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The map label for this gene is ppsA [H]

Identifier: 209695211

GI number: 209695211

Start: 1837282

End: 1838166

Strand: Reverse

Name: ppsA [H]

Synonym: VSAL_I1726

Alternate gene names: 209695211

Gene position: 1838166-1837282 (Counterclockwise)

Preceding gene: 209695214

Following gene: 209695209

Centisome position: 55.28

GC content: 38.64

Gene sequence:

>885_bases
ATGACTAATACTATTTTACCAACGTTAACTCGCGTAGCTAGTTTACCGTCTTATCAATCAAGCACCGATTCAAATGCATC
TTTGTATGTGTCTATCGCAGGGCTTATTCAAGAGCATTTGTTTTATCATCCAAAGGCCGTGATTAATACGCCAAAGATGA
GTGACATTGATGCGCAAAGTCTTGCGGCAATAATAGAAAGTGCATCTGATGGTAAAACCCATTTTGTTGATACGTTATCT
ACTGTGATTCTTGACAATATTACTGACGAAACAAAAGAAGTGAAAATTGCTTTTTCTGATGCAGACAGCAGTGCGTTTTC
AGGCTTACTTGTTGGTCAATTAGAACCAAAAGAAATGAACCCAATGATGGGCGTTCGTGGTGTAACACGTTATGCATCGG
ATGAAAGTTCTGAGCAATTTGCATTAGAGTGTGCTGTAATTAAAAAAGTCATTGCTTCAACTTCGGCAGTGGTTCAAATT
GTCGTTCCATTTGTTCGCAACTTAAGTGACGCAGCTACGATTATTGACCGTTTAGCGATTTATGGTTTGCCTCGTGGACT
GAATGGCCTAAAAGTACACTTTGTGTGTGATGTACCTGCTGCATCATTAATGGCTGAGAAATTATTACAATATTTTGATG
GTTTGGTTGTTAATGTTGATACGTTAACGCAGTTTACACTGGGTGTAGATAAACAAAATGAAGCGCTAGAGCATCATTTT
GAACCACAGAATGAAGCCGTATTACATTTGATCAGTTATGCAATTCAAGCGGCGAATAAAGCCAATAAACCCGTAATGAT
AACCATTGATGAATTGGCTCAAAATACGCGAGTTCAAGAGCTATTGGATCAATTGAATGCAACCGATGTGGTTGTTACTG
CGTAA

Upstream 100 bases:

>100_bases
GGCTTGAGTTGTTGTTAATGTATCGGTATGTTCAACATAAATAACATGTAATCATTCATAAATATAATTAAACTGAATCA
ATGAGTTCATTGGAGCAAGC

Downstream 100 bases:

>100_bases
TTAATACGCTTAACCATTGTATTTGTCTCGTTTTAGAACACGTTGACGATTGATAGTAAAAAGCCTCCAACTCAAAAAAC
AATGAGTGGAGGCTTTTTAA

Product: PEP-utilising enzyme

Products: NA

Alternate protein names: PEP synthase; Pyruvate, water dikinase [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MTNTILPTLTRVASLPSYQSSTDSNASLYVSIAGLIQEHLFYHPKAVINTPKMSDIDAQSLAAIIESASDGKTHFVDTLS
TVILDNITDETKEVKIAFSDADSSAFSGLLVGQLEPKEMNPMMGVRGVTRYASDESSEQFALECAVIKKVIASTSAVVQI
VVPFVRNLSDAATIIDRLAIYGLPRGLNGLKVHFVCDVPAASLMAEKLLQYFDGLVVNVDTLTQFTLGVDKQNEALEHHF
EPQNEAVLHLISYAIQAANKANKPVMITIDELAQNTRVQELLDQLNATDVVVTA

Sequences:

>Translated_294_residues
MTNTILPTLTRVASLPSYQSSTDSNASLYVSIAGLIQEHLFYHPKAVINTPKMSDIDAQSLAAIIESASDGKTHFVDTLS
TVILDNITDETKEVKIAFSDADSSAFSGLLVGQLEPKEMNPMMGVRGVTRYASDESSEQFALECAVIKKVIASTSAVVQI
VVPFVRNLSDAATIIDRLAIYGLPRGLNGLKVHFVCDVPAASLMAEKLLQYFDGLVVNVDTLTQFTLGVDKQNEALEHHF
EPQNEAVLHLISYAIQAANKANKPVMITIDELAQNTRVQELLDQLNATDVVVTA
>Mature_293_residues
TNTILPTLTRVASLPSYQSSTDSNASLYVSIAGLIQEHLFYHPKAVINTPKMSDIDAQSLAAIIESASDGKTHFVDTLST
VILDNITDETKEVKIAFSDADSSAFSGLLVGQLEPKEMNPMMGVRGVTRYASDESSEQFALECAVIKKVIASTSAVVQIV
VPFVRNLSDAATIIDRLAIYGLPRGLNGLKVHFVCDVPAASLMAEKLLQYFDGLVVNVDTLTQFTLGVDKQNEALEHHFE
PQNEAVLHLISYAIQAANKANKPVMITIDELAQNTRVQELLDQLNATDVVVTA

Specific function: Catalyzes the phosphorylation of pyruvate to phosphoenolpyruvate [H]

COG id: COG0574

COG function: function code G; Phosphoenolpyruvate synthase/pyruvate phosphate dikinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1787994, Length=178, Percent_Identity=41.0112359550562, Blast_Score=147, Evalue=8e-37,
Organism=Escherichia coli, GI1788756, Length=173, Percent_Identity=27.1676300578035, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR008279
- InterPro:   IPR006319
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR002192
- InterPro:   IPR015813 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF02896 PEP-utilizers_C; PF01326 PPDK_N [H]

EC number: =2.7.9.2 [H]

Molecular weight: Translated: 31879; Mature: 31747

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTILPTLTRVASLPSYQSSTDSNASLYVSIAGLIQEHLFYHPKAVINTPKMSDIDAQS
CCCCHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCHHH
LAAIIESASDGKTHFVDTLSTVILDNITDETKEVKIAFSDADSSAFSGLLVGQLEPKEMN
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHCCEEEECCCCHHCC
PMMGVRGVTRYASDESSEQFALECAVIKKVIASTSAVVQIVVPFVRNLSDAATIIDRLAI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YGLPRGLNGLKVHFVCDVPAASLMAEKLLQYFDGLVVNVDTLTQFTLGVDKQNEALEHHF
HCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCHHHHHHCC
EPQNEAVLHLISYAIQAANKANKPVMITIDELAQNTRVQELLDQLNATDVVVTA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHHHHCCCCCEEEEC
>Mature Secondary Structure 
TNTILPTLTRVASLPSYQSSTDSNASLYVSIAGLIQEHLFYHPKAVINTPKMSDIDAQS
CCCHHHHHHHHHCCCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHHHCCCCCCCCCCHHH
LAAIIESASDGKTHFVDTLSTVILDNITDETKEVKIAFSDADSSAFSGLLVGQLEPKEMN
HHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCHHHCCEEEECCCCHHCC
PMMGVRGVTRYASDESSEQFALECAVIKKVIASTSAVVQIVVPFVRNLSDAATIIDRLAI
CCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
YGLPRGLNGLKVHFVCDVPAASLMAEKLLQYFDGLVVNVDTLTQFTLGVDKQNEALEHHF
HCCCCCCCCEEEEEEECCCHHHHHHHHHHHHHCCEEEEHHHHHHHHCCCCCCHHHHHHCC
EPQNEAVLHLISYAIQAANKANKPVMITIDELAQNTRVQELLDQLNATDVVVTA
CCCHHHHHHHHHHHHHHHCCCCCCEEEEHHHHHCCCHHHHHHHHCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1310524; 9097039; 9278503 [H]