Click here to switch to the map view.

The map label for this gene is purB [H]

Identifier: 209695632

GI number: 209695632

Start: 2358786

End: 2360156

Strand: Reverse

Name: purB [H]

Synonym: VSAL_I2195

Alternate gene names: 209695632

Gene position: 2360156-2358786 (Counterclockwise)

Preceding gene: 209695633

Following gene: 209695630

Centisome position: 70.98

GC content: 42.6

Gene sequence:

>1371_bases
ATGGAATTGTCAGCATTAACTGCTGTTTCACCTGTAGACGGTCGCTACGGAAGTAAAACAATTTCTCTACGCAGCATCTT
TAGTGAGTATGGCTTACTGAAATACCGTACTGTTGTTGAAGTTCGTTGGCTTCAAAAATTAGCAGCTACGCAATCGATTG
CGGAAGTGGCTACATTAAGCGCAGAAGCGAATCAATTCTTAGATAACATCGCTGCAAACTTCAGCGAAGAAGACGCTATG
CGTATCAAGGACATTGAACGCACAACAAACCACGATGTTAAAGCCGTTGAATATTTCCTAAAAGAGAAAGTCGCAGAGCT
TCCAGAGCTTCACGCTATCAACGAATTTATTCACTTTGCTTGTACTTCAGAAGACATAAACAATACGTCTCACGCTCTTA
TGCTTAAAGAAGCACGTGACACGATCGTTCTTCCTGAAATCCGTAACGTTATCGATGCAATTAAAGCATTAGCGAACGAG
TACCGTGATATTCCTCTTCTTTCTCGTACACACGGTCAGCCAGCTTCACCTTCTACAATGGGTAAAGAAATGGCGAATGT
TGCGTACCGTATGGAACGTCAATACAAACAAATCGAAAACGTTGAGATCCTAGCGAAAATCAACGGTGCTGTTGGTAACT
ACAACGCTCACCTTTCAGCTTACCCAGATGTTGATTGGCACAAATTCAGTGAAGAATTCATCACTGAATCTCTAGGCGTT
AACTGGAATCCGTACACAACTCAAATCGAACCGCATGACTACATTGCAGAGCTATTTGATGCGATTGCACGTTTCAACAC
AATCCTTCTTGATTTTGACCGTGACGTTTGGGGCTACATTGCTCTTGGTCACTTCAAACAAAAAACGATTGCAGGCGAAA
TCGGCTCTTCAACTATGCCGCATAAAGTTAACCCAATCGATTTTGAAAACTCTGAAGGTAACCTAGGTCTTGCTAACGCT
ATCTTTAATCACCTATCTCAAAAACTGCCTGTATCTCGCTGGCAGCGTGACCTAACTGACTCTACGGTTCTTCGTAATCT
AGGTGTTGGTTGTGGTTACGCAATCATTGCATACACGTCGACTCTAAAAGGCATTAGCAAATTAGAAATCAATCGTGCAG
CACTTGAAGCTGAGCTAGATAAAAACTGGGAAGTTCTTGCAGAACCGGTTCAAACAGTAATGCGTCGTTACGGCATCGAA
AAGCCATACGAAAAACTAAAAGAGCTAACTCGCGGTAAACGCATTGATGGTGAAGGCATGCGTTCATTCATCGACAGCTT
AGAAATCCCTGCAGACGAAAAAGTTCGTTTAAAAGAGATGACGCCAGCAAACTACATTGGTCAAGCTATCGAGCTAGCTG
ACAAGCTTTAA

Upstream 100 bases:

>100_bases
CAAGCAAAAATTATATTAGCTCGAATTTAAAAAATTAAAGCCTACTTGACTATCAGGTAGGCTTTATCCTTTAACCTTTA
TAGATATCTAGGAGATTCAC

Downstream 100 bases:

>100_bases
TCTTTACGGTTATTTATTAACCTAACAGATTTATAAGCACTAAAGAGCCCCGTATCAAACGCCTTTAAAGAGCGATTGTT
ACAGGGCTTTTTTATTTAGT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQSIAEVATLSAEANQFLDNIAANFSEEDAM
RIKDIERTTNHDVKAVEYFLKEKVAELPELHAINEFIHFACTSEDINNTSHALMLKEARDTIVLPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
NWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
IFNHLSQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTSTLKGISKLEINRAALEAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRIDGEGMRSFIDSLEIPADEKVRLKEMTPANYIGQAIELADKL

Sequences:

>Translated_456_residues
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQSIAEVATLSAEANQFLDNIAANFSEEDAM
RIKDIERTTNHDVKAVEYFLKEKVAELPELHAINEFIHFACTSEDINNTSHALMLKEARDTIVLPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
NWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
IFNHLSQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTSTLKGISKLEINRAALEAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRIDGEGMRSFIDSLEIPADEKVRLKEMTPANYIGQAIELADKL
>Mature_456_residues
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQSIAEVATLSAEANQFLDNIAANFSEEDAM
RIKDIERTTNHDVKAVEYFLKEKVAELPELHAINEFIHFACTSEDINNTSHALMLKEARDTIVLPEIRNVIDAIKALANE
YRDIPLLSRTHGQPASPSTMGKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
NWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLANA
IFNHLSQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTSTLKGISKLEINRAALEAELDKNWEVLAEPVQTVMRRYGIE
KPYEKLKELTRGKRIDGEGMRSFIDSLEIPADEKVRLKEMTPANYIGQAIELADKL

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=456, Percent_Identity=79.3859649122807, Blast_Score=770, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6323391, Length=346, Percent_Identity=23.9884393063584, Blast_Score=80, Evalue=7e-16,
Organism=Drosophila melanogaster, GI24647570, Length=348, Percent_Identity=25.2873563218391, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 51446; Mature: 51446

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQSIAEVATLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEANQFLDNIAANFSEEDAMRIKDIERTTNHDVKAVEYFLKEKVAELPELHAINEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTIVLPEIRNVIDAIKALANEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
NWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAIFNHLSQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLKGISKLEINRAALEAELDKNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDGEGM
HHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
RSFIDSLEIPADEKVRLKEMTPANYIGQAIELADKL
HHHHHHCCCCCCCCEEHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MELSALTAVSPVDGRYGSKTISLRSIFSEYGLLKYRTVVEVRWLQKLAATQSIAEVATLS
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AEANQFLDNIAANFSEEDAMRIKDIERTTNHDVKAVEYFLKEKVAELPELHAINEFIHFA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
CTSEDINNTSHALMLKEARDTIVLPEIRNVIDAIKALANEYRDIPLLSRTHGQPASPSTM
CCCCCCCCCHHHEEEECCCCCEECHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCHHH
GKEMANVAYRMERQYKQIENVEILAKINGAVGNYNAHLSAYPDVDWHKFSEEFITESLGV
HHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCCCCCEEECCCCHHHHHHHHHHHHHCCC
NWNPYTTQIEPHDYIAELFDAIARFNTILLDFDRDVWGYIALGHFKQKTIAGEIGSSTMP
CCCCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCC
HKVNPIDFENSEGNLGLANAIFNHLSQKLPVSRWQRDLTDSTVLRNLGVGCGYAIIAYTS
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TLKGISKLEINRAALEAELDKNWEVLAEPVQTVMRRYGIEKPYEKLKELTRGKRIDGEGM
HHCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCHHHH
RSFIDSLEIPADEKVRLKEMTPANYIGQAIELADKL
HHHHHHCCCCCCCCEEHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]