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The map label for this gene is pheT [H]

Identifier: 209695217

GI number: 209695217

Start: 1843935

End: 1846322

Strand: Reverse

Name: pheT [H]

Synonym: VSAL_I1732

Alternate gene names: 209695217

Gene position: 1846322-1843935 (Counterclockwise)

Preceding gene: 209695218

Following gene: 209695216

Centisome position: 55.53

GC content: 45.14

Gene sequence:

>2388_bases
ATGAAATTCAGTGAATCTTGGCTACGCGAGTGGGTTAAACCTGCTATTAACAGCGAAGAGCTAGCTCACCAAATCACAAT
GGCTGGTTTGGAAGTTGATGAAGTTGCACCTGTTGCTGGTGAGTTCACCGGCGTTAAAGTAGGTAAAGTGGTTGAGTGTG
GTCAACACCCAGACGCAGACAAACTTCGTGTAACTAAAATTGATATCGGCGAAGAAGAGCTTTTAGACATCGTTTGTGGT
GCCTCTAACTGTCGTCTTGGCCTAACAGTAGCAGTAGCAACGGTTGGCGCAGTCCTTCCGGGTGACTTCAAAATTAAGAA
AGCAAAACTACGTGGTGTTCCATCACACGGCATGCTTTGTTCTTTCTCTGAGCTAGGTATTGATGTTGAATCTGACGGTA
TTTTAGAATTACCAGAAGGCTCTACATTAGGTATGGACGTTCGTGAGCTTCTTGAGCTTAACGATGTTGCTATCGATGTT
GACCTAACAGCAAACCGTGCGGACTGTTTCAGCATTCGTGGTTTAGCTCGTGAAGTCGGTGTTCTTAACCGTGCAGACGT
AACAGAACCAACTATTGAAGCGGTAGCTACAAGCATTGAAGATACCGTTTCTATTGACATTAAAGCAACGGAGGCGTGTC
CACGTTACCTTGGTCGTGTTATCAAGAACGTAAACGCAAAAGCGGAAACGCCAATTTGGATGCAAGAGAAACTGCGTCGT
TGCGGTATTCGTTCAATCGATGCGATTGTTGATATTACAAACTATGTAATGCTTGAGCAAGGCCAACCAATGCACGCGTT
TGATCTGTCTAAGATCGACGGCGGTATTGTGGTTCGTATGGCTGAACAAGATGAGAAATTAACTCTTCTTGATGGCAACG
AAGCAAAACTAAACAGCAATACACTTGTTATCGCTGACCAAAACAAAGCACTAGCAATTGCTGGTATCTTTGGTGGTAAA
GAATCCGGTGTTACGTCTGAAACAACAGATATCATGCTTGAGTGTGCATTCTTCGCACCTGATCACATCCGTGGTCGCGC
TCGTGCATACGGTCTACATACAGATTCATCTCTACGTTTTGAGCGTGGTGTTGATTCTACATTACAAGCAACAGCGATGG
AGCGTGCAACACAGCTTCTTGTTGAACTTTGTGGTGGTGAAGTTGCGCCTATTTCTGCGGCTGAATCTGAAGCCGATCTT
CCAAAAGAAAGCACCGTTGCGTTACGTCGCAGCAAACTAGACGGTCTTTTAGGTCATCATATCCCATCAACAGATGTGGT
TGAGATCCTTACTCGTTTAGGTTGTGTTGTTGAGACAACGGATACAGGTTGGACAGCAACATCTCCATCATGGCGTTTTG
ATATTGCAATCGAGCAAGACCTAATTGAAGAAGTAGGTCGTATCTACGGTTACAATAACATTCCAAACCAAGCCCCAGTT
GCTGCGTTAAACATGAATCTTCATAAAGAAGCAAACCAACCGCTTAAGCGTGTTCGTGATCTTCTTGTGGACCGTGGCTA
CCACGAAGCAATTACCTACAGCTTCGTAGAGCCAGAGCAGCAAAAACTGATCGTGCCTGAGATTGAGCCGTTAATTCTGC
CATTTCCAATTTCAGCAGACATGTCAGCGATGCGTCTAAGCCTATGGACTGGTTTGATCAATACCGTTGTTCATAACCAA
AAACGTCAACAACCACGCGTTCGTTTATTCGAATCTGGTTTACGTTTTATTCCTGAAGAATCAGCAGAAAACGGCATGCG
TCAAGAAATGATGCTTGCTGGTATCATCGCTGGTACTCGTGGTGAAGAGCATTGGGATATCGCAACAAACACCGTTGACT
TTTTCGACCTTAAAGGCGATTTAGAAGCTGTACTTGAACTAACTGCTAACGAATTAGCGTACGAGTTTAAAGCAGCACAA
CACCCAGCACTTCACCCAGGTCAAACAGCGGCTATCGTTGTTGACGGAAAAGAAGTGGGTTTCATCGGTACAGTACACCC
AGAGCTAGAGCGTAAGTTTGGCTTGAACGGTCGTACAATCATGTTTGAAATCGAGTGGGACGCAATCAACACTCGCGTTA
TCCCTGAAGCGGCAGCAGTATCTAAGTTCCCTGCAAACCGTCGTGATATCGCGATTGTTGCTGATGAAGCCATTGCTTCT
GGTGACATCGTTGCTGAGTGTTTAGCATCAGGTGGCGAGTTATTAACTAGCGCGAAATTGTTTGATGTTTACCGCGGTCA
AGGTGTTGAAGATGATAAGAAGAGTCTGGCAATCGCACTGACACTTCAATCTGTTGAGCGTACATTAGAAGAGGCTGATA
TCACTTCAGCTGTTGACGCTATCGTTGCGGCAATTGGTGAGAAGTTCTCAGCAACACTGCGTGACTAA

Upstream 100 bases:

>100_bases
GTCTAACTATGCTTCGTTACGGCGTAAATGACCTTCGTGCGTTCTTCGAGAACGACCTTCGTTTCCTTAAACAATTCAAG
TAATTCGGGGCTAGAAATTT

Downstream 100 bases:

>100_bases
TTAGCTCTTTTAGCTAAATATAAACACAATGAATCCAGTATCAATGAAGGTTGATACTGGATTTTTTTTATAATCGAACC
TATAAATAATAGGGGAGTAT

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 795; Mature: 795

Protein sequence:

>795_residues
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDADKLRVTKIDIGEEELLDIVCG
ASNCRLGLTVAVATVGAVLPGDFKIKKAKLRGVPSHGMLCSFSELGIDVESDGILELPEGSTLGMDVRELLELNDVAIDV
DLTANRADCFSIRGLAREVGVLNRADVTEPTIEAVATSIEDTVSIDIKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CGIRSIDAIVDITNYVMLEQGQPMHAFDLSKIDGGIVVRMAEQDEKLTLLDGNEAKLNSNTLVIADQNKALAIAGIFGGK
ESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRFERGVDSTLQATAMERATQLLVELCGGEVAPISAAESEADL
PKESTVALRRSKLDGLLGHHIPSTDVVEILTRLGCVVETTDTGWTATSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
AALNMNLHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISADMSAMRLSLWTGLINTVVHNQ
KRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTRGEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAQ
HPALHPGQTAAIVVDGKEVGFIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAAAVSKFPANRRDIAIVADEAIAS
GDIVAECLASGGELLTSAKLFDVYRGQGVEDDKKSLAIALTLQSVERTLEEADITSAVDAIVAAIGEKFSATLRD

Sequences:

>Translated_795_residues
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDADKLRVTKIDIGEEELLDIVCG
ASNCRLGLTVAVATVGAVLPGDFKIKKAKLRGVPSHGMLCSFSELGIDVESDGILELPEGSTLGMDVRELLELNDVAIDV
DLTANRADCFSIRGLAREVGVLNRADVTEPTIEAVATSIEDTVSIDIKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CGIRSIDAIVDITNYVMLEQGQPMHAFDLSKIDGGIVVRMAEQDEKLTLLDGNEAKLNSNTLVIADQNKALAIAGIFGGK
ESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRFERGVDSTLQATAMERATQLLVELCGGEVAPISAAESEADL
PKESTVALRRSKLDGLLGHHIPSTDVVEILTRLGCVVETTDTGWTATSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
AALNMNLHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISADMSAMRLSLWTGLINTVVHNQ
KRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTRGEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAQ
HPALHPGQTAAIVVDGKEVGFIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAAAVSKFPANRRDIAIVADEAIAS
GDIVAECLASGGELLTSAKLFDVYRGQGVEDDKKSLAIALTLQSVERTLEEADITSAVDAIVAAIGEKFSATLRD
>Mature_795_residues
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDADKLRVTKIDIGEEELLDIVCG
ASNCRLGLTVAVATVGAVLPGDFKIKKAKLRGVPSHGMLCSFSELGIDVESDGILELPEGSTLGMDVRELLELNDVAIDV
DLTANRADCFSIRGLAREVGVLNRADVTEPTIEAVATSIEDTVSIDIKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CGIRSIDAIVDITNYVMLEQGQPMHAFDLSKIDGGIVVRMAEQDEKLTLLDGNEAKLNSNTLVIADQNKALAIAGIFGGK
ESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRFERGVDSTLQATAMERATQLLVELCGGEVAPISAAESEADL
PKESTVALRRSKLDGLLGHHIPSTDVVEILTRLGCVVETTDTGWTATSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
AALNMNLHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISADMSAMRLSLWTGLINTVVHNQ
KRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTRGEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAQ
HPALHPGQTAAIVVDGKEVGFIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAAAVSKFPANRRDIAIVADEAIAS
GDIVAECLASGGELLTSAKLFDVYRGQGVEDDKKSLAIALTLQSVERTLEEADITSAVDAIVAAIGEKFSATLRD

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI124028525, Length=567, Percent_Identity=22.5749559082892, Blast_Score=78, Evalue=4e-14,
Organism=Escherichia coli, GI1788006, Length=795, Percent_Identity=62.0125786163522, Blast_Score=1022, Evalue=0.0,
Organism=Drosophila melanogaster, GI21355211, Length=549, Percent_Identity=23.4972677595628, Blast_Score=101, Evalue=2e-21,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005146
- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR020825
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03483 B3_4; PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 86607; Mature: 86607

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDAD
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCEEECCCCCCCC
KLRVTKIDIGEEELLDIVCGASNCRLGLTVAVATVGAVLPGDFKIKKAKLRGVPSHGMLC
CEEEEEECCCHHHHHHHHCCCCCCCEEHEEHHHHHHHCCCCCCEEEEHHHCCCCCCCCEE
SFSELGIDVESDGILELPEGSTLGMDVRELLELNDVAIDVDLTANRADCFSIRGLAREVG
EHHHHCCEECCCCEEECCCCCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHC
VLNRADVTEPTIEAVATSIEDTVSIDIKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CCCCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAIVDITNYVMLEQGQPMHAFDLSKIDGGIVVRMAEQDEKLTLLDGNEAKLNSN
CCCCHHHHHHHHHHEEEECCCCCCCEEEHHHCCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQNKALAIAGIFGGKESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRF
EEEEEECCCEEEEEEEECCCCCCCCCCCHHEEEEEEECCCHHHCCCCEEEEECCCCCCCH
ERGVDSTLQATAMERATQLLVELCGGEVAPISAAESEADLPKESTVALRRSKLDGLLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
IPSTDVVEILTRLGCVVETTDTGWTATSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
CCCHHHHHHHHHCCCEEEECCCCCEECCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCE
AALNMNLHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAD
EEEECCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEECCCCCCEEEECCCCCC
MSAMRLSLWTGLINTVVHNQKRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTR
HHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCC
GEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAQHPALHPGQTAAIVVDGKEVG
CCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
FIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAAAVSKFPANRRDIAIVADEAIAS
EEECCCHHHHHHHCCCCCEEEEEEEECCCCCEECCCHHHHHCCCCCCCCEEEEECCCCCC
GDIVAECLASGGELLTSAKLFDVYRGQGVEDDKKSLAIALTLQSVERTLEEADITSAVDA
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
IVAAIGEKFSATLRD
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSESWLREWVKPAINSEELAHQITMAGLEVDEVAPVAGEFTGVKVGKVVECGQHPDAD
CCCCHHHHHHHHHHCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCEEECCEEECCCCCCCC
KLRVTKIDIGEEELLDIVCGASNCRLGLTVAVATVGAVLPGDFKIKKAKLRGVPSHGMLC
CEEEEEECCCHHHHHHHHCCCCCCCEEHEEHHHHHHHCCCCCCEEEEHHHCCCCCCCCEE
SFSELGIDVESDGILELPEGSTLGMDVRELLELNDVAIDVDLTANRADCFSIRGLAREVG
EHHHHCCEECCCCEEECCCCCCCCCCHHHHHHHCCEEEEEEECCCCCHHHHHHHHHHHHC
VLNRADVTEPTIEAVATSIEDTVSIDIKATEACPRYLGRVIKNVNAKAETPIWMQEKLRR
CCCCCCCCCHHHHHHHHCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHH
CGIRSIDAIVDITNYVMLEQGQPMHAFDLSKIDGGIVVRMAEQDEKLTLLDGNEAKLNSN
CCCCHHHHHHHHHHEEEECCCCCCCEEEHHHCCCCEEEEEECCCCEEEEEECCCCEECCC
TLVIADQNKALAIAGIFGGKESGVTSETTDIMLECAFFAPDHIRGRARAYGLHTDSSLRF
EEEEEECCCEEEEEEEECCCCCCCCCCCHHEEEEEEECCCHHHCCCCEEEEECCCCCCCH
ERGVDSTLQATAMERATQLLVELCGGEVAPISAAESEADLPKESTVALRRSKLDGLLGHH
HCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCC
IPSTDVVEILTRLGCVVETTDTGWTATSPSWRFDIAIEQDLIEEVGRIYGYNNIPNQAPV
CCCHHHHHHHHHCCCEEEECCCCCEECCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCE
AALNMNLHKEANQPLKRVRDLLVDRGYHEAITYSFVEPEQQKLIVPEIEPLILPFPISAD
EEEECCHHHHHHHHHHHHHHHHHCCCCHHHEEEEECCCCCCEEECCCCCCEEEECCCCCC
MSAMRLSLWTGLINTVVHNQKRQQPRVRLFESGLRFIPEESAENGMRQEMMLAGIIAGTR
HHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHCCCCHHHHCCHHHHHHHHHHHCCCC
GEEHWDIATNTVDFFDLKGDLEAVLELTANELAYEFKAAQHPALHPGQTAAIVVDGKEVG
CCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCC
FIGTVHPELERKFGLNGRTIMFEIEWDAINTRVIPEAAAVSKFPANRRDIAIVADEAIAS
EEECCCHHHHHHHCCCCCEEEEEEEECCCCCEECCCHHHHHCCCCCCCCEEEEECCCCCC
GDIVAECLASGGELLTSAKLFDVYRGQGVEDDKKSLAIALTLQSVERTLEEADITSAVDA
CHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHH
IVAAIGEKFSATLRD
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA