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The map label for this gene is fliA [H]

Identifier: 209695722

GI number: 209695722

Start: 2463223

End: 2463954

Strand: Reverse

Name: fliA [H]

Synonym: VSAL_I2290

Alternate gene names: 209695722

Gene position: 2463954-2463223 (Counterclockwise)

Preceding gene: 209695723

Following gene: 209695721

Centisome position: 74.1

GC content: 38.8

Gene sequence:

>732_bases
ATGTCTAATACCTTAACTTACGATCATAAAGGCAATGTGAACAGTCAGAAAGCATTTTTAGAGCGCTATTCTGAACTGGT
AAAACGCATTGCCCATCACTTAATGGCAAGATTACCAGCAAGTGTATTAGTTGATGATTTAATACAGTCTGGAATGATCG
GCCTGTTAGAAGCACAACAAAATTATGACGGCAGTAAAGGCGCAAGTTTTGAAACATACGCTGGAATAAGAATCAGAGGT
TCGATGCTGGATGAAATTCGACGCGGGGACTGGGTTCCTCGCTCTGTTCATAAGAATAGTCGAATGGTTAATGCAGCAAT
TAAAGCACTTGAAGCTAAACTTTCTCGCGAACCGACCGATAAAGAGATAGCAGAACATTTAGAATTACCGTTAGATCAGT
ATTATCAGATACTTAATGATGTAAATTGCGGTAAACTTGTAGGTATGGAAGATTTAGGCGTTTCCGAAGATGTAATAACA
ACGGAAGAACACGCTGATGATAATATTCCATTTCAAGGTGTTGTAGATGAACATTTTCGAGTTGCTCTTGTGGAGGCGAT
TAAAACCCTTCCAGAAAGAGAAGCTTTAGTTTTATCTCTTTATTATGATGAAGAGTTAAATTTGAAAGAAATTGGTGCAG
TTATTGGTGTCAGCGAGTCTCGTGTCAGCCAAATACACAGTCAATCAATGCAACGACTACGGGCCAAGTTATCAACTTGG
ACAGTAGAATAA

Upstream 100 bases:

>100_bases
ATCGTGACATGGCCTGTGCCTAAAGCACCAAGTGGACATTTAGAATTTTTTGTAGAGAAACTGCTACGAAATGAAAAAAC
CAGCGAAGTAGAGGACGACT

Downstream 100 bases:

>100_bases
TTAAATCCCGTTACTTTGTGCCCTCACTGGAGGCGATTTTGAATAAAAACATGAAGATCCTTATTGTTGATGACTTCTCA
ACAATGCGCCGTATTGTAAA

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 243; Mature: 242

Protein sequence:

>243_residues
MSNTLTYDHKGNVNSQKAFLERYSELVKRIAHHLMARLPASVLVDDLIQSGMIGLLEAQQNYDGSKGASFETYAGIRIRG
SMLDEIRRGDWVPRSVHKNSRMVNAAIKALEAKLSREPTDKEIAEHLELPLDQYYQILNDVNCGKLVGMEDLGVSEDVIT
TEEHADDNIPFQGVVDEHFRVALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSTW
TVE

Sequences:

>Translated_243_residues
MSNTLTYDHKGNVNSQKAFLERYSELVKRIAHHLMARLPASVLVDDLIQSGMIGLLEAQQNYDGSKGASFETYAGIRIRG
SMLDEIRRGDWVPRSVHKNSRMVNAAIKALEAKLSREPTDKEIAEHLELPLDQYYQILNDVNCGKLVGMEDLGVSEDVIT
TEEHADDNIPFQGVVDEHFRVALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSTW
TVE
>Mature_242_residues
SNTLTYDHKGNVNSQKAFLERYSELVKRIAHHLMARLPASVLVDDLIQSGMIGLLEAQQNYDGSKGASFETYAGIRIRGS
MLDEIRRGDWVPRSVHKNSRMVNAAIKALEAKLSREPTDKEIAEHLELPLDQYYQILNDVNCGKLVGMEDLGVSEDVITT
EEHADDNIPFQGVVDEHFRVALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSTWT
VE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=229, Percent_Identity=51.0917030567686, Blast_Score=212, Evalue=2e-56,
Organism=Escherichia coli, GI1789448, Length=204, Percent_Identity=28.4313725490196, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789098, Length=241, Percent_Identity=28.6307053941909, Blast_Score=70, Evalue=9e-14,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27363; Mature: 27232

Theoretical pI: Translated: 5.06; Mature: 5.06

Prosite motif: PS00018 EF_HAND_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTLTYDHKGNVNSQKAFLERYSELVKRIAHHLMARLPASVLVDDLIQSGMIGLLEAQQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
NYDGSKGASFETYAGIRIRGSMLDEIRRGDWVPRSVHKNSRMVNAAIKALEAKLSREPTD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
KEIAEHLELPLDQYYQILNDVNCGKLVGMEDLGVSEDVITTEEHADDNIPFQGVVDEHFR
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCHHCCCCCCCCCCHHHHHHH
VALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSTW
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TVE
CCC
>Mature Secondary Structure 
SNTLTYDHKGNVNSQKAFLERYSELVKRIAHHLMARLPASVLVDDLIQSGMIGLLEAQQ
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHH
NYDGSKGASFETYAGIRIRGSMLDEIRRGDWVPRSVHKNSRMVNAAIKALEAKLSREPTD
CCCCCCCCCCCCCCCEEEEHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCH
KEIAEHLELPLDQYYQILNDVNCGKLVGMEDLGVSEDVITTEEHADDNIPFQGVVDEHFR
HHHHHHHCCCHHHHHHHHCCCCCCCCCCCHHCCCCCCCCCCHHCCCCCCCCCCHHHHHHH
VALVEAIKTLPEREALVLSLYYDEELNLKEIGAVIGVSESRVSQIHSQSMQRLRAKLSTW
HHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
TVE
CCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]