Click here to switch to the map view.

The map label for this gene is miaB

Identifier: 209694559

GI number: 209694559

Start: 1100110

End: 1101534

Strand: Reverse

Name: miaB

Synonym: VSAL_I0996

Alternate gene names: 209694559

Gene position: 1101534-1100110 (Counterclockwise)

Preceding gene: 209694563

Following gene: 209694558

Centisome position: 33.13

GC content: 40.7

Gene sequence:

>1425_bases
ATGACTAAGAAACTGCTAATCAAAACCTGGGGCTGCCAGATGAACGAATACGATTCATCTAAAATGGCCGATCTTTTAGG
TGCCGCAAATGGTTATGAATTAACTGAAGATCCAAAAGAAGCGGATGTTCTGTTATTAAATACCTGTTCAATTCGTGAAA
AAGCACAAGAAAAAGTGTTTCACCAACTGGGCCGCTGGAAACATTTAAAAGACAAAAAACCAGACTTAGTGATTGGCGTA
GGCGGTTGTGTTGCAACTCAAGAAGGCGATCATATTCGTCAACGTGCACCTTACGTTGATGTAATTTTTGGTCCTCAAAC
GCTTCACCGTTTACCACAAATGATCAAACAATCGCTTTCTAATGAAAAAACCGTTATGGATATTTCATTTCCTGAAATCG
AAAAATTCGATAATCTTCCAGAACCAAAAGCCGATGGTGTATCTGCTTTTGTTTCTATCATGGAAGGTTGTTCAAAATAC
TGTACGTACTGTGTTGTTCCTTATACTCGCGGTGAAGAAGTTAGCCGTCCATTAGATGACGTGCTATTTGAAATTGCTCA
ACTTGCAGAGCAAGGCGTACGTGAAGTTAACCTATTAGGTCAAAACGTAAATGCTTACCGTGGTCCTATGTACGATGGTG
ATATTTGTTCCTTTGCTGATTTACTTCGTATGGTTGCTTCAATTGATGGTATTGACCGTTTACGCTTCACAACCAGTCAC
CCATTAGAATTTGGTGATGATATTGTTGCGGTTTACGAAGATACACCAGAATTAGTAAGCTTCTTGCATCTACCTGTTCA
AAGTGGCTCAGATCGAATCCTAACGATGATGAAACGTCCTCATACTGCTATTGAGTACAAGTCAATCATCCGTAAGTTGC
GTAAAGCTCGTCCTGACATTTTAATCAGCTCAGATTTCATCGTTGCCTTCCCTGGTGAGTCAGAAAAAGACTTCCAAGAT
ACAATGAAGCTAATTAAAGACGTAGACTTTGACATGAGTTATAGCTTTGTCTTCTCTCCACGTCCAGGTACGCCAGCCGC
TGATTATCCGTGTGATATTCCAGAGCAAACGAAAAAAGATCGCCTTGCTGAGCTTCAACAACAGATCAGTTTTCAATCTG
CACGCTTCTCTCGTCAGATGTTAAATACAGAGCTACGTGCACTTGTTGAAGGCCCATCAAAAAGAAATCCGATGGAACTA
CGAGCTCGTACTGAAAATAACCGCGTAGTGAATTTCACAGGCAGTCCAGAACTCATTGGACAATTTGTTGACCTTCATAT
TACAGAATCATTTACTAACTCTTTACGCGGTACCTTAGTTCGTACCGAAAAAGACATGGGATTACGTGTTGCTGTATCAC
CTTCTGAGATGATAGAGAAAACTCGCCGTGAAGATGATCTTGGTGTAGGTACTTTTACGCCATAA

Upstream 100 bases:

>100_bases
GCTTGGAAAGCAGTACAATACGCGCCCTATCCGTTTTGATTGAGCTTGTCTCTCAAAACAGTACCGAATTCTAGTAATAA
AATACGAGTGAAAAAGTTAC

Downstream 100 bases:

>100_bases
TGTGATCACCTCAGTAAAATCAAACACTAAAAAAGATGATTAAGCTTACTTGATCATCTTTTTTCTTATTTAACCTTGAG
AAAACCCCCTTTTTCCCCAA

Product: (dimethylallyl)adenosine tRNA methylthiotransferase

Products: NA

Alternate protein names: tRNA-i(6)A37 methylthiotransferase

Number of amino acids: Translated: 474; Mature: 473

Protein sequence:

>474_residues
MTKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEDPKEADVLLLNTCSIREKAQEKVFHQLGRWKHLKDKKPDLVIGV
GGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPQMIKQSLSNEKTVMDISFPEIEKFDNLPEPKADGVSAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPLDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPMYDGDICSFADLLRMVASIDGIDRLRFTTSH
PLEFGDDIVAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDILISSDFIVAFPGESEKDFQD
TMKLIKDVDFDMSYSFVFSPRPGTPAADYPCDIPEQTKKDRLAELQQQISFQSARFSRQMLNTELRALVEGPSKRNPMEL
RARTENNRVVNFTGSPELIGQFVDLHITESFTNSLRGTLVRTEKDMGLRVAVSPSEMIEKTRREDDLGVGTFTP

Sequences:

>Translated_474_residues
MTKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEDPKEADVLLLNTCSIREKAQEKVFHQLGRWKHLKDKKPDLVIGV
GGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPQMIKQSLSNEKTVMDISFPEIEKFDNLPEPKADGVSAFVSIMEGCSKY
CTYCVVPYTRGEEVSRPLDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPMYDGDICSFADLLRMVASIDGIDRLRFTTSH
PLEFGDDIVAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDILISSDFIVAFPGESEKDFQD
TMKLIKDVDFDMSYSFVFSPRPGTPAADYPCDIPEQTKKDRLAELQQQISFQSARFSRQMLNTELRALVEGPSKRNPMEL
RARTENNRVVNFTGSPELIGQFVDLHITESFTNSLRGTLVRTEKDMGLRVAVSPSEMIEKTRREDDLGVGTFTP
>Mature_473_residues
TKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEDPKEADVLLLNTCSIREKAQEKVFHQLGRWKHLKDKKPDLVIGVG
GCVATQEGDHIRQRAPYVDVIFGPQTLHRLPQMIKQSLSNEKTVMDISFPEIEKFDNLPEPKADGVSAFVSIMEGCSKYC
TYCVVPYTRGEEVSRPLDDVLFEIAQLAEQGVREVNLLGQNVNAYRGPMYDGDICSFADLLRMVASIDGIDRLRFTTSHP
LEFGDDIVAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDILISSDFIVAFPGESEKDFQDT
MKLIKDVDFDMSYSFVFSPRPGTPAADYPCDIPEQTKKDRLAELQQQISFQSARFSRQMLNTELRALVEGPSKRNPMELR
ARTENNRVVNFTGSPELIGQFVDLHITESFTNSLRGTLVRTEKDMGLRVAVSPSEMIEKTRREDDLGVGTFTP

Specific function: Catalyzes the methylthiolation of N6- (dimethylallyl)adenosine (i(6)A), leading to the formation of 2- methylthio-N6-(dimethylallyl)adenosine (ms(2)i(6)A) at position 37 in tRNAs that read codons beginning with uridine

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=480, Percent_Identity=34.375, Blast_Score=272, Evalue=5e-73,
Organism=Homo sapiens, GI28872782, Length=480, Percent_Identity=34.375, Blast_Score=271, Evalue=1e-72,
Organism=Homo sapiens, GI93277076, Length=457, Percent_Identity=28.8840262582057, Blast_Score=182, Evalue=6e-46,
Organism=Escherichia coli, GI1786882, Length=474, Percent_Identity=73.6286919831224, Blast_Score=737, Evalue=0.0,
Organism=Escherichia coli, GI1787057, Length=391, Percent_Identity=28.6445012787724, Blast_Score=165, Evalue=5e-42,
Organism=Caenorhabditis elegans, GI17553146, Length=463, Percent_Identity=30.8855291576674, Blast_Score=228, Evalue=7e-60,
Organism=Caenorhabditis elegans, GI71996771, Length=251, Percent_Identity=30.2788844621514, Blast_Score=115, Evalue=5e-26,
Organism=Drosophila melanogaster, GI21356207, Length=478, Percent_Identity=34.1004184100418, Blast_Score=271, Evalue=1e-72,
Organism=Drosophila melanogaster, GI19922432, Length=380, Percent_Identity=31.0526315789474, Blast_Score=157, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MIAB_ALISL (B6EIN8)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262487.1
- GeneID:   6986768
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0996
- HOGENOM:   HBG457663
- OMA:   ANLHDDT
- ProtClustDB:   PRK14325
- GO:   GO:0005737
- HAMAP:   MF_01864
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR006463
- InterPro:   IPR007197
- InterPro:   IPR002792
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01574
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF01938 TRAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 53567; Mature: 53436

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEDPKEADVLLLNTCSIREKAQEKVF
CCCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKHLKDKKPDLVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPQMIKQSLS
HHHHCCCCCCCCCCCEEEECCCEEECCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHC
NEKTVMDISFPEIEKFDNLPEPKADGVSAFVSIMEGCSKYCTYCVVPYTRGEEVSRPLDD
CCCEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPMYDGDICSFADLLRMVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PLEFGDDIVAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCE
LISSDFIVAFPGESEKDFQDTMKLIKDVDFDMSYSFVFSPRPGTPAADYPCDIPEQTKKD
EEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
RLAELQQQISFQSARFSRQMLNTELRALVEGPSKRNPMELRARTENNRVVNFTGSPELIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCHHHHH
QFVDLHITESFTNSLRGTLVRTEKDMGLRVAVSPSEMIEKTRREDDLGVGTFTP
HHHHHEEHHHHHHHHHHEEEEECCCCCEEEEECHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TKKLLIKTWGCQMNEYDSSKMADLLGAANGYELTEDPKEADVLLLNTCSIREKAQEKVF
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHH
HQLGRWKHLKDKKPDLVIGVGGCVATQEGDHIRQRAPYVDVIFGPQTLHRLPQMIKQSLS
HHHHCCCCCCCCCCCEEEECCCEEECCCCCHHHHHCCEEEEEECHHHHHHHHHHHHHHHC
NEKTVMDISFPEIEKFDNLPEPKADGVSAFVSIMEGCSKYCTYCVVPYTRGEEVSRPLDD
CCCEEEEECCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEEECCCCCHHCCCHHH
VLFEIAQLAEQGVREVNLLGQNVNAYRGPMYDGDICSFADLLRMVASIDGIDRLRFTTSH
HHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCHHHHHHHHHHHHHCCCCHHEEECCCC
PLEFGDDIVAVYEDTPELVSFLHLPVQSGSDRILTMMKRPHTAIEYKSIIRKLRKARPDI
CCCCCCCEEEEECCCHHHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHCCCCE
LISSDFIVAFPGESEKDFQDTMKLIKDVDFDMSYSFVFSPRPGTPAADYPCDIPEQTKKD
EEECCEEEECCCCCCHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCCHHHHH
RLAELQQQISFQSARFSRQMLNTELRALVEGPSKRNPMELRARTENNRVVNFTGSPELIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEEECCCHHHHH
QFVDLHITESFTNSLRGTLVRTEKDMGLRVAVSPSEMIEKTRREDDLGVGTFTP
HHHHHEEHHHHHHHHHHEEEEECCCCCEEEEECHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA