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The map label for this gene is nrfA

Identifier: 209695454

GI number: 209695454

Start: 2138553

End: 2139989

Strand: Reverse

Name: nrfA

Synonym: VSAL_I1997

Alternate gene names: 209695454

Gene position: 2139989-2138553 (Counterclockwise)

Preceding gene: 209695461

Following gene: 209695450

Centisome position: 64.36

GC content: 44.54

Gene sequence:

>1437_bases
GTGAAAAAGCAATGGACCCGTCGTTCAGCCGCTGCAATTGCCATGGTGACTACGTTATTACTAAGTAGCCACAGCTTTGC
CGCTTCTGAAAAAACAGAGCGAGTTGACCCTCGCAACGAAGCATATGCAAAACAACACGCCGATCAATATGAATCTTGGC
GTCCCACATCAGACAGCGTACAAATTGAAGATGCTTTAGCAGAAGATCCAAACATGGTCATTTTATGGGCCGGTTATGGG
TTTGCTAAAGACTATAATAAAGCCCGTGGCCATTTCTATGCCATTGACGATGTACGTGAAACTTTACGTACTGCAGGCCC
CACCGATGAAAACTCAGGTCCCATGCCGATGGCATGTTGGAGTTGTAAAAGCCCCGACGTTGGTCGTGTTATTGAAGAGC
AAGGCGAAGATGGCTATTTCTCAGGTAAATGGGCACGTCTAGGCAGTGAAATTCAAAACCCAATTGGGTGTGCCGATTGT
CATGACACTCGCAGTGAAGAGTTCAAAAATGGTGGCCCTGCTTTAGCCATCACAAAACCTCATGTTGAACGTGCAATGAA
TGCGATTGGCAAGCCTTTCGATGACCAATCTCGTTTAGATAAACAAGCCTCTGTTTGCGGTCAATGTCATGTTGAATACT
ATTTCACAGGTAAGACTAAAGCCGTTAAGTTCCCTTGGGATAAAGGGACAACCGTTGATGAAATGGAAGAATACTACGAC
GAAATTGGGTTTAGTGATTGGACACACAAAGTATCTAAAGCCCCTATGCTAAAAGCACAACATCCAGGCTATGAAACATG
GCGAGAAGGCATTCACGGTAAAAACAAAGTGGTTTGTGTTGATTGTCATATGCCAAAAGTGACAAGAGAAGACGGTACGG
TTTACACCGATCATAAAGTGGGCAACCCATTCGATCGTTTTGAAGATACTTGTGCAAATTGTCACACGCAATCAAAAGAC
ATGCTTCGTGAAATCGTTTCTAGTCGTAAAGCTCAAGTTTTAAAAATGAAACTAACCGCTGAACGCCAAATTGTCGCGGC
ACATTTTGAGGCAGGTGCAGCATGGGATGCAGGCGCAACTAAAGAAGAAATGGCACCTATCCTACAAGATATTCGCCACG
CACAATGGCGTTGGGATTATGCAATCGCTTCACATGGCATACACATGCATGCACCTGAAGTAGCATTAGAAGTATTAGGT
TCGGCGGTTGATCGTGCCGCAGATGCTCGAACTAAATTGGTTCGACTGCTTGCGAAAAAAGGCATTACTGATCCAATCGA
GATTCCTGATATTTCAACCAAAGCTGCTGCTCAAAAAGCACTAGGTATGGATATGGAAGGCATGGAAGCGGAGAAAAAAC
ACTTCTTAGAAACCGTTGTTCCTAAATGGGATCAACAAGCAAAAGAACGTGAAGCCGCAGATTACCACCCCCTATAA

Upstream 100 bases:

>100_bases
TTCCTTATCTACGATATACTTCTTCTGTTTCCAATTCTGATTGATAGTCGCTCTCACAACGCTTGGCTAATTCATAATAA
GAATATTGGAGATACCAACA

Downstream 100 bases:

>100_bases
ATAAGGCGAAATAACTCACGCTTTATGCAATAACAAAAAAGCCGACAATTCATAAGAATGTCGGCTTTTTATTTGTCTCT
TTTCTTGTTAAATACGACGA

Product: cytochrome c552

Products: NA

Alternate protein names: Ammonia-forming cytochrome c nitrite reductase; Cytochrome c nitrite reductase

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MKKQWTRRSAAAIAMVTTLLLSSHSFAASEKTERVDPRNEAYAKQHADQYESWRPTSDSVQIEDALAEDPNMVILWAGYG
FAKDYNKARGHFYAIDDVRETLRTAGPTDENSGPMPMACWSCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADC
HDTRSEEFKNGGPALAITKPHVERAMNAIGKPFDDQSRLDKQASVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
EIGFSDWTHKVSKAPMLKAQHPGYETWREGIHGKNKVVCVDCHMPKVTREDGTVYTDHKVGNPFDRFEDTCANCHTQSKD
MLREIVSSRKAQVLKMKLTAERQIVAAHFEAGAAWDAGATKEEMAPILQDIRHAQWRWDYAIASHGIHMHAPEVALEVLG
SAVDRAADARTKLVRLLAKKGITDPIEIPDISTKAAAQKALGMDMEGMEAEKKHFLETVVPKWDQQAKEREAADYHPL

Sequences:

>Translated_478_residues
MKKQWTRRSAAAIAMVTTLLLSSHSFAASEKTERVDPRNEAYAKQHADQYESWRPTSDSVQIEDALAEDPNMVILWAGYG
FAKDYNKARGHFYAIDDVRETLRTAGPTDENSGPMPMACWSCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADC
HDTRSEEFKNGGPALAITKPHVERAMNAIGKPFDDQSRLDKQASVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
EIGFSDWTHKVSKAPMLKAQHPGYETWREGIHGKNKVVCVDCHMPKVTREDGTVYTDHKVGNPFDRFEDTCANCHTQSKD
MLREIVSSRKAQVLKMKLTAERQIVAAHFEAGAAWDAGATKEEMAPILQDIRHAQWRWDYAIASHGIHMHAPEVALEVLG
SAVDRAADARTKLVRLLAKKGITDPIEIPDISTKAAAQKALGMDMEGMEAEKKHFLETVVPKWDQQAKEREAADYHPL
>Mature_478_residues
MKKQWTRRSAAAIAMVTTLLLSSHSFAASEKTERVDPRNEAYAKQHADQYESWRPTSDSVQIEDALAEDPNMVILWAGYG
FAKDYNKARGHFYAIDDVRETLRTAGPTDENSGPMPMACWSCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADC
HDTRSEEFKNGGPALAITKPHVERAMNAIGKPFDDQSRLDKQASVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
EIGFSDWTHKVSKAPMLKAQHPGYETWREGIHGKNKVVCVDCHMPKVTREDGTVYTDHKVGNPFDRFEDTCANCHTQSKD
MLREIVSSRKAQVLKMKLTAERQIVAAHFEAGAAWDAGATKEEMAPILQDIRHAQWRWDYAIASHGIHMHAPEVALEVLG
SAVDRAADARTKLVRLLAKKGITDPIEIPDISTKAAAQKALGMDMEGMEAEKKHFLETVVPKWDQQAKEREAADYHPL

Specific function: Plays a role in nitrite reduction

COG id: COG3303

COG function: function code P; Formate-dependent nitrite reductase, periplasmic cytochrome c552 subunit

Gene ontology:

Cell location: Periplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the cytochrome c-552 family

Homologues:

Organism=Escherichia coli, GI1790506, Length=443, Percent_Identity=63.431151241535, Blast_Score=621, Evalue=1e-179,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NRFA_ALISL (B6EH98)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263383.1
- GeneID:   6987503
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1997
- HOGENOM:   HBG488281
- OMA:   PWDMGTT
- ProtClustDB:   PRK11125
- GO:   GO:0006810
- HAMAP:   MF_01182
- InterPro:   IPR003321
- InterPro:   IPR017570
- InterPro:   IPR011031
- PIRSF:   PIRSF000243
- TIGRFAMs:   TIGR03152

Pfam domain/function: PF02335 Cytochrom_C552

EC number: =1.7.2.2

Molecular weight: Translated: 53621; Mature: 53621

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS51008 MULTIHEME_CYTC

Important sites: BINDING 119-119 BINDING 122-122 BINDING 157-157 BINDING 160-160 BINDING 206-206 BINDING 209-209 BINDING 213-213 BINDING 261-261 BINDING 279-279 BINDING 282-282 BINDING 311-311 BINDING 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKQWTRRSAAAIAMVTTLLLSSHSFAASEKTERVDPRNEAYAKQHADQYESWRPTSDSV
CCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCE
QIEDALAEDPNMVILWAGYGFAKDYNKARGHFYAIDDVRETLRTAGPTDENSGPMPMACW
EEHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEE
SCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADCHDTRSEEFKNGGPALAITKP
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEECH
HVERAMNAIGKPFDDQSRLDKQASVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
HHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHH
EIGFSDWTHKVSKAPMLKAQHPGYETWREGIHGKNKVVCVDCHMPKVTREDGTVYTDHKV
HCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEECCCC
GNPFDRFEDTCANCHTQSKDMLREIVSSRKAQVLKMKLTAERQIVAAHFEAGAAWDAGAT
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KEEMAPILQDIRHAQWRWDYAIASHGIHMHAPEVALEVLGSAVDRAADARTKLVRLLAKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITDPIEIPDISTKAAAQKALGMDMEGMEAEKKHFLETVVPKWDQQAKEREAADYHPL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MKKQWTRRSAAAIAMVTTLLLSSHSFAASEKTERVDPRNEAYAKQHADQYESWRPTSDSV
CCCCHHHHHHHHHHHHHHHHHHCCCHHHCCHHHCCCCCHHHHHHHHHHHHHCCCCCCCCE
QIEDALAEDPNMVILWAGYGFAKDYNKARGHFYAIDDVRETLRTAGPTDENSGPMPMACW
EEHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEHHHHHHHHHHCCCCCCCCCCCCEEEE
SCKSPDVGRVIEEQGEDGYFSGKWARLGSEIQNPIGCADCHDTRSEEFKNGGPALAITKP
CCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHCCCCCEEEEECH
HVERAMNAIGKPFDDQSRLDKQASVCGQCHVEYYFTGKTKAVKFPWDKGTTVDEMEEYYD
HHHHHHHHHCCCCCCHHHHHHHHHHHCEEEEEEEEECCCEEEECCCCCCCCHHHHHHHHH
EIGFSDWTHKVSKAPMLKAQHPGYETWREGIHGKNKVVCVDCHMPKVTREDGTVYTDHKV
HCCCCHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEECCCC
GNPFDRFEDTCANCHTQSKDMLREIVSSRKAQVLKMKLTAERQIVAAHFEAGAAWDAGAT
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
KEEMAPILQDIRHAQWRWDYAIASHGIHMHAPEVALEVLGSAVDRAADARTKLVRLLAKK
HHHHHHHHHHHHHHHHHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GITDPIEIPDISTKAAAQKALGMDMEGMEAEKKHFLETVVPKWDQQAKEREAADYHPL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA