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The map label for this gene is dapF

Identifier: 209693749

GI number: 209693749

Start: 162449

End: 163279

Strand: Direct

Name: dapF

Synonym: VSAL_I0126

Alternate gene names: 209693749

Gene position: 162449-163279 (Clockwise)

Preceding gene: 209693748

Following gene: 209693750

Centisome position: 4.89

GC content: 42.6

Gene sequence:

>831_bases
ATGCACTTTCATTTTTCTAAAATGCACGGGCTAGGCAATGACTTTATGGTGGTTGATTGCTTAACTCAAAATATTTTCTT
TTCACCAGATTTGATCCGTCGTCTGGCTGACCGTAATCGTGGTATCGGTTTCGATCAGTTATTGGTGGTTGAAGCTCCCT
ATGATCCTGAAACTGATTTCCATTACCGAATTTTTAATTCTGATGGCACAGAAGTAGAGCAATGCGGCAATGGCGCTCGT
TGTTTTGCTCGTTTTGTCCGAATGAAAGGCTTAACGAATCGAACGACTATTTTGGTCAGCACGAAAAAAGGAAAAATGAG
TTTAAAGGTGGAAGATGATGATCAAATTACGGTGAACATGGGTGAACCTCAATTTGAACCAAACAAAATTCCATTTAAAG
CGACTCAAGCTGAAAAAACCTATTTATTGCGTGCGGAAGATAAAACATTATTTTGTGGTGCGGTGAGTATGGGCAATCCA
CACTGCGTGACGGTGGTGGATGATGTCGATAATTACGATGTTGATAAATACGGCCCATTAGTGGAAGGGCATGAACGCTT
CCCTGAGAGAGTAAACGCAGGCTTTATGGAGATAGTTTCTCCGAGCGAAATTAAATTACGTGTTTATGAACGTGGTGCGG
GTGAAACTCAAGCGTGTGGAAGCGGTGCTTGTGCAGCGGTTGCTGTAGGTATAATGCAAGAACTCCTAGAAGAAGACGTT
AAAGTGTCTTTGCCTGGTGGTGATTTACGTATTGTTTGGCAAGGCCCGGGTAAACCGTTATTCATGACAGGGCCTGCGAC
ACACGTTTATGACGGTCAATTATCAATATGA

Upstream 100 bases:

>100_bases
ATCTATTTTACCAAACTAACTTAGAATAAAGAGCAAGAGGCTTAGGCTGCTTGCTCTTTTGGTATTCAACCTAGAAGATA
AAATAAAAAGAAGAACAACT

Downstream 100 bases:

>100_bases
ATGAAGTAACACCAATAGAGCCACAGCCGACTCAATTAACGGAAGACGTTGTGGCTCAATTTTTAATGGATCACCCTGAT
TTTTTTCAACAGCATCCCGA

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MHFHFSKMHGLGNDFMVVDCLTQNIFFSPDLIRRLADRNRGIGFDQLLVVEAPYDPETDFHYRIFNSDGTEVEQCGNGAR
CFARFVRMKGLTNRTTILVSTKKGKMSLKVEDDDQITVNMGEPQFEPNKIPFKATQAEKTYLLRAEDKTLFCGAVSMGNP
HCVTVVDDVDNYDVDKYGPLVEGHERFPERVNAGFMEIVSPSEIKLRVYERGAGETQACGSGACAAVAVGIMQELLEEDV
KVSLPGGDLRIVWQGPGKPLFMTGPATHVYDGQLSI

Sequences:

>Translated_276_residues
MHFHFSKMHGLGNDFMVVDCLTQNIFFSPDLIRRLADRNRGIGFDQLLVVEAPYDPETDFHYRIFNSDGTEVEQCGNGAR
CFARFVRMKGLTNRTTILVSTKKGKMSLKVEDDDQITVNMGEPQFEPNKIPFKATQAEKTYLLRAEDKTLFCGAVSMGNP
HCVTVVDDVDNYDVDKYGPLVEGHERFPERVNAGFMEIVSPSEIKLRVYERGAGETQACGSGACAAVAVGIMQELLEEDV
KVSLPGGDLRIVWQGPGKPLFMTGPATHVYDGQLSI
>Mature_276_residues
MHFHFSKMHGLGNDFMVVDCLTQNIFFSPDLIRRLADRNRGIGFDQLLVVEAPYDPETDFHYRIFNSDGTEVEQCGNGAR
CFARFVRMKGLTNRTTILVSTKKGKMSLKVEDDDQITVNMGEPQFEPNKIPFKATQAEKTYLLRAEDKTLFCGAVSMGNP
HCVTVVDDVDNYDVDKYGPLVEGHERFPERVNAGFMEIVSPSEIKLRVYERGAGETQACGSGACAAVAVGIMQELLEEDV
KVSLPGGDLRIVWQGPGKPLFMTGPATHVYDGQLSI

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=70.8029197080292, Blast_Score=424, Evalue=1e-120,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPF_ALISL (B6EP85)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261677.1
- ProteinModelPortal:   B6EP85
- GeneID:   6988769
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0126
- HOGENOM:   HBG399442
- OMA:   HTGIGFD
- ProtClustDB:   PRK00450
- GO:   GO:0005737
- HAMAP:   MF_00197
- InterPro:   IPR001653
- InterPro:   IPR018510
- TIGRFAMs:   TIGR00652

Pfam domain/function: PF01678 DAP_epimerase

EC number: =5.1.1.7

Molecular weight: Translated: 30678; Mature: 30678

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: ACT_SITE 75-75 ACT_SITE 219-219

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHFHFSKMHGLGNDFMVVDCLTQNIFFSPDLIRRLADRNRGIGFDQLLVVEAPYDPETDF
CCEECHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCE
HYRIFNSDGTEVEQCGNGARCFARFVRMKGLTNRTTILVSTKKGKMSLKVEDDDQITVNM
EEEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCEEEEEC
GEPQFEPNKIPFKATQAEKTYLLRAEDKTLFCGAVSMGNPHCVTVVDDVDNYDVDKYGPL
CCCCCCCCCCCEEECCCCEEEEEEECCCEEEEEEEECCCCEEEEEEECCCCCCCHHCCCC
VEGHERFPERVNAGFMEIVSPSEIKLRVYERGAGETQACGSGACAAVAVGIMQELLEEDV
CCCHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
KVSLPGGDLRIVWQGPGKPLFMTGPATHVYDGQLSI
EEEECCCCEEEEEECCCCCEEEECCCCEEECCEECC
>Mature Secondary Structure
MHFHFSKMHGLGNDFMVVDCLTQNIFFSPDLIRRLADRNRGIGFDQLLVVEAPYDPETDF
CCEECHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCE
HYRIFNSDGTEVEQCGNGARCFARFVRMKGLTNRTTILVSTKKGKMSLKVEDDDQITVNM
EEEEECCCCCCHHHCCCHHHHHHHHHHHCCCCCCEEEEEEECCCEEEEEECCCCEEEEEC
GEPQFEPNKIPFKATQAEKTYLLRAEDKTLFCGAVSMGNPHCVTVVDDVDNYDVDKYGPL
CCCCCCCCCCCEEECCCCEEEEEEECCCEEEEEEEECCCCEEEEEEECCCCCCCHHCCCC
VEGHERFPERVNAGFMEIVSPSEIKLRVYERGAGETQACGSGACAAVAVGIMQELLEEDV
CCCHHHHHHHHCCCEEEECCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
KVSLPGGDLRIVWQGPGKPLFMTGPATHVYDGQLSI
EEEECCCCEEEEEECCCCCEEEECCCCEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA