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The map label for this gene is opuD [H]

Identifier: 209694796

GI number: 209694796

Start: 1362933

End: 1364456

Strand: Direct

Name: opuD [H]

Synonym: VSAL_I1254

Alternate gene names: 209694796

Gene position: 1362933-1364456 (Clockwise)

Preceding gene: 209694795

Following gene: 209694797

Centisome position: 40.99

GC content: 40.22

Gene sequence:

>1524_bases
ATGTTAAAATCAATACGCCCTTTGGTGTTTTTCCCTACATTTTTAGTTTTAGTGGCAGCACTGATTCTCAGTCTTGTGGA
TCTAAAAAGTTTTACTGCGTTAACAAGCGCCGCAAATAGCTTTGTATTGGATAAGTTCTCTTGGTTATTTAGTTTCGGTA
GTTTTTATCTTTTAGTTCTCATCGTCATTACCTATTTTTCTAAAATCAGTGACGTCCGTATTGGCGGAGAAAACAGTGTT
CCACGCATCAGTAAGCCACGGTGGTTTATGATTGTGTTATGTACCACATTAGCTGTTGGTGTTCTTTTCTGGACCACTGC
GGAACCTATCTATCATTTACATACACCACCAGAAAGTCTGGGTATAGAGCCGGGCAGTCCGAATGCAATCTTGTTTGCTT
TTTCTGCTATGTTTTTACATTGGGGACCAACGCCTTACGCTATTTATGCGGTTCCGGCTCTCATCTTCGCACTGACTTTT
TATAATTTAAAATTACCATTCTCTATTACTAGCCCATTACGTCCAATCTTTGGTCGTTATATTAATGATAAAGTTGCGGA
TGTGATTGATGCGCTTGCTTTATATTCACTGGTTGTGGGCATGGCATCGTCATTAGGTACGGGTGCGTTAACGATGCTTG
GCGGTGTCAGTCAGTTTATAGATATTGAGAGAAACGCCGTTACTTTAGGCATATTCATTGCCATGATTGTTGTAACCTTT
TTAGTGTCTGCTGCGAGTGGGTTAACAAAGGGGATTGCTCGACTTTCTGCGGCCAATTTTTGGATGCTTATTGCATTACT
TGTGTTTGTTTTTATCTGTGGACCGACCGTATATATCTTATCTATCGGGGTTGAAGGGCTAGGTGCTTATTTACAAAACT
TCTTCCGATTAAGTTTATTTACCGGACAGGCTGCGAATGACCCTTGGCCTCAATATTGGAGTGTGTTCTATTGGGCGGTT
TGGTTTGCTTGGGCGCCTATTACCGCAATGTTCTTGGGGAAAATTGCACGAGGCTATACCGTAAAAGAGTTTATCCAAAT
GAATGTTATTTATCCTAGTTTATTTATTCTTGTTTGGGTTTCGGTTTTTTCTGGAACCGCTGTTCACATGGATACGGAAA
CGAACGGTGGATTGAATGATATATTAAGTAATGGTGGTATTGAGCAATTATTGTATTACATCGTACATCAATTACCATTT
GGTGGAGTAACATCGTTTTTCTTAGTCTTAGTTGCTTTTATTTCTTACGTAACCGCGGCCGATTCGAGTACGGATGCGAT
TGGTGATCTTTGTACTAAAGGGTTCAACTCCGATTCAGAAGACGGTACGTCATTACCGATTAAGATGCTCTGGGGAACGA
TCATTGGTTTGGTTGCTTGGATAATGGTGAGTACGGTTGGTATCGATGGGGTTAAACAGTTGTCTAATTTAGGCGGATTA
CCTGCAACTATTATTATTCTCTGTTGTAGCTTAACTCTGATTTATTGGATACGAAAACCAGAGATGCTTACCGGAAAGAA
ATAA

Upstream 100 bases:

>100_bases
CAAACTGGAAAGCTTTTTATGACAATGATGTCGTTTTTGAAAAGCAGTTTACAGAAACTATCAAGCGTCACTTTATGTAA
GCACGTTCAAGGAAGAAATC

Downstream 100 bases:

>100_bases
TCGTGAGTACAGCGTAAAATAAAAAGATCGCAGCCATTAATAAGCTGCGATTTTTTTATAATGGGATTACAGTGAGAAAG
ATATTACGTTTACGTCATTA

Product: transporter BCCT family

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MLKSIRPLVFFPTFLVLVAALILSLVDLKSFTALTSAANSFVLDKFSWLFSFGSFYLLVLIVITYFSKISDVRIGGENSV
PRISKPRWFMIVLCTTLAVGVLFWTTAEPIYHLHTPPESLGIEPGSPNAILFAFSAMFLHWGPTPYAIYAVPALIFALTF
YNLKLPFSITSPLRPIFGRYINDKVADVIDALALYSLVVGMASSLGTGALTMLGGVSQFIDIERNAVTLGIFIAMIVVTF
LVSAASGLTKGIARLSAANFWMLIALLVFVFICGPTVYILSIGVEGLGAYLQNFFRLSLFTGQAANDPWPQYWSVFYWAV
WFAWAPITAMFLGKIARGYTVKEFIQMNVIYPSLFILVWVSVFSGTAVHMDTETNGGLNDILSNGGIEQLLYYIVHQLPF
GGVTSFFLVLVAFISYVTAADSSTDAIGDLCTKGFNSDSEDGTSLPIKMLWGTIIGLVAWIMVSTVGIDGVKQLSNLGGL
PATIIILCCSLTLIYWIRKPEMLTGKK

Sequences:

>Translated_507_residues
MLKSIRPLVFFPTFLVLVAALILSLVDLKSFTALTSAANSFVLDKFSWLFSFGSFYLLVLIVITYFSKISDVRIGGENSV
PRISKPRWFMIVLCTTLAVGVLFWTTAEPIYHLHTPPESLGIEPGSPNAILFAFSAMFLHWGPTPYAIYAVPALIFALTF
YNLKLPFSITSPLRPIFGRYINDKVADVIDALALYSLVVGMASSLGTGALTMLGGVSQFIDIERNAVTLGIFIAMIVVTF
LVSAASGLTKGIARLSAANFWMLIALLVFVFICGPTVYILSIGVEGLGAYLQNFFRLSLFTGQAANDPWPQYWSVFYWAV
WFAWAPITAMFLGKIARGYTVKEFIQMNVIYPSLFILVWVSVFSGTAVHMDTETNGGLNDILSNGGIEQLLYYIVHQLPF
GGVTSFFLVLVAFISYVTAADSSTDAIGDLCTKGFNSDSEDGTSLPIKMLWGTIIGLVAWIMVSTVGIDGVKQLSNLGGL
PATIIILCCSLTLIYWIRKPEMLTGKK
>Mature_507_residues
MLKSIRPLVFFPTFLVLVAALILSLVDLKSFTALTSAANSFVLDKFSWLFSFGSFYLLVLIVITYFSKISDVRIGGENSV
PRISKPRWFMIVLCTTLAVGVLFWTTAEPIYHLHTPPESLGIEPGSPNAILFAFSAMFLHWGPTPYAIYAVPALIFALTF
YNLKLPFSITSPLRPIFGRYINDKVADVIDALALYSLVVGMASSLGTGALTMLGGVSQFIDIERNAVTLGIFIAMIVVTF
LVSAASGLTKGIARLSAANFWMLIALLVFVFICGPTVYILSIGVEGLGAYLQNFFRLSLFTGQAANDPWPQYWSVFYWAV
WFAWAPITAMFLGKIARGYTVKEFIQMNVIYPSLFILVWVSVFSGTAVHMDTETNGGLNDILSNGGIEQLLYYIVHQLPF
GGVTSFFLVLVAFISYVTAADSSTDAIGDLCTKGFNSDSEDGTSLPIKMLWGTIIGLVAWIMVSTVGIDGVKQLSNLGGL
PATIIILCCSLTLIYWIRKPEMLTGKK

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=501, Percent_Identity=28.3433133732535, Blast_Score=197, Evalue=1e-51,
Organism=Escherichia coli, GI1786224, Length=509, Percent_Identity=25.3438113948919, Blast_Score=152, Evalue=7e-38,
Organism=Escherichia coli, GI1788102, Length=446, Percent_Identity=26.457399103139, Blast_Score=140, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 55519; Mature: 55519

Theoretical pI: Translated: 7.45; Mature: 7.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKSIRPLVFFPTFLVLVAALILSLVDLKSFTALTSAANSFVLDKFSWLFSFGSFYLLVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVITYFSKISDVRIGGENSVPRISKPRWFMIVLCTTLAVGVLFWTTAEPIYHLHTPPESL
HHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHC
GIEPGSPNAILFAFSAMFLHWGPTPYAIYAVPALIFALTFYNLKLPFSITSPLRPIFGRY
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHH
INDKVADVIDALALYSLVVGMASSLGTGALTMLGGVSQFIDIERNAVTLGIFIAMIVVTF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVSAASGLTKGIARLSAANFWMLIALLVFVFICGPTVYILSIGVEGLGAYLQNFFRLSLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHE
TGQAANDPWPQYWSVFYWAVWFAWAPITAMFLGKIARGYTVKEFIQMNVIYPSLFILVWV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SVFSGTAVHMDTETNGGLNDILSNGGIEQLLYYIVHQLPFGGVTSFFLVLVAFISYVTAA
HHHCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
DSSTDAIGDLCTKGFNSDSEDGTSLPIKMLWGTIIGLVAWIMVSTVGIDGVKQLSNLGGL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
PATIIILCCSLTLIYWIRKPEMLTGKK
HHHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLKSIRPLVFFPTFLVLVAALILSLVDLKSFTALTSAANSFVLDKFSWLFSFGSFYLLVL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IVITYFSKISDVRIGGENSVPRISKPRWFMIVLCTTLAVGVLFWTTAEPIYHLHTPPESL
HHHHHHHHHHCEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHC
GIEPGSPNAILFAFSAMFLHWGPTPYAIYAVPALIFALTFYNLKLPFSITSPLRPIFGRY
CCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCHHHHHHHH
INDKVADVIDALALYSLVVGMASSLGTGALTMLGGVSQFIDIERNAVTLGIFIAMIVVTF
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
LVSAASGLTKGIARLSAANFWMLIALLVFVFICGPTVYILSIGVEGLGAYLQNFFRLSLF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHE
TGQAANDPWPQYWSVFYWAVWFAWAPITAMFLGKIARGYTVKEFIQMNVIYPSLFILVWV
ECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
SVFSGTAVHMDTETNGGLNDILSNGGIEQLLYYIVHQLPFGGVTSFFLVLVAFISYVTAA
HHHCCCEEEECCCCCCCHHHHHCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHC
DSSTDAIGDLCTKGFNSDSEDGTSLPIKMLWGTIIGLVAWIMVSTVGIDGVKQLSNLGGL
CCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCC
PATIIILCCSLTLIYWIRKPEMLTGKK
HHHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]