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The map label for this gene is helD [H]

Identifier: 209695575

GI number: 209695575

Start: 2282975

End: 2285038

Strand: Reverse

Name: helD [H]

Synonym: VSAL_I2134

Alternate gene names: 209695575

Gene position: 2285038-2282975 (Counterclockwise)

Preceding gene: 209695577

Following gene: 209695572

Centisome position: 68.72

GC content: 39.58

Gene sequence:

>2064_bases
ATGCAGATTAAAGCAACCACCTTCGCTCAATGGTTAGTTCAGGGTGACTTTTATCAACTTGAATTAGAGCCAGAGCAGCT
GTTGTTTACCTCCCACAAATATCAAGTTTCTGTGCCGTTTGATAAATGGAACGGGCAAGTTACTTTCGAACGAGGATTAG
TGTGGGGAGCGCTTTGTTTCTATAATCGAAATGAACAAATAGCGTGGCGAGTTTCTGGGTTGCCTTGGCAAGAGTGCGAC
GCGGTAATTAATGCCGTATTAAACGCGTACGCAGAATGGAAAGAATCGAAAGTCGTTATGTTGAATCAGTTACAACCCCA
GATGTTAGAACTGGTGAGGACGTTCAGTGCACAGCGTCGTTATTTGAAGCAAACCGAAGCGCTGTTGATGGTTGAGAAAT
TATACGATTTATTTGAGCAAACCAAGATATCGATCCCTTTAGCTCAACAACTTCAGCCTGAATGTATTGCGCCTGTATTA
GAGTGGTTAATGGAACCTGAACAACAACTTATCACGTTAAACGAAACGTGGTTAGAAGAGCAAAAAGTGGAGTGGAAATC
TTTTTTCTCAGAATGTGAATCACAACCATTAAACGAATCACAGCAAAGTGCGCTGTTATTAAATGAAGACCATACATTAG
TATTAGCGGGAGCTGGCTCAGGTAAAACAAGTGTACTTGTTGCTCGTGTTAATTATTTGATTAAAAGCCAAGTGGCTCAA
CCTGAAGAGATTTTATTATTGGCTTTTGGTCGTCAGGCCGCTCAAGAAATGTCAGCTCGGATCATTGAGAAGCTAGGAAC
TGATATTGGACAAAAAGTAAAAGTGGCCACGTTTCACCAATTAGGGTTGGATATTATTCGTCATGTCGAATACCAACAAC
CCAAATTGTCATGCTTGGTGACGGATTCAAAAGCAAGAAAAGAGTGGTTTAGCCATGTGTTAGATTCGGAGTGGAAAAAT
GCGACAGCAACGAATCGTTGGAAAAAACACTTAAAAAAATGGCCTATCGCTTACTTACGTGGTGATGTTGATTTAAAAGA
AGAATCGCATAACGAAAAATTACAAGAATGGCTGCACAAACAAGTATGTCAGTTAAACAGCTTACAGCTAGATAAAAAAA
CAGTTACCGCCCGTATTGAAAACCATGCTGATGCGGCGCGTTTAAAAAGCGAGCTCAACATTGTATGGCCATTTTTTAAA
GCGTATGAAAGAACCCTTAAACAAGAAGACGCAATTGATTATGATTTAATGATAACCAAAGCGACAGCCTATATAAATGA
GGGGAAATACGCCTTACCGTGGTCATTTGTTATGGTTGATGAATATCAAGATATCTCTCCGCAGCGTTTAGATTTAATTG
AAGCTATTTGCCATAAAGAGAATGATAAAAAAGAAAGAGCCACATTATTTGCGGTCGGTGATGACTGGCAAGCGATTTAT
CGTTTTGCTGGTGCAGATGTATCTCTAACTACTGGGTTCGAACAACGTTTTCAATCTTGTCAAATTGCAAATTTATCAAT
GACGTATCGTTTTAATGATCAAATTGGTGCCGTTGCTAACCGTTTTGTACAAGAAAACCCGATGCAGCTTAAAAAAGAGC
TGAACAGTTTCACAAAACAAAAACGTAAAGCAGTGAAAGTGATTGAGCACCAATCGATAGAAAAAGAATTACTCTCTTTG
TCTAAAAATATTCGTGATGGTGAGAAGGTTCTGATGTTAGGACGTAATTATAATCACTGTCCTGATGCTTTTGATGAGTG
GGTGAAATTTTACCCAGGGTTAAACCTTGAATACAAAACGTGTCATGCAAGTAAAGGAACTGAAGCTGATTATGTCTTTA
TTGTTGAAATGACGGATGGTCAATTCCCAATGAAAGAGAGAGCGGAATCTTTAGATGGCGCATTGCTGCCACACCTTGAA
GATTTTGCTTATGCTGAAGAGCGTCGATTGTTTTATGTTGCGTTAACGCGAGCGAAGAAGAAAGTGTGGATAGTATATCA
TGGAAAGGCATCCCCATTTGTTCAAGAGATTGTCGATAAAGATTACCCCGTGATCCTTGAGTGA

Upstream 100 bases:

>100_bases
ACAACAAAAAAATAACCTGATATCCTATGCGGTCTTCGGTTGACGTCGAGTTGCCGAAAATACGTTCATCAGTGTAATTT
TTATTAGGTAGTATCGAATC

Downstream 100 bases:

>100_bases
TGATGGATAGACACTGATAAATAAAAAGCCCTTATTATATTGTTAATAAGGGCTTTTTAAATGAATCGCACTTCTTAATT
TATTGTATCTATTAGATGCG

Product: DNA helicase IV

Products: NA

Alternate protein names: 75 kDa helicase [H]

Number of amino acids: Translated: 687; Mature: 687

Protein sequence:

>687_residues
MQIKATTFAQWLVQGDFYQLELEPEQLLFTSHKYQVSVPFDKWNGQVTFERGLVWGALCFYNRNEQIAWRVSGLPWQECD
AVINAVLNAYAEWKESKVVMLNQLQPQMLELVRTFSAQRRYLKQTEALLMVEKLYDLFEQTKISIPLAQQLQPECIAPVL
EWLMEPEQQLITLNETWLEEQKVEWKSFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIKSQVAQ
PEEILLLAFGRQAAQEMSARIIEKLGTDIGQKVKVATFHQLGLDIIRHVEYQQPKLSCLVTDSKARKEWFSHVLDSEWKN
ATATNRWKKHLKKWPIAYLRGDVDLKEESHNEKLQEWLHKQVCQLNSLQLDKKTVTARIENHADAARLKSELNIVWPFFK
AYERTLKQEDAIDYDLMITKATAYINEGKYALPWSFVMVDEYQDISPQRLDLIEAICHKENDKKERATLFAVGDDWQAIY
RFAGADVSLTTGFEQRFQSCQIANLSMTYRFNDQIGAVANRFVQENPMQLKKELNSFTKQKRKAVKVIEHQSIEKELLSL
SKNIRDGEKVLMLGRNYNHCPDAFDEWVKFYPGLNLEYKTCHASKGTEADYVFIVEMTDGQFPMKERAESLDGALLPHLE
DFAYAEERRLFYVALTRAKKKVWIVYHGKASPFVQEIVDKDYPVILE

Sequences:

>Translated_687_residues
MQIKATTFAQWLVQGDFYQLELEPEQLLFTSHKYQVSVPFDKWNGQVTFERGLVWGALCFYNRNEQIAWRVSGLPWQECD
AVINAVLNAYAEWKESKVVMLNQLQPQMLELVRTFSAQRRYLKQTEALLMVEKLYDLFEQTKISIPLAQQLQPECIAPVL
EWLMEPEQQLITLNETWLEEQKVEWKSFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIKSQVAQ
PEEILLLAFGRQAAQEMSARIIEKLGTDIGQKVKVATFHQLGLDIIRHVEYQQPKLSCLVTDSKARKEWFSHVLDSEWKN
ATATNRWKKHLKKWPIAYLRGDVDLKEESHNEKLQEWLHKQVCQLNSLQLDKKTVTARIENHADAARLKSELNIVWPFFK
AYERTLKQEDAIDYDLMITKATAYINEGKYALPWSFVMVDEYQDISPQRLDLIEAICHKENDKKERATLFAVGDDWQAIY
RFAGADVSLTTGFEQRFQSCQIANLSMTYRFNDQIGAVANRFVQENPMQLKKELNSFTKQKRKAVKVIEHQSIEKELLSL
SKNIRDGEKVLMLGRNYNHCPDAFDEWVKFYPGLNLEYKTCHASKGTEADYVFIVEMTDGQFPMKERAESLDGALLPHLE
DFAYAEERRLFYVALTRAKKKVWIVYHGKASPFVQEIVDKDYPVILE
>Mature_687_residues
MQIKATTFAQWLVQGDFYQLELEPEQLLFTSHKYQVSVPFDKWNGQVTFERGLVWGALCFYNRNEQIAWRVSGLPWQECD
AVINAVLNAYAEWKESKVVMLNQLQPQMLELVRTFSAQRRYLKQTEALLMVEKLYDLFEQTKISIPLAQQLQPECIAPVL
EWLMEPEQQLITLNETWLEEQKVEWKSFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIKSQVAQ
PEEILLLAFGRQAAQEMSARIIEKLGTDIGQKVKVATFHQLGLDIIRHVEYQQPKLSCLVTDSKARKEWFSHVLDSEWKN
ATATNRWKKHLKKWPIAYLRGDVDLKEESHNEKLQEWLHKQVCQLNSLQLDKKTVTARIENHADAARLKSELNIVWPFFK
AYERTLKQEDAIDYDLMITKATAYINEGKYALPWSFVMVDEYQDISPQRLDLIEAICHKENDKKERATLFAVGDDWQAIY
RFAGADVSLTTGFEQRFQSCQIANLSMTYRFNDQIGAVANRFVQENPMQLKKELNSFTKQKRKAVKVIEHQSIEKELLSL
SKNIRDGEKVLMLGRNYNHCPDAFDEWVKFYPGLNLEYKTCHASKGTEADYVFIVEMTDGQFPMKERAESLDGALLPHLE
DFAYAEERRLFYVALTRAKKKVWIVYHGKASPFVQEIVDKDYPVILE

Specific function: Helicase IV catalyzes the unwinding of duplex DNA in the 3' to 5' direction with respect to the bound single strand in a reaction that is dependent upon the hydrolysis of ATP [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase ATP-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787196, Length=701, Percent_Identity=35.09272467903, Blast_Score=396, Evalue=1e-111,
Organism=Escherichia coli, GI2367296, Length=346, Percent_Identity=25.7225433526012, Blast_Score=86, Evalue=1e-17,
Organism=Escherichia coli, GI48994965, Length=91, Percent_Identity=41.7582417582418, Blast_Score=73, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000212
- InterPro:   IPR014016
- InterPro:   IPR022161
- InterPro:   IPR003661 [H]

Pfam domain/function: PF12462 Nucleolin_N; PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 79784; Mature: 79784

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIKATTFAQWLVQGDFYQLELEPEQLLFTSHKYQVSVPFDKWNGQVTFERGLVWGALCF
CCCCHHHHHHHHHCCCEEEEEECHHHHEEECCCEEEEECHHCCCCEEEEECCCEEEEHEE
YNRNEQIAWRVSGLPWQECDAVINAVLNAYAEWKESKVVMLNQLQPQMLELVRTFSAQRR
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
YLKQTEALLMVEKLYDLFEQTKISIPLAQQLQPECIAPVLEWLMEPEQQLITLNETWLEE
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCHHEEEECHHHHHH
QKVEWKSFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIKSQVAQ
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
PEEILLLAFGRQAAQEMSARIIEKLGTDIGQKVKVATFHQLGLDIIRHVEYQQPKLSCLV
CHHEEEEHHCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEE
TDSKARKEWFSHVLDSEWKNATATNRWKKHLKKWPIAYLRGDVDLKEESHNEKLQEWLHK
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHH
QVCQLNSLQLDKKTVTARIENHADAARLKSELNIVWPFFKAYERTLKQEDAIDYDLMITK
HHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCEEEEEEE
ATAYINEGKYALPWSFVMVDEYQDISPQRLDLIEAICHKENDKKERATLFAVGDDWQAIY
EEEEECCCCEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHH
RFAGADVSLTTGFEQRFQSCQIANLSMTYRFNDQIGAVANRFVQENPMQLKKELNSFTKQ
HHCCCCEEEECCHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHH
KRKAVKVIEHQSIEKELLSLSKNIRDGEKVLMLGRNYNHCPDAFDEWVKFYPGLNLEYKT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEE
CHASKGTEADYVFIVEMTDGQFPMKERAESLDGALLPHLEDFAYAEERRLFYVALTRAKK
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEEECCCC
KVWIVYHGKASPFVQEIVDKDYPVILE
EEEEEEECCCCHHHHHHHCCCCCEEEC
>Mature Secondary Structure
MQIKATTFAQWLVQGDFYQLELEPEQLLFTSHKYQVSVPFDKWNGQVTFERGLVWGALCF
CCCCHHHHHHHHHCCCEEEEEECHHHHEEECCCEEEEECHHCCCCEEEEECCCEEEEHEE
YNRNEQIAWRVSGLPWQECDAVINAVLNAYAEWKESKVVMLNQLQPQMLELVRTFSAQRR
ECCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHHHHH
YLKQTEALLMVEKLYDLFEQTKISIPLAQQLQPECIAPVLEWLMEPEQQLITLNETWLEE
HHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCHHHHHHHHHHHHCCCHHEEEECHHHHHH
QKVEWKSFFSECESQPLNESQQSALLLNEDHTLVLAGAGSGKTSVLVARVNYLIKSQVAQ
HHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHCCC
PEEILLLAFGRQAAQEMSARIIEKLGTDIGQKVKVATFHQLGLDIIRHVEYQQPKLSCLV
CHHEEEEHHCHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHCCCCCCCEEEEE
TDSKARKEWFSHVLDSEWKNATATNRWKKHLKKWPIAYLRGDVDLKEESHNEKLQEWLHK
ECCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHEEECCCCCCCCHHHHHHHHHHHH
QVCQLNSLQLDKKTVTARIENHADAARLKSELNIVWPFFKAYERTLKQEDAIDYDLMITK
HHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHCCEEHHHHHHHHHHHHHCCCCCEEEEEEE
ATAYINEGKYALPWSFVMVDEYQDISPQRLDLIEAICHKENDKKERATLFAVGDDWQAIY
EEEEECCCCEECCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEEEECCCHHHHH
RFAGADVSLTTGFEQRFQSCQIANLSMTYRFNDQIGAVANRFVQENPMQLKKELNSFTKQ
HHCCCCEEEECCHHHHHCCCEEEEEEEEEEECCHHHHHHHHHHHCCHHHHHHHHHHHHHH
KRKAVKVIEHQSIEKELLSLSKNIRDGEKVLMLGRNYNHCPDAFDEWVKFYPGLNLEYKT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHCCCCCCEEEE
CHASKGTEADYVFIVEMTDGQFPMKERAESLDGALLPHLEDFAYAEERRLFYVALTRAKK
ECCCCCCCCCEEEEEEECCCCCCHHHHHHHCCCCCCCCHHHHHHHHHCEEEEEEEECCCC
KVWIVYHGKASPFVQEIVDKDYPVILE
EEEEEEECCCCHHHHHHHCCCCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2542273; 8905232; 9278503 [H]