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The map label for this gene is menH [H]

Identifier: 209694658

GI number: 209694658

Start: 1197062

End: 1197856

Strand: Direct

Name: menH [H]

Synonym: VSAL_I1100

Alternate gene names: 209694658

Gene position: 1197062-1197856 (Clockwise)

Preceding gene: 209694657

Following gene: 209694659

Centisome position: 36.0

GC content: 41.38

Gene sequence:

>795_bases
ATGCCACTTTATTCTAAATTGATTGAACCCATAGAAAGCACTACACAACCTTGTGTGGTGTTTTTACATGGACTATTGGG
CTCAATAAAAGAGTGGGATCAGATAGCCTCCGTCATTGCTCAATCACACCCTGTATTATTAATTGACCTTCCTGGACACG
GTAAAAGTCAGTCGGTGGAATTGGAAGAGTCAACCGGGTTTGAACAAGCATGCCAGCTTATTATTGAGCAGTGTCACAGT
TCTCCATATCAAGAGTTTGTGCTTGTCGGTTACTCTTTAGGAGGGCGTTTGGCGATGTATCTGAGTGCTTTTTTTCAGCT
TCCTAATGACATAACCCTCCGAGGTTTGTGCGTTGAAGGAGGGAACTTTGGTTTAATCTCAAAAGAAGAAAAGCACATTC
GGTGGCAAAATGACACGATGTGGGCAGAACGATTTGAACAACAACCGATTGCTGATGTTTTAGACAATTGGTACCAACAA
AAGGTATTTTCTTCACTAAACCCTGAGCAAAGACAAGTTTTAGTAGCAAAGAGAAGTGATAATCTTGGACTCTCGATTGG
AATGATGTTGAGATCAACCTCACTGGCTAAGCAACCATATTTGCTTAACGAGCTGCATCGTTGCACCATCCCTATGCTTT
ATATTTGCGGTGAAGCAGGTCAAAAATTTCAACACTTGGCTGAGCAAAGTCAATTAACGTATCAAATCATTGCTCAGGCT
GGTCATAACGCTCATGTGGAACAGCCCGAACGCTTTACACATGTACTTAATACATTCTTACAACAATGGACATAA

Upstream 100 bases:

>100_bases
ATCAATGACGGCTTAAAAGGCGGCGTTCATTTAGTTGAAATTAATACACCGTCAGGTCAAGCAGGCGAAGAATTAACACG
ATTATTCCAAACCATTCAAC

Downstream 100 bases:

>100_bases
CCCCATGGCAAGAACTGTTGGCATTTCAGAAGAAGAACTTTACCCTCCAGTAAATTGGACAGATTGCACTGCGCAATATG
AAGACATTCAATATCATAAA

Product: acyl-CoA thioester hydrolase YfbB

Products: NA

Alternate protein names: SHCHC synthase [H]

Number of amino acids: Translated: 264; Mature: 263

Protein sequence:

>264_residues
MPLYSKLIEPIESTTQPCVVFLHGLLGSIKEWDQIASVIAQSHPVLLIDLPGHGKSQSVELEESTGFEQACQLIIEQCHS
SPYQEFVLVGYSLGGRLAMYLSAFFQLPNDITLRGLCVEGGNFGLISKEEKHIRWQNDTMWAERFEQQPIADVLDNWYQQ
KVFSSLNPEQRQVLVAKRSDNLGLSIGMMLRSTSLAKQPYLLNELHRCTIPMLYICGEAGQKFQHLAEQSQLTYQIIAQA
GHNAHVEQPERFTHVLNTFLQQWT

Sequences:

>Translated_264_residues
MPLYSKLIEPIESTTQPCVVFLHGLLGSIKEWDQIASVIAQSHPVLLIDLPGHGKSQSVELEESTGFEQACQLIIEQCHS
SPYQEFVLVGYSLGGRLAMYLSAFFQLPNDITLRGLCVEGGNFGLISKEEKHIRWQNDTMWAERFEQQPIADVLDNWYQQ
KVFSSLNPEQRQVLVAKRSDNLGLSIGMMLRSTSLAKQPYLLNELHRCTIPMLYICGEAGQKFQHLAEQSQLTYQIIAQA
GHNAHVEQPERFTHVLNTFLQQWT
>Mature_263_residues
PLYSKLIEPIESTTQPCVVFLHGLLGSIKEWDQIASVIAQSHPVLLIDLPGHGKSQSVELEESTGFEQACQLIIEQCHSS
PYQEFVLVGYSLGGRLAMYLSAFFQLPNDITLRGLCVEGGNFGLISKEEKHIRWQNDTMWAERFEQQPIADVLDNWYQQK
VFSSLNPEQRQVLVAKRSDNLGLSIGMMLRSTSLAKQPYLLNELHRCTIPMLYICGEAGQKFQHLAEQSQLTYQIIAQAG
HNAHVEQPERFTHVLNTFLQQWT

Specific function: Catalyzes a proton abstraction reaction that results in 2,5-elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6- hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). Is also

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. MenH family [H]

Homologues:

Organism=Escherichia coli, GI1788598, Length=236, Percent_Identity=41.5254237288136, Blast_Score=173, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR022485 [H]

Pfam domain/function: PF00561 Abhydrolase_1 [H]

EC number: =4.2.99.20 [H]

Molecular weight: Translated: 29987; Mature: 29855

Theoretical pI: Translated: 5.76; Mature: 5.76

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLYSKLIEPIESTTQPCVVFLHGLLGSIKEWDQIASVIAQSHPVLLIDLPGHGKSQSVE
CCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEE
LEESTGFEQACQLIIEQCHSSPYQEFVLVGYSLGGRLAMYLSAFFQLPNDITLRGLCVEG
CHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
GNFGLISKEEKHIRWQNDTMWAERFEQQPIADVLDNWYQQKVFSSLNPEQRQVLVAKRSD
CCCCCCCCCHHHCEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHEEEEEECCC
NLGLSIGMMLRSTSLAKQPYLLNELHRCTIPMLYICGEAGQKFQHLAEQSQLTYQIIAQA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GHNAHVEQPERFTHVLNTFLQQWT
CCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PLYSKLIEPIESTTQPCVVFLHGLLGSIKEWDQIASVIAQSHPVLLIDLPGHGKSQSVE
CHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCEE
LEESTGFEQACQLIIEQCHSSPYQEFVLVGYSLGGRLAMYLSAFFQLPNDITLRGLCVEG
CHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEC
GNFGLISKEEKHIRWQNDTMWAERFEQQPIADVLDNWYQQKVFSSLNPEQRQVLVAKRSD
CCCCCCCCCHHHCEECCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHEEEEEECCC
NLGLSIGMMLRSTSLAKQPYLLNELHRCTIPMLYICGEAGQKFQHLAEQSQLTYQIIAQA
CCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHC
GHNAHVEQPERFTHVLNTFLQQWT
CCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA