Click here to switch to the map view.

The map label for this gene is flgJ [H]

Identifier: 209695757

GI number: 209695757

Start: 2505589

End: 2506587

Strand: Reverse

Name: flgJ [H]

Synonym: VSAL_I2330

Alternate gene names: 209695757

Gene position: 2506587-2505589 (Counterclockwise)

Preceding gene: 209695758

Following gene: 209695756

Centisome position: 75.38

GC content: 41.34

Gene sequence:

>999_bases
ATGAATAATTCAGTAAATATGACAGACGTTGGCTTTATCCACGATGTGGGTGGCCTAGATAAATTACGTAAAAGTGCGGT
AAATGGCGATCAAGAAAGCCAAGACAAAGCCTTGCGTGCGGCGGCTGTGCAGTTTGAATCGATCTTTACACAAATGCTTT
TTAAATCAATGCGTAATGCTAATAGTTCATTTAAATCAGACATGATGAGCAGTGATAATCAAAAGTTCTTTGAGCAAATG
CGTGACGACCAAATGTCGACCGAATTATCAAAGCAAGGCTCGCTAGGATTAGCGGATATGATTGTTGCACAACTTGGTGC
CTCACTAAATCAAGAACCGCCAGTGCACAATGGCAAGAATTTAGAGCAAAATGCACGAGTGGCAATGCGAAGAGCGGCAA
CGGATGAAACATTGCGTTTGCCTGCTGAGCATCAACCCCTGTCTGCGGCAAAACAAGCGTCACAAACCGAATCACCATCG
GCCGTTAAATTAGAACAAATGCTAGCGGCAAGACAAATTCAACAGTCTGAGAAGTTTGATTCACCTCAGCAATTTGTTGA
TACGATGAAGCCATACGCTGAAAAAGCGGCAAAGGCCTTAGGTATTGATTCAAATGTATTAATCGCTCAGGCCGCATTAG
AAACTGGCTGGGGTAAGAAAGTTATCAAGAATTCAGTGGATTCAAGCCATAACTTATTCAACATTAAAGCAGACAGAAGC
TGGCAAGGCGATAAGATCGCTAAAAATACGTTGGAATATCACGATAACATTGCCGTTTCAGAGAATGCCGCTTTCCGTTC
ATACGACAATTATGAAGAAAGCTTCAGTGACTTTGTGCGTTTCTTAAATCAAAATCCACGTTATGAACGCGCATTGCAAC
AAACACAAAGTTCAGAAAGCTTTATTAAAGGCATTCACAGTGCGGGTTATGCAACGGATCCTAATTACTCAAGTAAAGTA
ATGAGCGTAATGAAGACGGTTGAAGACTTACTAAAATAA

Upstream 100 bases:

>100_bases
TGTTTGTTATTTACAAAATTAGCAGCACGACGACTTGGCATTAAACTTGCTCTAGTTAATAGAGTGTTAGTTGGCCTGAT
TTTAAATAATAAGTGTGACG

Downstream 100 bases:

>100_bases
TCGCTCACAATGTAACCTCTTGAATCCTGTCTCTTAAATCTAAGTGGCAGGATTTTTTATTGCCGCTTATAAAAGGAAAG
AGGCAAAGAAAGGTGAGAGA

Product: flagellar rod assembly protein/muramidase FlgJ

Products: NA

Alternate protein names: Muramidase flgJ [H]

Number of amino acids: Translated: 332; Mature: 332

Protein sequence:

>332_residues
MNNSVNMTDVGFIHDVGGLDKLRKSAVNGDQESQDKALRAAAVQFESIFTQMLFKSMRNANSSFKSDMMSSDNQKFFEQM
RDDQMSTELSKQGSLGLADMIVAQLGASLNQEPPVHNGKNLEQNARVAMRRAATDETLRLPAEHQPLSAAKQASQTESPS
AVKLEQMLAARQIQQSEKFDSPQQFVDTMKPYAEKAAKALGIDSNVLIAQAALETGWGKKVIKNSVDSSHNLFNIKADRS
WQGDKIAKNTLEYHDNIAVSENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTQSSESFIKGIHSAGYATDPNYSSKV
MSVMKTVEDLLK

Sequences:

>Translated_332_residues
MNNSVNMTDVGFIHDVGGLDKLRKSAVNGDQESQDKALRAAAVQFESIFTQMLFKSMRNANSSFKSDMMSSDNQKFFEQM
RDDQMSTELSKQGSLGLADMIVAQLGASLNQEPPVHNGKNLEQNARVAMRRAATDETLRLPAEHQPLSAAKQASQTESPS
AVKLEQMLAARQIQQSEKFDSPQQFVDTMKPYAEKAAKALGIDSNVLIAQAALETGWGKKVIKNSVDSSHNLFNIKADRS
WQGDKIAKNTLEYHDNIAVSENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTQSSESFIKGIHSAGYATDPNYSSKV
MSVMKTVEDLLK
>Mature_332_residues
MNNSVNMTDVGFIHDVGGLDKLRKSAVNGDQESQDKALRAAAVQFESIFTQMLFKSMRNANSSFKSDMMSSDNQKFFEQM
RDDQMSTELSKQGSLGLADMIVAQLGASLNQEPPVHNGKNLEQNARVAMRRAATDETLRLPAEHQPLSAAKQASQTESPS
AVKLEQMLAARQIQQSEKFDSPQQFVDTMKPYAEKAAKALGIDSNVLIAQAALETGWGKKVIKNSVDSSHNLFNIKADRS
WQGDKIAKNTLEYHDNIAVSENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTQSSESFIKGIHSAGYATDPNYSSKV
MSVMKTVEDLLK

Specific function: Flagellum-specific muramidase which hydrolyzes the peptidoglycan layer to assemble the rod structure in the periplasmic space [H]

COG id: COG1705

COG function: function code NU; Muramidase (flagellum-specific)

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the glycosyl hydrolase 73 family [H]

Homologues:

Organism=Escherichia coli, GI1787321, Length=296, Percent_Identity=31.7567567567568, Blast_Score=137, Evalue=1e-33,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013377
- InterPro:   IPR000423
- InterPro:   IPR019301
- InterPro:   IPR013338
- InterPro:   IPR002901 [H]

Pfam domain/function: PF01832 Glucosaminidase; PF10135 Rod-binding [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36986; Mature: 36986

Theoretical pI: Translated: 6.27; Mature: 6.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNSVNMTDVGFIHDVGGLDKLRKSAVNGDQESQDKALRAAAVQFESIFTQMLFKSMRNA
CCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NSSFKSDMMSSDNQKFFEQMRDDQMSTELSKQGSLGLADMIVAQLGASLNQEPPVHNGKN
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
LEQNARVAMRRAATDETLRLPAEHQPLSAAKQASQTESPSAVKLEQMLAARQIQQSEKFD
CHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
SPQQFVDTMKPYAEKAAKALGIDSNVLIAQAALETGWGKKVIKNSVDSSHNLFNIKADRS
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCC
WQGDKIAKNTLEYHDNIAVSENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTQSSES
CCCHHHHHHHHHHHCCEEECCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIKGIHSAGYATDPNYSSKVMSVMKTVEDLLK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MNNSVNMTDVGFIHDVGGLDKLRKSAVNGDQESQDKALRAAAVQFESIFTQMLFKSMRNA
CCCCCCCCCCHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
NSSFKSDMMSSDNQKFFEQMRDDQMSTELSKQGSLGLADMIVAQLGASLNQEPPVHNGKN
CHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCC
LEQNARVAMRRAATDETLRLPAEHQPLSAAKQASQTESPSAVKLEQMLAARQIQQSEKFD
CHHHHHHHHHHHCCCHHHCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCC
SPQQFVDTMKPYAEKAAKALGIDSNVLIAQAALETGWGKKVIKNSVDSSHNLFNIKADRS
CHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCC
WQGDKIAKNTLEYHDNIAVSENAAFRSYDNYEESFSDFVRFLNQNPRYERALQQTQSSES
CCCHHHHHHHHHHHCCEEECCCCHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
FIKGIHSAGYATDPNYSSKVMSVMKTVEDLLK
HHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12620739 [H]