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The map label for this gene is cysK [H]

Identifier: 209695782

GI number: 209695782

Start: 2531018

End: 2531986

Strand: Direct

Name: cysK [H]

Synonym: VSAL_I2356

Alternate gene names: 209695782

Gene position: 2531018-2531986 (Clockwise)

Preceding gene: 209695781

Following gene: 209695783

Centisome position: 76.12

GC content: 42.52

Gene sequence:

>969_bases
ATGACAAAGATTTACGAAGATAATTCACAAACGATTGGCAATACCCCTCTTGTACGTTTAAACCGTGTGAGTAATGGTAA
TGTATTAGCAAAAGTAGAAGCACGTAATCCAAGCTTTAGCGTTAAATGCCGTATTGGTGCAAACATGGTTTGGGAAGCAG
AAAAAGCGGGTACATTAAGAGAAGGCATAGAGTTAGTTGAACCAACATCAGGAAATACCGGCATTGCATTGGCTTTTGTT
GCCGCAGCTAAAGGGTACAAACTAACACTCACTATGCCTGCATCAATGAGTTTAGAACGTCGTAAGTTGCTTAAAGCACT
AGGGGCAAACCTTGTTCTTACTGAAGCGGTAAAAGGCATGAATGGCGCCATCGCTAAAGCGGAAGAAATTGTAGCAAGTG
ATCCTAGTAAGTATTTACTTCTTCAACAATTTAATAACCCTGCAAACCCGGCGATTCATGAAAAAACAACCGGCCCTGAA
ATCTGGGAAGCGACTGACGGCAACATTGATGTATTTGTTGCTGGAGTAGGTACTGGAGGCACATTAACAGGCACCAGTCG
TTACTTAAAACATACTCAAAATAAAGCCGTACTGTCTGTTGCTGTTGAGCCTGCAGAATCACCAGTTATTGCCCAAGCAC
TTGCAGGCGAAGAGATAAAACCAGCACCACATAAAATTCAAGGGATCGGCGCTGGGTTTATTCCAGGAAACCTAGATTTA
ACGCTAATTGATCGTGTTGAAGCCGTGACATCAGAAGATTCAATCGAAATGGCTCGTCGTTTAATGGAAGAAGAAGGCAT
TTTAGCAGGCATATCTTCAGGAGCAGCCGTCGTTGCAGCAAACCGCATTGCAGCGCTTCCAGAGTTCGCAAATAAGCAAA
TTGTTGTCATATTACCAAGTTCGGGTGAACGTTACCTAAGTACAGCACTTTTTGCTGGTTTGTTTACAGAACAAGAAAAT
GAGCAATAA

Upstream 100 bases:

>100_bases
GCAACTCTTCGTATTCTAGATAGGTCGAGTTGCTCAAATGAGTGACCAAAATATTTACTTTATAATTAATCACGCTTTAC
GTGAAATGAAGGAACAGATC

Downstream 100 bases:

>100_bases
AACCGCAACATTCATGTGTTGAAATCAATTTTTACGCCAAAAAAGCCCCTTATGGTTTTTTTTTGTTGATTTCATTTCCC
TCCTTAGTAATAATCAAGCC

Product: cysteine synthase A

Products: NA

Alternate protein names: CSase A; O-acetylserine (thiol)-lyase A; OAS-TL A; O-acetylserine sulfhydrylase A; Sulfate starvation-induced protein 5; SSI5 [H]

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTKIYEDNSQTIGNTPLVRLNRVSNGNVLAKVEARNPSFSVKCRIGANMVWEAEKAGTLREGIELVEPTSGNTGIALAFV
AAAKGYKLTLTMPASMSLERRKLLKALGANLVLTEAVKGMNGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPE
IWEATDGNIDVFVAGVGTGGTLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
TLIDRVEAVTSEDSIEMARRLMEEEGILAGISSGAAVVAANRIAALPEFANKQIVVILPSSGERYLSTALFAGLFTEQEN
EQ

Sequences:

>Translated_322_residues
MTKIYEDNSQTIGNTPLVRLNRVSNGNVLAKVEARNPSFSVKCRIGANMVWEAEKAGTLREGIELVEPTSGNTGIALAFV
AAAKGYKLTLTMPASMSLERRKLLKALGANLVLTEAVKGMNGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPE
IWEATDGNIDVFVAGVGTGGTLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
TLIDRVEAVTSEDSIEMARRLMEEEGILAGISSGAAVVAANRIAALPEFANKQIVVILPSSGERYLSTALFAGLFTEQEN
EQ
>Mature_321_residues
TKIYEDNSQTIGNTPLVRLNRVSNGNVLAKVEARNPSFSVKCRIGANMVWEAEKAGTLREGIELVEPTSGNTGIALAFVA
AAKGYKLTLTMPASMSLERRKLLKALGANLVLTEAVKGMNGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPEI
WEATDGNIDVFVAGVGTGGTLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDLT
LIDRVEAVTSEDSIEMARRLMEEEGILAGISSGAAVVAANRIAALPEFANKQIVVILPSSGERYLSTALFAGLFTEQENE
Q

Specific function: Cysteine biosynthesis. [C]

COG id: COG0031

COG function: function code E; Cysteine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cysteine synthase/cystathionine beta- synthase family [H]

Homologues:

Organism=Homo sapiens, GI295821202, Length=317, Percent_Identity=37.5394321766561, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI295821200, Length=317, Percent_Identity=37.5394321766561, Blast_Score=190, Evalue=1e-48,
Organism=Homo sapiens, GI4557415, Length=317, Percent_Identity=37.5394321766561, Blast_Score=190, Evalue=1e-48,
Organism=Escherichia coli, GI1788754, Length=323, Percent_Identity=77.7089783281734, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI2367138, Length=307, Percent_Identity=43.9739413680782, Blast_Score=221, Evalue=5e-59,
Organism=Caenorhabditis elegans, GI17535051, Length=315, Percent_Identity=51.1111111111111, Blast_Score=289, Evalue=2e-78,
Organism=Caenorhabditis elegans, GI17562970, Length=316, Percent_Identity=49.6835443037975, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI115535073, Length=312, Percent_Identity=53.8461538461538, Blast_Score=280, Evalue=6e-76,
Organism=Caenorhabditis elegans, GI32566674, Length=307, Percent_Identity=50.4885993485342, Blast_Score=279, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17561720, Length=316, Percent_Identity=48.7341772151899, Blast_Score=255, Evalue=3e-68,
Organism=Caenorhabditis elegans, GI32566672, Length=226, Percent_Identity=47.787610619469, Blast_Score=208, Evalue=4e-54,
Organism=Caenorhabditis elegans, GI17534315, Length=321, Percent_Identity=40.4984423676012, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI25147552, Length=322, Percent_Identity=36.9565217391304, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17561716, Length=131, Percent_Identity=48.8549618320611, Blast_Score=107, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6321594, Length=312, Percent_Identity=37.1794871794872, Blast_Score=172, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6321449, Length=324, Percent_Identity=34.8765432098765, Blast_Score=130, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24643623, Length=306, Percent_Identity=37.5816993464052, Blast_Score=156, Evalue=1e-38,
Organism=Drosophila melanogaster, GI20129101, Length=306, Percent_Identity=37.5816993464052, Blast_Score=156, Evalue=1e-38,

Paralogues:

None

Copy number: 2820 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001216
- InterPro:   IPR005856
- InterPro:   IPR005859
- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =2.5.1.47 [H]

Molecular weight: Translated: 34091; Mature: 33960

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00901 CYS_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKIYEDNSQTIGNTPLVRLNRVSNGNVLAKVEARNPSFSVKCRIGANMVWEAEKAGTLR
CCEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCEEEECHHCCCHH
EGIELVEPTSGNTGIALAFVAAAKGYKLTLTMPASMSLERRKLLKALGANLVLTEAVKGM
HCCEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEHHHCCC
NGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPEIWEATDGNIDVFVAGVGTGG
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCC
TLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
CCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCE
TLIDRVEAVTSEDSIEMARRLMEEEGILAGISSGAAVVAANRIAALPEFANKQIVVILPS
EEEEHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHCHHHCCCEEEEEECC
SGERYLSTALFAGLFTEQENEQ
CCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TKIYEDNSQTIGNTPLVRLNRVSNGNVLAKVEARNPSFSVKCRIGANMVWEAEKAGTLR
CEEECCCCCCCCCCCEEEEEEECCCCEEEEEECCCCCEEEEEEECCCEEEECHHCCCHH
EGIELVEPTSGNTGIALAFVAAAKGYKLTLTMPASMSLERRKLLKALGANLVLTEAVKGM
HCCEEECCCCCCCCEEEEEEECCCCEEEEEEECCCCCHHHHHHHHHHCCCEEEEHHHCCC
NGAIAKAEEIVASDPSKYLLLQQFNNPANPAIHEKTTGPEIWEATDGNIDVFVAGVGTGG
CCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCCCCCEEECCCCCEEEEEEEECCCC
TLTGTSRYLKHTQNKAVLSVAVEPAESPVIAQALAGEEIKPAPHKIQGIGAGFIPGNLDL
CCCCCHHHHHHCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCEEEECCCCCCCCCCCE
TLIDRVEAVTSEDSIEMARRLMEEEGILAGISSGAAVVAANRIAALPEFANKQIVVILPS
EEEEHHHHHCCCHHHHHHHHHHHHCCCEEECCCCCEEEEHHHHHHCHHHCCCEEEEEECC
SGERYLSTALFAGLFTEQENEQ
CCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]