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The map label for this gene is pheS

Identifier: 209695218

GI number: 209695218

Start: 1846340

End: 1847323

Strand: Reverse

Name: pheS

Synonym: VSAL_I1733

Alternate gene names: 209695218

Gene position: 1847323-1846340 (Counterclockwise)

Preceding gene: 209695221

Following gene: 209695217

Centisome position: 55.56

GC content: 44.21

Gene sequence:

>984_bases
ATGCAACATCTAGACGAGATCATTGCCAATGCAACGGCAGAAATTGAACAAGCAGGCTCTTTAGTCGCTCTGGACGAAGT
CCGTGTTCAATATTTAGGTAAAAAAGGGCATTTGACACTTCAGCTTCAAGGTCTAGGAAAACTAGATCCGACAGAACGTC
GTGAAGCGGGTCAATTGATCAATAAAGCGAAGCAGACTGTTCAAACGTTATTAGGTGAGCGTAAAGACTCTCTTCAAACT
GCAGAGCTTGAAGCGAAACTTGCTGCTGAAACTATCGATGTAAGCCTTCCGGGCCGTCGTATCGAGAACGGTGGTTTACA
CCCAGTTACTCGTACCGTTGAGCGTATTGAGCAATTCTTTGGTGAGTTAGGCTTTAGTACTGAGTCTGGTCCTGAGATCG
AAGATGCATTCCATAACTTCGATGCATTGAACATTGCTGATGATCACCCTGCGCGTACTGATCACGATACGTTCTTCTTT
AACCCTGATTTAATGCTGCGTACGCACACTTCAGGTGTTCAGATCCGTACAATGGAAAATGGCAAACCCCCATTCCGTTT
CATCGCTCCGGGTCGTGTTTACCGTAACGATTACGATCAAACTCACACACCAATGTTCCACCAAGTGGAAGGTATGTTAG
TTGATGAGAACGTAAACTTTGCACAACTTAAAGGTATTCTTCACGATTTCCTTTGTAATTTCTTTGAAGAAGAAGTTGAA
GTGCGTTTCCGTCCTTCATTTTTCCCATTCACAGAACCTTCTGCTGAAGTTGATGTGAAACGTAAAGATGGCAAGTGGTT
AGAAGTATTAGGCTGTGGCATGGTTCACCCTAACGTACTTCGCTCTGTTGGCATCGACCCTGAAAAATACTCAGGCTTTG
CGTTTGGTATGGGTGTTGAGCGTCTAACTATGCTTCGTTACGGCGTAAATGACCTTCGTGCGTTCTTCGAGAACGACCTT
CGTTTCCTTAAACAATTCAAGTAA

Upstream 100 bases:

>100_bases
ACTTCAATTCAATTTGGATTTAGCGCTCTGATTCATTAGTATGTACAGTAATATGTATTTTACTCGCAACGGATACCACT
AGTAAGTGGATGAGGAAACG

Downstream 100 bases:

>100_bases
TTCGGGGCTAGAAATTTATGAAATTCAGTGAATCTTGGCTACGCGAGTGGGTTAAACCTGCTATTAACAGCGAAGAGCTA
GCTCACCAAATCACAATGGC

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS

Number of amino acids: Translated: 327; Mature: 327

Protein sequence:

>327_residues
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPTERREAGQLINKAKQTVQTLLGERKDSLQT
AELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Sequences:

>Translated_327_residues
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPTERREAGQLINKAKQTVQTLLGERKDSLQT
AELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK
>Mature_327_residues
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPTERREAGQLINKAKQTVQTLLGERKDSLQT
AELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFFGELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFF
NPDLMLRTHTSGVQIRTMENGKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVERLTMLRYGVNDLRAFFENDL
RFLKQFK

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily

Homologues:

Organism=Homo sapiens, GI5729820, Length=345, Percent_Identity=31.0144927536232, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI4758340, Length=259, Percent_Identity=32.8185328185328, Blast_Score=133, Evalue=2e-31,
Organism=Escherichia coli, GI1788007, Length=327, Percent_Identity=73.0886850152905, Blast_Score=509, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI32566635, Length=283, Percent_Identity=33.5689045936396, Blast_Score=137, Evalue=7e-33,
Organism=Caenorhabditis elegans, GI17508957, Length=260, Percent_Identity=31.9230769230769, Blast_Score=130, Evalue=7e-31,
Organism=Caenorhabditis elegans, GI32563657, Length=260, Percent_Identity=31.9230769230769, Blast_Score=130, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6321087, Length=268, Percent_Identity=32.089552238806, Blast_Score=130, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6325304, Length=271, Percent_Identity=27.3062730627306, Blast_Score=103, Evalue=3e-23,
Organism=Drosophila melanogaster, GI18858079, Length=258, Percent_Identity=31.7829457364341, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17137424, Length=279, Percent_Identity=29.0322580645161, Blast_Score=115, Evalue=4e-26,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYFA_ALISL (B6EN08)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002263147.1
- GeneID:   6988971
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I1733
- HOGENOM:   HBG284353
- OMA:   FRASYFP
- ProtClustDB:   PRK00488
- GO:   GO:0005737
- HAMAP:   MF_00281
- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978
- PANTHER:   PTHR11538
- TIGRFAMs:   TIGR00468

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d; SSF46589 tRNA_binding_arm

EC number: =6.1.1.20

Molecular weight: Translated: 37173; Mature: 37173

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPTERREAGQLI
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHH
NKAKQTVQTLLGERKDSLQTAELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFF
HHHHHHHHHHHCCHHCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFFNPDLMLRTHTSGVQIRTMEN
HHHCCCCCCCCCHHHHHCCCCEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEECC
GKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
CCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQHLDEIIANATAEIEQAGSLVALDEVRVQYLGKKGHLTLQLQGLGKLDPTERREAGQLI
CCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHH
NKAKQTVQTLLGERKDSLQTAELEAKLAAETIDVSLPGRRIENGGLHPVTRTVERIEQFF
HHHHHHHHHHHCCHHCCHHHHHHHHHHHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHH
GELGFSTESGPEIEDAFHNFDALNIADDHPARTDHDTFFFNPDLMLRTHTSGVQIRTMEN
HHHCCCCCCCCCHHHHHCCCCEECCCCCCCCCCCCCEEEECCCEEEEEECCCEEEEEECC
GKPPFRFIAPGRVYRNDYDQTHTPMFHQVEGMLVDENVNFAQLKGILHDFLCNFFEEEVE
CCCCEEEECCCHHHCCCCCCCCCCHHHHHCCEEEECCCCHHHHHHHHHHHHHHHHHHHHH
VRFRPSFFPFTEPSAEVDVKRKDGKWLEVLGCGMVHPNVLRSVGIDPEKYSGFAFGMGVE
EEECCCCCCCCCCCCCEEEECCCCCEEEEEECCCCCHHHHHHCCCCHHHCCCEEECCCHH
RLTMLRYGVNDLRAFFENDLRFLKQFK
HHHHHHHCHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA