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The map label for this gene is dcuA [H]

Identifier: 209695462

GI number: 209695462

Start: 2146411

End: 2147712

Strand: Reverse

Name: dcuA [H]

Synonym: VSAL_I2005

Alternate gene names: 209695462

Gene position: 2147712-2146411 (Counterclockwise)

Preceding gene: 209695464

Following gene: 209695461

Centisome position: 64.59

GC content: 42.47

Gene sequence:

>1302_bases
ATGTCGATAATTATCGAAATATCTGTTGTTTTAATCTGTATATACTGGGGCTTAAAAATGGGAGGCCTTGGTGTTGGTTT
AGCGGGTGGACTTGGGCTCGCGATACTCACTCTTGGATTAGGTCTTGAACCTAGTGCTCCTGCTATCGATGTTATTTTGA
TCATCATGTCAGTAGTAACAGCAGCATCAACAATGCAAGCGGCCGGAGGTATGGATTGGTTGGTGCATCATGCCGGAAAC
TTAATGCGCCGCCACCCAAAATATATCAACGTATTAGCGCCTATGATCACTTGGTGTATGACGATTATGGCTGGTACTGG
TTACACTGTTTTTTCTACACTTCCTGTTATAGCAGAGGTTGCAAAAGAGATAGGGGAACGTCCATCAAGACCACTGTCAG
CTGCAGTTGTTGCATCCCAAGTTGCTATTTCTGGCTCACCAATTTCAGCAGCAATGGCCGCTATGATTTCAATAATGGAA
CCTGCTGGCGTTAGTTTTTTACAAATAATGGCGATTTGTTTACCTGCCTCTTTTATTGCCGCAATGGTTGCTGCTTTTGT
TGCTTCTCGCCAAGGCTGTGAGTTAAAAGATGATGTGATATATCAACAAATTCAAAAAAATAACAAGCAACAAACAACGA
AAAAATCAGAAGCTATCGATCCAAAAGCAAAATATTCGGTCATCTTCTTTGCATTAGCAACGTGTGCCATTGTTATTTAT
GCGGCGATTCCATCACTTCGCCCCGCCTTTCCAAGTGGTAATATTATGTCAACACGAGACATCATTATTGTCAGTATGTT
TGCAGCGGCCTGTTTGATGGTACCAATGTGTAACATTCACCCTGACCGCATTATCCGTACCACCACATTCCGTTCAGGCA
TGAGCTCTGTTGCCGTGATATTAGGTATTGTGACTATGGGATCAACCGTTGTTAATGCACACCTTCCTGAAATAAAAGAA
TTTGCAGGGTCTCTTCTTCATTCTTATCCAATGCTGTTATCTCTTGTTCTTTTTGGTACTTGTACGCTTCTAATGTCACA
AGGAGCAACCACTCCATTAATTGTTCCGTTAGCACTGAGTTTAAACGTTCCTCATTGGGCAATCTTGGCATCATTTGTTG
CCGTTACGGGTGTTTTTGTTCTGCCTACTTATCCTACATCTCTTGCTGCCATTGAGTTTGACTCAACAGGATCTACACAC
ATGGGAAAAAACATTTTCGACCATCCATTCATGCTACCCGGCTTAACAGGGGTGGTTGTCGCCATTGGATTCGGATTCTT
ACTTGCTCCTATCGTTCTTTAA

Upstream 100 bases:

>100_bases
CATTTAACAACCTTCAGCCTAACTGACATGATTTATTCAGCTTATTCATCATTGATGATGAATAAAAAATTTAATTATTT
AGTCTGAAATAGGATGTTGT

Downstream 100 bases:

>100_bases
GTCTCATAAATACATATATTAAAGGAATCATATTATGAATAAAAAATTAGGTATTTTAGGTGGCATGGGGCCATTGGCAA
CGGTTGAATTTATGATGAAA

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 432

Protein sequence:

>433_residues
MSIIIEISVVLICIYWGLKMGGLGVGLAGGLGLAILTLGLGLEPSAPAIDVILIIMSVVTAASTMQAAGGMDWLVHHAGN
LMRRHPKYINVLAPMITWCMTIMAGTGYTVFSTLPVIAEVAKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIME
PAGVSFLQIMAICLPASFIAAMVAAFVASRQGCELKDDVIYQQIQKNNKQQTTKKSEAIDPKAKYSVIFFALATCAIVIY
AAIPSLRPAFPSGNIMSTRDIIIVSMFAAACLMVPMCNIHPDRIIRTTTFRSGMSSVAVILGIVTMGSTVVNAHLPEIKE
FAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALSLNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTH
MGKNIFDHPFMLPGLTGVVVAIGFGFLLAPIVL

Sequences:

>Translated_433_residues
MSIIIEISVVLICIYWGLKMGGLGVGLAGGLGLAILTLGLGLEPSAPAIDVILIIMSVVTAASTMQAAGGMDWLVHHAGN
LMRRHPKYINVLAPMITWCMTIMAGTGYTVFSTLPVIAEVAKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIME
PAGVSFLQIMAICLPASFIAAMVAAFVASRQGCELKDDVIYQQIQKNNKQQTTKKSEAIDPKAKYSVIFFALATCAIVIY
AAIPSLRPAFPSGNIMSTRDIIIVSMFAAACLMVPMCNIHPDRIIRTTTFRSGMSSVAVILGIVTMGSTVVNAHLPEIKE
FAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALSLNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTH
MGKNIFDHPFMLPGLTGVVVAIGFGFLLAPIVL
>Mature_432_residues
SIIIEISVVLICIYWGLKMGGLGVGLAGGLGLAILTLGLGLEPSAPAIDVILIIMSVVTAASTMQAAGGMDWLVHHAGNL
MRRHPKYINVLAPMITWCMTIMAGTGYTVFSTLPVIAEVAKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIMEP
AGVSFLQIMAICLPASFIAAMVAAFVASRQGCELKDDVIYQQIQKNNKQQTTKKSEAIDPKAKYSVIFFALATCAIVIYA
AIPSLRPAFPSGNIMSTRDIIIVSMFAAACLMVPMCNIHPDRIIRTTTFRSGMSSVAVILGIVTMGSTVVNAHLPEIKEF
AGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALSLNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTHM
GKNIFDHPFMLPGLTGVVVAIGFGFLLAPIVL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=433, Percent_Identity=42.9561200923788, Blast_Score=332, Evalue=2e-92,
Organism=Escherichia coli, GI1790565, Length=400, Percent_Identity=34, Blast_Score=235, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 45623; Mature: 45492

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
7.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIIEISVVLICIYWGLKMGGLGVGLAGGLGLAILTLGLGLEPSAPAIDVILIIMSVVT
CEEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH
AASTMQAAGGMDWLVHHAGNLMRRHPKYINVLAPMITWCMTIMAGTGYTVFSTLPVIAEV
HHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
AKEIGERPSRPLSAAVVASQVAISGSPISAAMAAMISIMEPAGVSFLQIMAICLPASFIA
HHHHCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
AMVAAFVASRQGCELKDDVIYQQIQKNNKQQTTKKSEAIDPKAKYSVIFFALATCAIVIY
HHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHH
AAIPSLRPAFPSGNIMSTRDIIIVSMFAAACLMVPMCNIHPDRIIRTTTFRSGMSSVAVI
HHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHEEEHHHHHHHHHHHHHH
LGIVTMGSTVVNAHLPEIKEFAGSLLHSYPMLLSLVLFGTCTLLMSQGATTPLIVPLALS
HHHHHHCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEHEEE
LNVPHWAILASFVAVTGVFVLPTYPTSLAAIEFDSTGSTHMGKNIFDHPFMLPGLTGVVV
CCCCHHHHHHHHHHHHHHHEECCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHH
AIGFGFLLAPIVL
HHHHHHHHHHHCC
>Mature Secondary Structure 
SIIIEISVVLICIYWGLKMGGLGVGLAGGLGLAILTLGLGLEPSAPAIDVILIIMSVVT
EEEEHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHH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HHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11004174; 14500908; 8898907 [H]