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The map label for this gene is flaM [H]

Identifier: 209695741

GI number: 209695741

Start: 2482344

End: 2483780

Strand: Reverse

Name: flaM [H]

Synonym: VSAL_I2310

Alternate gene names: 209695741

Gene position: 2483780-2482344 (Counterclockwise)

Preceding gene: 209695742

Following gene: 209695740

Centisome position: 74.7

GC content: 42.03

Gene sequence:

>1437_bases
ATGGCTCAAAGCAAAGTATTAATTGTTGAAGATGATGAAGGTCTACGCGAAGCATTGATCGACACATTGGAATTGGCAGG
GTATGAGTGGGTAGAGGCGGATAGTGCGGAAACTGCATTACTTATCTTGAAGAAAGAACCCGTTGATATTGTTGTTTCTG
ATGTGCAAATGGCAGGAATGGGTGGATTAGCCTTGCTTCGTAGCATTAAATTAAATTGGCCGAATTTGCCCGTTTTATTG
ATGACCGCTTACGCCAATATTGAAGATGCCGTTTCAGCAATGAAAGATGGCGCAATTGATTACATGGCGAAGCCGTTTGC
GCCAGAAGTGCTGCTTAATATGGTTAGTCGCTATGCGCCAGTTAAAAACGAACAAACAGGCAATGCCATTGTTGCTGATG
AAAAAAGCTTAAAATTACTTAAGTTAGCAGACAAAGTGGCAAGAACTGACGCGAACGTGATGGTGCTTGGACCTAGTGGT
TCAGGTAAAGAAGTGATGTCTCGCTATATTCATAATAATTCCCAACGCAGTGAAGGGCCTTTTATTGCCATTAACTGTGC
GGCAATTCCAGATAATATGTTGGAAGCAACCTTATTTGGTTATGAGAAAGGTGCCTTTACTGGTGCAGTTCAAGCGTGTC
CGGGCAAGTTTGAGCAAGCTCAAGGTGGCACTATCTTACTGGATGAAATCAGTGAAATGGACCTTAACTTACAAGTTAAA
TTGCTTCGAGTGCTTCAAGAGCGAGAAGTGGAACGTTTAGGTGGCCGTAAGAGCATCAAATTAGATGTTCGTATTTTGGC
AACCAGTAACCGAGATCTTAAGAAATACGTTGAAGAAGGTAATTTCCGTGAGGATTTATATTATCGACTAAACGTGTTCC
CAATTGCATGGCCGCCATTAAAAGAACGTAAAGGGGATATTATTCCTCTGGCTCAACATTTGGTTGAGCGTCACTGTCAA
AAACAAGGTCTGCCAGTGCCGACGTTAACCTCTGAAGCGATTGCCAAATTAGAGATTTATCCTTGGCCTGGTAACGTACG
TGAATTAGACAATGTAGTTCAACGTGCCCTTATTTTGGCAGAGGAGAATTACATCACTGCGGATGACATATTACTTGAAG
GGGTTGATTGGGCTGATGCAAGTAGTTTACAGAAAATAGTCAGTGGTGAAGTGCCAACGATTAAGCCTGAGCAACAAATA
CCGTCAATAAATGAACCTTCAGTTGAATCAACAGATAGCGTCAATATGGATGCATCAAGTTCGTCGACAGGGCTGGGTGG
GGAATTACGTGAGCAAGAATACATTATTATTCTTGAAACGATTAAAGCGTGTGATGGAAAGCGCAAAGAAGTGGCTGAAA
AGCTGGGTATTAGCCCAAGAACACTGAGATATAAATTAGCAAAAATGCGTGATGCTGGAATAGATATCCCAAGTTAA

Upstream 100 bases:

>100_bases
ACAAAGGGCAATTAGATTTGTATTCAAAACCAGATGATGGTGCGTGCTTCACCATGTCAATTCCTCTTACCACTACTCAA
ACTGATTCTGGAGAATAACA

Downstream 100 bases:

>100_bases
ACAACGGGTTAAGTTTATTGATATTTTGGTATGTAAATTGCTTTATATACTTCGGGTCGTAACATTAAGTCAATTAGTTG
ACTGGGTCGGAGAAAGATAA

Product: sigma-54 dependent response regulator

Products: NA

Alternate protein names: Ornithine decarboxylase antizyme; Ornithine/arginine decarboxylase inhibitor [H]

Number of amino acids: Translated: 478; Mature: 477

Protein sequence:

>478_residues
MAQSKVLIVEDDEGLREALIDTLELAGYEWVEADSAETALLILKKEPVDIVVSDVQMAGMGGLALLRSIKLNWPNLPVLL
MTAYANIEDAVSAMKDGAIDYMAKPFAPEVLLNMVSRYAPVKNEQTGNAIVADEKSLKLLKLADKVARTDANVMVLGPSG
SGKEVMSRYIHNNSQRSEGPFIAINCAAIPDNMLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEISEMDLNLQVK
LLRVLQEREVERLGGRKSIKLDVRILATSNRDLKKYVEEGNFREDLYYRLNVFPIAWPPLKERKGDIIPLAQHLVERHCQ
KQGLPVPTLTSEAIAKLEIYPWPGNVRELDNVVQRALILAEENYITADDILLEGVDWADASSLQKIVSGEVPTIKPEQQI
PSINEPSVESTDSVNMDASSSSTGLGGELREQEYIIILETIKACDGKRKEVAEKLGISPRTLRYKLAKMRDAGIDIPS

Sequences:

>Translated_478_residues
MAQSKVLIVEDDEGLREALIDTLELAGYEWVEADSAETALLILKKEPVDIVVSDVQMAGMGGLALLRSIKLNWPNLPVLL
MTAYANIEDAVSAMKDGAIDYMAKPFAPEVLLNMVSRYAPVKNEQTGNAIVADEKSLKLLKLADKVARTDANVMVLGPSG
SGKEVMSRYIHNNSQRSEGPFIAINCAAIPDNMLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEISEMDLNLQVK
LLRVLQEREVERLGGRKSIKLDVRILATSNRDLKKYVEEGNFREDLYYRLNVFPIAWPPLKERKGDIIPLAQHLVERHCQ
KQGLPVPTLTSEAIAKLEIYPWPGNVRELDNVVQRALILAEENYITADDILLEGVDWADASSLQKIVSGEVPTIKPEQQI
PSINEPSVESTDSVNMDASSSSTGLGGELREQEYIIILETIKACDGKRKEVAEKLGISPRTLRYKLAKMRDAGIDIPS
>Mature_477_residues
AQSKVLIVEDDEGLREALIDTLELAGYEWVEADSAETALLILKKEPVDIVVSDVQMAGMGGLALLRSIKLNWPNLPVLLM
TAYANIEDAVSAMKDGAIDYMAKPFAPEVLLNMVSRYAPVKNEQTGNAIVADEKSLKLLKLADKVARTDANVMVLGPSGS
GKEVMSRYIHNNSQRSEGPFIAINCAAIPDNMLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEISEMDLNLQVKL
LRVLQEREVERLGGRKSIKLDVRILATSNRDLKKYVEEGNFREDLYYRLNVFPIAWPPLKERKGDIIPLAQHLVERHCQK
QGLPVPTLTSEAIAKLEIYPWPGNVRELDNVVQRALILAEENYITADDILLEGVDWADASSLQKIVSGEVPTIKPEQQIP
SINEPSVESTDSVNMDASSSSTGLGGELREQEYIIILETIKACDGKRKEVAEKLGISPRTLRYKLAKMRDAGIDIPS

Specific function: Member of the two-component regulatory system AtoS/AtoC involved in the transcriptional regulation of the ato genes for acetoacetate metabolism. Also an inhibitor of polyamine biosynthesis [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=489, Percent_Identity=38.4458077709611, Blast_Score=329, Evalue=3e-91,
Organism=Escherichia coli, GI1790437, Length=473, Percent_Identity=37.4207188160677, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1788905, Length=375, Percent_Identity=42.4, Blast_Score=288, Evalue=5e-79,
Organism=Escherichia coli, GI1790299, Length=480, Percent_Identity=33.75, Blast_Score=285, Evalue=5e-78,
Organism=Escherichia coli, GI87082117, Length=245, Percent_Identity=47.7551020408163, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1789087, Length=358, Percent_Identity=38.8268156424581, Blast_Score=231, Evalue=6e-62,
Organism=Escherichia coli, GI87082152, Length=241, Percent_Identity=45.2282157676349, Blast_Score=224, Evalue=1e-59,
Organism=Escherichia coli, GI1789233, Length=337, Percent_Identity=39.1691394658754, Blast_Score=216, Evalue=3e-57,
Organism=Escherichia coli, GI87081872, Length=238, Percent_Identity=41.5966386554622, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1787583, Length=305, Percent_Identity=35.7377049180328, Blast_Score=191, Evalue=1e-49,
Organism=Escherichia coli, GI1786524, Length=230, Percent_Identity=41.7391304347826, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1789828, Length=240, Percent_Identity=35, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI87081858, Length=343, Percent_Identity=29.7376093294461, Blast_Score=140, Evalue=2e-34,
Organism=Escherichia coli, GI1786599, Length=152, Percent_Identity=27.6315789473684, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1788394, Length=129, Percent_Identity=28.6821705426357, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787375, Length=105, Percent_Identity=35.2380952380952, Blast_Score=63, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 52671; Mature: 52540

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQSKVLIVEDDEGLREALIDTLELAGYEWVEADSAETALLILKKEPVDIVVSDVQMAGM
CCCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCEEEEECHHHCCC
GGLALLRSIKLNWPNLPVLLMTAYANIEDAVSAMKDGAIDYMAKPFAPEVLLNMVSRYAP
CHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCC
VKNEQTGNAIVADEKSLKLLKLADKVARTDANVMVLGPSGSGKEVMSRYIHNNSQRSEGP
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCC
FIAINCAAIPDNMLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEISEMDLNLQVK
EEEEEEEECCCHHHHHHHHCCCCCCCCCHHHHCCCCCHHCCCCEEEEECHHCCCCCHHHH
LLRVLQEREVERLGGRKSIKLDVRILATSNRDLKKYVEEGNFREDLYYRLNVFPIAWPPL
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHCCCCCCCEEEEEEEEEECCCCH
KERKGDIIPLAQHLVERHCQKQGLPVPTLTSEAIAKLEIYPWPGNVRELDNVVQRALILA
HHCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHC
EENYITADDILLEGVDWADASSLQKIVSGEVPTIKPEQQIPSINEPSVESTDSVNMDASS
CCCCEEHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
SSTGLGGELREQEYIIILETIKACDGKRKEVAEKLGISPRTLRYKLAKMRDAGIDIPS
CCCCCCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AQSKVLIVEDDEGLREALIDTLELAGYEWVEADSAETALLILKKEPVDIVVSDVQMAGM
CCCCEEEEECCCHHHHHHHHHHHHCCCCEEECCCCCEEEEEEECCCCEEEEECHHHCCC
GGLALLRSIKLNWPNLPVLLMTAYANIEDAVSAMKDGAIDYMAKPFAPEVLLNMVSRYAP
CHHHHHHHHHCCCCCCCEEEEEHHCCHHHHHHHHHCCCHHHHCCCCCHHHHHHHHHHHCC
VKNEQTGNAIVADEKSLKLLKLADKVARTDANVMVLGPSGSGKEVMSRYIHNNSQRSEGP
CCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCEEEECCCCCCHHHHHHHHHCCCCCCCCC
FIAINCAAIPDNMLEATLFGYEKGAFTGAVQACPGKFEQAQGGTILLDEISEMDLNLQVK
EEEEEEEECCCHHHHHHHHCCCCCCCCCHHHHCCCCCHHCCCCEEEEECHHCCCCCHHHH
LLRVLQEREVERLGGRKSIKLDVRILATSNRDLKKYVEEGNFREDLYYRLNVFPIAWPPL
HHHHHHHHHHHHHCCCCEEEEEEEEEEECCHHHHHHHHCCCCCCCEEEEEEEEEECCCCH
KERKGDIIPLAQHLVERHCQKQGLPVPTLTSEAIAKLEIYPWPGNVRELDNVVQRALILA
HHCCCCEEHHHHHHHHHHHHHCCCCCCCCCHHHHEEEEEEECCCCHHHHHHHHHHHHHHC
EENYITADDILLEGVDWADASSLQKIVSGEVPTIKPEQQIPSINEPSVESTDSVNMDASS
CCCCEEHHHHHCCCCCCCCHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCC
SSTGLGGELREQEYIIILETIKACDGKRKEVAEKLGISPRTLRYKLAKMRDAGIDIPS
CCCCCCCCCCCCCEEEEEEEHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097040; 9278503; 8346225 [H]