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The map label for this gene is rnfE

Identifier: 209695334

GI number: 209695334

Start: 2001278

End: 2001964

Strand: Reverse

Name: rnfE

Synonym: VSAL_I1867

Alternate gene names: 209695334

Gene position: 2001964-2001278 (Counterclockwise)

Preceding gene: 209695335

Following gene: 209695333

Centisome position: 60.21

GC content: 40.17

Gene sequence:

>687_bases
ATGGCAACTCATAAAGAATTAATCAAAAATGGGTTATGGGATAACAACCCCGCTTTAGTCCAATTACTTGGTCTATGCCC
ATTATTAGCAGTCTCTGCAACAGTAACCAATGCATTAGGGTTAGGAATTGCAACAACGTTAGTTCTGATTGGATCAAACT
TAATCGTTTCTTTAGTCCGACAATGGATCCCACAAGAAGTCCGTATTCCTGTGTTTGTTATGATCATTGCCTCTTTAGTT
ACCTGTGTTCAATTATTAATGAATGCTTATGCTTATGGCTTATATCTCTCATTAGGTATTTTCATTCCATTAATCGTTAC
CAACTGTATTATCATTGGTCGAGCCGAAGCCTTTGCTTCTAAAAATGATCCATTACCTGCCGCTCTCGATGGGCTATGGA
TGGGACTAGGCATGACGTCCGTACTTGTTTTATTAGGTGCAATACGTGAGATTCTTGGTAATGGCACTTTATTTGACGGT
GCCGATTTACTTCTTGGCGATTGGGCTACCACCTTGCGAATTGAGCTATTTCATGTTGATAGCCATTTCTTACTTGCAAT
GCTTCCCCCTGGCGCTTTTCTTGGTGTGGGATTTTTAATTGCGATCAAGAATATCATTGATAAGAAACTGGCCGATCGTC
AACCAAAACAAGAAAAAACGGAAATTGAACGCGCTCGCGTCTCTTAA

Upstream 100 bases:

>100_bases
CACCGCGAGCCGTAGTGAAAGCCGTGAAAAGAGCGGTTTGGTTCTATAAAACACATCAAGACGAGCTACAAACTCTCCCA
CTTAATTGCGAGGTGAAATA

Downstream 100 bases:

>100_bases
AGGGAATTGATACTACGATGAACAATGTCAAACGACTCGAAATTTTAACGCGTTTACGTGCAGAGAACCCAAAGCCAGAG
ACTGAACTCGAATGGTCCAG

Product: electron transport complex RsxE subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 227

Protein sequence:

>228_residues
MATHKELIKNGLWDNNPALVQLLGLCPLLAVSATVTNALGLGIATTLVLIGSNLIVSLVRQWIPQEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDPLPAALDGLWMGLGMTSVLVLLGAIREILGNGTLFDG
ADLLLGDWATTLRIELFHVDSHFLLAMLPPGAFLGVGFLIAIKNIIDKKLADRQPKQEKTEIERARVS

Sequences:

>Translated_228_residues
MATHKELIKNGLWDNNPALVQLLGLCPLLAVSATVTNALGLGIATTLVLIGSNLIVSLVRQWIPQEVRIPVFVMIIASLV
TCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDPLPAALDGLWMGLGMTSVLVLLGAIREILGNGTLFDG
ADLLLGDWATTLRIELFHVDSHFLLAMLPPGAFLGVGFLIAIKNIIDKKLADRQPKQEKTEIERARVS
>Mature_227_residues
ATHKELIKNGLWDNNPALVQLLGLCPLLAVSATVTNALGLGIATTLVLIGSNLIVSLVRQWIPQEVRIPVFVMIIASLVT
CVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFASKNDPLPAALDGLWMGLGMTSVLVLLGAIREILGNGTLFDGA
DLLLGDWATTLRIELFHVDSHFLLAMLPPGAFLGVGFLIAIKNIIDKKLADRQPKQEKTEIERARVS

Specific function: May be part of a membrane complex involved in electron transport

COG id: COG4660

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfE

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nqrDE/rnfAE family

Homologues:

Organism=Escherichia coli, GI1787919, Length=210, Percent_Identity=65.2380952380952, Blast_Score=267, Evalue=4e-73,
Organism=Escherichia coli, GI1787914, Length=142, Percent_Identity=37.3239436619718, Blast_Score=81, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNFE_ALISL (B6EGH2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263263.1
- GeneID:   6988920
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1867
- HOGENOM:   HBG624915
- OMA:   VTNCVII
- ProtClustDB:   PRK12405
- GO:   GO:0006810
- HAMAP:   MF_00478
- InterPro:   IPR003667
- InterPro:   IPR010968
- PIRSF:   PIRSF006102
- TIGRFAMs:   TIGR01948

Pfam domain/function: PF02508 Rnf-Nqr

EC number: NA

Molecular weight: Translated: 24622; Mature: 24491

Theoretical pI: Translated: 6.77; Mature: 6.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x20267104)-; HASH(0x21347fac)-; HASH(0x1d7efdf0)-; HASH(0x20c4c3b4)-; HASH(0x2134f980)-; HASH(0x20594fbc)-;

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATHKELIKNGLWDNNPALVQLLGLCPLLAVSATVTNALGLGIATTLVLIGSNLIVSLVR
CCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWIPQEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFAS
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
KNDPLPAALDGLWMGLGMTSVLVLLGAIREILGNGTLFDGADLLLGDWATTLRIELFHVD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCHHHEEEEEEEEEC
SHFLLAMLPPGAFLGVGFLIAIKNIIDKKLADRQPKQEKTEIERARVS
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCH
>Mature Secondary Structure 
ATHKELIKNGLWDNNPALVQLLGLCPLLAVSATVTNALGLGIATTLVLIGSNLIVSLVR
CCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QWIPQEVRIPVFVMIIASLVTCVQLLMNAYAYGLYLSLGIFIPLIVTNCIIIGRAEAFAS
HHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCC
KNDPLPAALDGLWMGLGMTSVLVLLGAIREILGNGTLFDGADLLLGDWATTLRIELFHVD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCHHHHHCHHHEEEEEEEEEC
SHFLLAMLPPGAFLGVGFLIAIKNIIDKKLADRQPKQEKTEIERARVS
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA