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The map label for this gene is holB [H]

Identifier: 209695682

GI number: 209695682

Start: 2416217

End: 2417188

Strand: Direct

Name: holB [H]

Synonym: VSAL_I2246

Alternate gene names: 209695682

Gene position: 2416217-2417188 (Clockwise)

Preceding gene: 209695681

Following gene: 209695683

Centisome position: 72.66

GC content: 38.07

Gene sequence:

>972_bases
ATGGCAACTAAACTCTATCCTTGGCAGAAAGAGGTTTGGTCGCAATGGCAACAATTGATTGAAAAGGATCGATTGCCACA
CGCCATTTTATGCGCTATGCCTGATGGCGTTGGTCGAGACCGCCTTGCTCAATATTTTGCTGATACATTAGTTTGCTTGA
CACAAGGAACGGAACCTTGTGGTTTTTGCCATAGCTGTGGATTAATTGAATCAGGTAATCATCCTGATTTGCATTGGATC
AGACCTGAAGTTGAAGGAAAATCTATTTCTGTCGATCAAATTAGGCAGTGTAATCAATGGGCTTTAGAATCTTCTCAATT
TAATGCAAAACGCGTCATTATTATTGATCCTGCCGAAAAAATGACAGAGTCTGCCGCGAATGCTTTATTAAAGACATTAG
AAGCGCCCCCAAAGAACTGCCATTTTGTTTTACTTGCTAGTTCACCGCACAAACTATTACCGACGGTTCGGAGTCGATGT
CAGGTCTGGCATCAACCAAGTATCCCTGTTCCTGTTATCCGCGATTGGTTAATGGAGACTGAGCAATTAGATGTGACGCA
ACAATCGATTATAATAAATGGTTATTCGCCTTTACTTGTTCGTGGTTATTATCAGGACAAAAAAGTCATCCAGCATAAAC
AACTACTTGCTGAATTCGCGGTTTATGCGGACTCTTACTTTACCAATATTTCAGGAATATTACCTTTATTGGTTAAATTA
GGGGATGAAGGCTTATGTTGGTTAAGCTATTTATTATCTGATATACAAAAAGTACAGCTAGGAGTAGGCACTAACTTAGT
GCATTGTGATGAACTTACTTACGTTCAAAAACATGCAATTAAGCTCAACGCTGATTTAGTATACAAACAATATTTATCAT
TAAATGAGTTGCGACAACAGTTAAATAGTCATCCTGGATTGAATAAAGAATTATTAATTACTCAATGGTTATTTGAGTTT
ATTGGAGCGTAA

Upstream 100 bases:

>100_bases
GCTAATAATGACGAAAGTATTATAACCATTGATGCAGGACAAGACTTAGATACGGTAACAGCATCGATTATTGCCGCTTT
AGAAATATGGTTAGCGACGA

Downstream 100 bases:

>100_bases
TGTGTTAATTGATTCACACTGTCACCTTGATAAATTAAATTATGATGATCTGCATGTTAGTGTCGGTGATGTTATAGAAA
AAGCAAAACAACGTGGGGTT

Product: DNA polymerase III, delta' subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 322

Protein sequence:

>323_residues
MATKLYPWQKEVWSQWQQLIEKDRLPHAILCAMPDGVGRDRLAQYFADTLVCLTQGTEPCGFCHSCGLIESGNHPDLHWI
RPEVEGKSISVDQIRQCNQWALESSQFNAKRVIIIDPAEKMTESAANALLKTLEAPPKNCHFVLLASSPHKLLPTVRSRC
QVWHQPSIPVPVIRDWLMETEQLDVTQQSIIINGYSPLLVRGYYQDKKVIQHKQLLAEFAVYADSYFTNISGILPLLVKL
GDEGLCWLSYLLSDIQKVQLGVGTNLVHCDELTYVQKHAIKLNADLVYKQYLSLNELRQQLNSHPGLNKELLITQWLFEF
IGA

Sequences:

>Translated_323_residues
MATKLYPWQKEVWSQWQQLIEKDRLPHAILCAMPDGVGRDRLAQYFADTLVCLTQGTEPCGFCHSCGLIESGNHPDLHWI
RPEVEGKSISVDQIRQCNQWALESSQFNAKRVIIIDPAEKMTESAANALLKTLEAPPKNCHFVLLASSPHKLLPTVRSRC
QVWHQPSIPVPVIRDWLMETEQLDVTQQSIIINGYSPLLVRGYYQDKKVIQHKQLLAEFAVYADSYFTNISGILPLLVKL
GDEGLCWLSYLLSDIQKVQLGVGTNLVHCDELTYVQKHAIKLNADLVYKQYLSLNELRQQLNSHPGLNKELLITQWLFEF
IGA
>Mature_322_residues
ATKLYPWQKEVWSQWQQLIEKDRLPHAILCAMPDGVGRDRLAQYFADTLVCLTQGTEPCGFCHSCGLIESGNHPDLHWIR
PEVEGKSISVDQIRQCNQWALESSQFNAKRVIIIDPAEKMTESAANALLKTLEAPPKNCHFVLLASSPHKLLPTVRSRCQ
VWHQPSIPVPVIRDWLMETEQLDVTQQSIIINGYSPLLVRGYYQDKKVIQHKQLLAEFAVYADSYFTNISGILPLLVKLG
DEGLCWLSYLLSDIQKVQLGVGTNLVHCDELTYVQKHAIKLNADLVYKQYLSLNELRQQLNSHPGLNKELLITQWLFEFI
GA

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity [H]

COG id: COG0470

COG function: function code L; ATPase involved in DNA replication

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787341, Length=320, Percent_Identity=28.75, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1786676, Length=159, Percent_Identity=36.4779874213836, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR004622
- InterPro:   IPR015199 [H]

Pfam domain/function: PF09115 DNApol3-delta_C [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 36787; Mature: 36656

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATKLYPWQKEVWSQWQQLIEKDRLPHAILCAMPDGVGRDRLAQYFADTLVCLTQGTEPC
CCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
GFCHSCGLIESGNHPDLHWIRPEVEGKSISVDQIRQCNQWALESSQFNAKRVIIIDPAEK
CHHHHCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHH
MTESAANALLKTLEAPPKNCHFVLLASSPHKLLPTVRSRCQVWHQPSIPVPVIRDWLMET
HHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EQLDVTQQSIIINGYSPLLVRGYYQDKKVIQHKQLLAEFAVYADSYFTNISGILPLLVKL
HHCCCHHHEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDEGLCWLSYLLSDIQKVQLGVGTNLVHCDELTYVQKHAIKLNADLVYKQYLSLNELRQQ
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHEEECHHHHHHHHHHHHHHHHH
LNSHPGLNKELLITQWLFEFIGA
HHCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure 
ATKLYPWQKEVWSQWQQLIEKDRLPHAILCAMPDGVGRDRLAQYFADTLVCLTQGTEPC
CCCCCCHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
GFCHSCGLIESGNHPDLHWIRPEVEGKSISVDQIRQCNQWALESSQFNAKRVIIIDPAEK
CHHHHCCCCCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEEECCHHH
MTESAANALLKTLEAPPKNCHFVLLASSPHKLLPTVRSRCQVWHQPSIPVPVIRDWLMET
HHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHH
EQLDVTQQSIIINGYSPLLVRGYYQDKKVIQHKQLLAEFAVYADSYFTNISGILPLLVKL
HHCCCHHHEEEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GDEGLCWLSYLLSDIQKVQLGVGTNLVHCDELTYVQKHAIKLNADLVYKQYLSLNELRQQ
CCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHEEECHHHHHHHHHHHHHHHHH
LNSHPGLNKELLITQWLFEFIGA
HHCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]