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The map label for this gene is gatA [H]

Identifier: 209695179

GI number: 209695179

Start: 1780153

End: 1781319

Strand: Reverse

Name: gatA [H]

Synonym: VSAL_I1685

Alternate gene names: 209695179

Gene position: 1781319-1780153 (Counterclockwise)

Preceding gene: 209695182

Following gene: 209695172

Centisome position: 53.57

GC content: 40.62

Gene sequence:

>1167_bases
ATGGATGTTCAAACCACTTATGTGCCTCACTCAGGACCTGTACAGCAATGGGCTTTTTATGGACTTTTAGAAAATAAGAA
ATTTGTTGTAAGCGATTGTATTGATATTAAAGATACGCCAAATGGCTTAGGTAATCCGGTATGGTTAGCGCAACAGAAAG
TGGCCAAAGATAACGCGCCAGTGGTGGATCTTTTATTAAAGAAAGGGGCAATATTTGTGGGTAAAACTCAAATGGATGAG
TTTGGTTTTGGACTTCATGGTAATAACCCACATTACGCTGCGATTAAAAACCCAAACAGTGAAGGGCGGTTAATTGGTGG
GTCATCTTGCGGAGCCATTGCGGCGGTCGCGAATGGATTGGCTGATATCGGCTTAGGCACGGATTTAAGTGGTGGAATAC
GAGTCCCTGCGGTATACGCTGGTTTATATGGTTTTAAATCGTCTTCTTATGCCGCGAATATGAAAGGGGTTTATTGCATT
GCAAAAGAAGAAACCTCTTTAGGCTGGGTTGCTAAGAGTTTGTCGGACATTAGAAAAGTCGCGACGACATTAACTCCGAT
GTCTCCATTAATGACGGTCGATCGCATTGTCGTGCTAGACAGCTTGTTTCTTGATGTTCCAGAAGAAGCAAAGGTAAAAC
TAGAACAATTACTTGAGGCATCCCCTTATTATAAAGTCGTTCGTAGTAAAGCGATCAGTAAAATAATAACCATGAAAGCC
GCCGAAAGTTTTAAAGTCATTACCGCGACAAGAGGGCTACGTAATATAGAACCGTGGGTTGAAAAAAACCACCCAATGCT
CAGTAATGAAATTAAAATGCAATTGAAATGGTTATCTTCATTAAAGTATAAAGATGAGCGAATTGCCCTAGAACAAAGGG
ATCTTGTTAAATCGGTTTTAGAGTCACTTTTAGATGAAAAAACACTGTTGCTACTGCCAACGACCGCTAACATTGCCCCA
CCAGAAACGGCGACGGATGAGCAGTTATACAAACTTAACTCATTGATATTAAAATACACATCATTAGCCAGTTTAGCTGA
TTTACCTCAATTGCATCTTCCGTGGTTTACTGTGAATAACTCACCTTGGGGAGTGTCATTAATCGGTAAAAAAGGAATGG
ATAGACAACTAATTGATGCGGCTATTCGATGGAAAGGGAACCAATAA

Upstream 100 bases:

>100_bases
CACACTACTGATTTGTTTGTTGATACAGTGAATCGCTTGGGTTGTATTCACTACCTCTACTGGTATGCTATTTTTTTTAT
ATTAGCAGCAGTACTTCATT

Downstream 100 bases:

>100_bases
ACAGAGATAAAAATAGAGGAGGCTGATGCATCCTCTATTTTTTTAAGTCAAAATCTATAAAGACACGTATTCTAATATCA
TGTTTGGACTCAGATCTTTA

Product: amidase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MDVQTTYVPHSGPVQQWAFYGLLENKKFVVSDCIDIKDTPNGLGNPVWLAQQKVAKDNAPVVDLLLKKGAIFVGKTQMDE
FGFGLHGNNPHYAAIKNPNSEGRLIGGSSCGAIAAVANGLADIGLGTDLSGGIRVPAVYAGLYGFKSSSYAANMKGVYCI
AKEETSLGWVAKSLSDIRKVATTLTPMSPLMTVDRIVVLDSLFLDVPEEAKVKLEQLLEASPYYKVVRSKAISKIITMKA
AESFKVITATRGLRNIEPWVEKNHPMLSNEIKMQLKWLSSLKYKDERIALEQRDLVKSVLESLLDEKTLLLLPTTANIAP
PETATDEQLYKLNSLILKYTSLASLADLPQLHLPWFTVNNSPWGVSLIGKKGMDRQLIDAAIRWKGNQ

Sequences:

>Translated_388_residues
MDVQTTYVPHSGPVQQWAFYGLLENKKFVVSDCIDIKDTPNGLGNPVWLAQQKVAKDNAPVVDLLLKKGAIFVGKTQMDE
FGFGLHGNNPHYAAIKNPNSEGRLIGGSSCGAIAAVANGLADIGLGTDLSGGIRVPAVYAGLYGFKSSSYAANMKGVYCI
AKEETSLGWVAKSLSDIRKVATTLTPMSPLMTVDRIVVLDSLFLDVPEEAKVKLEQLLEASPYYKVVRSKAISKIITMKA
AESFKVITATRGLRNIEPWVEKNHPMLSNEIKMQLKWLSSLKYKDERIALEQRDLVKSVLESLLDEKTLLLLPTTANIAP
PETATDEQLYKLNSLILKYTSLASLADLPQLHLPWFTVNNSPWGVSLIGKKGMDRQLIDAAIRWKGNQ
>Mature_388_residues
MDVQTTYVPHSGPVQQWAFYGLLENKKFVVSDCIDIKDTPNGLGNPVWLAQQKVAKDNAPVVDLLLKKGAIFVGKTQMDE
FGFGLHGNNPHYAAIKNPNSEGRLIGGSSCGAIAAVANGLADIGLGTDLSGGIRVPAVYAGLYGFKSSSYAANMKGVYCI
AKEETSLGWVAKSLSDIRKVATTLTPMSPLMTVDRIVVLDSLFLDVPEEAKVKLEQLLEASPYYKVVRSKAISKIITMKA
AESFKVITATRGLRNIEPWVEKNHPMLSNEIKMQLKWLSSLKYKDERIALEQRDLVKSVLESLLDEKTLLLLPTTANIAP
PETATDEQLYKLNSLILKYTSLASLADLPQLHLPWFTVNNSPWGVSLIGKKGMDRQLIDAAIRWKGNQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=179, Percent_Identity=30.1675977653631, Blast_Score=71, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17543272, Length=123, Percent_Identity=34.1463414634146, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=388, Percent_Identity=26.8041237113402, Blast_Score=72, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323950, Length=158, Percent_Identity=36.7088607594937, Blast_Score=71, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356731, Length=159, Percent_Identity=32.0754716981132, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI45550774, Length=194, Percent_Identity=28.3505154639175, Blast_Score=69, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24648435, Length=194, Percent_Identity=28.3505154639175, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648437, Length=194, Percent_Identity=28.3505154639175, Blast_Score=68, Evalue=9e-12,
Organism=Drosophila melanogaster, GI24648441, Length=186, Percent_Identity=26.8817204301075, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24648439, Length=186, Percent_Identity=26.8817204301075, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: NA

Molecular weight: Translated: 42587; Mature: 42587

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDVQTTYVPHSGPVQQWAFYGLLENKKFVVSDCIDIKDTPNGLGNPVWLAQQKVAKDNAP
CCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEHHHHHHHCCCCC
VVDLLLKKGAIFVGKTQMDEFGFGLHGNNPHYAAIKNPNSEGRLIGGSSCGAIAAVANGL
HHHHHHHCCCEEEECCCHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHH
ADIGLGTDLSGGIRVPAVYAGLYGFKSSSYAANMKGVYCIAKEETSLGWVAKSLSDIRKV
HHCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
ATTLTPMSPLMTVDRIVVLDSLFLDVPEEAKVKLEQLLEASPYYKVVRSKAISKIITMKA
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AESFKVITATRGLRNIEPWVEKNHPMLSNEIKMQLKWLSSLKYKDERIALEQRDLVKSVL
CCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ESLLDEKTLLLLPTTANIAPPETATDEQLYKLNSLILKYTSLASLADLPQLHLPWFTVNN
HHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
SPWGVSLIGKKGMDRQLIDAAIRWKGNQ
CCCCEEEECCCCCCHHHHHHHHHCCCCC
>Mature Secondary Structure
MDVQTTYVPHSGPVQQWAFYGLLENKKFVVSDCIDIKDTPNGLGNPVWLAQQKVAKDNAP
CCCCEEECCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCCCCEEHHHHHHHCCCCC
VVDLLLKKGAIFVGKTQMDEFGFGLHGNNPHYAAIKNPNSEGRLIGGSSCGAIAAVANGL
HHHHHHHCCCEEEECCCHHHCCCCCCCCCCCEEEEECCCCCCCEEECCCCHHHHHHHHHH
ADIGLGTDLSGGIRVPAVYAGLYGFKSSSYAANMKGVYCIAKEETSLGWVAKSLSDIRKV
HHCCCCCCCCCCEECHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHH
ATTLTPMSPLMTVDRIVVLDSLFLDVPEEAKVKLEQLLEASPYYKVVRSKAISKIITMKA
HHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
AESFKVITATRGLRNIEPWVEKNHPMLSNEIKMQLKWLSSLKYKDERIALEQRDLVKSVL
CCCEEEEEECCCCCCCCHHHHCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
ESLLDEKTLLLLPTTANIAPPETATDEQLYKLNSLILKYTSLASLADLPQLHLPWFTVNN
HHHHCCCEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECC
SPWGVSLIGKKGMDRQLIDAAIRWKGNQ
CCCCEEEECCCCCCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]