPSCHSKIAELVQDYPIKSNAVVSHIRQ
ANRGEVNLENTHPFTRELWGRYWTFAHNGQLTGFDELSTGRFRPVGETDSERAFCWLLNQLEEKYPEPPQDMMLMFEYIS
ECSDQLRALGVFNMLLSDGEYVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSVIATQPLTDNEEWHKM
KVGEYALFHFGELSAGNHDTVLDMAPPPSECPVNT
>Mature_275_residues
MCELLGMSANIPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPKPSCHSKIAELVQDYPIKSNAVVSHIRQ
ANRGEVNLENTHPFTRELWGRYWTFAHNGQLTGFDELSTGRFRPVGETDSERAFCWLLNQLEEKYPEPPQDMMLMFEYIS
ECSDQLRALGVFNMLLSDGEYVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSVIATQPLTDNEEWHKM
KVGEYALFHFGELSAGNHDTVLDMAPPPSECPVNT

Specific function: Unknown

COG id: COG0121

COG function: function code R; Predicted glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1786417, Length=252, Percent_Identity=74.6031746031746, Blast_Score=408, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: NA

Molecular weight: Translated: 31237; Mature: 31237

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCELLGMSANIPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPKPSCHSKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHH
AELVQDYPIKSNAVVSHIRQANRGEVNLENTHPFTRELWGRYWTFAHNGQLTGFDELSTG
HHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHEEEEECCCCCCCCCCCCC
RFRPVGETDSERAFCWLLNQLEEKYPEPPQDMMLMFEYISECSDQLRALGVFNMLLSDGE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSVIATQPLTDNEEWHKM
EEEEEECCCEEEEEECCCCCCHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHE
KVGEYALFHFGELSAGNHDTVLDMAPPPSECPVNT
ECCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCC
>Mature Secondary Structure
MCELLGMSANIPTDICFSFKGLVQRGGNTGPHRDGWGITFYEGKGFRTFKDPKPSCHSKI
CCCEECCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCCCCCCCCCCCHHHHHH
AELVQDYPIKSNAVVSHIRQANRGEVNLENTHPFTRELWGRYWTFAHNGQLTGFDELSTG
HHHHHHCCCCCHHHHHHHHHCCCCCEEECCCCCHHHHHHHHHEEEEECCCCCCCCCCCCC
RFRPVGETDSERAFCWLLNQLEEKYPEPPQDMMLMFEYISECSDQLRALGVFNMLLSDGE
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
YVMTYCSNNLHWITRRAPFGQASLIDEDVTIDFHKETTPNDVVSVIATQPLTDNEEWHKM
EEEEEECCCEEEEEECCCCCCHHCCCCCCEEEEECCCCHHHHHHHHHCCCCCCCCHHHHE
KVGEYALFHFGELSAGNHDTVLDMAPPPSECPVNT
ECCCEEEEECCCCCCCCCCEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7596361; 9278503 [H]