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The map label for this gene is vacJ [H]

Identifier: 209695704

GI number: 209695704

Start: 2447622

End: 2448407

Strand: Reverse

Name: vacJ [H]

Synonym: VSAL_I2272

Alternate gene names: 209695704

Gene position: 2448407-2447622 (Counterclockwise)

Preceding gene: 209695705

Following gene: 209695700

Centisome position: 73.63

GC content: 38.93

Gene sequence:

>786_bases
GTGTATAAAAAAATATGTTTTTCTTGGGTGCTACTTTTTGTTATCACGGGGTGTTCTCAAACACCTGAGTCGATTGATCC
TGAAATAGAAACCAATGAGGCTGTCGAAGTTGAATACGATGACTCGCATGATGGTGACCCAATTGAAGGGTTTAACCGAG
TTATGTGGGATCTTAACTATAATATTTTAGACCCTTACTTAGTTCGTCCGGTATCTATTGCCTATGTTGATTATACGCCA
AGCCCAATTCGAATCGGGATCGCAAATTTCTTAGCGAACTTAGATGAACCCTCAAGCATGGTAAATAATTTGATTATGGG
GAATGGTGAGCAAGCAATGGTGAATTTCAATCGATTTTGGTTGAACACAACACTAGGTCTTGTTGGACTTATTGATATTG
CCAGTGCTGCTGAGATTCAAAAACCAGACAGTCGCTCTTTTGGGGATGCATTAGGTTATTATGGTGTCGGTAACGGGCCT
TACTTTATGTTACCTGGTTATGGTCCATGGACATTGCGTGAAGCTGGTGATTTTGTTGATGGCTTATACATGCCGTTATC
GTATTTAAACTTTTGGCAAGGTTTAGGTAAATGGGGATTAGAAGGCATGGAGTCACGAGTTGGTTTAATATCTCAAGAGC
CAATGCTAGAAACGTCACCCGATCCCTACGTATTAACCCGTGATGTTTATTTACAAAGACAAGATTTTAAAGCAGAAATA
GAACCAGAAAGTGATAGTAATTCAGAAGAAGACTTAAATGACTTCTTAGATGAAATTGATGAATAA

Upstream 100 bases:

>100_bases
GTTTTTTTAATGACTATAATTGTATAACTATAATAATAAATAAAGAGTACAGCCGAGCAAGTTACTCGTTCAGTTAGTCT
CAGTAGGTTAAAGGTGTGTT

Downstream 100 bases:

>100_bases
TTGATCTGTTCAGAGTTCAGAGCAAATACTGCCTGAACTCTAGTTATTCTTTTTACGTTGAACCATCATCAATAATAAAG
CGACGAGTGATCCTGAAGTG

Product: lipoprotein VacJ precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 261; Mature: 261

Protein sequence:

>261_residues
MYKKICFSWVLLFVITGCSQTPESIDPEIETNEAVEVEYDDSHDGDPIEGFNRVMWDLNYNILDPYLVRPVSIAYVDYTP
SPIRIGIANFLANLDEPSSMVNNLIMGNGEQAMVNFNRFWLNTTLGLVGLIDIASAAEIQKPDSRSFGDALGYYGVGNGP
YFMLPGYGPWTLREAGDFVDGLYMPLSYLNFWQGLGKWGLEGMESRVGLISQEPMLETSPDPYVLTRDVYLQRQDFKAEI
EPESDSNSEEDLNDFLDEIDE

Sequences:

>Translated_261_residues
MYKKICFSWVLLFVITGCSQTPESIDPEIETNEAVEVEYDDSHDGDPIEGFNRVMWDLNYNILDPYLVRPVSIAYVDYTP
SPIRIGIANFLANLDEPSSMVNNLIMGNGEQAMVNFNRFWLNTTLGLVGLIDIASAAEIQKPDSRSFGDALGYYGVGNGP
YFMLPGYGPWTLREAGDFVDGLYMPLSYLNFWQGLGKWGLEGMESRVGLISQEPMLETSPDPYVLTRDVYLQRQDFKAEI
EPESDSNSEEDLNDFLDEIDE
>Mature_261_residues
MYKKICFSWVLLFVITGCSQTPESIDPEIETNEAVEVEYDDSHDGDPIEGFNRVMWDLNYNILDPYLVRPVSIAYVDYTP
SPIRIGIANFLANLDEPSSMVNNLIMGNGEQAMVNFNRFWLNTTLGLVGLIDIASAAEIQKPDSRSFGDALGYYGVGNGP
YFMLPGYGPWTLREAGDFVDGLYMPLSYLNFWQGLGKWGLEGMESRVGLISQEPMLETSPDPYVLTRDVYLQRQDFKAEI
EPESDSNSEEDLNDFLDEIDE

Specific function: Actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner membrane [H]

COG id: COG2853

COG function: function code M; Surface lipoprotein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaA family [H]

Homologues:

Organism=Escherichia coli, GI1788688, Length=228, Percent_Identity=46.9298245614035, Blast_Score=216, Evalue=9e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007428 [H]

Pfam domain/function: PF04333 VacJ [H]

EC number: NA

Molecular weight: Translated: 29469; Mature: 29469

Theoretical pI: Translated: 3.73; Mature: 3.73

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKKICFSWVLLFVITGCSQTPESIDPEIETNEAVEVEYDDSHDGDPIEGFNRVMWDLNY
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHEEEECCC
NILDPYLVRPVSIAYVDYTPSPIRIGIANFLANLDEPSSMVNNLIMGNGEQAMVNFNRFW
CCCCHHHHCEEEEEEEECCCCCCEEEHHHHHCCCCCHHHHHHHHHCCCCCEEEEEHHHHH
LNTTLGLVGLIDIASAAEIQKPDSRSFGDALGYYGVGNGPYFMLPGYGPWTLREAGDFVD
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHH
GLYMPLSYLNFWQGLGKWGLEGMESRVGLISQEPMLETSPDPYVLTRDVYLQRQDFKAEI
HHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHEEECCCCCEEE
EPESDSNSEEDLNDFLDEIDE
CCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure
MYKKICFSWVLLFVITGCSQTPESIDPEIETNEAVEVEYDDSHDGDPIEGFNRVMWDLNY
CCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHEEEECCC
NILDPYLVRPVSIAYVDYTPSPIRIGIANFLANLDEPSSMVNNLIMGNGEQAMVNFNRFW
CCCCHHHHCEEEEEEEECCCCCCEEEHHHHHCCCCCHHHHHHHHHCCCCCEEEEEHHHHH
LNTTLGLVGLIDIASAAEIQKPDSRSFGDALGYYGVGNGPYFMLPGYGPWTLREAGDFVD
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCCCCCCCCEEEECCCCCCCHHHHHHHHH
GLYMPLSYLNFWQGLGKWGLEGMESRVGLISQEPMLETSPDPYVLTRDVYLQRQDFKAEI
HHHHHHHHHHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCEEEEHHHEEECCCCCEEE
EPESDSNSEEDLNDFLDEIDE
CCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9205837; 9278503 [H]