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The map label for this gene is mtnN [H]

Identifier: 209694615

GI number: 209694615

Start: 1146709

End: 1147524

Strand: Direct

Name: mtnN [H]

Synonym: VSAL_I1055

Alternate gene names: 209694615

Gene position: 1146709-1147524 (Clockwise)

Preceding gene: 209694614

Following gene: 209694619

Centisome position: 34.49

GC content: 39.58

Gene sequence:

>816_bases
ATGAAAGGCTTTCGTTGTTGTTTTATTGCTTTAACTATGATGTTTTCATTTTCGGCAAGTGCAATGCCACAACCCATTCT
TCTTCAAGGCGCAATGGATATTGAAGTTGAGTATATGGTTGCTCAATTGGAAAATAAAAAAGAAGAGACGATTGGGTCTT
GGACTTTTTGGGAAGGAACAATTGACGGCTATCCAGTGGTTGTCTCAAGAACGGAAGTTGGTATTGCTAATGCTTCAGCC
TCTACCGTACTTGGGATTGAAAAGTTTTCACCAAGTATGATTGTTAACCAAGGTACATCGGGTGGACATGATCCTGAATT
GTATCGAGGTGATATTGTTTTAGCTGTAAAGAGTTTCAATATGGGGGCAAACCGCTCTGAGTTTTCTGAAGCGAGTGCTG
GTATTCAACCTGAAAAATGGAAAAATTTTACCGTTACTATGCGTCTTAGAGAAGATAACAAGCTAGTTGAGCATGATGCA
TTTTACTCTGAACCAACATTAGTTGATTTTGCTTTCGAGAATGTAAGTCACCCAGGTAAAGGTAAGGTAGTGAAGGGCGT
GATTGGAACTGCTGATGAATGGAATCGCGAGGTGGCGAGAATTAACTGGTTGCATAAAACATATCAAACTTCAGCTGAAG
AGATGGAAAGTTCTTCTGCTGCGTTAGTTGCTAAGGCTTACAATATTCCGTTTATAGGTATTCGTATTTTGTCAAACACT
GACCTACACAATCAAGATTTTGATCCTGAGACCGCAACAGTGAATCAGAAGTACGTGATTGATTTTGTGAAAAAGTGGGT
TGCGAGCCAAAAATAA

Upstream 100 bases:

>100_bases
CTTTTATAGATAAAAGCAATCGTTTGCTTATGTTGTCTATTCATGTATTATCCGCCGCATATTTTCGTGTAACAATGTAA
TTCAAACAGGGTATAAATAT

Downstream 100 bases:

>100_bases
ATTTACTTTATATAACGAAAAATCCCCAGCATTTCTGCTAGGGCTTCGAAATTGGTGGAGGGATAGGGATTTGAACCCTA
GAACCGCTATTAACGGTTGC

Product: phosphorylase

Products: NA

Alternate protein names: MTA/SAH nucleosidase; MTAN; 5'-methylthioadenosine nucleosidase; MTA nucleosidase; S-adenosylhomocysteine nucleosidase; AdoHcy nucleosidase; SAH nucleosidase; SRH nucleosidase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MKGFRCCFIALTMMFSFSASAMPQPILLQGAMDIEVEYMVAQLENKKEETIGSWTFWEGTIDGYPVVVSRTEVGIANASA
STVLGIEKFSPSMIVNQGTSGGHDPELYRGDIVLAVKSFNMGANRSEFSEASAGIQPEKWKNFTVTMRLREDNKLVEHDA
FYSEPTLVDFAFENVSHPGKGKVVKGVIGTADEWNREVARINWLHKTYQTSAEEMESSSAALVAKAYNIPFIGIRILSNT
DLHNQDFDPETATVNQKYVIDFVKKWVASQK

Sequences:

>Translated_271_residues
MKGFRCCFIALTMMFSFSASAMPQPILLQGAMDIEVEYMVAQLENKKEETIGSWTFWEGTIDGYPVVVSRTEVGIANASA
STVLGIEKFSPSMIVNQGTSGGHDPELYRGDIVLAVKSFNMGANRSEFSEASAGIQPEKWKNFTVTMRLREDNKLVEHDA
FYSEPTLVDFAFENVSHPGKGKVVKGVIGTADEWNREVARINWLHKTYQTSAEEMESSSAALVAKAYNIPFIGIRILSNT
DLHNQDFDPETATVNQKYVIDFVKKWVASQK
>Mature_271_residues
MKGFRCCFIALTMMFSFSASAMPQPILLQGAMDIEVEYMVAQLENKKEETIGSWTFWEGTIDGYPVVVSRTEVGIANASA
STVLGIEKFSPSMIVNQGTSGGHDPELYRGDIVLAVKSFNMGANRSEFSEASAGIQPEKWKNFTVTMRLREDNKLVEHDA
FYSEPTLVDFAFENVSHPGKGKVVKGVIGTADEWNREVARINWLHKTYQTSAEEMESSSAALVAKAYNIPFIGIRILSNT
DLHNQDFDPETATVNQKYVIDFVKKWVASQK

Specific function: Catalyzes the irreversible cleavage of the glycosidic bond in both 5'-methylthioadenosine (MTA) and S- adenosylhomocysteine (SAH/AdoHcy) to adenine and the corresponding thioribose, 5'-methylthioribose and S-ribosylhomocysteine, respectively [H]

COG id: COG0775

COG function: function code F; Nucleoside phosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PNP/UDP phosphorylase family. MtnN subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786354, Length=212, Percent_Identity=25.4716981132075, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010049
- InterPro:   IPR018017
- InterPro:   IPR000845 [H]

Pfam domain/function: PF01048 PNP_UDP_1 [H]

EC number: =3.2.2.9 [H]

Molecular weight: Translated: 30201; Mature: 30201

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGFRCCFIALTMMFSFSASAMPQPILLQGAMDIEVEYMVAQLENKKEETIGSWTFWEGT
CCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEHHHHHHHHHCCHHHHCCCEEEECCC
IDGYPVVVSRTEVGIANASASTVLGIEKFSPSMIVNQGTSGGHDPELYRGDIVLAVKSFN
CCCEEEEEEECEECEECCCCCEEEEEECCCCCEEEECCCCCCCCCCEEECCEEEEEEECC
MGANRSEFSEASAGIQPEKWKNFTVTMRLREDNKLVEHDAFYSEPTLVDFAFENVSHPGK
CCCCHHHHHHHHCCCCCHHCCCEEEEEEECCCCCEECCCCCCCCCCEEEEEHHCCCCCCC
GKVVKGVIGTADEWNREVARINWLHKTYQTSAEEMESSSAALVAKAYNIPFIGIRILSNT
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECC
DLHNQDFDPETATVNQKYVIDFVKKWVASQK
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKGFRCCFIALTMMFSFSASAMPQPILLQGAMDIEVEYMVAQLENKKEETIGSWTFWEGT
CCCHHHHHHHHHHHHHCCCCCCCCCEEEECCCCEEHHHHHHHHHCCHHHHCCCEEEECCC
IDGYPVVVSRTEVGIANASASTVLGIEKFSPSMIVNQGTSGGHDPELYRGDIVLAVKSFN
CCCEEEEEEECEECEECCCCCEEEEEECCCCCEEEECCCCCCCCCCEEECCEEEEEEECC
MGANRSEFSEASAGIQPEKWKNFTVTMRLREDNKLVEHDAFYSEPTLVDFAFENVSHPGK
CCCCHHHHHHHHCCCCCHHCCCEEEEEEECCCCCEECCCCCCCCCCEEEEEHHCCCCCCC
GKVVKGVIGTADEWNREVARINWLHKTYQTSAEEMESSSAALVAKAYNIPFIGIRILSNT
CCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEEEECC
DLHNQDFDPETATVNQKYVIDFVKKWVASQK
CCCCCCCCCCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA