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The map label for this gene is dcuB [H]

Identifier: 209694771

GI number: 209694771

Start: 1335288

End: 1336601

Strand: Direct

Name: dcuB [H]

Synonym: VSAL_I1227

Alternate gene names: 209694771

Gene position: 1335288-1336601 (Clockwise)

Preceding gene: 209694769

Following gene: 209694772

Centisome position: 40.16

GC content: 41.7

Gene sequence:

>1314_bases
GTGTTTTTAATTGAGTTTTTAATCGTTCTAGTTTGTATTTTAATTGGTGCTCGTATTGGCGGCATCGGCTTAGGTGTCAT
GGGAGGTATTGGTTTAGCAATACTGAGTTTTGTTTTTGGATTACAACCAACAAGTCCACCTATTGACGTAATGTTAATGA
TCATGGCGGTTGTTGCAGCTGCTGCAGCGATGCAAGCGGCGGGCGGTCTTGAATATTTAATTAAGATTGCATCACGTATT
TTACGTAAAAATCCACGTCACATTACCTTTATTGCTCCTTTAGTGACGTACATGTTTACTTTCTTAGCAGGTACAGGGCA
CGTTGCTTATTCTGTATTGCCTGTTATTTCAGAAGTGAGCCGTCGTAGTGGTATTCGCCCAGAACGTCCGCTTGGTATGG
CGGTTATCGCATCACAATTTGCTATTGTAGCAAGTCCGATCGCGGCAGCGGTTGTTGCTTTGGTTGCTTTTTTAGAGCCG
CAGGGAATTACTCTTGCTGATGTACTAATGATTACGATCCCAGGAACTTTCTGTGGTTTAGCACTGGCTTGCGTCATCGT
AAACAAGCTAGGTAAGGATCTAAAAGACGATCCTGAATATCAGCGTCGTATGCAAGATCCTGTCTTTCGTAAGGAAATGG
AAGCAGAAGTTAAAGTTGAAGAGATTGAGATTAAACCGGGCGCTAAAAAAGCCGTGGGTTTATTCTTATTTGGCGCAATT
GTGGTTGTTATTATGGGCGCGTTCCCATCATTACGTCCTAACTTTAATGGCTCACCTATGGGAATGGCACATACGATTGA
GATCGTCATGTTAGCCATGGCAGCGTTGATTATTTTACTTTGTAAGCCTGATGGTAATGAAATAACCCAAGGTTCGGTAT
TCCATGCGGGTATGCGTGCAATTGTGGCTATCTTTGGTATTGCATGGTTGGGTGATACGTTTATTGCGGGTCACGACATT
ATGGTAAAAGAAGCGGTTTCAGGGTTAGTTGAAGTAGCGCCATGGACATTTGCTTTTGCATTATTTGGCTTATCAGTAAT
GGTAAACAGCCAAGGGGCAACCACTGCGGTACTTGTGCCTGTTGCGATTACTTTGGGTCTTCCACCTGAAGTGATTATAG
CTACTTTTGTTGCTGTAAATGGTTATTTCTTTATTCCTAACTACGGTCCAATTATCGCTTCTATTGATTTTGACCGAACA
GGAACAACCAAGATTGGTAAGTATATCTTTAACCACAGTTTCATGATCCCTGGTTTATTGAGTATGGTTTTTAGTATCAT
ATTTGGTTTTGTTTTTGCGAGTATGGTTCTATAG

Upstream 100 bases:

>100_bases
CCTGATTATTATCCTCTCGATTTTTGGAGTTTAAATTACGCTCAATTAACGTCGTAATTTAAAAAATAGTTATATAAAAG
CTATCATCAGGGAGATTGAT

Downstream 100 bases:

>100_bases
TCATTAATTATAGCCATTAAACTGTACGTCGTACTTATTTGTTGTTATTATGCACGGGAATGAAGTATTAATGGTTTCCA
TTACTAACTTCGTCTTTGGC

Product: anaerobic C4-dicarboxylate transporter

Products: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MFLIEFLIVLVCILIGARIGGIGLGVMGGIGLAILSFVFGLQPTSPPIDVMLMIMAVVAAAAAMQAAGGLEYLIKIASRI
LRKNPRHITFIAPLVTYMFTFLAGTGHVAYSVLPVISEVSRRSGIRPERPLGMAVIASQFAIVASPIAAAVVALVAFLEP
QGITLADVLMITIPGTFCGLALACVIVNKLGKDLKDDPEYQRRMQDPVFRKEMEAEVKVEEIEIKPGAKKAVGLFLFGAI
VVVIMGAFPSLRPNFNGSPMGMAHTIEIVMLAMAALIILLCKPDGNEITQGSVFHAGMRAIVAIFGIAWLGDTFIAGHDI
MVKEAVSGLVEVAPWTFAFALFGLSVMVNSQGATTAVLVPVAITLGLPPEVIIATFVAVNGYFFIPNYGPIIASIDFDRT
GTTKIGKYIFNHSFMIPGLLSMVFSIIFGFVFASMVL

Sequences:

>Translated_437_residues
MFLIEFLIVLVCILIGARIGGIGLGVMGGIGLAILSFVFGLQPTSPPIDVMLMIMAVVAAAAAMQAAGGLEYLIKIASRI
LRKNPRHITFIAPLVTYMFTFLAGTGHVAYSVLPVISEVSRRSGIRPERPLGMAVIASQFAIVASPIAAAVVALVAFLEP
QGITLADVLMITIPGTFCGLALACVIVNKLGKDLKDDPEYQRRMQDPVFRKEMEAEVKVEEIEIKPGAKKAVGLFLFGAI
VVVIMGAFPSLRPNFNGSPMGMAHTIEIVMLAMAALIILLCKPDGNEITQGSVFHAGMRAIVAIFGIAWLGDTFIAGHDI
MVKEAVSGLVEVAPWTFAFALFGLSVMVNSQGATTAVLVPVAITLGLPPEVIIATFVAVNGYFFIPNYGPIIASIDFDRT
GTTKIGKYIFNHSFMIPGLLSMVFSIIFGFVFASMVL
>Mature_437_residues
MFLIEFLIVLVCILIGARIGGIGLGVMGGIGLAILSFVFGLQPTSPPIDVMLMIMAVVAAAAAMQAAGGLEYLIKIASRI
LRKNPRHITFIAPLVTYMFTFLAGTGHVAYSVLPVISEVSRRSGIRPERPLGMAVIASQFAIVASPIAAAVVALVAFLEP
QGITLADVLMITIPGTFCGLALACVIVNKLGKDLKDDPEYQRRMQDPVFRKEMEAEVKVEEIEIKPGAKKAVGLFLFGAI
VVVIMGAFPSLRPNFNGSPMGMAHTIEIVMLAMAALIILLCKPDGNEITQGSVFHAGMRAIVAIFGIAWLGDTFIAGHDI
MVKEAVSGLVEVAPWTFAFALFGLSVMVNSQGATTAVLVPVAITLGLPPEVIIATFVAVNGYFFIPNYGPIIASIDFDRT
GTTKIGKYIFNHSFMIPGLLSMVFSIIFGFVFASMVL

Specific function: Responsible for the transport of C4-dicarboxylates from the periplasm across the inner membrane [H]

COG id: COG2704

COG function: function code R; Anaerobic C4-dicarboxylate transporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dcuA/dcuB transporter (TC 2.A.13.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790580, Length=439, Percent_Identity=41.9134396355353, Blast_Score=333, Evalue=2e-92,
Organism=Escherichia coli, GI1790565, Length=405, Percent_Identity=41.7283950617284, Blast_Score=310, Evalue=1e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004668 [H]

Pfam domain/function: PF03605 DcuA_DcuB [H]

EC number: NA

Molecular weight: Translated: 46525; Mature: 46525

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLIEFLIVLVCILIGARIGGIGLGVMGGIGLAILSFVFGLQPTSPPIDVMLMIMAVVAA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AAAMQAAGGLEYLIKIASRILRKNPRHITFIAPLVTYMFTFLAGTGHVAYSVLPVISEVS
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RRSGIRPERPLGMAVIASQFAIVASPIAAAVVALVAFLEPQGITLADVLMITIPGTFCGL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
ALACVIVNKLGKDLKDDPEYQRRMQDPVFRKEMEAEVKVEEIEIKPGAKKAVGLFLFGAI
HHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHH
VVVIMGAFPSLRPNFNGSPMGMAHTIEIVMLAMAALIILLCKPDGNEITQGSVFHAGMRA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHH
IVAIFGIAWLGDTFIAGHDIMVKEAVSGLVEVAPWTFAFALFGLSVMVNSQGATTAVLVP
HHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHH
VAITLGLPPEVIIATFVAVNGYFFIPNYGPIIASIDFDRTGTTKIGKYIFNHSFMIPGLL
HHHHCCCCHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHH
SMVFSIIFGFVFASMVL
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MFLIEFLIVLVCILIGARIGGIGLGVMGGIGLAILSFVFGLQPTSPPIDVMLMIMAVVAA
CHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHH
AAAMQAAGGLEYLIKIASRILRKNPRHITFIAPLVTYMFTFLAGTGHVAYSVLPVISEVS
HHHHHHHCCHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
RRSGIRPERPLGMAVIASQFAIVASPIAAAVVALVAFLEPQGITLADVLMITIPGTFCGL
HHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHH
ALACVIVNKLGKDLKDDPEYQRRMQDPVFRKEMEAEVKVEEIEIKPGAKKAVGLFLFGAI
HHHHHHHHHHCCCCCCCHHHHHHHHCHHHHHHHHCCEEEEEEEECCCCHHHHHHHHHHHH
VVVIMGAFPSLRPNFNGSPMGMAHTIEIVMLAMAALIILLCKPDGNEITQGSVFHAGMRA
HHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCHHHHHHHHH
IVAIFGIAWLGDTFIAGHDIMVKEAVSGLVEVAPWTFAFALFGLSVMVNSQGATTAVLVP
HHHHHHHHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHH
VAITLGLPPEVIIATFVAVNGYFFIPNYGPIIASIDFDRTGTTKIGKYIFNHSFMIPGLL
HHHHCCCCHHHHHHHHHHHCCEEECCCCCCEEEEEECCCCCCHHHHHHHHCCCCHHHHHH
SMVFSIIFGFVFASMVL
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: C4-dicarboxylates [Periplasm]; C4-dicarboxylates [Periplasm] [C]

Specific reaction: C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] = C4-dicarboxylates [Periplasm] + C4-dicarboxylates [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]