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The map label for this gene is opuD [H]

Identifier: 209694451

GI number: 209694451

Start: 976947

End: 978521

Strand: Reverse

Name: opuD [H]

Synonym: VSAL_I0875

Alternate gene names: 209694451

Gene position: 978521-976947 (Counterclockwise)

Preceding gene: 209694452

Following gene: 209694450

Centisome position: 29.43

GC content: 44.32

Gene sequence:

>1575_bases
ATGTCTGATGTAACCGATAACATGAAAAGTTCAAGCAAGAATTCGTCAATAAAAGAGAATGAATCCACGGCAGAAAAACT
GGGGCTCGATAACCCCGCTTTATGGTATAGCGGTGGTTTTATCGCTTTATTTGTCTCACTTGCGTTAGTGAATGCTGAAT
TACTGTCGACCATTGTTAATAGTGGATTTGCATGGGCTGTGAGTGTCTTTGGTCCTTATTGGCAAATCTTATTATTACTG
ACATTCCTAATTGGTTTAGGGTTGGCAGCAGGACGTACTGGTCGAGTTATTTTGGGGGGAATAGATAAACCTGAAATGGA
CGGATTCCGCTGGATGGCGATCATCTTTTGTACTTTATTAGCGGGTGGAGGCGTATTTTGGGCCGCGGCTGAGCCGATAG
CTCATTTTGTTAGTGCACCACCATTATATGGCGCGCAAGAGAACGTACAGCAAGCGGCGGTTAATGCGTTATCACAATCT
TTCATGCATTGGGGATTCTTAGCATGGGCAATCGTGGGGAGCTTAACGTCCATTGTTGTTATGCATCTTCATTATGACAA
AGGGTTACCGCTTAAACCGAGAATTTTACTTTATCCTCTCTTTGGTAAACGCGTACTGACTGGCCACACAGGTGCATTGA
TTGATGCGTGTTGTATTGTCGCGGTTGCGGCAGGGACAATTGGTCCGATTGGTTTCTTAGGTCTACAGGTGAGCTATGCG
TTAAACGCTCTGTTTGATATACCAGATGGTTTTACCACACAGCTCATCATCATATTATTCGCTATCGCTCTATACACAAT
TTCTGCGTTAAGTGGTTTGAATCGTGGTATGCAAATGCTTAGCCGTTATAACGTTATTTTAGCGTCAGCATTGATGATTT
ATGTGCTTGTTTTTGGTCCAACTAACTTTATCGTCAATGGCTACATCCAAGGGGTGGGCAGCATGATTGATAATTTCATT
CCGATGGCAACCTATCGTGGTGATGAAGGCTGGTTAAGTTGGTGGACGGTATTTTTCTGGGGATGGTTCTTAGGGTACGG
ACCAATGATGGCGATTTTTATTGCTCGCATATCACGCGGCCGCAGTATTCGCCAATTGATCTCTACCATTAGTATTATTG
CACCGCTTGTGACGTGCTTTTGGTTCACGATAGTCGGTGGTTCAGGATTGGCTTTTGAAATTGCGAATCCAGGCAGTGTG
AGCTCGGCTTTTGAAGGGTTTAACTTACCGGGGGCTTTATTGGCCGTGACCTCTCAATTGCCAATGCCAATGGTAACGTC
AATCCTATTTCTAATTTTAACCACCATCTTTATTGTGACCACGGGTGACTCGATGACATACACCATCAGTGTGGTTATCA
GTGGAGAAACAGAGCCAAATGCCATTATCAGAACCTTTTGGGGCGTGATGATGGGGGTAACGGCATTAATCCTTATCTCT
CTTGGATCTGGTGGTATATCAGCATTGCAATCCTTCATTGTCATTACCGCTGTTCCTGTCTCTTTAATCTTGTTGCCCTC
ACTTTGGGATGCACCACGGATCGCCATGAGGATGGCAAAAGAACAAGGGTTGTAA

Upstream 100 bases:

>100_bases
AAAACGAAACCCATACATGGAGCACCTACTCAATACAACAATAATAGGGTAGGTAGCAAGCACACCTTTGCAGCTGCAAA
TTGAATACTGGAGCCAGAGT

Downstream 100 bases:

>100_bases
ACCCATCCAATAACGTGAAGCAAGAGCGGTGTTTGCCGCTCAACTTGGCTCTAATAATGAACTAAATTCTAATAAAAGAG
CCCCTATCAATACACCTATA

Product: glycine betaine transporter OpuD

Products: Proton [Cytoplasm]; choline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 524; Mature: 523

Protein sequence:

>524_residues
MSDVTDNMKSSSKNSSIKENESTAEKLGLDNPALWYSGGFIALFVSLALVNAELLSTIVNSGFAWAVSVFGPYWQILLLL
TFLIGLGLAAGRTGRVILGGIDKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHFVSAPPLYGAQENVQQAAVNALSQS
FMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPLFGKRVLTGHTGALIDACCIVAVAAGTIGPIGFLGLQVSYA
LNALFDIPDGFTTQLIIILFAIALYTISALSGLNRGMQMLSRYNVILASALMIYVLVFGPTNFIVNGYIQGVGSMIDNFI
PMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRGRSIRQLISTISIIAPLVTCFWFTIVGGSGLAFEIANPGSV
SSAFEGFNLPGALLAVTSQLPMPMVTSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAIIRTFWGVMMGVTALILIS
LGSGGISALQSFIVITAVPVSLILLPSLWDAPRIAMRMAKEQGL

Sequences:

>Translated_524_residues
MSDVTDNMKSSSKNSSIKENESTAEKLGLDNPALWYSGGFIALFVSLALVNAELLSTIVNSGFAWAVSVFGPYWQILLLL
TFLIGLGLAAGRTGRVILGGIDKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHFVSAPPLYGAQENVQQAAVNALSQS
FMHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPLFGKRVLTGHTGALIDACCIVAVAAGTIGPIGFLGLQVSYA
LNALFDIPDGFTTQLIIILFAIALYTISALSGLNRGMQMLSRYNVILASALMIYVLVFGPTNFIVNGYIQGVGSMIDNFI
PMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRGRSIRQLISTISIIAPLVTCFWFTIVGGSGLAFEIANPGSV
SSAFEGFNLPGALLAVTSQLPMPMVTSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAIIRTFWGVMMGVTALILIS
LGSGGISALQSFIVITAVPVSLILLPSLWDAPRIAMRMAKEQGL
>Mature_523_residues
SDVTDNMKSSSKNSSIKENESTAEKLGLDNPALWYSGGFIALFVSLALVNAELLSTIVNSGFAWAVSVFGPYWQILLLLT
FLIGLGLAAGRTGRVILGGIDKPEMDGFRWMAIIFCTLLAGGGVFWAAAEPIAHFVSAPPLYGAQENVQQAAVNALSQSF
MHWGFLAWAIVGSLTSIVVMHLHYDKGLPLKPRILLYPLFGKRVLTGHTGALIDACCIVAVAAGTIGPIGFLGLQVSYAL
NALFDIPDGFTTQLIIILFAIALYTISALSGLNRGMQMLSRYNVILASALMIYVLVFGPTNFIVNGYIQGVGSMIDNFIP
MATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRGRSIRQLISTISIIAPLVTCFWFTIVGGSGLAFEIANPGSVS
SAFEGFNLPGALLAVTSQLPMPMVTSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAIIRTFWGVMMGVTALILISL
GSGGISALQSFIVITAVPVSLILLPSLWDAPRIAMRMAKEQGL

Specific function: High-affinity uptake of glycine betaine [H]

COG id: COG1292

COG function: function code M; Choline-glycine betaine transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the BCCT transporter (TC 2.A.15) family [H]

Homologues:

Organism=Escherichia coli, GI1786506, Length=485, Percent_Identity=30.3092783505155, Blast_Score=211, Evalue=9e-56,
Organism=Escherichia coli, GI1788102, Length=426, Percent_Identity=29.1079812206573, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1786224, Length=491, Percent_Identity=24.847250509165, Blast_Score=157, Evalue=2e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000060
- InterPro:   IPR018093 [H]

Pfam domain/function: PF02028 BCCT [H]

EC number: NA

Molecular weight: Translated: 56352; Mature: 56221

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVTDNMKSSSKNSSIKENESTAEKLGLDNPALWYSGGFIALFVSLALVNAELLSTIVN
CCCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHC
SGFAWAVSVFGPYWQILLLLTFLIGLGLAAGRTGRVILGGIDKPEMDGFRWMAIIFCTLL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
AGGGVFWAAAEPIAHFVSAPPLYGAQENVQQAAVNALSQSFMHWGFLAWAIVGSLTSIVV
HCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHLHYDKGLPLKPRILLYPLFGKRVLTGHTGALIDACCIVAVAAGTIGPIGFLGLQVSYA
HHEECCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LNALFDIPDGFTTQLIIILFAIALYTISALSGLNRGMQMLSRYNVILASALMIYVLVFGP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNFIVNGYIQGVGSMIDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRG
HHHHHHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSIRQLISTISIIAPLVTCFWFTIVGGSGLAFEIANPGSVSSAFEGFNLPGALLAVTSQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHHCC
PMPMVTSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAIIRTFWGVMMGVTALILIS
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LGSGGISALQSFIVITAVPVSLILLPSLWDAPRIAMRMAKEQGL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC
>Mature Secondary Structure 
SDVTDNMKSSSKNSSIKENESTAEKLGLDNPALWYSGGFIALFVSLALVNAELLSTIVN
CCCHHHHHCCCCCCCCCCCHHHHHHHCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHC
SGFAWAVSVFGPYWQILLLLTFLIGLGLAAGRTGRVILGGIDKPEMDGFRWMAIIFCTLL
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHH
AGGGVFWAAAEPIAHFVSAPPLYGAQENVQQAAVNALSQSFMHWGFLAWAIVGSLTSIVV
HCCCEEEHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MHLHYDKGLPLKPRILLYPLFGKRVLTGHTGALIDACCIVAVAAGTIGPIGFLGLQVSYA
HHEECCCCCCCCCCEEEEECCCCEEECCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
LNALFDIPDGFTTQLIIILFAIALYTISALSGLNRGMQMLSRYNVILASALMIYVLVFGP
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
TNFIVNGYIQGVGSMIDNFIPMATYRGDEGWLSWWTVFFWGWFLGYGPMMAIFIARISRG
HHHHHHHHHHHHHHHHHCCCCHHEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
RSIRQLISTISIIAPLVTCFWFTIVGGSGLAFEIANPGSVSSAFEGFNLPGALLAVTSQL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCHHHHCCCCCCHHHHHHHHCC
PMPMVTSILFLILTTIFIVTTGDSMTYTISVVISGETEPNAIIRTFWGVMMGVTALILIS
CHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
LGSGGISALQSFIVITAVPVSLILLPSLWDAPRIAMRMAKEQGL
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; choline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + choline [Periplasm] = Proton [Cytoplasm] + choline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8752321; 9387221; 9384377 [H]