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The map label for this gene is mltB [H]

Identifier: 209694628

GI number: 209694628

Start: 1159825

End: 1160799

Strand: Direct

Name: mltB [H]

Synonym: VSAL_I1069

Alternate gene names: 209694628

Gene position: 1159825-1160799 (Clockwise)

Preceding gene: 209694627

Following gene: 209694630

Centisome position: 34.88

GC content: 40.51

Gene sequence:

>975_bases
ATGAAGAAAAGATTACTGCTAGGGATAGTCAGTTTGTTAATGGCCACAAGCGTATCTGCGAATACGCCTGAAGGGGATTT
CCAGGATTATATCGTTAACTTAAAAAATGAAGCGCGCGAGAATGGTATTTCAGAGCCTATTTTAGAACGAGCGTTTAAAA
ATGTGGCTTTTAAAGAAAAAGCGGTTAAATCTGATCGGAATCAGCCAGAAAAAAAATTAACACTAGATGAATATATTCCA
CGAGCAGTACCAAAGTGGAAGATAAAACAAGCGAACGATTTATATCAAAAACACCATGAAGTGCTTACTAGAATTGGCAA
AGAATATGGGGTTCAACCTCGATTTATCGTCGCATTATGGGGTGTTGAAAGTAATTTTGGTAAGCTAACGGGGGGTTATA
ATGTGATAGAAGCATTAACTACGTTGGCTTATGATGGTCGCCGTGAAGCCTTTTTTAGAAAGCAAACAATGGCGGCATTA
ACCATTTTAGAACAAGGTCACATTACCCCTGAAAACATGAAAGGTTCATGGGCAGGTGCAATGGGGCAATGCCAGTTTAT
GCCAACGTCATTTTTGGCTTATGCTGTTGATGGCAATGATGATGGTCGTAAAGATATTTGGACGACTCATGCCGATGTGT
TCGCTTCTGCGGCGAATTACTTAAAACAAGTGGGTTGGGATGATACGTATACTTGGGGTCGTCAGGTAACATTGCCTGAT
GCTTTTGATGCCGAAGCCCTAAAGGGACTTTCTGAAGATAAAGGAAAGTTACTCTCTGAATGGGGGGCATTAGGGGTTCG
TCGTTTAAATGGAAAGTCGTTACCTAATGTTGAGTTAGAAGCATGGTTAGTGCAACCAGACGATAATGATGGTCGTGCTT
ACCTTGTTTATAATAACTATCAAGTGTTAATGGATTGGAATCGCTCGCATTATTTTGCATTAGCGGTGAGCCACTTGGCT
GATTCTATTAAGTAA

Upstream 100 bases:

>100_bases
TCTTTAAAAAATGACGGCTATTTTTTACAATTACCGCCACCTCCTGAAAATTTGCTTGAGAAATACAAGCAAGATAAAGC
CAAACGAAACGAGAAATAAT

Downstream 100 bases:

>100_bases
AAGATCAAGATTTAGGTAGCTGTGATCAGAGGAGTTCGTTATGAGTTTATCATTTACAACGAACTCCCCTGAAACGGGTA
AGAGAGCTTGCGACCGAGCT

Product: hypothetical protein

Products: Muramic Acid Residue [C]

Alternate protein names: 35 kDa soluble lytic transglycosylase; Murein hydrolase B; Slt35 [H]

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MKKRLLLGIVSLLMATSVSANTPEGDFQDYIVNLKNEARENGISEPILERAFKNVAFKEKAVKSDRNQPEKKLTLDEYIP
RAVPKWKIKQANDLYQKHHEVLTRIGKEYGVQPRFIVALWGVESNFGKLTGGYNVIEALTTLAYDGRREAFFRKQTMAAL
TILEQGHITPENMKGSWAGAMGQCQFMPTSFLAYAVDGNDDGRKDIWTTHADVFASAANYLKQVGWDDTYTWGRQVTLPD
AFDAEALKGLSEDKGKLLSEWGALGVRRLNGKSLPNVELEAWLVQPDDNDGRAYLVYNNYQVLMDWNRSHYFALAVSHLA
DSIK

Sequences:

>Translated_324_residues
MKKRLLLGIVSLLMATSVSANTPEGDFQDYIVNLKNEARENGISEPILERAFKNVAFKEKAVKSDRNQPEKKLTLDEYIP
RAVPKWKIKQANDLYQKHHEVLTRIGKEYGVQPRFIVALWGVESNFGKLTGGYNVIEALTTLAYDGRREAFFRKQTMAAL
TILEQGHITPENMKGSWAGAMGQCQFMPTSFLAYAVDGNDDGRKDIWTTHADVFASAANYLKQVGWDDTYTWGRQVTLPD
AFDAEALKGLSEDKGKLLSEWGALGVRRLNGKSLPNVELEAWLVQPDDNDGRAYLVYNNYQVLMDWNRSHYFALAVSHLA
DSIK
>Mature_324_residues
MKKRLLLGIVSLLMATSVSANTPEGDFQDYIVNLKNEARENGISEPILERAFKNVAFKEKAVKSDRNQPEKKLTLDEYIP
RAVPKWKIKQANDLYQKHHEVLTRIGKEYGVQPRFIVALWGVESNFGKLTGGYNVIEALTTLAYDGRREAFFRKQTMAAL
TILEQGHITPENMKGSWAGAMGQCQFMPTSFLAYAVDGNDDGRKDIWTTHADVFASAANYLKQVGWDDTYTWGRQVTLPD
AFDAEALKGLSEDKGKLLSEWGALGVRRLNGKSLPNVELEAWLVQPDDNDGRAYLVYNNYQVLMDWNRSHYFALAVSHLA
DSIK

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG2951

COG function: function code M; Membrane-bound lytic murein transglycosylase B

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor; Periplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1789053, Length=232, Percent_Identity=31.8965517241379, Blast_Score=100, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011757 [H]

Pfam domain/function: NA

EC number: 3.2.1.- [C]

Molecular weight: Translated: 36572; Mature: 36572

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKRLLLGIVSLLMATSVSANTPEGDFQDYIVNLKNEARENGISEPILERAFKNVAFKEK
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AVKSDRNQPEKKLTLDEYIPRAVPKWKIKQANDLYQKHHEVLTRIGKEYGVQPRFIVALW
HHHHCCCCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GVESNFGKLTGGYNVIEALTTLAYDGRREAFFRKQTMAALTILEQGHITPENMKGSWAGA
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MGQCQFMPTSFLAYAVDGNDDGRKDIWTTHADVFASAANYLKQVGWDDTYTWGRQVTLPD
CCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
AFDAEALKGLSEDKGKLLSEWGALGVRRLNGKSLPNVELEAWLVQPDDNDGRAYLVYNNY
CCCHHHHCCCCHHHHHHHHHHHHHCEEECCCCCCCCCEEEEEEECCCCCCCEEEEEECCE
QVLMDWNRSHYFALAVSHLADSIK
EEEEECCCCEEHHHHHHHHHHHCC
>Mature Secondary Structure
MKKRLLLGIVSLLMATSVSANTPEGDFQDYIVNLKNEARENGISEPILERAFKNVAFKEK
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
AVKSDRNQPEKKLTLDEYIPRAVPKWKIKQANDLYQKHHEVLTRIGKEYGVQPRFIVALW
HHHHCCCCCHHHCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEE
GVESNFGKLTGGYNVIEALTTLAYDGRREAFFRKQTMAALTILEQGHITPENMKGSWAGA
CCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
MGQCQFMPTSFLAYAVDGNDDGRKDIWTTHADVFASAANYLKQVGWDDTYTWGRQVTLPD
CCCEEECCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCC
AFDAEALKGLSEDKGKLLSEWGALGVRRLNGKSLPNVELEAWLVQPDDNDGRAYLVYNNY
CCCHHHHCCCCHHHHHHHHHHHHHCEEECCCCCCCCCEEEEEEECCCCCCCEEEEEECCE
QVLMDWNRSHYFALAVSHLADSIK
EEEEECCCCEEHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

General reaction: Cleavage Bond [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 7476170; 7789526; 9205837; 9278503; 3553176; 9761817; 10570954; 10545329; 10684641 [H]