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The map label for this gene is hflB [C]

Identifier: 209554574

GI number: 209554574

Start: 551089

End: 552144

Strand: Reverse

Name: hflB [C]

Synonym: UUR10_0500

Alternate gene names: 209554574

Gene position: 552144-551089 (Counterclockwise)

Preceding gene: 209554347

Following gene: 209553999

Centisome position: 63.14

GC content: 24.72

Gene sequence:

>1056_bases
ATGAAAAAAGTTGATATTGTTAATCTAATTAAATACCATGTTAGTAATGATGAAAATAGTTTTATTAATCAAGCTAATGA
AATTGCTAAATATTTTGATGATCATGGTGATAATGAAATTGCGAGTTATATTATGGGACTAATTAGTCGTTCTAATGTTT
TTACTCCTCAATCGTTAAAAAGTGAATTAACATCTGATTTTTTTGAAAAAGTTGATTTGCACAAATTTGAACCACTACCA
ATACCTGAAGCTATTCAAAAAGAAATAGATGGTTTAATAAATGCATATAAAAAGGGATTTGATTTAAATAAATTTTTATT
TAAAGGACCACCAGGAACTGGAAAAACTGAATCTGTTAAAAGTCTTGCACGTCTTTTGAAAAAAGAATTATATAAAGTTA
ATTTATCTTCGATTGTTGATAGTATGTTAGGAAGAACAGCTAAAAATATTAAAGAATTGTTTGATGAAATTAATGCGTTC
CCTAATCCAGAAAATGTTCTTATTTTATTAGATGAAATAGATGCTATTACAATGGATCGTATTAATAATAATGATTCACG
TGAAATGGGACGAGCAACATCAACAATTTTAACTTGTTTAGATAACTTAAATAAATCAATTTTATTAATTGCAACAACTA
ATTTACATCAATATTTTGATGATGCACTACTACGTCGATTTGATTATGCTATTGATTTTTCACATTATGAAAAAGAAGAT
ATACAAGATATTGGATGAATTATTTTAGAATACTATTCACAACGAATGGAAAATATTAATAAAAACAAAAATTTGTTTAA
GAAAATCATTAAAAATGCCAAAGTTATGCCTTATCCTGGAAAATTAAAAAATCTAATTAAATCATCATTAGCATTTTCTG
ATCCTAACGATCCTAATGAATATTTAAAACGATTATATTATGATTTGGTTGATAATCATCTAGATATTAATATACTAAAA
GAACAAGGTTTTACAGTCAGAGAAATTGAAACCTTAACTGATATTTCAAAAAGTAAAATCGCAAAAGATTTAAAGGAAAA
TAAAGATTATGAATAA

Upstream 100 bases:

>100_bases
GTTTTCTTTTTGTTTTGAAAAATTTGTCCTAAAATTGTTATATTTGTTTTTGTAATGTGTTAGAATTTTATTGCATTAAT
ATCAGGAGTAGAAATTAAAT

Downstream 100 bases:

>100_bases
TATTTTAGAATTAAAAGGTTCAATATATTCAAAATCAAATCCTAATAAACCAGGGCCACGTAATCTTCCAGCAAAACAAA
GTATTAGTTTAGATCATATT

Product: AAA family ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MKKVDIVNLIKYHVSNDENSFINQANEIAKYFDDHGDNEIASYIMGLISRSNVFTPQSLKSELTSDFFEKVDLHKFEPLP
IPEAIQKEIDGLINAYKKGFDLNKFLFKGPPGTGKTESVKSLARLLKKELYKVNLSSIVDSMLGRTAKNIKELFDEINAF
PNPENVLILLDEIDAITMDRINNNDSREMGRATSTILTCLDNLNKSILLIATTNLHQYFDDALLRRFDYAIDFSHYEKED
IQDIGWIILEYYSQRMENINKNKNLFKKIIKNAKVMPYPGKLKNLIKSSLAFSDPNDPNEYLKRLYYDLVDNHLDINILK
EQGFTVREIETLTDISKSKIAKDLKENKDYE

Sequences:

>Translated_351_residues
MKKVDIVNLIKYHVSNDENSFINQANEIAKYFDDHGDNEIASYIMGLISRSNVFTPQSLKSELTSDFFEKVDLHKFEPLP
IPEAIQKEIDGLINAYKKGFDLNKFLFKGPPGTGKTESVKSLARLLKKELYKVNLSSIVDSMLGRTAKNIKELFDEINAF
PNPENVLILLDEIDAITMDRINNNDSREMGRATSTILTCLDNLNKSILLIATTNLHQYFDDALLRRFDYAIDFSHYEKED
IQDIG*IILEYYSQRMENINKNKNLFKKIIKNAKVMPYPGKLKNLIKSSLAFSDPNDPNEYLKRLYYDLVDNHLDINILK
EQGFTVREIETLTDISKSKIAKDLKENKDYE
>Mature_351_residues
MKKVDIVNLIKYHVSNDENSFINQANEIAKYFDDHGDNEIASYIMGLISRSNVFTPQSLKSELTSDFFEKVDLHKFEPLP
IPEAIQKEIDGLINAYKKGFDLNKFLFKGPPGTGKTESVKSLARLLKKELYKVNLSSIVDSMLGRTAKNIKELFDEINAF
PNPENVLILLDEIDAITMDRINNNDSREMGRATSTILTCLDNLNKSILLIATTNLHQYFDDALLRRFDYAIDFSHYEKED
IQDIG*IILEYYSQRMENINKNKNLFKKIIKNAKVMPYPGKLKNLIKSSLAFSDPNDPNEYLKRLYYDLVDNHLDINILK
EQGFTVREIETLTDISKSKIAKDLKENKDYE

Specific function: Seems To Act As An ATP-Dependent Zinc Metallopeptidase. Involved In The Degradation Of Sigma-32. Degrades Carboxy- Terminal-Tagged Cytoplasmic Proteins. These Proteins Are Tagged With An 11-Amino-Acid Nonpolar Destabilizing Tail VIa A Mechanism Involving

COG id: COG0464

COG function: function code O; ATPases of the AAA+ class

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AAA ATPase family [H]

Homologues:

Organism=Caenorhabditis elegans, GI71987372, Length=135, Percent_Identity=35.5555555555556, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI71987364, Length=135, Percent_Identity=35.5555555555556, Blast_Score=68, Evalue=8e-12,
Organism=Caenorhabditis elegans, GI17508701, Length=165, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17533043, Length=198, Percent_Identity=26.2626262626263, Blast_Score=65, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6323429, Length=134, Percent_Identity=32.089552238806, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6322994, Length=135, Percent_Identity=37.037037037037, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6320602, Length=134, Percent_Identity=35.0746268656716, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6324000, Length=129, Percent_Identity=32.5581395348837, Blast_Score=65, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6320077, Length=135, Percent_Identity=32.5925925925926, Blast_Score=64, Evalue=4e-11,
Organism=Saccharomyces cerevisiae, GI9755327, Length=215, Percent_Identity=27.906976744186, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI28573181, Length=164, Percent_Identity=30.4878048780488, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640100, Length=134, Percent_Identity=35.8208955223881, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19920408, Length=193, Percent_Identity=33.160621761658, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR003959 [H]

Pfam domain/function: PF00004 AAA [H]

EC number: 3.4.24.- [C]

Molecular weight: Translated: 40339; Mature: 40339

Theoretical pI: Translated: 5.53; Mature: 5.53

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVDIVNLIKYHVSNDENSFINQANEIAKYFDDHGDNEIASYIMGLISRSNVFTPQSLK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHH
SELTSDFFEKVDLHKFEPLPIPEAIQKEIDGLINAYKKGFDLNKFLFKGPPGTGKTESVK
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCHHHHH
SLARLLKKELYKVNLSSIVDSMLGRTAKNIKELFDEINAFPNPENVLILLDEIDAITMDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
INNNDSREMGRATSTILTCLDNLNKSILLIATTNLHQYFDDALLRRFDYAIDFSHYEKED
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCHHCCHHH
IQDIGIILEYYSQRMENINKNKNLFKKIIKNAKVMPYPGKLKNLIKSSLAFSDPNDPNEY
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
LKRLYYDLVDNHLDINILKEQGFTVREIETLTDISKSKIAKDLKENKDYE
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKKVDIVNLIKYHVSNDENSFINQANEIAKYFDDHGDNEIASYIMGLISRSNVFTPQSLK
CCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCHHHHH
SELTSDFFEKVDLHKFEPLPIPEAIQKEIDGLINAYKKGFDLNKFLFKGPPGTGKTESVK
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHEECCCCCCCHHHHH
SLARLLKKELYKVNLSSIVDSMLGRTAKNIKELFDEINAFPNPENVLILLDEIDAITMDR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHHHHH
INNNDSREMGRATSTILTCLDNLNKSILLIATTNLHQYFDDALLRRFDYAIDFSHYEKED
CCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCHHCCHHH
IQDIGIILEYYSQRMENINKNKNLFKKIIKNAKVMPYPGKLKNLIKSSLAFSDPNDPNEY
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCCCHHHH
LKRLYYDLVDNHLDINILKEQGFTVREIETLTDISKSKIAKDLKENKDYE
HHHHHHHHHCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Metalloendopeptidases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]