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The map label for this gene is proB

Identifier: 209694540

GI number: 209694540

Start: 1079931

End: 1081040

Strand: Direct

Name: proB

Synonym: VSAL_I0977

Alternate gene names: 209694540

Gene position: 1079931-1081040 (Clockwise)

Preceding gene: 209694539

Following gene: 209694541

Centisome position: 32.48

GC content: 41.08

Gene sequence:

>1110_bases
TTGAATACACAATCTCAAACCATAGTCGTTAAACTTGGTACAAGTGTACTCACTGGTGGCACATTAAAACTAGACAGAGC
ACACATGGTTGAATTAGTTCGCCAATGTGTGCATCTGAAAAAGTTAGGTCATCAAGTGATTGTTGTAACTTCTGGCGCAA
TTGCGGCTGGACGTGAACATTTAAATTACCCCGAACTACCCAAAACCATGGCTAACAAGCAGTTATTAGCTGCTGTTGGT
CAAAGTTGCTTAATTCAAGCTTGGCAGAGTTTATTTGCTATTTATGGTGTTAACGTTGGCCAAATGTTATTAACTCGAGC
GGATTTAGATGATCGTGAACGTTATCTAAATGCCCGTGACATGCTGCAAGCTTTATTAAAAAATGGCATTGTTCCTATCG
TTAATGAGAATGATGCGGTTGCAACAAATGAAATTAAGGTTGGGGATAACGATAACCTTTCTGCTTTAGTTGGTATTTTG
GCGAGTGCAGATAAATTATTGCTGTTAACGGATCAATCTGGTTTGTTTACGGCTGATCCGCGTAAAGATCCAAAAGCCGA
ATTGATTAAAGAAGTGCACACCATTGATGAAACACTACGTAAGATTGCAGGTGGTAGTGGGACGGCATTAGGCACCGGTG
GCATGGCAACTAAACTTCAAGCGGCTGATATTGCTCGTCGAGCGGGTATTGAAGTTGTTATTGCTGCTGGCAGCGCTGAG
AATGTGATTACTGATGTTGTAGATTCAAAGCCTCAAGGAACAAAATTCTTGCCTGTTGAATGTGCGTTAGAGAGTCGTAA
ACGTTGGATTCTTGCTGGTCCTGCATCTAATGGTACGATTGTTATTGATGACGGTGCTGTGAATGCAGTACAGCAAAAAG
GCAGCAGTTTATTGAGCAAAGGGATTTCGTCGATTTCTGGCCACTTTGTTCGCGGTGGTGTTGCTAAGATTGTAAATACG
AAAGGTGAGTTAATTGCCCGAGGTATTTCTCGTTATTCAAGTGACGATTTACATAAGATTTTAGGAAAACACAGTCAGGA
TATTTACGCAGTATTAGGTTATGAATATGGGCCTGTAGCTATCCACCGAGATGATTTAGTACTGATTTAG

Upstream 100 bases:

>100_bases
TCTGATTTGCTTGCTAAAAATAGGGTGATTTTTATATTATTATTACGATGAATGTATTTCGTAATACAAAACATTATTAG
TTAATATAGGTAGTAGGGAA

Downstream 100 bases:

>100_bases
TAAAGGATATTTATTGTGAATTTAACAAAAATGGGTCAAGCAGCAAAAAATGCAGCGTTTGACTTAGCGGTAGCTCCAAC
AGCCCAGAAGAACAAAGCAT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVHLKKLGHQVIVVTSGAIAAGREHLNYPELPKTMANKQLLAAVG
QSCLIQAWQSLFAIYGVNVGQMLLTRADLDDRERYLNARDMLQALLKNGIVPIVNENDAVATNEIKVGDNDNLSALVGIL
ASADKLLLLTDQSGLFTADPRKDPKAELIKEVHTIDETLRKIAGGSGTALGTGGMATKLQAADIARRAGIEVVIAAGSAE
NVITDVVDSKPQGTKFLPVECALESRKRWILAGPASNGTIVIDDGAVNAVQQKGSSLLSKGISSISGHFVRGGVAKIVNT
KGELIARGISRYSSDDLHKILGKHSQDIYAVLGYEYGPVAIHRDDLVLI

Sequences:

>Translated_369_residues
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVHLKKLGHQVIVVTSGAIAAGREHLNYPELPKTMANKQLLAAVG
QSCLIQAWQSLFAIYGVNVGQMLLTRADLDDRERYLNARDMLQALLKNGIVPIVNENDAVATNEIKVGDNDNLSALVGIL
ASADKLLLLTDQSGLFTADPRKDPKAELIKEVHTIDETLRKIAGGSGTALGTGGMATKLQAADIARRAGIEVVIAAGSAE
NVITDVVDSKPQGTKFLPVECALESRKRWILAGPASNGTIVIDDGAVNAVQQKGSSLLSKGISSISGHFVRGGVAKIVNT
KGELIARGISRYSSDDLHKILGKHSQDIYAVLGYEYGPVAIHRDDLVLI
>Mature_369_residues
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVHLKKLGHQVIVVTSGAIAAGREHLNYPELPKTMANKQLLAAVG
QSCLIQAWQSLFAIYGVNVGQMLLTRADLDDRERYLNARDMLQALLKNGIVPIVNENDAVATNEIKVGDNDNLSALVGIL
ASADKLLLLTDQSGLFTADPRKDPKAELIKEVHTIDETLRKIAGGSGTALGTGGMATKLQAADIARRAGIEVVIAAGSAE
NVITDVVDSKPQGTKFLPVECALESRKRWILAGPASNGTIVIDDGAVNAVQQKGSSLLSKGISSISGHFVRGGVAKIVNT
KGELIARGISRYSSDDLHKILGKHSQDIYAVLGYEYGPVAIHRDDLVLI

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=294, Percent_Identity=32.9931972789116, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI62912457, Length=292, Percent_Identity=32.8767123287671, Blast_Score=128, Evalue=8e-30,
Organism=Escherichia coli, GI1786437, Length=365, Percent_Identity=66.027397260274, Blast_Score=492, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17569969, Length=270, Percent_Identity=34.0740740740741, Blast_Score=135, Evalue=3e-32,
Organism=Caenorhabditis elegans, GI17569967, Length=270, Percent_Identity=34.0740740740741, Blast_Score=135, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6321822, Length=425, Percent_Identity=36.2352941176471, Blast_Score=223, Evalue=4e-59,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=35.799522673031, Blast_Score=214, Evalue=2e-56,
Organism=Drosophila melanogaster, GI21357643, Length=308, Percent_Identity=33.7662337662338, Blast_Score=127, Evalue=2e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_ALISL (B6EIF1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262468.1
- ProteinModelPortal:   B6EIF1
- GeneID:   6986759
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0977
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 39304; Mature: 39304

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVHLKKLGHQVIVVTSGAIAAGREH
CCCCCCEEEEEECCCEECCCCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHH
LNYPELPKTMANKQLLAAVGQSCLIQAWQSLFAIYGVNVGQMLLTRADLDDRERYLNARD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCHHH
MLQALLKNGIVPIVNENDAVATNEIKVGDNDNLSALVGILASADKLLLLTDQSGLFTADP
HHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCC
RKDPKAELIKEVHTIDETLRKIAGGSGTALGTGGMATKLQAADIARRAGIEVVIAAGSAE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEEEEECCCCC
NVITDVVDSKPQGTKFLPVECALESRKRWILAGPASNGTIVIDDGAVNAVQQKGSSLLSK
HHHHHHHCCCCCCCEEEEHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHH
GISSISGHFVRGGVAKIVNTKGELIARGISRYSSDDLHKILGKHSQDIYAVLGYEYGPVA
HHHHHCCHHHHCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCEE
IHRDDLVLI
EECCCEEEC
>Mature Secondary Structure
MNTQSQTIVVKLGTSVLTGGTLKLDRAHMVELVRQCVHLKKLGHQVIVVTSGAIAAGREH
CCCCCCEEEEEECCCEECCCCEEECHHHHHHHHHHHHHHHHCCCEEEEEECCCHHHCHHH
LNYPELPKTMANKQLLAAVGQSCLIQAWQSLFAIYGVNVGQMLLTRADLDDRERYLNARD
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHCCHHH
MLQALLKNGIVPIVNENDAVATNEIKVGDNDNLSALVGILASADKLLLLTDQSGLFTADP
HHHHHHHCCCEEEECCCCCEEECEEEECCCCCHHHHHHHHHCCCEEEEEECCCCCEECCC
RKDPKAELIKEVHTIDETLRKIAGGSGTALGTGGMATKLQAADIARRAGIEVVIAAGSAE
CCCCHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCHHHHHHHHHHHHCCCEEEEECCCCC
NVITDVVDSKPQGTKFLPVECALESRKRWILAGPASNGTIVIDDGAVNAVQQKGSSLLSK
HHHHHHHCCCCCCCEEEEHHHHHCCCCEEEEECCCCCCEEEEECCCHHHHHHHHHHHHHH
GISSISGHFVRGGVAKIVNTKGELIARGISRYSSDDLHKILGKHSQDIYAVLGYEYGPVA
HHHHHCCHHHHCCHHHHHCCCHHHHHHHHHHCCCHHHHHHHCCCCCCEEEEECCCCCCEE
IHRDDLVLI
EECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA