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The map label for this gene is ligA

Identifier: 209695779

GI number: 209695779

Start: 2526803

End: 2528812

Strand: Reverse

Name: ligA

Synonym: VSAL_I2353

Alternate gene names: 209695779

Gene position: 2528812-2526803 (Counterclockwise)

Preceding gene: 209695780

Following gene: 209695778

Centisome position: 76.05

GC content: 41.54

Gene sequence:

>2010_bases
ATGAGTATTGAAAAAACACTAGATGAGCTTAAAAAACAGCTTAATTATCACGCTTACCGTTATTATGTTGAAGACAGTCC
AGAGCTTCCTGATGCTGAATATGATCGAATGATGCAGCAACTATTGTTGATTGAAAAAGAACACCCAGAGTTCATGACTG
TCGACTCTCCAAGCCAGCGCGTTGGTGGTGAAGCATTAGGTGGTTTTACTCAAGTTCAACATGAGATCCCTATGTTGTCA
TTGGATAATGCATTTAACGATAATGAACTCGACTCTTTTGAAAAACGCATTAATGATCGTCTTATTTCGGATTCGGTTTC
CTTGTTTTGTTGTGAGCCTAAGCTTGATGGTTTAGCGGTAAGCCTTTTGTATGTTGATGGGCTGTTAGTTCAAGCGGGAA
CTCGTGGTGATGGTGCAACAGGCGAAAATATTACAGAGAACGTAAGAACTATTCGTTGCATACCGCTAACGTTACAAGGA
GAAGGATGGCCAACTCGTTTAGAGGTTCGCGGTGAAGTCTTCATGCCTAAAGCCGGGTTTGATGCGTTAAATGAAAGAGC
GCTAAAACGTGCTGAGAAGCCATTTGCTAACCCCCGTAATGCGGCCGCTGGCAGTTTGCGTCAACTTGATTCAAAAATCA
CAGCAACTCGCCCATTGAGCTTTTATGCATATAGTGTAGGAATCATTGAGGGGGGGGAGCTAGAAGGCAGTCATTATCAA
CGGTTTGTTCAGTTAAAAAACTGGGGTTTACCTATGTGTGAAGAGACAAAACAATGCCACTCACTGACCGATGTAAAAGC
ATATTATAAAGACATTCTCGAGCGTCGAGATGCATTGAAATATGAAATTGATGGTGTAGTGATTAAGGTTGATAGTATTT
CTTTGCAAGAGCAACTAGGCTTTGTTGCAAGAGCCCCGCGTTGGGCTATTGCTTATAAATTTCCTGCTCAAGAAGAACTG
ACCGTTTTAAACGATGTTGAATTTCAGGTCGGTCGTACTGGGGCAATAACCCCCGTAGCCAAATTAGAGCCTATTTTTGT
TGGCGGGGTGACGGTGAGTAATGCGACATTACACAATGCCGATGAGATTGCTCGCTTAGGGGTTCATATTGGTGATACTG
TCATTATTCGTCGTGCTGGTGATGTTATCCCTCAGATTGTATCAGTCGTAGAAGCTCGTCGTCCTGAAGACAGTAAGGCG
ATTATCTATCCAACAACATGCCCTGCTTGTGATTCTCAACTGGAACGAATTGAAGGCGAAGTCGTCACGCGTTGTGTCGC
TGGTTTAGTTTGTCCTGCTCAACGTAAAGAAGCGTTAAAACATTTTGTTTCTCGCAAAGCGCTTGATGTAGATGGCTTGG
GAGATAAAGTGGTAGAGCAACTCGTAGATAAAGAAATGGTGGAGACACCGGCTGATTTATTTAAACTGTCTGCAGGTGTA
TTAACAGTCCTCGATCGTATGGGACCAAAGTCAGCGCAGAATGTGGTCAATGCATTAAATAAAGCGAAAGAAACGACGTT
ATCACGTTTCTTATATTCTTTAGGGATCAGAGAAGTTGGAGAGGCGACTGCGGCTAATTTAGCTCTCCATTTTCAAACAT
TAAATGCAATAAGTTCCGCCACATTTGAACAATTAATTGAAGTCAGTGATGTTGGCGATATTGTTGCTAAACATATACTT
GGTTTCTTTAGTGAGCCTCATAATCAAGCTGTGATTGAAAATTTACAACTGATGGGCGTTAATTGGCCGGATATTAAAGC
GCTTGATGAATCGGTTCCTCAGCCACTAGCTGATAAAGTTGTTGTATTAACAGGAACATTATACAAACTAAAACGCAATG
AAGCTAAAGCGGCACTACAAGAATTAGGCGCTAAAGTTGCTGGTAGTGTATCGAAGAATACCGATATTCTTTTTGCTGGA
GAAGCAGCCGGTTCAAAATTAGCCAAAGCAGAAGAACTCGGTGTTGAAATAATGAATGAAGAACAGTTGATTGAAATATT
AAATAATTAA

Upstream 100 bases:

>100_bases
TTGTAATTAATCGTCTATTATTATCTTAATTTTTAAAGGCTCATTCGTGAGTCTTTTTTGTTTGTATACCATCAATAAAA
GAACAAGAAGTTGACCCGTC

Downstream 100 bases:

>100_bases
TAATAAGTCATGCAACTAAAGGAGCCTTAAAGGCTCCTTTTTTTATGTTTTAAATTTACTTTTTGTTTTTATCCACTATT
TAGTGGGATGTGAATAAATG

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 669; Mature: 668

Protein sequence:

>669_residues
MSIEKTLDELKKQLNYHAYRYYVEDSPELPDAEYDRMMQQLLLIEKEHPEFMTVDSPSQRVGGEALGGFTQVQHEIPMLS
LDNAFNDNELDSFEKRINDRLISDSVSLFCCEPKLDGLAVSLLYVDGLLVQAGTRGDGATGENITENVRTIRCIPLTLQG
EGWPTRLEVRGEVFMPKAGFDALNERALKRAEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGIIEGGELEGSHYQ
RFVQLKNWGLPMCEETKQCHSLTDVKAYYKDILERRDALKYEIDGVVIKVDSISLQEQLGFVARAPRWAIAYKFPAQEEL
TVLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNADEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPEDSKA
IIYPTTCPACDSQLERIEGEVVTRCVAGLVCPAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGV
LTVLDRMGPKSAQNVVNALNKAKETTLSRFLYSLGIREVGEATAANLALHFQTLNAISSATFEQLIEVSDVGDIVAKHIL
GFFSEPHNQAVIENLQLMGVNWPDIKALDESVPQPLADKVVVLTGTLYKLKRNEAKAALQELGAKVAGSVSKNTDILFAG
EAAGSKLAKAEELGVEIMNEEQLIEILNN

Sequences:

>Translated_669_residues
MSIEKTLDELKKQLNYHAYRYYVEDSPELPDAEYDRMMQQLLLIEKEHPEFMTVDSPSQRVGGEALGGFTQVQHEIPMLS
LDNAFNDNELDSFEKRINDRLISDSVSLFCCEPKLDGLAVSLLYVDGLLVQAGTRGDGATGENITENVRTIRCIPLTLQG
EGWPTRLEVRGEVFMPKAGFDALNERALKRAEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGIIEGGELEGSHYQ
RFVQLKNWGLPMCEETKQCHSLTDVKAYYKDILERRDALKYEIDGVVIKVDSISLQEQLGFVARAPRWAIAYKFPAQEEL
TVLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNADEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPEDSKA
IIYPTTCPACDSQLERIEGEVVTRCVAGLVCPAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGV
LTVLDRMGPKSAQNVVNALNKAKETTLSRFLYSLGIREVGEATAANLALHFQTLNAISSATFEQLIEVSDVGDIVAKHIL
GFFSEPHNQAVIENLQLMGVNWPDIKALDESVPQPLADKVVVLTGTLYKLKRNEAKAALQELGAKVAGSVSKNTDILFAG
EAAGSKLAKAEELGVEIMNEEQLIEILNN
>Mature_668_residues
SIEKTLDELKKQLNYHAYRYYVEDSPELPDAEYDRMMQQLLLIEKEHPEFMTVDSPSQRVGGEALGGFTQVQHEIPMLSL
DNAFNDNELDSFEKRINDRLISDSVSLFCCEPKLDGLAVSLLYVDGLLVQAGTRGDGATGENITENVRTIRCIPLTLQGE
GWPTRLEVRGEVFMPKAGFDALNERALKRAEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGIIEGGELEGSHYQR
FVQLKNWGLPMCEETKQCHSLTDVKAYYKDILERRDALKYEIDGVVIKVDSISLQEQLGFVARAPRWAIAYKFPAQEELT
VLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNADEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPEDSKAI
IYPTTCPACDSQLERIEGEVVTRCVAGLVCPAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGVL
TVLDRMGPKSAQNVVNALNKAKETTLSRFLYSLGIREVGEATAANLALHFQTLNAISSATFEQLIEVSDVGDIVAKHILG
FFSEPHNQAVIENLQLMGVNWPDIKALDESVPQPLADKVVVLTGTLYKLKRNEAKAALQELGAKVAGSVSKNTDILFAGE
AAGSKLAKAEELGVEIMNEEQLIEILNN

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=61.7339312406577, Blast_Score=873, Evalue=0.0,
Organism=Escherichia coli, GI87082305, Length=586, Percent_Identity=21.160409556314, Blast_Score=99, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_ALISL (B6EJQ6)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263709.1
- GeneID:   6987712
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2353
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- ProtClustDB:   PRK07956
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 73677; Mature: 73546

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 114-114 BINDING 112-112 BINDING 135-135 BINDING 172-172 BINDING 289-289 BINDING 313-313

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEKTLDELKKQLNYHAYRYYVEDSPELPDAEYDRMMQQLLLIEKEHPEFMTVDSPSQR
CCHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHH
VGGEALGGFTQVQHEIPMLSLDNAFNDNELDSFEKRINDRLISDSVSLFCCEPKLDGLAV
CCCHHHCCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEE
SLLYVDGLLVQAGTRGDGATGENITENVRTIRCIPLTLQGEGWPTRLEVRGEVFMPKAGF
HHHHHCCEEEECCCCCCCCCCCCHHHCCEEEEEEEEEEECCCCCEEEEECCCEECCCCCH
DALNERALKRAEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGIIEGGELEGSHYQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHH
RFVQLKNWGLPMCEETKQCHSLTDVKAYYKDILERRDALKYEIDGVVIKVDSISLQEQLG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECCCHHHHHH
FVARAPRWAIAYKFPAQEELTVLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNA
HHHCCCCEEEEEECCCCCCCHHHCCCEEEECCCCCCCCHHHCCEEEECCEEECCCCCCCH
DEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPEDSKAIIYPTTCPACDSQLERIEGE
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
VVTRCVAGLVCPAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGV
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
LTVLDRMGPKSAQNVVNALNKAKETTLSRFLYSLGIREVGEATAANLALHFQTLNAISSA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHH
TFEQLIEVSDVGDIVAKHILGFFSEPHNQAVIENLQLMGVNWPDIKALDESVPQPLADKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHEECCCCCCHHHHHHHCCCCHHCCE
VVLTGTLYKLKRNEAKAALQELGAKVAGSVSKNTDILFAGEAAGSKLAKAEELGVEIMNE
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCHHHCCH
EQLIEILNN
HHHHHHHCC
>Mature Secondary Structure 
SIEKTLDELKKQLNYHAYRYYVEDSPELPDAEYDRMMQQLLLIEKEHPEFMTVDSPSQR
CHHHHHHHHHHHHCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECCCHHH
VGGEALGGFTQVQHEIPMLSLDNAFNDNELDSFEKRINDRLISDSVSLFCCEPKLDGLAV
CCCHHHCCHHHHHHHCCEEEECCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCCEE
SLLYVDGLLVQAGTRGDGATGENITENVRTIRCIPLTLQGEGWPTRLEVRGEVFMPKAGF
HHHHHCCEEEECCCCCCCCCCCCHHHCCEEEEEEEEEEECCCCCEEEEECCCEECCCCCH
DALNERALKRAEKPFANPRNAAAGSLRQLDSKITATRPLSFYAYSVGIIEGGELEGSHYQ
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCHHHH
RFVQLKNWGLPMCEETKQCHSLTDVKAYYKDILERRDALKYEIDGVVIKVDSISLQEQLG
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECEEEEEEECCCHHHHHH
FVARAPRWAIAYKFPAQEELTVLNDVEFQVGRTGAITPVAKLEPIFVGGVTVSNATLHNA
HHHCCCCEEEEEECCCCCCCHHHCCCEEEECCCCCCCCHHHCCEEEECCEEECCCCCCCH
DEIARLGVHIGDTVIIRRAGDVIPQIVSVVEARRPEDSKAIIYPTTCPACDSQLERIEGE
HHHHHHCCCCCCEEEEECHHHHHHHHHHHHHHCCCCCCCEEEEECCCCHHHHHHHHHHHH
VVTRCVAGLVCPAQRKEALKHFVSRKALDVDGLGDKVVEQLVDKEMVETPADLFKLSAGV
HHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHH
LTVLDRMGPKSAQNVVNALNKAKETTLSRFLYSLGIREVGEATAANLALHFQTLNAISSA
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHCCHHHHHHHHHHHHHHHHHHH
TFEQLIEVSDVGDIVAKHILGFFSEPHNQAVIENLQLMGVNWPDIKALDESVPQPLADKV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCHHEECCCCCCHHHHHHHCCCCHHCCE
VVLTGTLYKLKRNEAKAALQELGAKVAGSVSKNTDILFAGEAAGSKLAKAEELGVEIMNE
EEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCHHHHHHHHCHHHCCH
EQLIEILNN
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA