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The map label for this gene is aroA

Identifier: 209695655

GI number: 209695655

Start: 2385186

End: 2386469

Strand: Direct

Name: aroA

Synonym: VSAL_I2218

Alternate gene names: 209695655

Gene position: 2385186-2386469 (Clockwise)

Preceding gene: 209695651

Following gene: 209695656

Centisome position: 71.73

GC content: 39.41

Gene sequence:

>1284_bases
ATGGAAAGCTTAACTCTTCAACCAATTTCAAAAATTGATGGCCAAATTAATTTACCTGGTTCAAAAAGTGTTTCTAACCG
AGCACTATTACTTGCTGCACTAGCGTCAGGAAAAACAACATTAACTAATTTATTAGACAGCGATGATATTCGACATATGC
TTAATGCACTAAAAGCATTGGGTGTTGATTATAAACTTTCAGAAGATAAGACGGTTTGCGAAGTGAATGGTCTGGGTCAA
GCCTTTAAATCTACAACAGAAGCTTTAGAATTATTTCTGGGTAATGCGGGAACCGCAATGCGTCCATTAGCGGCTGCGTT
ATGTTTAGGTGAAGGCGAGTTTATCCTTACTGGTGAACCTCGTATGAAAGAGCGACCAATTGGGCACCTTGTTACTGCAT
TGAAAGCGGCTGGTGCTGATGTCGAATATTTAGAAAATGAAAATTATCCACCGTTAAAAATTAAAGGAACGGGTTTAAAA
GGCGGGAATGTTGATATAGATGGTTCAATATCAAGTCAATTTTTAACGGCTTTTTTAATGGCAGCACCACTATCATCACA
AGAAACAACCATTAATATTGTTGGCGATTTAGTGTCCAAACCGTATATCGATATTACGTTGGACATTATGGCTACTTTTG
GTGTTGTAATTGAAAACAAAGAGTATAAAACGTTTGTTGTTCCTGCGAATCAATCTTACATCGCCCCAGGAGAATTTCTT
GTAGAAGGAGATGCATCATCTGCATCTTATTTCTTAGCGGCTGCCGCGATCAAAGGCGGAAGTGTTAAAGTTACGGGTAT
TGGTAAAAAAAGCATCCAAGGCGATGTTCAGTTTGCTGATGCACTAGCAGCAATGGGTGCAGAGATTGAATGGGGTGATG
ACTATGTTATTGCTCATAAAGGTGAATTAAACGCCATTGATATGGATTTTAACCATATTCCAGATGCTGCAATGACAATC
GCAACGACAGCGTTGTTCGCAAAAGGCACTACATCAATTCGCAATGTGTATAATTGGCGTGTAAAAGAAACGGATAGATT
AGCCGCAATGGCAACTGAACTCCGTAAAGTAGGTGCTGAAGTTGAAGAAGGTGAGGATTATATTACGATCACTCCACCAG
CGATGTTACGACATGCCACTATTGATACTTATGACGATCACCGTATGGCAATGTGCTTTTCTTTAGTTGCATTAAGTGAT
ACGCCAGTGACGATTAATGATCCCGGTTGCACTTCTAAAACCTTCCCCGATTATTTTGATAAATTGAAAGAACTTAGCGT
GTAA

Upstream 100 bases:

>100_bases
TAACAAAAAATAACAATTAAAGCTGAAGCAGCTATGCAATATCTGAATCAAATAACAGGCATAGCATTATTTTTATTTGT
ATAGGAATTTTTACATAAAC

Downstream 100 bases:

>100_bases
TTTATTGTATTTAAGTCATTGGTTTTAAGTGGATGTAAGCCCTGTGTAAAAAATGCAGGGCTTTTTTAGTTTTTAAGAAA
CTAGAGTATATAAATAATCT

Product: 3-phosphoshikimate 1-carboxyvinyltransferase

Products: NA

Alternate protein names: 5-enolpyruvylshikimate-3-phosphate synthase; EPSP synthase; EPSPS

Number of amino acids: Translated: 427; Mature: 427

Protein sequence:

>427_residues
MESLTLQPISKIDGQINLPGSKSVSNRALLLAALASGKTTLTNLLDSDDIRHMLNALKALGVDYKLSEDKTVCEVNGLGQ
AFKSTTEALELFLGNAGTAMRPLAAALCLGEGEFILTGEPRMKERPIGHLVTALKAAGADVEYLENENYPPLKIKGTGLK
GGNVDIDGSISSQFLTAFLMAAPLSSQETTINIVGDLVSKPYIDITLDIMATFGVVIENKEYKTFVVPANQSYIAPGEFL
VEGDASSASYFLAAAAIKGGSVKVTGIGKKSIQGDVQFADALAAMGAEIEWGDDYVIAHKGELNAIDMDFNHIPDAAMTI
ATTALFAKGTTSIRNVYNWRVKETDRLAAMATELRKVGAEVEEGEDYITITPPAMLRHATIDTYDDHRMAMCFSLVALSD
TPVTINDPGCTSKTFPDYFDKLKELSV

Sequences:

>Translated_427_residues
MESLTLQPISKIDGQINLPGSKSVSNRALLLAALASGKTTLTNLLDSDDIRHMLNALKALGVDYKLSEDKTVCEVNGLGQ
AFKSTTEALELFLGNAGTAMRPLAAALCLGEGEFILTGEPRMKERPIGHLVTALKAAGADVEYLENENYPPLKIKGTGLK
GGNVDIDGSISSQFLTAFLMAAPLSSQETTINIVGDLVSKPYIDITLDIMATFGVVIENKEYKTFVVPANQSYIAPGEFL
VEGDASSASYFLAAAAIKGGSVKVTGIGKKSIQGDVQFADALAAMGAEIEWGDDYVIAHKGELNAIDMDFNHIPDAAMTI
ATTALFAKGTTSIRNVYNWRVKETDRLAAMATELRKVGAEVEEGEDYITITPPAMLRHATIDTYDDHRMAMCFSLVALSD
TPVTINDPGCTSKTFPDYFDKLKELSV
>Mature_427_residues
MESLTLQPISKIDGQINLPGSKSVSNRALLLAALASGKTTLTNLLDSDDIRHMLNALKALGVDYKLSEDKTVCEVNGLGQ
AFKSTTEALELFLGNAGTAMRPLAAALCLGEGEFILTGEPRMKERPIGHLVTALKAAGADVEYLENENYPPLKIKGTGLK
GGNVDIDGSISSQFLTAFLMAAPLSSQETTINIVGDLVSKPYIDITLDIMATFGVVIENKEYKTFVVPANQSYIAPGEFL
VEGDASSASYFLAAAAIKGGSVKVTGIGKKSIQGDVQFADALAAMGAEIEWGDDYVIAHKGELNAIDMDFNHIPDAAMTI
ATTALFAKGTTSIRNVYNWRVKETDRLAAMATELRKVGAEVEEGEDYITITPPAMLRHATIDTYDDHRMAMCFSLVALSD
TPVTINDPGCTSKTFPDYFDKLKELSV

Specific function: Aromatic amino acids biosynthesis; shikimate pathway; sixth step. [C]

COG id: COG0128

COG function: function code E; 5-enolpyruvylshikimate-3-phosphate synthase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EPSP synthase family

Homologues:

Organism=Escherichia coli, GI1787137, Length=426, Percent_Identity=74.6478873239437, Blast_Score=642, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320332, Length=446, Percent_Identity=37.8923766816144, Blast_Score=247, Evalue=2e-66,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): AROA_ALISL (B6EIX8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263584.1
- GeneID:   6988208
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I2218
- HOGENOM:   HBG646626
- OMA:   GADIEWG
- ProtClustDB:   PRK02427
- GO:   GO:0005737
- HAMAP:   MF_00210
- InterPro:   IPR001986
- InterPro:   IPR006264
- InterPro:   IPR023193
- InterPro:   IPR013792
- Gene3D:   G3DSA:3.65.10.10
- PIRSF:   PIRSF000505
- TIGRFAMs:   TIGR01356

Pfam domain/function: PF00275 EPSP_synthase; SSF55205 RNA3'_cycl/enolpyr_transf_A/B

EC number: =2.5.1.19

Molecular weight: Translated: 45750; Mature: 45750

Theoretical pI: Translated: 4.52; Mature: 4.52

Prosite motif: PS00104 EPSP_SYNTHASE_1; PS00885 EPSP_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESLTLQPISKIDGQINLPGSKSVSNRALLLAALASGKTTLTNLLDSDDIRHMLNALKAL
CCCCCCCCHHHCCCEEECCCCCCCCCCEEEEEEHHCCCHHHHHHHCCHHHHHHHHHHHHH
GVDYKLSEDKTVCEVNGLGQAFKSTTEALELFLGNAGTAMRPLAAALCLGEGEFILTGEP
CCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCEEEECCC
RMKERPIGHLVTALKAAGADVEYLENENYPPLKIKGTGLKGGNVDIDGSISSQFLTAFLM
CCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHH
AAPLSSQETTINIVGDLVSKPYIDITLDIMATFGVVIENKEYKTFVVPANQSYIAPGEFL
HCCCCCCCCEEEEEHHHHCCCEEEEEHHHHHHCCEEEECCCCEEEEEECCCCEECCCCEE
VEGDASSASYFLAAAAIKGGSVKVTGIGKKSIQGDVQFADALAAMGAEIEWGDDYVIAHK
EECCCCCCCEEEEEEEECCCCEEEEECCCHHCCCCHHHHHHHHHCCCEEEECCCEEEEEC
GELNAIDMDFNHIPDAAMTIATTALFAKGTTSIRNVYNWRVKETDRLAAMATELRKVGAE
CCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHEEEECCHHHHHHHHHHHHHHCCC
VEEGEDYITITPPAMLRHATIDTYDDHRMAMCFSLVALSDTPVTINDPGCTSKTFPDYFD
CCCCCCEEEECCHHHHHCCCCCCCCCCHHHHHHHHHEECCCCEEECCCCCCCCCCHHHHH
KLKELSV
HHHHCCC
>Mature Secondary Structure
MESLTLQPISKIDGQINLPGSKSVSNRALLLAALASGKTTLTNLLDSDDIRHMLNALKAL
CCCCCCCCHHHCCCEEECCCCCCCCCCEEEEEEHHCCCHHHHHHHCCHHHHHHHHHHHHH
GVDYKLSEDKTVCEVNGLGQAFKSTTEALELFLGNAGTAMRPLAAALCLGEGEFILTGEP
CCCEEECCCCEEEEECCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHEECCCCEEEECCC
RMKERPIGHLVTALKAAGADVEYLENENYPPLKIKGTGLKGGNVDIDGSISSQFLTAFLM
CCCCCCHHHHHHHHHHCCCCHHHHCCCCCCCEEEEECCCCCCCEEECCCHHHHHHHHHHH
AAPLSSQETTINIVGDLVSKPYIDITLDIMATFGVVIENKEYKTFVVPANQSYIAPGEFL
HCCCCCCCCEEEEEHHHHCCCEEEEEHHHHHHCCEEEECCCCEEEEEECCCCEECCCCEE
VEGDASSASYFLAAAAIKGGSVKVTGIGKKSIQGDVQFADALAAMGAEIEWGDDYVIAHK
EECCCCCCCEEEEEEEECCCCEEEEECCCHHCCCCHHHHHHHHHCCCEEEECCCEEEEEC
GELNAIDMDFNHIPDAAMTIATTALFAKGTTSIRNVYNWRVKETDRLAAMATELRKVGAE
CCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHEEEECCHHHHHHHHHHHHHHCCC
VEEGEDYITITPPAMLRHATIDTYDDHRMAMCFSLVALSDTPVTINDPGCTSKTFPDYFD
CCCCCCEEEECCHHHHHCCCCCCCCCCHHHHHHHHHEECCCCEEECCCCCCCCCCHHHHH
KLKELSV
HHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA