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The map label for this gene is pyrF

Identifier: 209695661

GI number: 209695661

Start: 2391116

End: 2391820

Strand: Direct

Name: pyrF

Synonym: VSAL_I2225

Alternate gene names: 209695661

Gene position: 2391116-2391820 (Clockwise)

Preceding gene: 209695660

Following gene: 209695663

Centisome position: 71.91

GC content: 40.85

Gene sequence:

>705_bases
ATGAAAGACCAAAAGGTAATTGTGGCATTGGATTATGACCGTCAAGCTGACGCATTGGCGTTCGTGGATAAAATTGATCC
TAGCTCGTGCCGATTAAAAGTGGGTAAAGAGATGTTTACTCTTTTTGGCCCTGAGTTTGTAAAAGAACTTCATAAGCGTG
GTTTTTCTGTTTTTCTAGATTTAAAATTTCATGATATTCCTAATACGTGTTCAAAAGCAGTTCGTGCAGCTGCTGAAATG
GGGGTTTGGATGGTAAATGTTCATGCGAGTGGTGGTGAGCGGATGATGACAGCTTCGCGTGAGATTTTAGAACCGTATGG
CAAAGATCGCCCATTATTAATTGGTGTGACTGTTTTAACGAGCATGGAACAGCAAGATTTAGCGGGTATTGGTTTAGATA
TCGCACCACAAGAGCAAGTTAAACGTCTTGCTGCATTAACAAAAAACAGTGGTTTGGACGGAGTAGTTTGCTCAGCTCAA
GAAGCATCAATGCTAAAAGCTGACCTAGGTAAATACTTTAAGTTAGTAACACCTGGTATCCGTCCTGTGGGTTCAGACGT
TGGTGATCAAAAACGTATCATGACTCCAGTTGATGCGATTATTTCAGGATCTGATTACTTGGTAATTGGGCGCCCAATTA
CTCAAGCTGAAAACCCATCACAAGTATTAAATGACATTAATTTGTCGTTGGCTCCCGTCTTATAA

Upstream 100 bases:

>100_bases
GGGTGGGGGGTTATTAAGCCAATTAGAGGCTTAGACGGTGAATAATTAAGAGGCGCATGAGTGCCTCTTTTTTTGAATAA
AATAAAAATAAGGATAGAAA

Downstream 100 bases:

>100_bases
ATTGTTATGCAGATAAAAAAATAGAGACCTTATGGTCTCTATTTTTGTTTGTAAGCTCTCTATTAAAAGAGAAGAGTTAC
ATTACACGCATACCAGGTTG

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 234; Mature: 234

Protein sequence:

>234_residues
MKDQKVIVALDYDRQADALAFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEM
GVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLTSMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQ
EASMLKADLGKYFKLVTPGIRPVGSDVGDQKRIMTPVDAIISGSDYLVIGRPITQAENPSQVLNDINLSLAPVL

Sequences:

>Translated_234_residues
MKDQKVIVALDYDRQADALAFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEM
GVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLTSMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQ
EASMLKADLGKYFKLVTPGIRPVGSDVGDQKRIMTPVDAIISGSDYLVIGRPITQAENPSQVLNDINLSLAPVL
>Mature_234_residues
MKDQKVIVALDYDRQADALAFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLDLKFHDIPNTCSKAVRAAAEM
GVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLTSMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQ
EASMLKADLGKYFKLVTPGIRPVGSDVGDQKRIMTPVDAIISGSDYLVIGRPITQAENPSQVLNDINLSLAPVL

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=225, Percent_Identity=68.4444444444444, Blast_Score=316, Evalue=1e-87,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_ALISL (B6EIY4)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263590.1
- GeneID:   6987662
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2225
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- ProtClustDB:   PRK00230
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25474; Mature: 25474

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 120-120 BINDING 181-181 BINDING 190-190 BINDING 210-210 BINDING 211-211

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDQKVIVALDYDRQADALAFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLD
CCCCEEEEEECCCCCCCHHHHHHCCCCCHHEEHHHHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVRAAAEMGVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHH
SMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQEASMLKADLGKYFKLVTPGI
CCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCC
RPVGSDVGDQKRIMTPVDAIISGSDYLVIGRPITQAENPSQVLNDINLSLAPVL
CCCCCCCCCCCHHCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCEEECCC
>Mature Secondary Structure
MKDQKVIVALDYDRQADALAFVDKIDPSSCRLKVGKEMFTLFGPEFVKELHKRGFSVFLD
CCCCEEEEEECCCCCCCHHHHHHCCCCCHHEEHHHHHHHHHCCHHHHHHHHHCCCEEEEE
LKFHDIPNTCSKAVRAAAEMGVWMVNVHASGGERMMTASREILEPYGKDRPLLIGVTVLT
EEECCCCHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCEEEEEHHHH
SMEQQDLAGIGLDIAPQEQVKRLAALTKNSGLDGVVCSAQEASMLKADLGKYFKLVTPGI
CCCHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHHHHHCCCC
RPVGSDVGDQKRIMTPVDAIISGSDYLVIGRPITQAENPSQVLNDINLSLAPVL
CCCCCCCCCCCHHCCHHHHHHCCCCEEEECCCCCCCCCHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA