Click here to switch to the map view.

The map label for this gene is flaK [H]

Identifier: 209695743

GI number: 209695743

Start: 2484953

End: 2486434

Strand: Reverse

Name: flaK [H]

Synonym: VSAL_I2312

Alternate gene names: 209695743

Gene position: 2486434-2484953 (Counterclockwise)

Preceding gene: 209695744

Following gene: 209695742

Centisome position: 74.78

GC content: 40.82

Gene sequence:

>1482_bases
ATGCAAGGTTTAGCAAAGACACTAATTATTGAAGATGACGATCAGAACCGACATAATCTTGAGGTAATTCTTGAGTTTAT
TGGCGAGCATTGTCATGCTATCTCAACATCAGAACTGTCTACTTTTGACTGGAGTGATCCTTGGAGCGCATGCTTTTTAG
GTCAAGTTACTGATGAATCTTTAAAAAAATCAATCAAACACTCACTGGTTTCTCATAACCATATTCCCGTTATCATGATT
GCGGGGGCTACGCATGATATTGAGGATTTAACCAACTATGTGGGTGAATTGAATCAACCGATTAACTATCCTCAACTGAC
AGATGCCTTGCGTCATTGTCAAGAATTCATGGGTCGTCGTGGCTTAGGTATTCCTCATCTTGGTAGAAAAAATACGTTAT
TTCGTAGTATGGTTGGACAAAGTTCAGCGATTAGCGCCGTTCGTCATTTGATTGAGCAAGTGTCTGGTACCGAAGCAAGT
GTACTTGTATTGGGTGAATCTGGTACGGGTAAAGAAGTGGTTGCTCGTAATCTGCATTATCATTCTCCTCGTCGTAAAGG
ACCATTTGTACCGGTTAACTGTGGTGCGATTCCTCCTGATCTTCTTGAAAGTGAACTCTTTGGTCATGAAAAAGGCGCAT
TTACTGGGGCAATCACTGCTCGTAAAGGTCGTTTTGAATTGGCTCAAGGTGGTACGCTTTTCTTAGATGAAATCGGTGAT
ATGCCAATGTCGATGCAAGTGAAGTTACTTAGAGTACTTCAAGAGCGTTGTTTTGAGCGTGTTGGTGGTAATCAAAGTAT
TAAAGCGGATGTACGTATTGTTGCGGCAACGCATCGTAATCTTGACGACATGATTGAAAATAACAGTTTTCGTGAAGATC
TTTATTACCGCTTAAATGTCTTCCCAATTGAAATGCCTGCTTTGCGTGAACGTAAACAGGATATTCCGTTATTACTGCAA
GAATTACTGACAAGAATGGAAGCGGAGGGCAGTACTCGTGTGCGCTTAACGCCTCGTGCTGTTAATTCATTAATGGAACA
CCATTGGCCGGGTAACGTTCGTGAACTGGCGAATTTAATTGAACGCTTAATGATCTTATTTCCTGGTGAGTCGGTTGACG
TTAATCATTTACCCACTAAGTATCGTTACAGTGATATTCCTGAATTCCAGCCAGAAAACCACAATCAACAAAGTATTGAA
GAGCAAGAGCAAGCTGCGTTAGCGGATATGTTTGCAAACTTCAATGATGATGGTGTACCTCAAGTAAATAATCAAAGTTT
TGGGCCTTCTTCAAGTGATTTACCTGAAGATGGATTAAATCTAAAAGAGATGCTTGCGGATGTCGAAGTTAATATGATCT
GCCAAGCATTAGAAGCTCAGAACGGCATCGTTGCTCGTGCTGCTGATATGTTAGGTATGCGCCGTACAACTCTTGTTGAG
AAAATGCGTAAATACAATTTAAATAAAGATGACGTCAGATAG

Upstream 100 bases:

>100_bases
GTTGCCTTATTTTTCTTATGCTATAAAATGGTGGAACAATTTTGTTGCTGTTCAATGAATTGGCCAATTAAATCAAAGAA
TTAAGGCATATCACACTCCT

Downstream 100 bases:

>100_bases
TGTGTGACAATTAATTGACTCGTCACTGTCAATAAGTTTTCAATTTAACTTGTTGATAAGTAACAGTAAAAGGGCTGGCA
TGTAAAATGCATTAGCCCTT

Product: sigma 54 dependent transcription regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 493; Mature: 493

Protein sequence:

>493_residues
MQGLAKTLIIEDDDQNRHNLEVILEFIGEHCHAISTSELSTFDWSDPWSACFLGQVTDESLKKSIKHSLVSHNHIPVIMI
AGATHDIEDLTNYVGELNQPINYPQLTDALRHCQEFMGRRGLGIPHLGRKNTLFRSMVGQSSAISAVRHLIEQVSGTEAS
VLVLGESGTGKEVVARNLHYHSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
MPMSMQVKLLRVLQERCFERVGGNQSIKADVRIVAATHRNLDDMIENNSFREDLYYRLNVFPIEMPALRERKQDIPLLLQ
ELLTRMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLIERLMILFPGESVDVNHLPTKYRYSDIPEFQPENHNQQSIE
EQEQAALADMFANFNDDGVPQVNNQSFGPSSSDLPEDGLNLKEMLADVEVNMICQALEAQNGIVARAADMLGMRRTTLVE
KMRKYNLNKDDVR

Sequences:

>Translated_493_residues
MQGLAKTLIIEDDDQNRHNLEVILEFIGEHCHAISTSELSTFDWSDPWSACFLGQVTDESLKKSIKHSLVSHNHIPVIMI
AGATHDIEDLTNYVGELNQPINYPQLTDALRHCQEFMGRRGLGIPHLGRKNTLFRSMVGQSSAISAVRHLIEQVSGTEAS
VLVLGESGTGKEVVARNLHYHSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
MPMSMQVKLLRVLQERCFERVGGNQSIKADVRIVAATHRNLDDMIENNSFREDLYYRLNVFPIEMPALRERKQDIPLLLQ
ELLTRMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLIERLMILFPGESVDVNHLPTKYRYSDIPEFQPENHNQQSIE
EQEQAALADMFANFNDDGVPQVNNQSFGPSSSDLPEDGLNLKEMLADVEVNMICQALEAQNGIVARAADMLGMRRTTLVE
KMRKYNLNKDDVR
>Mature_493_residues
MQGLAKTLIIEDDDQNRHNLEVILEFIGEHCHAISTSELSTFDWSDPWSACFLGQVTDESLKKSIKHSLVSHNHIPVIMI
AGATHDIEDLTNYVGELNQPINYPQLTDALRHCQEFMGRRGLGIPHLGRKNTLFRSMVGQSSAISAVRHLIEQVSGTEAS
VLVLGESGTGKEVVARNLHYHSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
MPMSMQVKLLRVLQERCFERVGGNQSIKADVRIVAATHRNLDDMIENNSFREDLYYRLNVFPIEMPALRERKQDIPLLLQ
ELLTRMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLIERLMILFPGESVDVNHLPTKYRYSDIPEFQPENHNQQSIE
EQEQAALADMFANFNDDGVPQVNNQSFGPSSSDLPEDGLNLKEMLADVEVNMICQALEAQNGIVARAADMLGMRRTTLVE
KMRKYNLNKDDVR

Specific function: Required for the expression of the V-dependent nitrogen fixation system in Azotobacter vinelandii. It is required for the regulation of nitrogenase 2 transcription. Interacts with sigma- 54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI87082117, Length=361, Percent_Identity=42.6592797783933, Blast_Score=279, Evalue=3e-76,
Organism=Escherichia coli, GI1788550, Length=503, Percent_Identity=34.9900596421471, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1790299, Length=352, Percent_Identity=41.7613636363636, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1790437, Length=349, Percent_Identity=42.9799426934097, Blast_Score=261, Evalue=8e-71,
Organism=Escherichia coli, GI1789087, Length=360, Percent_Identity=40.5555555555556, Blast_Score=256, Evalue=2e-69,
Organism=Escherichia coli, GI1789233, Length=354, Percent_Identity=40.9604519774011, Blast_Score=246, Evalue=3e-66,
Organism=Escherichia coli, GI1788905, Length=221, Percent_Identity=53.8461538461538, Blast_Score=238, Evalue=4e-64,
Organism=Escherichia coli, GI87082152, Length=231, Percent_Identity=47.1861471861472, Blast_Score=216, Evalue=2e-57,
Organism=Escherichia coli, GI1786524, Length=256, Percent_Identity=45.3125, Blast_Score=212, Evalue=4e-56,
Organism=Escherichia coli, GI87081872, Length=229, Percent_Identity=44.5414847161572, Blast_Score=201, Evalue=1e-52,
Organism=Escherichia coli, GI1787583, Length=239, Percent_Identity=41.4225941422594, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI87081858, Length=369, Percent_Identity=28.9972899728997, Blast_Score=148, Evalue=7e-37,
Organism=Escherichia coli, GI1789828, Length=223, Percent_Identity=36.322869955157, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003018
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078 [H]

Pfam domain/function: PF01590 GAF; PF02954 HTH_8; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55450; Mature: 55450

Theoretical pI: Translated: 5.46; Mature: 5.46

Prosite motif: PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQGLAKTLIIEDDDQNRHNLEVILEFIGEHCHAISTSELSTFDWSDPWSACFLGQVTDES
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHH
LKKSIKHSLVSHNHIPVIMIAGATHDIEDLTNYVGELNQPINYPQLTDALRHCQEFMGRR
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
GLGIPHLGRKNTLFRSMVGQSSAISAVRHLIEQVSGTEASVLVLGESGTGKEVVARNLHY
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
HSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCEEEEHHHCC
MPMSMQVKLLRVLQERCFERVGGNQSIKADVRIVAATHRNLDDMIENNSFREDLYYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEEHHCCHHHHHHCCCHHHHHEEEEEE
FPIEMPALRERKQDIPLLLQELLTRMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLI
EEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHH
ERLMILFPGESVDVNHLPTKYRYSDIPEFQPENHNQQSIEEQEQAALADMFANFNDDGVP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
QVNNQSFGPSSSDLPEDGLNLKEMLADVEVNMICQALEAQNGIVARAADMLGMRRTTLVE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMRKYNLNKDDVR
HHHHCCCCCCCCC
>Mature Secondary Structure
MQGLAKTLIIEDDDQNRHNLEVILEFIGEHCHAISTSELSTFDWSDPWSACFLGQVTDES
CCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHCCCCHHH
LKKSIKHSLVSHNHIPVIMIAGATHDIEDLTNYVGELNQPINYPQLTDALRHCQEFMGRR
HHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCC
GLGIPHLGRKNTLFRSMVGQSSAISAVRHLIEQVSGTEASVLVLGESGTGKEVVARNLHY
CCCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHC
HSPRRKGPFVPVNCGAIPPDLLESELFGHEKGAFTGAITARKGRFELAQGGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCEEEEEEECCCCEEECCCCEEEEHHHCC
MPMSMQVKLLRVLQERCFERVGGNQSIKADVRIVAATHRNLDDMIENNSFREDLYYRLNV
CCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEEHHCCHHHHHHCCCHHHHHEEEEEE
FPIEMPALRERKQDIPLLLQELLTRMEAEGSTRVRLTPRAVNSLMEHHWPGNVRELANLI
EEECCHHHHHHHCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHCCCCCHHHHHHHH
ERLMILFPGESVDVNHLPTKYRYSDIPEFQPENHNQQSIEEQEQAALADMFANFNDDGVP
HHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCC
QVNNQSFGPSSSDLPEDGLNLKEMLADVEVNMICQALEAQNGIVARAADMLGMRRTTLVE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
KMRKYNLNKDDVR
HHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722750 [H]