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The map label for this gene is gltX

Identifier: 209695777

GI number: 209695777

Start: 2522708

End: 2524132

Strand: Reverse

Name: gltX

Synonym: VSAL_I2350

Alternate gene names: 209695777

Gene position: 2524132-2522708 (Counterclockwise)

Preceding gene: 209695778

Following gene: 209695772

Centisome position: 75.91

GC content: 40.42

Gene sequence:

>1425_bases
ATGACGGTTAAAACACGTTTTGCTCCAAGCCCAACAGGCTACCTTCACGTTGGTGGTGCACGCACTGCACTTTATTCATG
GCTTTTTGCTAAAAACAAAGGCGGCGAATTTGTACTTCGTATTGAAGACACAGATTTAGAACGTAACTCTCAAGAAGCGG
TTGATGCAATTCTTGAAGGCATGAAGTGGATGGGAATGGAGTGGGACGAAGGTCCATACTACCAATCTAAGCGTTTTGAT
CGTTATAACGAAGTCGTAGATCTATTGCTTTCTGAAGATAAAGCATACAAATGTTATGCTTCAAAAGAATTGCTTGATGA
AATTCGTACAGAGCAAGAAGAAAATAAAGAGATGGCTCGCTACGATGCAAACCATCCTAAAATTGTTGCCGCAAATGCCG
CAGCAAAAGAAGGCGATGCTTCTGTTATTCGCTTCCGTAATCCAAAAGAAGGCAGTGTTGTCTTTGATGACCAAATCCGT
GGTCGTATTGAAATCTCAAACAGCCAATTAGATGACTTGATCATTCGTCGTACTGATGGTGCACCAACGTATAACTTCGT
TGTTGTCGTTGATGACTGGGATATGGGTATTACTCAGGTTATTCGTGGTGAAGATCATATTAATAATACACCTCGTCAAA
TCAATATCTATGAAGCACTTGGTGCTCCAGTACCAATGTTTGCACACTGTGCAATGATCCTTGGTGATGATGGCGCTAAA
TTATCTAAGCGTCATGGCGCGGTATCGGTAATGCAGTATCGTGATGAAGGCTATCTACCTAATGCACTAAATAACTATTT
AGTTCGTCTTGGTTGGTCTCATGGCGATCAAGAAATCTTCTCTCAAGAAGAAATGATTAACTTATTTAGCTTAGATGCAG
TAAGTAAATCAGCGTCGGCGTTTAATACTGATAAACTTCGTTGGTTGAACAATCATTACATCAAAACATCTGAACCAGAA
TATGTAGCTAACTACCTACAATGGCATTTAGATCAAAAAGAAATCTCTTTAGACAATGGTCCTGCAATTACTGACGTTAT
TACGTTAGTTAGTGAGCGTTGTAATACATTGATTGAGCTGGCTGACCAATCTCGTTATTTCTACGAAGACTTCGAAGCAT
TTGATGCTGGTGCAGCTAAAAAACATCTACGTGGTGTTGCAAAAGGTCCTTTAGAATTAGCGCTTGCTAAAATTGAAGCT
CTACAAGAGTGGACAACAGAGAATCTGCATAACGTTATTGAAGAAGTTTGTGCAGAGCTAGAAATCGGTATGGGTAAAAT
TGGTATGCCTTTGCGTGTAGCCGTTACTGGCGGTGGTCAATCACCATCAGTTGACGCTGTAATGCAGTTAATCGGTAAAG
AACGAGTAGTTACTCGAATTAAGATGGCGTTAGCATTTATTGCTGAACGTGAAGCGAACGCTTAA

Upstream 100 bases:

>100_bases
CCTCACTCAATCATTGTAGATTTACCTTATGGTGCTAAAATAGCGCATAAATTACATGGTGCTGATAGATTCAGCTCAAC
CTCTTCAAAAGGTAATATCG

Downstream 100 bases:

>100_bases
TTCAAATACGATTAAATTAAAAAACCGCTGATTTCAGCGGTTTTTTTTGTGCTAAAGAATGCTTTTAATTGTCCCTTCCG
CTGCTTTACACAGTGTTGCA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNKGGEFVLRIEDTDLERNSQEAVDAILEGMKWMGMEWDEGPYYQSKRFD
RYNEVVDLLLSEDKAYKCYASKELLDEIRTEQEENKEMARYDANHPKIVAANAAAKEGDASVIRFRNPKEGSVVFDDQIR
GRIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLDAVSKSASAFNTDKLRWLNNHYIKTSEPE
YVANYLQWHLDQKEISLDNGPAITDVITLVSERCNTLIELADQSRYFYEDFEAFDAGAAKKHLRGVAKGPLELALAKIEA
LQEWTTENLHNVIEEVCAELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVTRIKMALAFIAEREANA

Sequences:

>Translated_474_residues
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNKGGEFVLRIEDTDLERNSQEAVDAILEGMKWMGMEWDEGPYYQSKRFD
RYNEVVDLLLSEDKAYKCYASKELLDEIRTEQEENKEMARYDANHPKIVAANAAAKEGDASVIRFRNPKEGSVVFDDQIR
GRIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLDAVSKSASAFNTDKLRWLNNHYIKTSEPE
YVANYLQWHLDQKEISLDNGPAITDVITLVSERCNTLIELADQSRYFYEDFEAFDAGAAKKHLRGVAKGPLELALAKIEA
LQEWTTENLHNVIEEVCAELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVTRIKMALAFIAEREANA
>Mature_473_residues
TVKTRFAPSPTGYLHVGGARTALYSWLFAKNKGGEFVLRIEDTDLERNSQEAVDAILEGMKWMGMEWDEGPYYQSKRFDR
YNEVVDLLLSEDKAYKCYASKELLDEIRTEQEENKEMARYDANHPKIVAANAAAKEGDASVIRFRNPKEGSVVFDDQIRG
RIEISNSQLDDLIIRRTDGAPTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAKL
SKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLDAVSKSASAFNTDKLRWLNNHYIKTSEPEY
VANYLQWHLDQKEISLDNGPAITDVITLVSERCNTLIELADQSRYFYEDFEAFDAGAAKKHLRGVAKGPLELALAKIEAL
QEWTTENLHNVIEEVCAELEIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVTRIKMALAFIAEREANA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=328, Percent_Identity=35.0609756097561, Blast_Score=206, Evalue=5e-53,
Organism=Homo sapiens, GI62241042, Length=340, Percent_Identity=24.4117647058824, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1788743, Length=471, Percent_Identity=67.515923566879, Blast_Score=681, Evalue=0.0,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=35.2226720647773, Blast_Score=138, Evalue=1e-33,
Organism=Escherichia coli, GI1786895, Length=111, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=3e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=482, Percent_Identity=29.045643153527, Blast_Score=196, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6324540, Length=501, Percent_Identity=32.3353293413174, Blast_Score=244, Evalue=3e-65,
Organism=Saccharomyces cerevisiae, GI6321192, Length=215, Percent_Identity=27.4418604651163, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324742, Length=125, Percent_Identity=31.2, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24665321, Length=492, Percent_Identity=32.1138211382114, Blast_Score=233, Evalue=2e-61,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_ALISL (B6EJQ3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263707.1
- GeneID:   6987711
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2350
- HOGENOM:   HBG628189
- OMA:   NEIFAKR
- ProtClustDB:   PRK01406
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 53360; Mature: 53229

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 243-243

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKTRFAPSPTGYLHVGGARTALYSWLFAKNKGGEFVLRIEDTDLERNSQEAVDAILEG
CCCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
MKWMGMEWDEGPYYQSKRFDRYNEVVDLLLSEDKAYKCYASKELLDEIRTEQEENKEMAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
YDANHPKIVAANAAAKEGDASVIRFRNPKEGSVVFDDQIRGRIEISNSQLDDLIIRRTDG
HCCCCCEEEEECCCCCCCCCCEEEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCC
APTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
CCCEEEEEEEECCCCCHHHHHCCCHHCCCCCCEEEEHHHHCCCCCHHHHHHHHCCCCCCH
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLDAVSKSASA
HHHHCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLRWLNNHYIKTSEPEYVANYLQWHLDQKEISLDNGPAITDVITLVSERCNTLIEL
CCCHHHHECCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ADQSRYFYEDFEAFDAGAAKKHLRGVAKGPLELALAKIEALQEWTTENLHNVIEEVCAEL
HHCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVTRIKMALAFIAEREANA
HCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVKTRFAPSPTGYLHVGGARTALYSWLFAKNKGGEFVLRIEDTDLERNSQEAVDAILEG
CCCCCCCCCCCCEEEECCHHHHHHHHHHCCCCCCEEEEEEECCCCCCCHHHHHHHHHHH
MKWMGMEWDEGPYYQSKRFDRYNEVVDLLLSEDKAYKCYASKELLDEIRTEQEENKEMAR
HHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
YDANHPKIVAANAAAKEGDASVIRFRNPKEGSVVFDDQIRGRIEISNSQLDDLIIRRTDG
HCCCCCEEEEECCCCCCCCCCEEEECCCCCCCEEECCCCCCEEEECCCCCCCEEEEECCC
APTYNFVVVVDDWDMGITQVIRGEDHINNTPRQINIYEALGAPVPMFAHCAMILGDDGAK
CCCEEEEEEEECCCCCHHHHHCCCHHCCCCCCEEEEHHHHCCCCCHHHHHHHHCCCCCCH
LSKRHGAVSVMQYRDEGYLPNALNNYLVRLGWSHGDQEIFSQEEMINLFSLDAVSKSASA
HHHHCCCEEEEEECCCCCCCHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCC
FNTDKLRWLNNHYIKTSEPEYVANYLQWHLDQKEISLDNGPAITDVITLVSERCNTLIEL
CCCHHHHECCCCCCCCCCHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
ADQSRYFYEDFEAFDAGAAKKHLRGVAKGPLELALAKIEALQEWTTENLHNVIEEVCAEL
HHCCHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIGMGKIGMPLRVAVTGGGQSPSVDAVMQLIGKERVVTRIKMALAFIAEREANA
HCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA