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The map label for this gene is gadB [H]

Identifier: 209694743

GI number: 209694743

Start: 1304769

End: 1306163

Strand: Direct

Name: gadB [H]

Synonym: VSAL_I1197

Alternate gene names: 209694743

Gene position: 1304769-1306163 (Clockwise)

Preceding gene: 209694742

Following gene: 209694744

Centisome position: 39.24

GC content: 42.51

Gene sequence:

>1395_bases
ATGCCTCTTCATTCTAAAGATACAGTCAGAGACGATCTTCTTGATGATGTTTATTCATCAACTGACCTTTCCCTATCTAT
GCCAAAATACAAAATGCCTGAGCACGAACATGATCCTCGTCATGCTTATCAAGTTGTTCATGATGAGCTAATGATGGATG
GGAATTCTCGACAGAATTTAGCGACCTTTTGCCAAACTTGGGTTGAAGATGAAGTCCACAAACTAATGGACGAATGCATC
GATAAAAACATGATCGATAAGGATGAATATCCTCAAACCGCAGAGCTTGAAGCTCGTTGTGTGCATATGTTAGCTGATTT
ATGGAACTCTCCAGATGCAGAAAATACGTTAGGTTGTTCTACTACGGGGTCAAGTGAAGCCGCAATGCTCGGCGGAATGG
CGCTAAAATGGGCTTGGCGTGAAAAAATGAAAAAACTTGGCAAACCAACAGACAAACCAAATATGATCTGTGGTCCTGTT
CAAGTGTGCTGGCATAAATTTGCTCGTTATTGGGATATTGAATTACGTGAGATCCCAATGGAAGGCGATCGTCTAATTAT
GACGCCTGAAGAAGTGATTAAACGTTGTGATGAAAATACCATCGGTGTAGTTCCAACGTTGGGTGTGACATTTACTTGTC
AGTATGAACCAGTAAAAGCAGTGCATGAAGCGTTAGATAAACTACAAGAAGAAACTGGTTTAGATATTCCAATGCACATT
GATGCGGCGAGTGGCGGTTTCTTAGCCCCATTCTGTGATCCTGAGTTAGAGTGGGATTTCCGTTTACCTCGTGTGAAATC
AATCAATGCGTCAGGCCATAAATTTGGTTTGAGTCCATTAGGTGTGGGTTGGGTAATATGGCGTGATGCATCTGCATTGC
ACGAAGATCTTATTTTTAACGTGAATTACTTAGGTGGCAACATGCCTACTTTCGCGTTGAACTTCTCTCGCCCTGGCGGC
CAAATTGTAGCGCAATACTATAATTTCTTACGTTTAGGTAAAGAAGGGTACCGTAAGATCCACCAAGCGTGTTACGACAC
GGCGGTGTATCTTTCTGCTGAAATCGAAAAAATGGGCATGTTTGACATTATTTATGATGGTAAAGGTGGTATCCCTGCAA
TGAGTTGGTCGCTTAAAGAAGGCGTGAACCCTGGGTTTAACTTGTTTGATCTTTCAGACCGAATTCGTTCTCGCGGTTGG
CAAATTGCCGCTTATGCAATGCCACCAAAACGTGAAGACTTGGTGATTATGCGTATTTTGGTTCGTCATGGCTTTAGCCG
AGATCAAGCTGATCTACTGGTTGCCGATCTGAAACATTGCATCGAGTTTTTCGATAAGCACCCAATCTCCCATGGTAGTG
ATGAAGTTGAATCTTCAGGCTTTAATCACGGTTAA

Upstream 100 bases:

>100_bases
TAATTGACACTGTTGTTAATTATTTACTCATTAAGGCCGAATCCAATCAATTCGACAGTTTATATTTTATTGTTTGTTTA
ATTCAATGGAGCTAAAACGT

Downstream 100 bases:

>100_bases
TATTTTTTGGGCCGTTTTTACGGCCCATTTTTTTAATGCGCTTCTATCAATATCAGTAGAAGCCTGCTCCATCTTTATTC
TATATGGGAAATAATTATGA

Product: glutamate decarboxylase beta

Products: NA

Alternate protein names: GAD-alpha [H]

Number of amino acids: Translated: 464; Mature: 463

Protein sequence:

>464_residues
MPLHSKDTVRDDLLDDVYSSTDLSLSMPKYKMPEHEHDPRHAYQVVHDELMMDGNSRQNLATFCQTWVEDEVHKLMDECI
DKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPV
QVCWHKFARYWDIELREIPMEGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
DAASGGFLAPFCDPELEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSAEIEKMGMFDIIYDGKGGIPAMSWSLKEGVNPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCIEFFDKHPISHGSDEVESSGFNHG

Sequences:

>Translated_464_residues
MPLHSKDTVRDDLLDDVYSSTDLSLSMPKYKMPEHEHDPRHAYQVVHDELMMDGNSRQNLATFCQTWVEDEVHKLMDECI
DKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPV
QVCWHKFARYWDIELREIPMEGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
DAASGGFLAPFCDPELEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFNVNYLGGNMPTFALNFSRPGG
QIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSAEIEKMGMFDIIYDGKGGIPAMSWSLKEGVNPGFNLFDLSDRIRSRGW
QIAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCIEFFDKHPISHGSDEVESSGFNHG
>Mature_463_residues
PLHSKDTVRDDLLDDVYSSTDLSLSMPKYKMPEHEHDPRHAYQVVHDELMMDGNSRQNLATFCQTWVEDEVHKLMDECID
KNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCSTTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPVQ
VCWHKFARYWDIELREIPMEGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHID
AASGGFLAPFCDPELEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFNVNYLGGNMPTFALNFSRPGGQ
IVAQYYNFLRLGKEGYRKIHQACYDTAVYLSAEIEKMGMFDIIYDGKGGIPAMSWSLKEGVNPGFNLFDLSDRIRSRGWQ
IAAYAMPPKREDLVIMRILVRHGFSRDQADLLVADLKHCIEFFDKHPISHGSDEVESSGFNHG

Specific function: Converts glutamate to gamma-aminobutyrate (GABA), consuming one intracellular proton in the reaction. The gad system helps to maintain a near-neutral intracellular pH when cells are exposed to extremely acidic conditions. The ability to survive transit th

COG id: COG0076

COG function: function code E; Glutamate decarboxylase and related PLP-dependent proteins

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the group II decarboxylase family [H]

Homologues:

Organism=Homo sapiens, GI31982936, Length=378, Percent_Identity=25.1322751322751, Blast_Score=101, Evalue=2e-21,
Organism=Escherichia coli, GI1787769, Length=459, Percent_Identity=56.6448801742919, Blast_Score=564, Evalue=1e-162,
Organism=Escherichia coli, GI1789934, Length=458, Percent_Identity=56.7685589519651, Blast_Score=563, Evalue=1e-161,
Organism=Caenorhabditis elegans, GI17557272, Length=376, Percent_Identity=26.063829787234, Blast_Score=114, Evalue=8e-26,
Organism=Caenorhabditis elegans, GI17543922, Length=341, Percent_Identity=24.9266862170088, Blast_Score=104, Evalue=9e-23,
Organism=Caenorhabditis elegans, GI25148342, Length=321, Percent_Identity=22.4299065420561, Blast_Score=86, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323905, Length=460, Percent_Identity=38.695652173913, Blast_Score=311, Evalue=2e-85,
Organism=Saccharomyces cerevisiae, GI6320500, Length=335, Percent_Identity=25.0746268656716, Blast_Score=87, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21355963, Length=372, Percent_Identity=26.3440860215054, Blast_Score=105, Evalue=7e-23,
Organism=Drosophila melanogaster, GI24654344, Length=372, Percent_Identity=26.3440860215054, Blast_Score=105, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010107
- InterPro:   IPR002129
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR021115 [H]

Pfam domain/function: PF00282 Pyridoxal_deC [H]

EC number: =4.1.1.15 [H]

Molecular weight: Translated: 52654; Mature: 52522

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHSKDTVRDDLLDDVYSSTDLSLSMPKYKMPEHEHDPRHAYQVVHDELMMDGNSRQNL
CCCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
ATFCQTWVEDEVHKLMDECIDKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
DAASGGFLAPFCDPELEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCHHHHHHHHEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSAEIEKMGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEHHHHHCCC
FDIIYDGKGGIPAMSWSLKEGVNPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEECCCCCCCCCCCCHHHCCCCCCCEEEHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCIEFFDKHPISHGSDEVESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure 
PLHSKDTVRDDLLDDVYSSTDLSLSMPKYKMPEHEHDPRHAYQVVHDELMMDGNSRQNL
CCCCCCHHHHHHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCHHH
ATFCQTWVEDEVHKLMDECIDKNMIDKDEYPQTAELEARCVHMLADLWNSPDAENTLGCS
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCC
TTGSSEAAMLGGMALKWAWREKMKKLGKPTDKPNMICGPVQVCWHKFARYWDIELREIPM
CCCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHCCCEEEECCC
EGDRLIMTPEEVIKRCDENTIGVVPTLGVTFTCQYEPVKAVHEALDKLQEETGLDIPMHI
CCCEEEECHHHHHHHCCCCCCEEEECCCEEEEECCCHHHHHHHHHHHHHHHHCCCCEEEE
DAASGGFLAPFCDPELEWDFRLPRVKSINASGHKFGLSPLGVGWVIWRDASALHEDLIFN
ECCCCCEECCCCCCCCCCCCCCCCEEECCCCCCEECCCCCCCCEEEECCHHHHHHHHEEE
VNYLGGNMPTFALNFSRPGGQIVAQYYNFLRLGKEGYRKIHQACYDTAVYLSAEIEKMGM
EEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHEEHHHHHCCC
FDIIYDGKGGIPAMSWSLKEGVNPGFNLFDLSDRIRSRGWQIAAYAMPPKREDLVIMRIL
EEEEECCCCCCCCCCCCHHHCCCCCCCEEEHHHHHHHCCCEEEEEECCCCCCHHHHHHHH
VRHGFSRDQADLLVADLKHCIEFFDKHPISHGSDEVESSGFNHG
HHCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]