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The map label for this gene is cspD [H]

Identifier: 209695649

GI number: 209695649

Start: 2379927

End: 2380148

Strand: Reverse

Name: cspD [H]

Synonym: VSAL_I2212

Alternate gene names: 209695649

Gene position: 2380148-2379927 (Counterclockwise)

Preceding gene: 209695652

Following gene: 209695648

Centisome position: 71.58

GC content: 41.89

Gene sequence:

>222_bases
ATGGCAATAGGCACAGTAAAATGGTTCAATAACGCCAAAGGGTTTGGCTTCATTTGTCCAGAAGGTGAGGATGGTGATAT
TTTTGCTCATTACTCAACAATCGAGATGGATGGCTACCGTACATTAAAAGCAGGACAAGCTGTCACCTACGAAATTCATG
AAGGCCCTAAAGGCTATCATGCAAGCGAAATTAAACCGCTTGAAGGTGAAGCTGTAAAATAG

Upstream 100 bases:

>100_bases
TATATTTAATAGAGTGTACCTAGTAGTGGTTATCAATTAGGCAAAAACTTAATTGATAGACATTATGCTAGGTAACTAAA
AATGCAGAGGGATGTATAGC

Downstream 100 bases:

>100_bases
CATAAAATAATACTTACTGTTAAAGTGAGATCAACGGTGAGTATTACTACTCACCTTTTTTGAACCTATCACATAAGAAA
TACTCTAAACTCCAATCAAT

Product: cold shock-like protein CspD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 73; Mature: 72

Protein sequence:

>73_residues
MAIGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMDGYRTLKAGQAVTYEIHEGPKGYHASEIKPLEGEAVK

Sequences:

>Translated_73_residues
MAIGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMDGYRTLKAGQAVTYEIHEGPKGYHASEIKPLEGEAVK
>Mature_72_residues
AIGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMDGYRTLKAGQAVTYEIHEGPKGYHASEIKPLEGEAVK

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=71, Percent_Identity=76.056338028169, Blast_Score=116, Evalue=2e-28,
Organism=Escherichia coli, GI1786841, Length=64, Percent_Identity=50, Blast_Score=73, Evalue=4e-15,
Organism=Escherichia coli, GI1789979, Length=64, Percent_Identity=48.4375, Blast_Score=70, Evalue=3e-14,
Organism=Escherichia coli, GI1787839, Length=61, Percent_Identity=52.4590163934426, Blast_Score=69, Evalue=4e-14,
Organism=Escherichia coli, GI1788126, Length=64, Percent_Identity=46.875, Blast_Score=69, Evalue=7e-14,
Organism=Escherichia coli, GI2367114, Length=64, Percent_Identity=42.1875, Blast_Score=64, Evalue=2e-12,
Organism=Escherichia coli, GI1787834, Length=64, Percent_Identity=45.3125, Blast_Score=63, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 8023; Mature: 7892

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00352 COLD_SHOCK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMDGYRTLKAGQAVTYEIHEGPKGYH
CCEEEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEECCCCCCCC
ASEIKPLEGEAVK
CCCCCCCCCCCCC
>Mature Secondary Structure 
AIGTVKWFNNAKGFGFICPEGEDGDIFAHYSTIEMDGYRTLKAGQAVTYEIHEGPKGYH
CEEEEEECCCCCCCEEECCCCCCCCEEEEEEEEEECCCEEECCCCEEEEEECCCCCCCC
ASEIKPLEGEAVK
CCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]