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The map label for this gene is fadI [H]

Identifier: 209695700

GI number: 209695700

Start: 2441416

End: 2442759

Strand: Reverse

Name: fadI [H]

Synonym: VSAL_I2268

Alternate gene names: 209695700

Gene position: 2442759-2441416 (Counterclockwise)

Preceding gene: 209695704

Following gene: 209695699

Centisome position: 73.46

GC content: 43.15

Gene sequence:

>1344_bases
ATGTCAAACTCACAACGTTCAAGCTCATCATTATCAAGACATTATCAGCCATTAACGACACGACAAGGTGACCGTATTGC
TGTGGTTTCTGGGATCCGAACTCCTTTTGCTAAGCAATCTACCGCCTTTAGTTCAACGCCTGCGGTTGATCTGGGTAAAT
TGGCAGTAAAAGCTTTGATGGATAAAACTGAGATCGATCCTAAATTGATTGATCAAGTTGTGTTTGGTCAGGTTGTTCAA
ATGCCAGAAGCCCCGAATATTGCTCGTGAAATTGTTCTTGGTACAGGAATGAATGTGGGGACGGATGCGTATAGTGTTAC
GCGTGCCTGTGCGACCAGCTTTCAAACAACAGCCAATGTTGTTGAAAGTATCATGGCAGGATCGATTGATATCGGAATTG
CAGGCGGGGCTGATTCTTCGTCAGTACTGCCAATCGGCGTGTCAAAAAAATTGGCTTCAACCTTATTAGCATTAAGTAAA
ACCAAAACGGTAGGACAAAAACTGTCTTTATTACGAACGTTATCGTTAAAAGACATTGCTCCTGTACCTCCTGCCGTTGC
TGAATATTCAACGGGGATCTCGATGGGGCAAACCGCAGAACAAATGGCAAAAAGTCACGGTATTACCCGAGAAGAACAAG
ATGCATTGGCTCACCGCTCTCATTCTTTGGCAGCAAAAGCATGGCAAGATGGGTTAATTCAAGACGAAGTGATGACTGCA
TTCCCTGATCCCTATAAAGAGTGGATCGATCAAGACAACAATATTCGCTATAACTCTGATCTGGCCAGTTACGCAAAATT
ACGTCCCGCGTTTGATAGAAAATACGGTTCAGTAACGGCAGCAAACAGTACACCATTAACCGATGGTGGAGCCGCAATTT
TATTGATGAGTGAAAAGCGAGCAAAAGAATTAGGTTATGAACCGCTTGGCTATATTCGTTCATTTGCGTTTAGTGCAATG
GATGTACATCACGATATGTTAATGGGTCCTTCTTACGCAACCCCAATCGCATTAGATAAAGCAGGAATTTCATTATCTGA
TCTTACTTTAATTGATATGCATGAAGCCTTTGCAGCGCAAACATTATCGAACGTGAAAATGTTTTCCTCAACCAAGTTTG
CTAAAGAGTGTCTTGGCAGAGATAAAGCCATTGGGGAGATCGATATGGATAAATTCAATGTCCTTGGCGGTTCGTTGGCT
TATGGCCACCCTTTTGCTGCAACTGGAGCGAGAATGATCATTCAAACGTTGCGAGAATTAAAGCGTAGAGGTGGTGGTTT
TGGATTAAATACAGCGTGTGCTGCTGGTGGTCTAGGTGCTGCAATGGTGTTGGAGGTTGAATAA

Upstream 100 bases:

>100_bases
CCCTATATCACATTTCTACTATAATCTACTTTTCAAAATTAACAAGTTAGTTACAATTTGAGGTCTGACCACTAATTTGA
TATTTTCGCAAGGAGACGCG

Downstream 100 bases:

>100_bases
ATGACAGTAGAAAACATAAATCAAAAGGCAGAAAGTAATAAAGAAACCAACAGTGCCTTTGTGTGGCATAAAGATGCCGA
TAATTTGGCTTGGCTGACGA

Product: 3-ketoacyl-CoA thiolase

Products: NA

Alternate protein names: ACSs; Acetyl-CoA acyltransferase; Acyl-CoA ligase; Beta-ketothiolase; Fatty acid oxidation complex subunit beta [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MSNSQRSSSSLSRHYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSSTPAVDLGKLAVKALMDKTEIDPKLIDQVVFGQVVQ
MPEAPNIAREIVLGTGMNVGTDAYSVTRACATSFQTTANVVESIMAGSIDIGIAGGADSSSVLPIGVSKKLASTLLALSK
TKTVGQKLSLLRTLSLKDIAPVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHSLAAKAWQDGLIQDEVMTA
FPDPYKEWIDQDNNIRYNSDLASYAKLRPAFDRKYGSVTAANSTPLTDGGAAILLMSEKRAKELGYEPLGYIRSFAFSAM
DVHHDMLMGPSYATPIALDKAGISLSDLTLIDMHEAFAAQTLSNVKMFSSTKFAKECLGRDKAIGEIDMDKFNVLGGSLA
YGHPFAATGARMIIQTLRELKRRGGGFGLNTACAAGGLGAAMVLEVE

Sequences:

>Translated_447_residues
MSNSQRSSSSLSRHYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSSTPAVDLGKLAVKALMDKTEIDPKLIDQVVFGQVVQ
MPEAPNIAREIVLGTGMNVGTDAYSVTRACATSFQTTANVVESIMAGSIDIGIAGGADSSSVLPIGVSKKLASTLLALSK
TKTVGQKLSLLRTLSLKDIAPVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHSLAAKAWQDGLIQDEVMTA
FPDPYKEWIDQDNNIRYNSDLASYAKLRPAFDRKYGSVTAANSTPLTDGGAAILLMSEKRAKELGYEPLGYIRSFAFSAM
DVHHDMLMGPSYATPIALDKAGISLSDLTLIDMHEAFAAQTLSNVKMFSSTKFAKECLGRDKAIGEIDMDKFNVLGGSLA
YGHPFAATGARMIIQTLRELKRRGGGFGLNTACAAGGLGAAMVLEVE
>Mature_446_residues
SNSQRSSSSLSRHYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSSTPAVDLGKLAVKALMDKTEIDPKLIDQVVFGQVVQM
PEAPNIAREIVLGTGMNVGTDAYSVTRACATSFQTTANVVESIMAGSIDIGIAGGADSSSVLPIGVSKKLASTLLALSKT
KTVGQKLSLLRTLSLKDIAPVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHSLAAKAWQDGLIQDEVMTAF
PDPYKEWIDQDNNIRYNSDLASYAKLRPAFDRKYGSVTAANSTPLTDGGAAILLMSEKRAKELGYEPLGYIRSFAFSAMD
VHHDMLMGPSYATPIALDKAGISLSDLTLIDMHEAFAAQTLSNVKMFSSTKFAKECLGRDKAIGEIDMDKFNVLGGSLAY
GHPFAATGARMIIQTLRELKRRGGGFGLNTACAAGGLGAAMVLEVE

Specific function: Catalyzes the final step of fatty acid oxidation in which acetyl-CoA is released and the CoA ester of a fatty acid two carbons shorter is formed [H]

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4504327, Length=420, Percent_Identity=42.1428571428571, Blast_Score=349, Evalue=3e-96,
Organism=Homo sapiens, GI4501853, Length=430, Percent_Identity=31.6279069767442, Blast_Score=191, Evalue=1e-48,
Organism=Homo sapiens, GI4557237, Length=423, Percent_Identity=29.7872340425532, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI167614485, Length=423, Percent_Identity=30.0236406619385, Blast_Score=182, Evalue=4e-46,
Organism=Homo sapiens, GI148539872, Length=428, Percent_Identity=28.7383177570093, Blast_Score=167, Evalue=3e-41,
Organism=Homo sapiens, GI194353979, Length=106, Percent_Identity=36.7924528301887, Blast_Score=70, Evalue=4e-12,
Organism=Escherichia coli, GI1788683, Length=431, Percent_Identity=71.9257540603248, Blast_Score=635, Evalue=0.0,
Organism=Escherichia coli, GI87082165, Length=427, Percent_Identity=32.3185011709602, Blast_Score=196, Evalue=2e-51,
Organism=Escherichia coli, GI1788554, Length=422, Percent_Identity=33.8862559241706, Blast_Score=192, Evalue=3e-50,
Organism=Escherichia coli, GI48994986, Length=428, Percent_Identity=32.2429906542056, Blast_Score=186, Evalue=3e-48,
Organism=Escherichia coli, GI1787663, Length=425, Percent_Identity=30.8235294117647, Blast_Score=170, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI17551802, Length=443, Percent_Identity=37.9232505643341, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI133906874, Length=429, Percent_Identity=31.2354312354312, Blast_Score=179, Evalue=3e-45,
Organism=Caenorhabditis elegans, GI25147385, Length=421, Percent_Identity=27.790973871734, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI17535921, Length=423, Percent_Identity=27.8959810874704, Blast_Score=149, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6325229, Length=433, Percent_Identity=30.0230946882217, Blast_Score=170, Evalue=3e-43,
Organism=Saccharomyces cerevisiae, GI6322031, Length=450, Percent_Identity=27.3333333333333, Blast_Score=156, Evalue=8e-39,
Organism=Drosophila melanogaster, GI17137578, Length=445, Percent_Identity=41.123595505618, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24655093, Length=422, Percent_Identity=28.1990521327014, Blast_Score=169, Evalue=3e-42,
Organism=Drosophila melanogaster, GI24640423, Length=427, Percent_Identity=28.1030444964871, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI17648125, Length=381, Percent_Identity=27.5590551181102, Blast_Score=138, Evalue=8e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012806
- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.16 [H]

Molecular weight: Translated: 47609; Mature: 47478

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS00737 THIOLASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNSQRSSSSLSRHYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSSTPAVDLGKLAVKALM
CCCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
DKTEIDPKLIDQVVFGQVVQMPEAPNIAREIVLGTGMNVGTDAYSVTRACATSFQTTANV
HHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VESIMAGSIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVGQKLSLLRTLSLKDIA
HHHHHCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCC
PVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHSLAAKAWQDGLIQDEVMTA
CCCCHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FPDPYKEWIDQDNNIRYNSDLASYAKLRPAFDRKYGSVTAANSTPLTDGGAAILLMSEKR
CCCHHHHHCCCCCCEEECCHHHHHHHHCHHHHHCCCCEEECCCCCCCCCCCEEEEECCHH
AKELGYEPLGYIRSFAFSAMDVHHDMLMGPSYATPIALDKAGISLSDLTLIDMHEAFAAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHEEEHHHHHHHHHH
TLSNVKMFSSTKFAKECLGRDKAIGEIDMDKFNVLGGSLAYGHPFAATGARMIIQTLREL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KRRGGGFGLNTACAAGGLGAAMVLEVE
HHCCCCCCCHHHHHCCCCCEEEEEEEC
>Mature Secondary Structure 
SNSQRSSSSLSRHYQPLTTRQGDRIAVVSGIRTPFAKQSTAFSSTPAVDLGKLAVKALM
CCCCHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
DKTEIDPKLIDQVVFGQVVQMPEAPNIAREIVLGTGMNVGTDAYSVTRACATSFQTTANV
HHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
VESIMAGSIDIGIAGGADSSSVLPIGVSKKLASTLLALSKTKTVGQKLSLLRTLSLKDIA
HHHHHCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHCCC
PVPPAVAEYSTGISMGQTAEQMAKSHGITREEQDALAHRSHSLAAKAWQDGLIQDEVMTA
CCCCHHHHHHCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
FPDPYKEWIDQDNNIRYNSDLASYAKLRPAFDRKYGSVTAANSTPLTDGGAAILLMSEKR
CCCHHHHHCCCCCCEEECCHHHHHHHHCHHHHHCCCCEEECCCCCCCCCCCEEEEECCHH
AKELGYEPLGYIRSFAFSAMDVHHDMLMGPSYATPIALDKAGISLSDLTLIDMHEAFAAQ
HHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHEEEHHHHHHHHHH
TLSNVKMFSSTKFAKECLGRDKAIGEIDMDKFNVLGGSLAYGHPFAATGARMIIQTLREL
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHH
KRRGGGFGLNTACAAGGLGAAMVLEVE
HHCCCCCCCHHHHHCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA